1-Dimethylaminonaphthalene-5-sulfonyl hydrazine (dansyl hydrazine): a fluorometric reagent for carbonyl compounds.
AuthorsChayes R, Dvir R, Gould S, Harell A
JournalAnal Biochem
PubMed ID4253984
Micro method for detection of formyl and acetyl groups in proteins.
AuthorsSchmer G, Kreil G
JournalAnal Biochem
PubMed ID5815580
Characterization of the major structural proteins of purified bovine viral diarrhea virus.
AuthorsCoria MF, Schmerr MJ, McClurkin AW
JournalArch Virol
PubMed ID6312929
'Bovine viral diarrhea virus (BVDV) was concentrated and purified by a combination of ultrafiltration, hydroextraction using polyethylene glycol and affinity chromatography. A lectin from Crotalaria juncea that has an affinity for galactose was used in the affinity chromatography. Virions of BVDV with classic envelopes were observed by electron microscopy. Four ... More
Accessibility of the carbohydrate moiety of membrane-boound rhodopsin to enzymatic and chemical modification.
AuthorsShaper JH, Stryer L
JournalJ Supramol Struct
PubMed ID592816
'Galactose was specifically inserted into the carbohydrate moiety of rhodopsin by incubating retinal disk membranes with UDP-galactose: N-acetylglucosamine galactosyltransferase. The stoichiometry of labeling ranged from 1.2 to 1.8 (average = 1.5) residues of galactose per molecule of rhodopsin, indicating that some or all of the oligosaccharide chains of membrane-bound rhodopsin ... More
Characterization of the major proteins of bovine seminal fluid by two-dimensional polyacrylamide gel electrophoresis.
AuthorsDesnoyers L, Thérien I, Manjunath P
JournalMol Reprod Dev
PubMed ID8011327
'Recently, we demonstrated that the major proteins from bovine seminal plasma BSP-A1, -A2, -A3 and -30-kDa (collectively called BSP proteins) specifically interact with choline phospholipids. These proteins coat the surface of the spermatozoa after ejaculation and are believed to play an important role in membrane modifications occurring during capacitation. In ... More
Binding site of a dextran-specific homogeneous IgM: thermodynamic and spectroscopic mapping by dansylated oligosaccharides.
AuthorsSchepers G, Blatt Y, Himmelspach K, Pecht I
JournalBiochemistry
PubMed ID667022
'The hapten binding properties of the homogeneous mouse IgM secreted by MOPC-104E were investigated. Hapten-association constants were determined either by equilibrium and displacement equilibrium dialysis or by fluorometric titrations of the protein with the fluorescent derivatives of the haptens. For the latter type of measurements, several oligosaccharides were derivatized to ... More
A map of photolytic and tryptic cleavage sites on the beta heavy chain of dynein ATPase from sea urchin sperm flagella.
AuthorsMocz G, Tang WJ, Gibbons IR
JournalJ Cell Biol
PubMed ID2453517
'NH2-terminal analysis of the alpha and beta heavy chain polypeptides (Mr greater than 400,000) from the outer arm dynein of sea urchin sperm flagella, compared with that of the 230,000- and 200,000-Mr peptides formed upon photocleavage of dynein by irradiation at 365 nm in the presence of vanadate and ATP, ... More
Laser-induced fluorescence and UV detection of derivatized aldehydes in air samples using capillary electrophoresis.
AuthorsPereira EA, Carrilho E, Tavare MF
JournalJ Chromatogr A
PubMed ID12498272
'In this work, two capillary zone electrophoresis methodologies using UV absorption detection (214 nm) and laser-induced fluorescence detection (He/Cd laser, 325 nm excitation, 520 nm emission) of selected aldehydes (formaldehyde, acetaldehyde, propionaldehyde and acrolein) derivatized with dansylhydrazine (DNSH, 5-dimethylaminonaphthalene-1-sulfohydrazide) were proposed and validated. The aldehydes react with DNSH to form ... More
Direct determination of four sulfates and seven glucuronides of 17-oxosteroids in urine by fluorescence "high-performance" liquid chromatography.
AuthorsIwata J, Suga T
JournalClin Chem
PubMed ID2720972
'We describe a direct method for determining four sulfates and seven glucuronides of 17-oxosteroids (17OS) in urine without hydrolysis, by use of "high-performance" liquid chromatography (HPLC) with fluorometric detection. After pretreatment of urine samples with a Sep-Pak C18 cartridge, four 17OS sulfates and seven 17OS glucuronides in the pretreated urine ... More
Adsorption and covalent immobilization of human serum albumin (HSA) and gamma globulins (gamma G) onto poly(styrene/acrolein) latexes with pyrene, dansyl, and 2,4-dinitrophenyl labels.
AuthorsMiksa B, Slomkowski S
JournalJ Biomater Sci Polym Ed
PubMed ID7545003
'The poly(styrene/acrolein) latexes (P(SA)1 and P(SA)2), differing in poly(acrolein) content, were synthesized by the emulsifier-less emulsion-precipitation polymerization of styrene and acrolein. The fraction of poly(acrolein) in the surface layer was 0.35 and 0.50, for the P(SA)1 and P(SA)2 latex, respectively. Latexes were labelled with 2,4-dinitrophenylhydrazine (DNPH), dansylhdrazine (DAH), and 1-aminopyrene ... More
Interaction between rat alpha 1 fetoprotein and fluorescent derivatives of estrone in relation to the position and type of the fluorescent label.
AuthorsDesfosses B, Herve F, Moenner M, Urios P, Cittanova N, Dessen P
JournalJ Steroid Biochem
PubMed ID6200695
'A series of derivatives of estrone with fluorescent dyes (dansyl or coumarin) coupled at different positions on the steroid molecule, have been synthesized. These derivatives were tested for their quantum yields and their binding properties were determined with respect to rat alpha 1 fetoprotein. Derivatives at C-3 (estrone-3-hemisuccinate-dansyl-cadaverine and estrone-3-dansyl) ... More
Formation and separation by reversed-phase high-performance liquid chromatography of fluorescent and UV-absorbing bile salt derivatives.
AuthorsReid AD, Baker PR
JournalJ Chromatogr
PubMed ID6863432
'A method is described for the production of bile salt derivatives via the substituent hydroxyl groups on the steroid nucleus that permits derivatization of taurine and glycine conjugates. The method involves the oxidation of the hydroxyl groups at C-3 alpha or C-7 alpha on the nucleus by the action of ... More
Fluorescent labeling of the carbohydrate moieties of human chorionic gonadotropin and alpha 1-acid glycoprotein.
AuthorsIngham KC, Brew SA
JournalBiochim Biophys Acta
PubMed ID6271241
'A method for covalent attachment of a fluorescent molecule to the carbohydrate moieties of glycoproteins is described. The glycoproteins were oxidized with periodate under mild conditions selective for sialic acid (Van Lenten, L. and Ashwell, G. (1971) J. Biol. Chem. 246, 1889--1894). The resulting aldehydes were condensed with either dansylhydrazine, ... More
Determination of 2,5-hexandione by high-performance liquid chromatography after derivatization with dansylhydrazine.
AuthorsMaestri L, Ghittori S, Imbriani M, Capodaglio E
JournalJ Chromatogr B Biomed Appl
PubMed ID7952056
'A sensitive method for the determination of free and total urinary 2,5-hexandione (2,5-HD) using high-performance liquid chromatography with fluorescence detection was developed. After purification of urine with a disposable C18 cartridge, 2,5-HD was derivatized with dansylhydrazine; 1,3-diacetyl benzene (1,3-DAB) was added to the samples, as internal standard, prior to extraction. ... More
Studies on factor VIII-related protein. III. Size distribution and carbohydrate content of human and bovine factor VIII.
AuthorsFurlan M, Perret BA, Beck EA
JournalBiochim Biophys Acta
PubMed ID534647
'Human and bovine factor VIII were isolated from cryoprecipitate of fresh frozen plasma by gel filtration on Sepharose CL-2B. The elution diagrams and SDS-agarose electrophoretic analysis of eluted fractions show no significant differences in the size-distribution of factor VIII aggregates between the two species. Agarose gels were stained for carbohydrate ... More
Dansyl hydrazine labeling of polysaccharide bodies in human brain.
AuthorsSparkman DR
JournalBiotech Histochem
PubMed ID7684266
'A method is described for selective fluorescent staining of polysaccharide bodies, such as those found in Alzheimer''s disease, Lafora''s disease and adult polyglucosan disease. Formalin fixed, paraffin embedded sections of human autopsied brain tissue were stained with the fluorescent compound dansyl hydrazine. It specifically stained polysaccharide bodies in tissue sections ... More
Charge asymmetry of the purple membrane measured by uranyl quenching of dansyl fluorescence.
AuthorsRenthal R, Cha CH
JournalBiophys J
PubMed ID6733237
'Purple membrane was covalently labeled with 5-(dimethylamino) naphthalene-1-sulfonyl hydrazine (dansyl hydrazine) by carbodiimide coupling to the cytoplasmic surface (carboxyl-terminal tail: 0.7 mol/mol bacteriorhodopsin) or by periodate oxidation and dimethylaminoborane reduction at the extracellular surface (glycolipids: 1 mol/mol). In 2 mM acetate buffer, pH 5.6, micromolar concentrations of UO2 +(2) were ... More
Determination of 17-oxosteroid sulphates in serum by ion-pair extraction, prelabelling with dansylhydrazine and high-performance liquid chromatography with fluorescence detection.
AuthorsIwata J, Suga T
JournalJ Chromatogr
PubMed ID2777951
'A new high-performance liquid chromatographic (HPLC) method with fluorescence detection is described for the direct determination of four serum 17-oxosteroid sulphates. Each serum sample was deproteinated with methanol, the methanol was evaporated and 17-oxosteroid sulphates in the residue were extracted with benzene as ion pairs in the presence of tetrapentylammonium ... More
Multiresidue method for determination of eight neutral beta-lactam penicillins in milk by fluorescence-liquid chromatography.
AuthorsMunns RK, Shimoda W, Roybal JE, Vieira C
JournalJ Assoc Off Anal Chem
PubMed ID4055646
'A method of determining total penicillins begins with an enzymatic hydrolysis of the beta-lactam ring to form their respective penicilloate product. Acetonitrile precipitates much of the casein and protein, which are then separated from the liquid by centrifugation. The lipids are removed from the aqueous fraction with methylene chloride. Mercuric ... More
Determination of progesterone and 17-hydroxyprogesterone by high performance liquid chromatography after pre-column derivatization with 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionohydra zide.
AuthorsKatayama M, Nakane R, Matsuda Y, Kaneko S, Hara I, Sato H
JournalAnalyst
PubMed ID10396811
'Progesterone, 17-hydroxyprogesterone and four other 3-keto steroids were determined by high performance liquid chromatography with fluorescence detection. Each steroid was derivatized with 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene++ +-3- propionohydrazide (BODIPY FL hydrazide) and separated on a Wakosil 5C4 column with acetonitrile-water (7 + 3) as mobile phase. The limits of detection of progesterone, 17-hydroxyprogesterone, ... More
A reliable and quick radial thin layer chromatographic method for the diagnosis of mono- and disaccharide disorders.
AuthorsBiou D, Dutot G, Pays M
JournalJ Clin Chem Clin Biochem
PubMed ID6619737
'The authors propose a radial thin layer chromatographic method for the separation of mono- and disaccharides, which shows better resolution, and is more sensitive (0.25 microgram for glucose) and less time-consuming (20 min) than the linear technique. Moreover, the procedures used for the detection of sugars and their dansyl-derivatives (staining ... More
Stopped-flow cryoenzymology: detection of catalytic intermediates not observable at ambient temperatures.
AuthorsVan Wart HE, Lin SH
JournalProc Natl Acad Sci U S A
PubMed ID6584868
'Stopped-flow cryoenzymology has been used to study the reaction of porcine kidney cytosol leucine aminopeptidase [alpha-aminoacyl-peptide hydrolase(cytosol), EC 3.4.11.1] with L-leucylglycyldansyl hydrazide in 50% (vol/vol) methanol buffer over the -40 to 23 degrees C temperature range. Resonance energy transfer between tryptophan residues of the enzyme E and the dansyl group ... More
[Fluorodensitometric determination of cytostatic acting Michael-adducts of alpha, beta-unsaturated aldehydes in biologic material]
AuthorsTillian HM, Gübitz G, Korsatko W, Wintersteiger R
JournalArzneimittelforschung
PubMed ID4039578
'Alpha, beta-unsaturated aldehydes produce a selective cytostatic effect on tumor cells. By the formation of Michael adducts with cysteine, toxicity can be greatly reduced without impairing cytostatic effectiveness. To further enhance the selectivity of the toxic effect, it is necessary to be able to follow the agent''s kinetics in the ... More
The histochemical application of dansylhydrazine as a fluorescent labeling reagent for sialic acid in cellular glycoconjugates.
AuthorsWeber P, Harrison FW, Hof L
JournalHistochemistry
PubMed ID55401
'A new method for the fluorescent staining of stalic acid-containing glycoconjugates in fixed tissues is described. The procedure uses mild periodate oxidation, followed by condensation with dansylhydrazine and reduction of the hydrazones to hydrazines. The specificity of the reaction for sialic acid is tested on model glycoconjugates. The procedure gives ... More
Covalent labelling of specific membrane carbohydrate residues with fluorescent probes.
AuthorsAbraham G, Low PS
JournalBiochim Biophys Acta
PubMed ID7370252
'Two new fluorescent labelling techniques are described: one specific for sialic acid residues and a second specific for galactose and some of its derivatives. Using either technique it is possible to label covalently the desired carbohydrate residue with any one of a large variety of fluorescent probes. When the sialic ... More
Determination of tetraprenylacetone in human plasma by high-performance liquid chromatography with fluorescence derivatization using dansylhydrazine.
AuthorsSeki T, Hashida N, Kanazawa T
JournalJ Chromatogr
PubMed ID3372634
A rapid and sensitive method for the determination of monosaccharides as their dansyl hydrazones by high-performance liquid chromatography.
AuthorsAlpenfels WF
JournalAnal Biochem
PubMed ID7283147
Sensitive detection of unsaturated disaccharides from chondroitin sulphates by thin-layer chromatography as their dansylhydrazine derivatives.
AuthorsShinomiya K, Hoshi Y, Imanari T
JournalJ Chromatogr
PubMed ID2500453
Determination of a novel potent immunosuppressant (FK-506) in rat serum and lymph by high-performance liquid chromatography with chemiluminescence detection.
AuthorsTakada K, Oh-Hashi M, Yoshikawa H, Muranishi S, Nishiyama M, Yoshida H, Hata T, Tanaka H
JournalJ Chromatogr
PubMed ID1703552
A sensitive fluorescent method for the detection of glycoproteins in polyacrylamide gels.
AuthorsEckhardt AE, Hayes CE, Goldstein IJ
JournalAnal Biochem
PubMed ID942095
Quantitative method for the detection of glucose in body fluids by high-performance liquid chromatography with fluorescence detection.
AuthorsFinden DA, Fysh RR, White PC
JournalJ Chromatogr
PubMed ID4044748
Determination of individual urinary 17-ketosteroids as the dansyl-hydrazones.
AuthorsApter C, Chayen R, Gould S, Harell A
JournalClin Chim Acta
PubMed ID4266006
Elastic tissue histochemistry.
AuthorsKeith DA, Paz MA, Gallop PM
JournalAdv Exp Med Biol
PubMed ID868657
Introduction of a fluorescent label into the carbohydrate moiety of glycoconjugates.
AuthorsWeber P, Hof L
JournalBiochem Biophys Res Commun
PubMed ID209788
The distance between the contact sites for DNP and menadione ligands in the combining region of myeloma proteins binding both haptens--I. Estimation of distance by fluorescent energy transfer in protein 460.
AuthorsManjula BN, Richards FF, Rosenstein RW
JournalImmunochemistry
PubMed ID1010601
Constraints on the flexibility of bacteriorhodopsin's carboxyl-terminal tail at the purple membrane surface.
AuthorsRenthal R, Dawson N, Tuley J, Horowitz P
JournalBiochemistry
PubMed ID6830763
Fibrin-stabilizing factor inhibitors. 3. Sulphonamides related to monodansylcadaverine.
AuthorsNilsson JL, Stenberg P, Ljunggren C, Eriksson O, Lundén R
JournalActa Pharm Suec
PubMed ID5159519
Staining of glycoproteins in polyacrylamide and agarose gels with fluorescent lectins.
AuthorsFurlan M, Perret BA, Beck EA
JournalAnal Biochem
PubMed ID91330
Dansyl hydrazine as a fluorimetric reagent for thin-layer chromatographic analysis of reducing sugars.
AuthorsAvigad G
JournalJ Chromatogr
PubMed ID893622
Reducing sugars, particularly aldoses, react readily with dansyl hydrazine. The fluorescent hydrazones produced can be separated by thin-layer chromatography and determined quantitatively by spectrofluorimetry after elution from the chromatograms. ... More
The fluorescent demonstration of tissue aldehydes with dansylhydrazine.
AuthorsHarrison FW, Weber P, Hof L
JournalHistochemistry
PubMed ID993075
Dansylhydrazine, previously introduced in a selective fluorescent cytochemical method for the demonstration of sialic acid residues of cellular glycoconjungates, may be broadly applied as a specific, covalently bonded fluorochrome of aldehyde residues, both those naturally occurring as in elastin or those generated through PAS or Feulgen-type procedures. ... More
High performance liquid chromatographic determination of malondialdehyde in vegetable oils.
AuthorsHirayama T, Yamada N, Nohara M, Fukui S
JournalJ Assoc Off Anal Chem
PubMed ID6860411
A simple high performance liquid chromatographic (HPLC) method has been developed for determining malondialdehyde (MDA) in vegetable oils. MDA was reacted with dansyl hydrazine in an acidic medium, and the product, 1-dansyl-pyrazole, was determined by HPLC, using a Zorbax sil column with mixed mobile phase of n-hexane-methylene chloride. MDA can ... More
Separation of dansyl hydrazine-derivatized oligosaccharides by liquid chromatography.
AuthorsHull SR, Turco SJ
JournalAnal Biochem
PubMed ID3993926
A sensitive method has been developed for the rapid separation of neutral oligosaccharides derivatized with dansyl hydrazine by liquid chromatography on silica gel modified with 1,4-diaminobutane. Malto-oligosaccharides from corn syrup in amounts ranging from 7 to 280 nmol and ovalbumin-derived oligosaccharides in amounts ranging from 0.25 to 250 nmol were ... More
Synthesis of a biologically active fluorescent derivative of GM1, a main Ginseng saponin metabolite formed by intestinal bacteria.
AuthorsHasegawa H, Suzuki R, Wakabayashi C, Murata J, Tezuka Y, Saiki I, Kadota S
JournalBiol Pharm Bull
PubMed ID9635509
A fluorescent derivative of GM1 [20-O-beta-D-glucopyranosyl-20(S)-protopanaxadiol], a main Ginseng saponin metabolite formed by intestinal bacteria, was obtained from the condensation of its trisnor-aldehyde derivative with dansyl hydrazine. The dansylated GM1 fluoresced strongly and showed almost the same properties as its parent compound in lipophilicity and biological activities, so this fluorescent ... More
[Group determination of 17-oxosteroids on thin-layer chromotography]
AuthorsTomsová Z, Juzová Z
JournalSb Lek
PubMed ID1754831
17-oxosteroids from human urine are measured by thin layer chromatography (Merck Kieselgel F-254 plates) with fluorometric detection at 310 nm (densitometer SHIMADZU CS-9000): 11-OH-etiocholanolone, 11-OH-androsterone + oxoetiocholanolone, oxoandrosterone, dehydroepiandrosterone and androsterone. Fluorescence of hydrazone derivatives after the reaction of steroids with dansylhydrazine represents the principle of the method. The recoveries ... More
Thin-layer chromatography of urinary 17-oxosteroids using dansylhydrazine as a prelabelling reagent.
AuthorsTomsová Z
JournalJ Chromatogr
PubMed ID1797855
This paper describes a modification of a high-performance liquid chromatographic method for measurement of 17-oxosteroids in biological fluids for use with thin-layer chromatography and fluorometric scanning detection. After extraction from urine samples with Separon-C18 microcolumns, free oxosteroids were labelled with dansylhydrazine in acetonitrile-acetic acid and chromatographed on silica gel F-254 ... More
Fluorometric analysis of urinary chondroitin sulfate isomers by HPLC using dansylhydrazine as a prelabeling reagent.
AuthorsShinomiya K, Yamanashi S, Imanari T
JournalBiomed Chromatogr
PubMed ID3148350
Chondroitin sulfate isomers (ChS isomers) were digested with chondroitinase ABC, and the unsaturated disaccharides produced were converted into fluorescent dansylhydrazine derivatives and analyzed by HPLC. The proposed method was applied to the determination of relative amounts of ChS isomers in normal human urine and analytical results showed that chondroitin-6-sulfate and ... More
A strong ethidium binding site in the acceptor stem of most or all transfer RNAs.
AuthorsWells BD, Cantor CR
JournalNucleic Acids Res
PubMed ID331262
E. coli unfractionated tRNA and tRNA phe both contain a single strong ethidium binding site. Singlet-singlet energy transfer has been used to measure the distance between this site and dansyl hydrazine covalently attached to the 3' end of the tRNAs. The distance obtained is between 33 and 40 A for ... More
Steroid analysis in single cells by capillary electrophoresis with collinear laser-induced fluorescence detection.
AuthorsMalek A, Khaledi MG
JournalAnal Biochem
PubMed ID10328764
Capillary electrophoresis with collinear laser-induced fluorescence detection was used for the analysis of steroids in single R2C cells. Progesterone secretion was monitored from cultured cells and subsequently detected in single cells. Mass detection limit of 10(-18) mol for dansylated steroids was achieved with the 325-nm line of a helium-cadmium laser. ... More
New biochemical separations using precolumn derivatization and microcolumn liquid chromatography.
AuthorsNovotny M, Karlsson KE, Konishi M, Alasandro M
JournalJ Chromatogr
PubMed ID6547449
Microcolumn liquid chromatography with slurry-packed capillary columns has been used to resolve mixtures of biologically important steroids and prostaglandins. In order to enhance detection sensitivity, the samples are first derivatized with a suitable chromophore-yielding agent. Hydroxy steroids were derivatized with either benzoyl chloride or 7-(chlorocarbonylmethoxy)-4-methylcoumarin, while Dns hydrazine was employed ... More
Analysis of chondroitin sulfate isomers in the periodontium of the monkey using high-performance liquid chromatography.
AuthorsOkazaki J, Kamada A, Gonda Y, Sakaki T
JournalJ Periodontal Res
PubMed ID1403577
Glycosaminoglycan (GAG) was extracted from monkey periodontium, consisting of gingiva, periodontal ligament, alveolar bone and cementum, and from dental pulp and dentin by digestion with Pronase E. Unsaturated disaccharide isomers formed by chondroitinase AC digestion from chondroitin sulfate were labeled with dansylhydrazine and analyzed by high-performance liquid chromatography. These tissues ... More
Determination of carbohydrates as their dansylhydrazine derivatives by capillary electrophoresis with laser-induced fluorescence detection.
AuthorsPerez SA, Colón LA
JournalElectrophoresis
PubMed ID8900942
A mixture of mono- and disaccharides was derivatized with dansylhydrazine in a relatively fast chemical reaction (15 min) which is selective toward aldehydes and ketones. The products of the derivatization reaction were then separated by capillary electrophoresis (CE) using laser-induced fluorescence (LIF) as the detection mode. The smallest amount of ... More
Determination of 17-oxosteroid glucuronides and sulfates in urine and serum by fluorescence high-performance liquid chromatography using dansyl hydrazine as a prelabeling reagent.
AuthorsKawasaki T, Maeda M, Tsuji A
JournalJ Chromatogr
PubMed ID7161363
A fluorescence high-performance liquid chromatographic method is described for the direct determination of conjugated 17-oxosteroids in biological fluids without hydrolysis. Conjugated 17-oxosteroids are extracted with Sep-Pak C18 cartridge, labeled with dansyl hydrazine in trichloroacetic acid--benzene solution and then separated by high-performance liquid chromatography on reversed-phase muBondapak C18 column using 0.01 ... More
Functionalized C-glycoside ketohydrazones: carbohydrate derivatives that retain the ring integrity of the terminal reducing sugar.
AuthorsPrice NP, Bowman MJ, Le Gall S, Berhow MA, Kendra DF, Lerouge P,
JournalAnal Chem
PubMed ID20192186
Glycosylation often mediates important biological processes through the interaction of carbohydrates with complementary proteins. Most chemical tools for the functional analysis of glycans are highly dependent upon various linkage chemistries that involve the reducing terminus of carbohydrates. However, because of ring opening, the structural integrity of the reducing sugar ring ... More
Fluorescence labeling and quantification of oxygen-containing functionalities on the surface of single-walled carbon nanotubes.
AuthorsDementev N, Feng X, Borguet E,
JournalLangmuir
PubMed ID19563231
Fluorescence labeling of surface species (FLOSS) was applied to identify and determine the concentration of oxygen-containing functionalities on single-walled carbon nanotubes (SWCNTs), subjected to two different purification processes (air/HCl and nitric acid treatments) and compared to as-received (nonpurified) SWCNTs. The fluorophores were selected for their ability to covalently bind, with ... More
Immobilized 3 alpha-hydroxysteroid dehydrogenase and dansyl hydrazine as a pre-labeling reagent for high-performance liquid chromatography with fluorescence detection of bile acids.
AuthorsKawasaki T, Maeda M, Tsuji A
JournalJ Chromatogr
PubMed ID6572627
A high-performance liquid chromatographic method with fluorescence detection is described for the determination of bile acids and their conjugates. After enzymatic conversion of bile acids to 3-oxo-bile acids using the immobilized 3 alpha-hydroxysteroid dehydrogenase reactor column, 3-oxo-bile acids were extracted with a Sep-Pak C18 cartridge, labelled with dansyl hydrazine and ... More
Determination of conjugated and unconjugated serum 3 alpha-OH bile acids by high-performance liquid chromatography.
AuthorsGoldsmith RF, Gruca M, O'Halloran MT, Earl JW, Gaskin K
JournalAnn Clin Biochem
PubMed ID7832575
A simple reverse-phase liquid chromatographic method has been developed for the quantitative measurement of 11 3 alpha-OH bile acids in paediatric serum samples. Bile acids are enzymatically reduced to the corresponding 3-keto compound and then derivatized with the fluorophore dansyl hydrazine. Preliminary fractionation of bile acids is not required. The ... More
The N-terminal residue of bovine rhodopsin is acetylmethionine.
AuthorsTsunasawa S, Narita K, Shichi H
JournalBiochim Biophys Acta
PubMed ID7407235
By affinity chromatography on a concanavalin A-Sepharose column and gel filtration on a Sephadex G-25 column, a glycopeptide of 16 amino acid residues has been separated from a tryptic digest of reduced and carboxymethylated bovine rhodopsin. The glycopeptide was treated with cyanogen bromide and products were subjected to high-voltage paper ... More
Fluorogenic reactions for biomedical chromatography.
AuthorsOhkura Y, Kai M, Nohta H
JournalJ Chromatogr B Biomed Appl
PubMed ID7820282
A number of fluorogenic reactions, which have been used for HPLC detection systems by means of pre- and/or postcolumn derivatization, are surveyed with respect to both sensitivity and selectivity for the determination of biomedically important substances. For the derivatization of the substances, two types of fluorogenic reactions, fluorescence-generating and fluorescence-tagging, ... More
Determination of 17-hydroxycorticosteroids in urine by fluorescence high-performance liquid chromatography using Dns-hydrazine as a pre-column labeling reagent.
AuthorsKawasaki T, Maeda M, Tsuji A
JournalJ Chromatogr
PubMed ID7142318
A method is described for the determination of urinary 17-hydroxycorticosteroids using fluorescence high-performance liquid chromatography. After enzymatic hydrolysis, 17-hydroxycorticosteroids were extracted using an Extrelut column and then labeled with Dns-hydrazine in hydrochloric acid-ethanol solution. The labeled steroids were chromatographed on a microparticulate silica gel column, the mobile phase was dichloromethane-ethanol-water ... More
Distance measurement by energy transfer: the 3' end of 16-S RNA and proteins S4 and S17 of the ribosome of Escherichia coli.
Escherichia coli ribosomal proteins S4 and S17 were specifically labelled at their thiol groups with the acetylaminoethyl-dansyl and/or bimane fluorophores. Each formed a complex with 16-S RNA and, when the other 30-S ribosomal proteins were added, a complete 30-S subunit with at least partial activity. If the 3' end of ... More
Ribosome binding by tRNAs with fluorescent labeled 3' termini.
AuthorsWells BD, Cantor CR
JournalNucleic Acids Res
PubMed ID6160468
Yeast and E. coli tRNAPhe samples were oxidized and labeled at the 3' end with dansyl hydrazine or fluorescein thiosemicarbazide. These tRNAs can bind to poly(U)-programmed E. coli 70S tight couple ribosomes in 25 mM magnesium at 8 degrees C. Two binding sites with binding constants of about 1 X ... More
Sensitive assay system for bile acids and steroids having hydroxyl groups utilizing high-performance liquid chromatography with peroxyoxalate chemiluminescence detection.
AuthorsHigashidate S, Hibi K, Senda M, Kanda S, Imai K
JournalJ Chromatogr
PubMed ID2283379
3 alpha- or 3 beta-hydroxysteroids, such as bile acids (free and glycine and taurine conjugates), 3 beta-hydoxy-5-cholenic acid, pregnanediol, 5-pregnene-3 beta, 20 beta-diol and 5-pregnene-3-beta,20 alpha-diol, were converted to 3-oxosteroids by enzymatic reaction using immobilized hydroxysteroid dehydrogenase, derivatized with dansylhydrazine to the corresponding dansyl hydrazones and purified by gel permeation ... More
Fluorescent hydrazides for the high-performance liquid chromatographic determination of biological carbonyls.
AuthorsAnderson JM
JournalAnal Biochem
PubMed ID2937341
Methods for the determination of carbonyl compounds of biological origin by high-performance liquid chromatography were improved by the use of new fluorescent derivatizing agents. Eight fluorescent hydrazides were either synthesized or obtained commercially and compared to dansyl hydrazine (1-dimethylaminonaphthalene-5-sulfonylohydrazide). Four of the compounds yielded carbonyl hydrazones with a higher relative ... More
Determination of 17-oxosteroids in serum and urine by fluorescence high-performance liquid chromatography using dansyl hydrazine as a pre-labeling reagent.
AuthorsKawasaki T, Maeda M, Tsuji A
JournalJ Chromatogr
PubMed ID7320136
A fluorescence high-performance liquid chromatographic method is described for the determination of 17-oxosteroids in biological fluids. 17-Oxosteroids in urine samples are extracted with dichloromethane after enzymatic hydrolysis (beta-glucuronidase-sulfatase), and dehydroepiandrosterone sulfate in serum samples is solvolysed with sulfuric acid in ethyl acetate. 17-Oxosteroids are labeled with dansyl hydrazine in trichloroacetic ... More
Optimization of erythrocyte membrane glycoprotein fluorescent labeling with dansylhydrazine after polyacrylamide gel electrophoresis.
AuthorsEstep TN, Miller TJ
JournalAnal Biochem
PubMed ID3766952
An improved procedure for the labeling of glycoproteins with dansylhydrazine subsequent to electrophoresis in polyacrylamide gels is reported. This procedure is derived from the work of Eckhardt et al. (1976, Anal. Biochem. 73, 192-197) and Weber and Hof (1975, Biochem. Biophys. Res. Commun. 65, 1298-1302) who showed that dansylhydrazine may ... More
Multiphoton fluorescence excitation: new spectral windows for biological nonlinear microscopy.
AuthorsXu C, Zipfel W, Shear JB, Williams RM, Webb WW
JournalProc Natl Acad Sci U S A
PubMed ID8855254
Intrinsic, three-dimensionally resolved, microscopic imaging of dynamical structures and biochemical processes in living preparations has been realized by nonlinear laser scanning fluorescence microscopy. The search for useful two-photon and three-photon excitation spectra, motivated by the emergence of nonlinear microscopy as a powerful biophysical instrument, has now discovered a virtual artist's ... More
Fluorescent labeling of bacteriorhodopsin: implications for helix connections.
AuthorsRenthal R, Cothran M, Dawson N, Harris GJ
JournalBiochim Biophys Acta
PubMed ID3101736
Purple membrane from Halobacterium halobium was reacted with dansyl (5-dimethylamino-1-naphthalenyl fluorescent labels that have specificity for different protein side chains of bacteriorhodopsin. Dansyl chloride was found to react primarily with Lys-41. Dansyl hydrazine was coupled, with water-soluble carbodiimide, to Glu-74 and/or Asp-85, which was the major modified site after papain-cleavage ... More
Barley cultivar discrimination: I. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and glycoprotein blotting.
AuthorsWeiss W, Postel W, Görg A
JournalElectrophoresis
PubMed ID1718734
Two different methods of detecting electroblotted glycoproteins after sodium dodecyl sulfate-polyacrylamide gel electrophoresis of Tris-buffer soluble barley seed proteins were examined for their applicability for barley cultivar discrimination. These are the highly specific, lectin-based concanavalin A/peroxidase method and the more general periodate/danyslhydrazine method. The results of the periodate/dansylhydrazine method enabled ... More
[Recent development of determination methods for adrenocortical hormones and catecholamines]
AuthorsNozaki O, Ohba Y
JournalRinsho Byori
PubMed ID2262972
Newly developed resins and high-performance liquid chromatography (HPLC) with column switching technique have enabled on-line solid-phase extraction, purification and derivatization for highly sensitive determination in recently developed determination methods for serum cortisol, urinary 17-ketosteroids (17-KS), urinary conjugated 17-KS fractions and urinary free catecholamines (CA). These methods are (1) determination of ... More
Measurement of neutral sugars in glycoproteins as dansyl derivatives by automated high-performance liquid chromatography.
AuthorsEggert FM, Jones M
JournalJ Chromatogr
PubMed ID4066837
Automated high-performance liquid chromatography was used to analyse dansylhydrazine derivatives of neutral sugars in unfractionated acid hydrolysates of four well-characterized glycoproteins: fetuin, ovalbumin, alpha-1-acid glycoprotein and bovine submaxillary mucin. After a simple single-step derivatization at 65 degrees C the sugar derivatives in protein hydrolysates chromatographed as single peaks on reversed-phase ... More
Determination of the chondroitin sulfate disaccharides in dog and horse plasma by HPLC using chondroitinase digestion, precolumn derivatization, and fluorescence detection.
AuthorsDu J, Eddington N
JournalAnal Biochem
PubMed ID12123663
A sensitive and selective HPLC method for the determination of the disaccharides of chondroitin sulfate in horse and dog plasma was validated. Chondroitin sulfate is degraded by chondroitinase ABC to three primary unsaturated disaccharides, (1) 2-acetamido-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-D-galactose, (2) 2-acetamido-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-4-O-sulfo-D-galactose, and (3) 2-acetamido-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-6-O-sulfo-D-galactose, when treated with chondroitinase. Plasma samples ... More
Isolation and characterization of gastric microsomal glycoproteins. Evidence for a glycosylated beta-subunit of the H+/K(+)-ATPase.
AuthorsOkamoto CT, Karpilow JM, Smolka A, Forte JG
JournalBiochim Biophys Acta
PubMed ID1690026
Detergent-solubilization of hog gastric microsomal membrane proteins followed by affinity chromatography using wheat germ agglutinin or Ricinus communis I agglutinin resulted in the isolation of five glycoproteins with the apparent molecular masses on sodium dodecyl sulfate polyacrylamide gels of (in kDa): 60-80 (two glycoproteins sharing this molecular mass); 125-150; and ... More
Perfluorosulfonated ionomer-modified polyethylene. A material for simultaneous solid-phase enrichment and enhanced precolumn dansylation of C-21 ketosteroids in human serum.
AuthorsAppelblad P, Ahmed A, Pontén E, Bäckström T, Irgum K
JournalAnal Chem
PubMed ID11510837
A new derivatization procedure has been developed where solid-phase catalysis is utilized to facilitate the formation of hydrazones in precolumn labeling of keto-containing compounds. This procedure has been implemented on a solid-phase enrichment and enhanced derivatization (SPEED) device, prepared from porous polyethylene that has been coated with Nafion and dansylhydrazine. ... More