Nerve growth factor signaling regulates motility and docking of axonal mitochondria.
AuthorsChada SR, Hollenbeck PJ
JournalCurr Biol
PubMed ID15268858
'Axonal transport is thought to distribute mitochondria to regions of the neuron where their functions are required. In cultured neurons, mitochondrial transport responds to growth cone activity, and this involves both a transition between motile and stationary states of mitochondria and modulation of their anterograde transport activity. Although the exact ... More
The movement of fluorescent endocytic tracers in Plasmodium falciparum infected erythrocytes.
AuthorsHaldar K, Uyetake L
JournalMol Biochem Parasitol
PubMed ID1371847
'The fluorescent lipophilic probe 1,1''-dihexadecyl-3-3''-3-3''- tetramethylindocarbocyanine (diIC16) inserted in the red cell surface, functioned as a non-exchangeable lipid marker which was not metabolised or toxic in plasmodial cultures. Invasion by Plasmodium falciparum resulted in the internalisation of the lipid, suggesting the uptake of red cell membrane components during parasite entry. ... More
Vital staining of the hearing organ: visualization of cellular structure with confocal microscopy.
AuthorsFlock A, Scarfone E, Ulfendahl M
JournalNeuroscience
PubMed ID9466411
'Cells inside the intact organ of Corti were labelled with fluorescent probes reflecting various aspects of structure and function. The dyes were introduced into the perilymphatic space by perfusion of the scala tympani of the temporal bone from the guinea-pig maintained in isolation. The dyes were able to diffuse through ... More
Carbocyanine dyes with long alkyl side-chains: broad spectrum inhibitors of mitochondrial electron transport chain activity.
AuthorsAnderson WM, Trgovcich-Zacok D
JournalBiochem Pharmacol
PubMed ID7763312
'Certain indocarbocyanine, thiacarbocyanine, and oxacarbocyanine dyes possessing short alkyl side-chains (one to five carbons) are potent inhibitors of mammalian mitochondrial NADH-ubiquinone reductase (EC 1.6.99.3) activity (Anderson et al., Biochem Pharmacol 41: 677-684, 1991; Anderson et al., Biochem Pharmacol 45: 691-696, 1993; Anderson et al., Biochem Pharmacol 45: 2115-2122, 1993), and ... More
Submillisecond optical reporting of membrane potential in situ using a neuronal tracer dye.
AuthorsBradley J, Luo R, Otis TS, DiGregorio DA,
JournalJ Neurosci
PubMed ID19625510
'A major goal in neuroscience is the development of optical reporters of membrane potential that are easy to use, have limited phototoxicity, and achieve the speed and sensitivity necessary for detection of individual action potentials in single neurons. Here we present a novel, two-component optical approach that attains these goals. ... More
Lateral diffusion of lipids in model and natural membranes.
AuthorsTocanne JF, Dupou-Cézanne L, Lopez A
JournalProg Lipid Res
PubMed ID8022844
Stable and robust polymer nanotubes stretched from polymersomes.
AuthorsReiner JE, Wells JM, Kishore RB, Pfefferkorn C, Helmerson K
JournalProc Natl Acad Sci U S A
PubMed ID16432242
We create long polymer nanotubes by directly pulling on the membrane of polymersomes using either optical tweezers or a micropipette. The polymersomes are composed of amphiphilic diblock copolymers, and the nanotubes formed have an aqueous core connected to the aqueous interior of the polymersome. We stabilize the pulled nanotubes by ... More
New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy.
AuthorsWeston SA, Parish CR
JournalJ Immunol Methods
PubMed ID2212694
16 fluorochromes were examined for their ability to label viable lymphocytes in vitro and yield fluorescence detectable by fluorescence microscopy and flow cytometry. Of these fluorochromes, four intracellular dyes were found to be suitable for in vivo migration studies. They were H33342, the well known DNA-binding dye which excites and ... More
Microtubule behavior during guidance of pioneer neuron growth cones in situ.
AuthorsSabry JH, O'Connor TP, Evans L, Toroian-Raymond A, Kirschner M, Bentley D
JournalJ Cell Biol
PubMed ID1918146
The growth of an axon toward its target results from the reorganization of the cytoskeleton in response to environmental guidance cues. Recently developed imaging technology makes it possible to address the effect of such cues on the neural cytoskeleton directly. Although high resolution studies can be carried out on neurons ... More
Endocytic sorting of lipid analogues differing solely in the chemistry of their hydrophobic tails.
AuthorsMukherjee S, Soe TT, Maxfield FR
JournalJ Cell Biol
PubMed ID10087269
To understand the mechanisms for endocytic sorting of lipids, we investigated the trafficking of three lipid-mimetic dialkylindocarbocyanine (DiI) derivatives, DiIC16(3) (1,1'-dihexadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), DiIC12(3) (1,1'- didodecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), and FAST DiI (1,1'-dilinoleyl-3,3,3', 3'-tetramethylindocarbocyanine perchlorate), in CHO cells by quantitative fluorescence microscopy. All three DiIs have the same head group, but differ in ... More