Fretting about FRET: correlation between kappa and R.
AuthorsVanBeek DB, Zwier MC, Shorb JM, Krueger BP
JournalBiophys J
PubMed ID17384068
'Molecular dynamics simulations were used to examine the structural dynamics of two fluorescent probes attached to a typical protein, hen egg-white lysozyme (HEWL). The donor probe (D) was attached via a succinimide group, consistent with the commonly-used maleimide conjugation chemistry, and the acceptor probe (A) was bound into the protein ... More
Nanodiscs unravel the interaction between the SecYEG channel and its cytosolic partner SecA.
AuthorsAlami M, Dalal K, Lelj-Garolla B, Sligar SG, Duong F
JournalEMBO J
PubMed ID17396152
The translocon is a membrane-embedded protein assembly that catalyzes protein movement across membranes. The core translocon, the SecYEG complex, forms oligomers, but the protein-conducting channel is at the center of the monomer. Defining the properties of the SecYEG protomer is thus crucial to understand the underlying function of oligomerization. We ... More
Fluorigenic substrates for the protease activities of botulinum neurotoxins, serotypes A, B, and F.
AuthorsSchmidt JJ, Stafford RG
JournalAppl Environ Microbiol
PubMed ID12514008
The seven botulinum neurotoxins (BoNTs) are zinc metalloproteases that cleave neuronal proteins involved in neurotransmitter release and are among the most toxic natural products known. High-throughput BoNT assays are needed for use in antibotulinum drug discovery and to characterize BoNT protease activities. Compared to other proteases, BoNTs exhibit unusually stringent ... More