Shedding light on microRNA function via microscopy-based screening.
AuthorsRodrigues Lopes I, Silva RJ, Caramelo I, Eulalio A, Mano M,
JournalMethods
PubMed ID30292796
'MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally modulate gene expression and orchestrate a wide range of biological and pathological processes. The use of high-throughput screening technologies, in particular microscopy-based screenings (also known as high-content screenings), coupled with genome-wide libraries for modulation of miRNA levels, allow for comprehensive functional analysis ... More
Competitive inhibition by NAG-1/GDF-15 NLS peptide enhances its anti-cancer activity.
AuthorsLee J, Kim I, Yoo E, Baek SJ,
JournalBiochem Biophys Res Commun
PubMed ID31474335
Non-steroidal anti-inflammatory drug activated gene-1 (NAG-1), also known as growth differentiation factor 15 (GDF15), is a TGF-ß (transforming growth factor beta) superfamily protein with a distinctive secretion pathway. NAG-1 is associated with multiple diseases including cancer, wherein it plays a role in both pro- and anti-cancer activities. We previously reported ... More