Imaging farnesyl protein transferase using a topologically activated probe.
AuthorsPham W, Pantazopoulos P, Moore A,
JournalJ Am Chem Soc
PubMed ID16953595
'We report on the development and application of a "smart" activatable peptide-based probe for detection of Ras-related farnesyl protein transferase (FPT). Upon farnesylation by FPT, the probe was brought close to a hydrophobic milieu and as a consequence emitted fluorescent light that could be detected by several media, such as ... More
Peptide-based fluorescent sensors of protein kinase activity: design and applications.
AuthorsSharma V, Wang Q, Lawrence DS,
JournalBiochim Biophys Acta
PubMed ID17881302
Protein kinases control the flow of information through cell-signaling pathways. A detailed analysis of their behavior enhances our ability to understand normal cellular states and to devise therapeutic interventions for diseases. The design and application of ... More