Photoreactive (caged) cyclic AMP analogs induce DNA synthesis in mammary epithelial cells.
AuthorsYuh IS, Sheffield LG
JournalCell Biol Int
PubMed ID7550068
'Dimethoxy-2-nitrobenzyl adenosine-3'',5'' cyclic monophosphate (DMNB) is a metabolically active, photolabile cyclic AMP analog that yields free cyclic AMP upon UV hydrolysis. The analog is useful in that it permits short term, transient elevations of intracellular cyclic AMP. Addition of DMNB (1-10 microM) to mouse mammary epithelial cells, followed by UV ... More
A role for second messengers in the control of activation-associated modification of the surface of Trichinella spiralis infective larvae.
AuthorsModha J, Kusel JR, Kennedy MW
JournalMol Biochem Parasitol
PubMed ID8538685
'The involvement of second messengers in the control of activation-induced changes to the surface of Trichinella spiralis infective larvae was investigated using membrane-permeant photo-activatable ''caged'' compounds to alter intracellular levels of inositol trisphosphate (IP3), calcium ions (Ca2+) and cyclic AMP (cAMP). Activation of larvae by incubation in culture medium containing ... More
Properties and uses of photoreactive caged compounds.
AuthorsMcCray JA, Trentham DR
JournalAnnu Rev Biophys Biophys Chem
PubMed ID2660825
Modulation of Ca currents in isolated frog atrial cells studied with photosensitive probes. Regulation by cAMP and Ca2+: a common pathway?
'We have studied the regulation of cardiac Ca current by intracellular cyclic AMP (cAMP) and Ca2+, using photosensitive, caged compounds and the whole-cell, patch-clamp technique in isolated frog atrial cells. Although both low voltage activated (LVA) and high voltage activated (HVA) Ca channels were found to be present in these ... More
New photoactivatable cyclic nucleotides produce intracellular jumps in cyclic AMP and cyclic GMP concentrations.
AuthorsNerbonne JM, Richard S, Nargeot J, Lester HA
JournalNature
PubMed ID6330568
'The cyclic nucleotides cyclic AMP and cyclic GMP are important intracellular messengers mediating the responses to neurotransmitters and neurohormones and regulating cellular function over a wide range of time scales. Despite the widespread acceptance of this second messenger mechanism in many systems, much remains unknown about their mechanism of action, ... More
Light-flash physiology with synthetic photosensitive compounds.
AuthorsGurney AM, Lester HA
JournalPhysiol Rev
PubMed ID3031713
Caged compounds: photorelease technology for control of cellular chemistry and physiology.
AuthorsEllis-Davies GC
JournalNat Methods
PubMed ID17664946
Caged compounds are light-sensitive probes that functionally encapsulate biomolecules in an inactive form. Irradiation liberates the trapped molecule, permitting targeted perturbation of a biological process. Uncaging technology and fluorescence microscopy are 'optically orthogonal': the former allows control, and the latter, observation of cellular function. Used in conjunction with other technologies ... More
Illuminating the chemistry of life: design, synthesis, and applications of
AuthorsLee HM, Larson DR, Lawrence DS,
JournalACS Chem Biol
PubMed ID19298086
Biological systems are characterized by a level of spatial and temporal organization that often lies beyond the grasp of present day methods. Light-modulated bioreagents, including analogs of low molecular weight compounds, peptides, proteins, and nucleic acids, represent a compelling strategy to probe, perturb, or sample biological phenomena with the requisite ... More
Systems analysis of PKA-mediated phosphorylation gradients in live cardiac myocytes.
AuthorsSaucerman JJ, Zhang J, Martin JC, Peng LX, Stenbit AE, Tsien RY, McCulloch AD
JournalProc Natl Acad Sci U S A
PubMed ID16905651
Compartmentation and dynamics of cAMP and PKA signaling are important determinants of specificity among cAMP's myriad cellular roles. Both cardiac inotropy and the progression of heart disease are affected by spatiotemporal variations in cAMP/PKA signaling, yet the dynamic patterns of PKA-mediated phosphorylation that influence differential responses to agonists have not ... More
Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering.
Authors Zhang J; Ma Y; Taylor S S; Tsien R Y;
JournalProc Natl Acad Sci U S A
PubMed ID11752448
The complexity and specificity of many forms of signal transduction are widely suspected to require spatial microcompartmentation of protein kinase and phosphatase activities, yet current relevant imaging methods such as phosphorylation-specific antibodies or fluorescent peptide substrates require fixation or microinjection and lack temporal or spatial resolution. We present a genetically ... More
Inhibition of inositol 1,4,5-trisphosphate-induced Ca2+ release by cAMP-dependent protein kinase in a living cell.
AuthorsTertyshnikova S, Fein A
JournalProc Natl Acad Sci U S A
PubMed ID9465064
Interaction of intracellular free calcium ([Ca2+]i) and cAMP signaling mechanisms was examined in intact single megakaryocytes by using a combination of single-cell fluorescence microscopy to measure [Ca2+]i and flash photolysis of caged Ca2+, inositol 1,4, 5-trisphosphate (IP3), or cAMP to elevate rapidly the concentration of these compounds inside the cell. ... More
Mercury-arc photolysis: a method for examining second messenger regulation of endothelial cell monolayer integrity.
AuthorsPatton WF, Alexander JS, Dodge AB, Patton RJ, Hechtman HB, Shepro D
JournalAnal Biochem
PubMed ID1653549
Cell-cell apposition in bovine pulmonary endothelial cell monolayers was modulated by inducing transient increases in intracellular adenosine 3':5'-cyclic monophosphate (cAMP) and 1,4,5-inositol triphosphate (IP3). This was accomplished by mercury-arc flash photolysis of o-nitrobenzyl derivatives of the second messengers (caged compounds). Second messenger release by the mercury-arc lamp was determined by ... More
Direct cAMP signaling through G-protein-coupled receptors mediates growth cone attraction induced by pituitary adenylate cyclase-activating polypeptide.
AuthorsGuirland C, Buck KB, Gibney JA, DiCicco-Bloom E, Zheng JQ
JournalJ Neurosci
PubMed ID12657686
Developing axons are guided to their appropriate targets by environmental cues through the activation of specific receptors and intracellular signaling pathways. Here we report that gradients of pituitary adenylate cyclase-activating polypeptide (PACAP), a neuropeptide widely expressed in the developing nervous system, induce marked attraction of Xenopus growth cones in vitro. ... More
Human lymphocytes transcribe the cystic fibrosis transmembrane conductance regulator gene and exhibit CF-defective cAMP-regulated chloride current.
Cystic fibrosis (CF) is the most common lethal genetic disease among Caucasians, primarily affecting epithelial tissues of the lung and gut. Mutations in a single gene, the cystic fibrosis transmembrane conductance regulator (CFTR), are responsible for this disease. Whether a physiological defect exists in the immune system of CF patients ... More