In vivo evidence for microvascular oxidative stress in spontaneously hypertensive rats. Hydroethidine microfluorography.
AuthorsSuzuki H, Swei A, Zweifach BW, Schmid-Schönbein GW
JournalHypertension
PubMed ID7737720
The factors that predispose to the accelerated organ injury that accompanies the hypertensive syndrome have remained speculative and without a firm experimental basis. Indirect evidence has suggested that a key feature may be related to an enhanced oxygen radical production. The purpose of this study was to refine and use ... More
Evaluation of a flow cytometric fluorescence quenching assay of phagocytosis of sensitized sheep erythrocytes by polymorphonuclear leukocytes.
AuthorsVan Amersfoort ES, Van Strijp JA
JournalCytometry
PubMed ID7875036
A number of reports have been published describing phagocytosis assays for flow cytometric analysis. In some of these, the fluorescence quenching technique has been used to discriminate between adherent and ingested particles. In this report, we have evaluated the efficacy of a quantitative fluorescence quenching technique with crystal violet and ... More
Superoxide reacts with hydroethidine but forms a fluorescent product that is distinctly different from ethidium: potential implications in intracellular fluorescence detection of superoxide.
AuthorsZhao H, Kalivendi S, Zhang H, Joseph J, Nithipatikom K, Vásquez-Vivar J, Kalyanaraman B
JournalFree Radic Biol Med
PubMed ID12757846
Hydroethidine (HE) or dihydroethidium (DHE), a redox-sensitive probe, has been widely used to detect intracellular superoxide anion. It is a common assumption that the reaction between superoxide and HE results in the formation of a two-electron oxidized product, ethidium (E+), which binds to DNA and leads to the enhancement of ... More
The human salivary peptide histatin 5 exerts its antifungal activity through the formation of reactive oxygen species.
AuthorsHelmerhorst EJ, Troxler RF, Oppenheim FG
JournalProc Natl Acad Sci U S A
PubMed ID11717389
Previous studies have shown that the human salivary antifungal peptide histatin 5 is taken up by Candida albicans cells and associates intracellularly with mitochondria. The purpose of the present study was to investigate the biological consequence of this specific subcellular targeting. Histatin 5 inhibited respiration of isolated C. albicans mitochondria ... More
Granzyme A induces caspase-independent mitochondrial damage, a required first step for apoptosis.
AuthorsMartinvalet D, Zhu P, Lieberman J
JournalImmunity
PubMed ID15780992
'Granzyme A (GzmA) triggers cell death with apoptotic features by targeting the endoplasmic reticulum-associated SET complex, which contains the GzmA-activated DNase NM23-H1, its inhibitor SET, and Ape1. The SET complex was postulated to translocate to the nucleus in response to oxidative stress and participate in its repair. Because mitochondrial damage ... More
Affinity modulation of platelet integrin alphaIIbbeta3 by beta3-endonexin, a selective binding partner of the beta3 integrin cytoplasmic tail.
AuthorsKashiwagi H, Schwartz MA, Eigenthaler M, Davis KA, Ginsberg MH, Shattil SJ
JournalJ Cell Biol
PubMed ID9182673
'Platelet agonists increase the affinity state of integrin alphaIIbbeta3, a prerequisite for fibrinogen binding and platelet aggregation. This process may be triggered by a regulatory molecule(s) that binds to the integrin cytoplasmic tails, causing a structural change in the receptor. beta3-Endonexin is a novel 111-amino acid protein that binds selectively ... More
Distinct mitochondrial retrograde signals control the G1-S cell cycle checkpoint.
AuthorsOwusu-Ansah E, Yavari A, Mandal S, Banerjee U,
JournalNat Genet
PubMed ID18246068
'During electron transport, the mitochondrion generates ATP and reactive oxygen species (ROS), a group of partially reduced and highly reactive metabolites of oxygen. In this in vivo genetic analysis in Drosophila melanogaster, we establish that disruption of complex I of the mitochondrial electron transport chain specifically retards the cell cycle ... More
Expression of mitochondrial Apo2.7 molecules and caspase-3 activation in human lymphocytes treated with the ribosome-inhibiting mistletoe lectins and the cell membrane permeabilizing viscotoxins.
AuthorsBüssing A, Vervecken W, Wagner M, Wagner B, Pfüller U, Schietzel M
JournalCytometry
PubMed ID10486525
'BACKGROUND: It is unclear whether expression of newly described mitochondrial Apo2.7 molecules (7A6 antigen) is specific for apoptosis or may also occur in necrosis. METHODS: We incubated human lymphocytes with the apoptosis-inducing mistletoe lectin (ML) I and the cell membrane-permeabilizing viscotoxins (VT), and measured cell death-associated changes by flow cytometry. ... More
AuthorsMakino A, Skelton MM, Zou AP, Roman RJ, Cowley AW
JournalHypertension
PubMed ID11882628
'The present study examined whether chronic increased oxidative stress within the medulla of the kidney lowers medullary blood flow and leads to hypertension. Optical fibers were implanted into the renal cortex and medulla of uninephrectomized Sprague-Dawley rats (Harlan Sprague-Dawley, Madison, Wis) for the daily measurement of blood flow to these ... More
Effects of oxidants and glutamate receptor activation on mitochondrial membrane potential in rat forebrain neurons.
AuthorsScanlon JM, Reynolds IJ
JournalJ Neurochem
PubMed ID9832137
'Both glutamate and reactive oxygen species have been implicated in excitotoxic neuronal injury, and mitochondria may play a key role in the mediation of this process. In this study, we examined whether glutamate-receptor stimulation and oxidative stress interact to affect the mitochondrial membrane potential (delta psi). We measured delta psi ... More
Oxidative stress and mitochondrial glutathione in human lymphocytes exposed to clinically relevant anesthetic drug concentrations.
AuthorsDelogu G, Antonucci A, Moretti S, Marandola M, Tellan G, Signore M, Famularo G
JournalJ Clin Anesth
PubMed ID15217658
'STUDY OBJECTIVE: To evaluate the potential of compounds commonly used in anesthesia practice to affect the intracellular oxidant-antioxidant homeostasis of peripheral blood lymphocytes at clinically relevant concentrations; and to study the changes in reactive oxygen species production and measure the mitochondrial glutathione content. DESIGN: Prospective, in vitro study. SETTING: Experimental ... More
Deficiency of glutathione peroxidase-1 sensitizes hyperhomocysteinemic mice to endothelial dysfunction.
AuthorsDayal S, Brown KL, Weydert CJ, Oberley LW, Arning E, Bottiglieri T, Faraci FM, Lentz SR
JournalArterioscler Thromb Vasc Biol
PubMed ID12482825
'OBJECTIVE: We tested the hypothesis that deficiency of cellular glutathione peroxidase (GPx-1) enhances susceptibility to endothelial dysfunction in mice with moderate hyperhomocysteinemia. METHODS AND RESULTS: Mice that were wild type (Gpx1+/+), heterozygous (Gpx1+/-), or homozygous (Gpx1-/-) for the mutated Gpx1 allele were fed a control diet or a high-methionine diet ... More
Single-cell measurement of superoxide anion and hydrogen peroxide production by human neutrophils with digital imaging fluorescence microscopy.
AuthorsSzucs S, Vámosi G, Póka R, Sárváry A, Bárdos H, Balázs M, Kappelmayer J, Tóth L, Szöllosi J, Adány R
JournalCytometry
PubMed ID9725555
'Besides flow cytometry, fluorescence microscopy combined with computerized image analysis offers an alternative tool for assessing phagocyte oxidant generation at the single-cell level. This technique provides an opportunity for the direct visualization of cells and simultaneous measurement of cellular fluorescence intensity. Thus, we developed a simple method for the quantitative ... More
Protective effect of transforming growth factor-beta 1 on beta-amyloid neurotoxicity in rat hippocampal neurons.
'Neurodegeneration associated with Alzheimer''s disease is believed to involve toxicity to beta-amyloid (A beta) and related peptides. Treatment of cultured rat hippocampal neurons with A beta 1-40 (1 microM) or the active fragment A beta 25-35 (1 microM) for 5 days led to a approximately 40-50% decrease in neuronal viability. ... More
ET-1 stimulates pulmonary arterial smooth muscle cell proliferation via induction of reactive oxygen species.
AuthorsWedgwood S, Dettman RW, Black SM
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID11597896
'Recent studies implicate reactive oxygen species (ROS) such as superoxide anions and H(2)O(2) in the proliferation of systemic vascular smooth muscle cells (SMCs). However, the role of ROS in SMC proliferation within the pulmonary circulation remains unclear. We investigated the effects of endothelin-1 (ET-1), a potential SMC mitogen, on ROS ... More
Acute lung injury using oleic acid in the laboratory rat: establishment of a working model and evidence against free radicals in the acute phase.
AuthorsMcGuigan RM, Mullenix P, Norlund LL, Ward D, Walts M, Azarow K
JournalCurr Surg
PubMed ID14972232
'OBJECTIVE: To determine the optimal model of acute respiratory distress syndrome (ARDS) using oleic acid in our laboratory and to measure the presence or absence of free radicals in this model. DESIGN: This protocol consisted of 2 phases. During the first phase, various conditions were tested, to include different doses ... More
Flow cytometric analysis of cell suspensions exposed to shock waves in the presence of the radical sensitive dye hydroethidine.
AuthorsEndl E, Steinbach P, Hofstädter F
JournalUltrasound Med Biol
PubMed ID7571150
'The occurrence of intracellularly and extracellularly generated free radicals during shock wave exposure on an experimental Siemens lithotripter was tested with the radical sensitive dyes hydroethidine and dichlorofluorescin (DCFH). DCFH, a nonfluorescent compound, is oxidised to dichlorfluorescein (DCF) by hydrogen peroxide in the presence of peroxidase. DCF green fluorescence intensity ... More
'Synaptically released Zn2+ can enter and cause injury to postsynaptic neurons. Microfluorimetric studies using the Zn2+-sensitive probe, Newport green, examined levels of [Zn2+]i attained in cultured cortical neurons on exposure to N-methyl-D-asparte, kainate, or high K+ (to activate voltage-sensitive Ca2+ channels) in the presence of 300 microM Zn2+. Indicating particularly ... More
The NF-kappa B cascade is important in Bcl-xL expression and for the anti-apoptotic effects of the CD28 receptor in primary human CD4+ lymphocytes.
AuthorsKhoshnan A, Tindell C, Laux I, Bae D, Bennett B, Nel AE
JournalJ Immunol
PubMed ID10925251
'We explored the role of the NF-kappa B pathway in the survival of primary human CD4+ T lymphocytes during CD28 costimulation. Transduction of proliferating CD4+ T cells with a tetracycline-regulated retrovirus encoding for a dominant-interfering, degradation-resistant I-kappaBalpha (inhibitor of kappa B alpha factor) mutant induced apoptosis. Using DNA arrays, we ... More
Induction of CDK inhibitors (p21(WAF1) and p27(Kip1)) and Bak in the beta-lapachone-induced apoptosis of human prostate cancer cells.
AuthorsDon MJ, Chang YH, Chen KK, Ho LK, Chau YP
JournalMol Pharmacol
PubMed ID11259623
'beta-Lapachone, a novel anti-neoplastic drug, induces various cancer cells to undergo apoptosis. In a previous report, we showed that beta-lapachone-induced apoptosis of HL-60 cells is mediated by oxidative stress. However, in the present study, we found that beta-lapachone-induced apoptosis of human prostate cancer (HPC) cells may be independent of oxidative ... More
Oxidative stress in the pathogenesis of thoracic aortic aneurysm: protective role of statin and angiotensin II type 1 receptor blocker.
AuthorsEjiri J, Inoue N, Tsukube T, Munezane T, Hino Y, Kobayashi S, Hirata K, Kawashima S, Imajoh-Ohmi S, Hayashi Y, Yokozaki H, Okita Y, Yokoyama M
JournalCardiovasc Res
PubMed ID14553839
'OBJECTIVE: The pathogenesis of thoracic aortic aneurysms (TAA) is still unclear. A recent investigation indicated that angiotensin II, a potent activator of NADH/NADPH oxidase, plays an important role in aneurysmal formation. We investigated the potential role of p22phox-based NADH/NADPH oxidase in the pathogenesis of TAA. METHODS: Human thoracic aneurysmal (n=40) ... More
Oscillatory shear stress stimulates endothelial production of O2- from p47phox-dependent NAD(P)H oxidases, leading to monocyte adhesion.
AuthorsHwang J, Saha A, Boo YC, Sorescu GP, McNally JS, Holland SM, Dikalov S, Giddens DP, Griendling KK, Harrison DG, Jo H
JournalJ Biol Chem
PubMed ID12958309
'Arterial regions exposed to oscillatory shear (OS) in branched arteries are lesion-prone sites of atherosclerosis, whereas those of laminar shear (LS) are relatively well protected. Here, we examined the hypothesis that OS and LS differentially regulate production of O2- from the endothelial NAD(P)H oxidase, which, in turn, is responsible for ... More
High glucose-induced tyrosine nitration in endothelial cells: role of eNOS uncoupling and aldose reductase activation.
'PURPOSE: Analyses in diabetic rats have shown that breakdown of the blood-retina barrier is associated with increased formation of peroxynitrite, nitric oxide, and lipid peroxidation. The permeability increase is blocked by treatments that also prevent the increases in reactive oxygen species, suggesting their causal role in vascular dysfunction. The purpose ... More
Hydroxyl radical formation is greater in striatal core than in penumbra in a rat model of ischemic stroke.
AuthorsLiu S, Liu M, Peterson S, Miyake M, Vallyathan V, Liu KJ
JournalJ Neurosci Res
PubMed ID12605415
'Although hydroxyl radical ((*)OH) formation has been implicated in the pathophysiological changes of ischemic stroke, (*)OH production in the core and penumbra regions is not clear. It is extremely important to distinguish penumbra from ischemic core in focal cerebral ischemia studies, because the penumbra contains viable tissue, which can be ... More
Increased renal medullary H2O2 leads to hypertension.
AuthorsMakino A, Skelton MM, Zou AP, Cowley AW
JournalHypertension
PubMed ID12782642
'We have recently reported that exaggerated oxidative stress in the renal medulla due to superoxide dismutase inhibition resulted in a reduction of renal medullary blood flow and sustained hypertension. The present study tested the hypothesis that selective scavenging of O2*- in the renal medulla would prevent hypertension associated with this ... More
Quercetin modifies reactive oxygen levels but exerts only partial protection against oxidative stress within HL-60 cells.
AuthorsBestwick CS, Milne L
JournalBiochim Biophys Acta
PubMed ID11514098
'Quercetin may contribute to the protection afforded by fruit- and vegetable-rich diets against diseases for which excess production of reactive oxygen species (ROS) has been implicated as a causal or contributory factor. We examine the effect of short term (90 min) quercetin (1-100 microM) exposure on the progress of menadione ... More
Relationship between the intracellular reactive oxygen species and the induction of oxidative DNA damage in human neutrophil-like cells.
AuthorsTakeuchi T, Nakajima M, Morimoto K
JournalCarcinogenesis
PubMed ID8761407
'To clarify the mechanisms of intracellular induction of oxidative DNA damage, we have investigated the concentrations of intracellular reactive oxygen species and the amounts of 8-hydroxydeoxyguanosine (8OHdG), a mutagenic oxidative DNA damage, in human neutrophil-like cells, dimethylsulfoxide-differentiated HL60 (DMSO-HL60). We determined intracellular concentrations of hydrogen peroxide and superoxide by flow ... More
Intra- and extracellular measurement of reactive oxygen species produced during heat stress in diaphragm muscle.
AuthorsZuo L, Christofi FL, Wright VP, Liu CY, Merola AJ, Berliner LJ, Clanton TL
JournalAm J Physiol Cell Physiol
PubMed ID11003586
'Skeletal muscles are exposed to increased temperatures during intense exercise, particularly in high environmental temperatures. We hypothesized that heat may directly stimulate the reactive oxygen species (ROS) formation in diaphragm (one kind of skeletal muscle) and thus potentially play a role in contractile and metabolic activity. Laser scan confocal microscopy ... More
'Mild Uncoupling' does not decrease mitochondrial superoxide levels in cultured cerebellar granule neurons but decreases spare respiratory capacity and increases toxicity to glutamate and oxidative stress.
AuthorsJohnson-Cadwell LI, Jekabsons MB, Wang A, Polster BM, Nicholls DG
JournalJ Neurochem
PubMed ID17437552
'Cultured rat cerebellar granule neurons were incubated with low nanomolar concentrations of the protonophore carbonylcyanide-p-trifluoromethoxyphenyl hydrazone (FCCP) to test the hypothesis that ''mild uncoupling'' could be neuroprotective by decreasing oxidative stress. To quantify the uncoupling, respiration and mitochondrial membrane potential (Deltapsi(m)) were determined in parallel as a function of FCCP ... More
Attenuation of acute lung injury and oxygen radical production by the 21-aminosteroid, U-78518F.
AuthorsTanigaki T, Suzuki Y, Heimer D, Sussman HH, Ross WG, Raffin TA
JournalJ Appl Physiol
PubMed ID8335543
'Oxygen radicals play an important role in the mechanism of acute lung injury. The 21-aminosteroid lazaroid, U-78518F, is a potent antioxidant. We examined the effect of intravenous U-78518F on acute lung injury in septic guinea pigs over 8 h. The experimental groups (n = 6) were 1) saline control, 2) ... More
Mechanism of preconditioning by isoflurane in rabbits: a direct role for reactive oxygen species.
AuthorsTanaka K, Weihrauch D, Kehl F, Ludwig LM, LaDisa JF, Kersten JR, Pagel PS, Warltier DC
JournalAnesthesiology
PubMed ID12459675
'BACKGROUND: Reactive oxygen species (ROS) contribute to myocardial protection during ischemic preconditioning, but the role of the ROS in protection against ischemic injury produced by volatile anesthetics has only recently been explored. We tested the hypothesis that ROS mediate isoflurane-induced preconditioning in vivo. METHODS: Pentobarbital-anesthetized rabbits were instrumented for measurement ... More
Effect of extracellular Mg(2+) on ROS and Ca(2+) accumulation during reoxygenation of rat cardiomyocytes.
AuthorsSharikabad MN, Ostbye KM, Lyberg T, Brørs O
JournalAm J Physiol Heart Circ Physiol
PubMed ID11123250
'The effects of Mg(2+) on reactive oxygen species (ROS) and cell Ca(2+) during reoxygenation of hypoxic rat cardiomyocytes were studied. Oxidation of 2'',7''-dichlorodihydrofluorescein (DCDHF) to dichlorofluorescein (DCF) and of dihydroethidium (DHE) to ethidium (ETH) within cells were used as markers for intracellular ROS levels and were determined by flow cytometry. ... More
[Evaluation of free radical production by conjunctival impression cytology of patients treated with long-term antiglaucoma drugs or of contact lens wearers]
AuthorsDebbasch C, Pisella PJ, Rat P, Warnet JM, Baudouin C
JournalJ Fr Ophtalmol
PubMed ID10740049
'PURPOSE: To investigate free radical production in impression cytology specimens using microplate cold light cytofluorimetry. METHODS: 60 impression cytology specimens (IC) were harvested in 30 patients aged from 20 to 90 years. IC were taken in 10 healthy subjects, 10 glaucoma patients receiving longterm treatment for glaucoma and 10 contact ... More
Fluorescent vital stains for complementary labelling of protoplasts from Trichoderma spp.
AuthorsHarman GE, Stasz TE
JournalStain Technol
PubMed ID2464211
'In this study several fluorescent vital stains were evaluated for their ability to provide complementary vital staining of protoplasts of Trichoderma spp. for selection of heterokaryons following protoplast fusion. Tetramethyl rhodamine isothiocyanate and fluorescein isothiocyanate were rejected because they stained only a small proportion of protoplasts. Fluorescein diacetate stained all ... More
Role of Oxidative Stress in Amiodarone-induced Toxicity.
AuthorsSarma JS, Pei H, Venkataraman K
JournalJ Cardiovasc Pharmacol Ther
PubMed ID10684442
'BACKGROUND: The clinical usefulness of amiodarone for the treatment of cardiac arrhythmias is limited by multiorgan toxicity, especially pulmonary and hepatic. There are conflicting reports in the literature regarding the role of free radicals in the initiation of amiodarone-induced toxicity. We evaluated the possible oxidative stress in a chronic model ... More
Mitochondrial adenosine triphosphate-regulated potassium channel opening acts as a trigger for isoflurane-induced preconditioning by generating reactive oxygen species.
AuthorsTanaka K, Weihrauch D, Ludwig LM, Kersten JR, Pagel PS, Warltier DC
JournalAnesthesiology
PubMed ID12657856
'BACKGROUND: Whether the opening of mitochondrial adenosine triphosphate-regulated potassium (K(ATP)) channels is a trigger or an end effector of anesthetic-induced preconditioning is unknown. We tested the hypothesis that the opening of mitochondrial K(ATP) channels triggers isoflurane-induced preconditioning by generating reactive oxygen species (ROS) in vivo. METHODS: Pentobarbital-anesthetized rabbits were subjected ... More
AuthorsYamagishi S, Nakamura K, Matsui T, Inagaki Y, Takenaka K, Jinnouchi Y, Yoshida Y, Matsuura T, Narama I, Motomiya Y, Takeuchi M, Inoue H, Yoshimura A, Bucala R, Imaizumi T
JournalJ Biol Chem
PubMed ID16707486
'Pigment epithelium-derived factor (PEDF) is the most potent inhibitor of angiogenesis, suggesting that loss of PEDF contributes to proliferative diabetic retinopathy. However, the role of PEDF against retinal vascular hyperpermeability remains to be elucidated. We investigated here whether and how PEDF could inhibit the advanced glycation end product (AGE) signaling ... More
Increased superoxide production in coronary arteries in hyperhomocysteinemia: role of tumor necrosis factor-alpha, NAD(P)H oxidase, and inducible nitric oxide synthase.
AuthorsUngvari Z, Csiszar A, Edwards JG, Kaminski PM, Wolin MS, Kaley G, Koller A
JournalArterioscler Thromb Vasc Biol
PubMed ID12615666
'OBJECTIVE: In coronary arteries, hyperhomocysteinemia (HHcy, a known risk factor for coronary heart disease) impairs flow-induced dilations, which can be reversed by superoxide dismutase (SOD). To evidence increased O2*- generation and elucidate its source, we characterized changes in activity (lucigenin chemiluminescence, hydroethidine staining) and expression of arterial pro- and antioxidant ... More
Cell death-associated translocation of plasma membrane components induced by CTL.
AuthorsKawasaki Y, Saito T, Shirota-Someya Y, Ikegami Y, Komano H, Lee MH, Froelich CJ, Shinohara N, Takayama H
JournalJ Immunol
PubMed ID10779768
'In the very early stages of target cell apoptosis induced by CTL, we found that fluorescence of labeling probes of the target plasma membrane, such as N-(3-triethylammoniumpropyl)-4-(p-dibutylaminostyryl)pyridin ium dibromide (FM1-43), was translocated into intracellular membrane structures including nuclear envelope and mitochondria. This translocation was associated with the execution of CTL-mediated ... More
Vanadium-induced nuclear factor of activated T cells activation through hydrogen peroxide.
AuthorsHuang C, Ding M, Li J, Leonard SS, Rojanasakul Y, Castranova V, Vallyathan V, Ju G, Shi X
JournalJ Biol Chem
PubMed ID11292823
'The present study investigated the role of reactive oxygen species (ROS) in activation of nuclear factor of activated T cells (NFAT), a pivotal transcription factor responsible for regulation of cytokines, by vanadium in mouse embryo fibroblast PW cells or mouse epidermal Cl 41 cells. Exposure of cells to vanadium led ... More
Critical evaluation of the use of hydroethidine as a measure of superoxide anion radical.
AuthorsBenov L, Sztejnberg L, Fridovich I
JournalFree Radic Biol Med
PubMed ID9823548
'The fluorogenic oxidation of hydroethidine (HE) to ethidium (E+) has been used as a measure of O2-. Evaluation of this method confirms that O2-, but not O2 or H2O2, rapidly oxidizes HE to E+. However the ratio of E+ produced per O2- introduced decreased as the flux of O2- was ... More
Flow cytometric quantitation of human opsonin-dependent phagocytosis and oxidative burst responses to meningococcal antigens.
AuthorsLehmann AK, Halstensen A, Bassøe CF
JournalCytometry
PubMed ID9845434
'A one-step flow cytometric (FCM) assay has been developed to quantify both opsonin- and antigen-dependent phagocytosis and intraphagocyte oxidative burst responses. Meningococcal outer membrane structures (OMV) were adsorbed to fluorescent polystyrene beads, opsonized with serum, and exposed to leukocytes. FCM parameters of phagocytosis were evaluated in combinations with oxidative burst ... More
NAD(P)H oxidase mediates the endothelial barrier dysfunction induced by TNF-alpha.
AuthorsGertzberg N, Neumann P, Rizzo V, Johnson A
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID12807699
'We tested the hypothesis that the NAD(P)H oxidase-dependent generation of superoxide anion (O2-*) mediates tumor necrosis factor-alpha (TNF)-induced alterations in the permeability of pulmonary microvessel endothelial monolayers (PMEM). The permeability of PMEM was assessed by the clearance rate of Evans blue-labeled albumin. The NAD(P)H oxidase subcomponents p47phox and p22phox were ... More
Mapping mechanisms and charting the time course of premature cell senescence and apoptosis: lysosomal dysfunction and ganglioside accumulation in endothelial cells.
AuthorsPatschan S, Chen J, Gealekman O, Krupincza K, Wang M, Shu L, Shayman JA, Goligorsky MS,
JournalAm J Physiol Renal Physiol
PubMed ID17928415
'Endothelial cells subjected to glycated collagen I develop premature senescence within 3-5 days, as revealed by increased senescence-associated beta-galactosidase activity, decreased proliferation, and an increase in cell size. Here, we analyzed the time course and possible mechanisms of this process. Lysosomal integrity studies revealed a rapid collapse of pH gradient ... More
Accurate measure of laser irradiance threshold for near-infrared photo-oxidation with a modified confocal microscope.
AuthorsDenton ML, Schuster KJ, Rockwell BA
JournalJ Microsc
PubMed ID16551277
'Femtosecond mode-locked lasers are now being used routinely in multiphoton fluorescence and autofluorescence spectroscopy, are just beginning to be used in refractive surgery, and may be used in the future diagnosis of skin cancer. Pulses from these lasers induce non-linear effects in resultant tissue interactions. Using a modified confocal microscope ... More
L-4F, an apolipoprotein A-1 mimetic, dramatically improves vasodilation in hypercholesterolemia and sickle cell disease.
AuthorsOu J, Ou Z, Jones DW, Holzhauer S, Hatoum OA, Ackerman AW, Weihrauch DW, Gutterman DD, Guice K, Oldham KT, Hillery CA, Pritchard KA
JournalCirculation
PubMed ID12732610
'BACKGROUND: Hypercholesterolemia and sickle cell disease (SCD) impair endothelium-dependent vasodilation by dissimilar mechanisms. Hypercholesterolemia impairs vasodilation by a low-density lipoprotein (LDL)-dependent mechanism. SCD has been characterized as a chronic state of inflammation in which xanthine oxidase (XO) from ischemic tissues increases vascular superoxide anion (O2*-) generation. Recent reports indicate that ... More
Mutagenicity of nitric oxide-releasing compounds in Escherichia coli: effect of superoxide generation and evidence for two mutagenic mechanisms.
AuthorsMartínez A, Urios A, Felipo V, Blanco M
JournalMutat Res
PubMed ID11525919
'The mutagenicity of three nitric oxide (NO) donors, 3-morpholinosydnonimine (SIN-1), a compound generating the precursors of peroxynitrite NO and superoxide, diethylamine/NO (DEA/NO) and spermine/NO (SPER/NO), both releasing authentic NO was analyzed using Escherichia coli tester strains IC203, carrying a deletion of the oxyR gene, and its oxyR(+) parent IC188 (the ... More
Demonstration of functionally distinct human polymorphonuclear leukocyte fractions by simultaneous measurement of phagocytosis and oxygen radical generation.
AuthorsTakano K, Sasada M, Harakawa N, Nogawa M, Asagoe K, Yamamoto K, Okuma M
JournalInt J Hematol
PubMed ID9220662
'Phagocytosis and oxygen radical generation by human polymorphonuclear leukocytes (PMNLs) were studied by a two-color flow cytometric analysis, where the red fluorescent product(s) of hydroethidine was used as an indicator of intracellular generation of oxygen radicals and opsonized zymosan (OZ) as an indicator of phagocytosis. Unstimulated cells formed a single ... More
Role of beta3 integrins in melanoma cell adhesion to activated platelets under flow.
AuthorsFelding-Habermann B, Habermann R, SaldÃvar E, Ruggeri ZM
JournalJ Biol Chem
PubMed ID8621462
'Mechanisms mediating tumor cell attachment to the vessel wall under flow conditions are largely unknown. Therefore we analyzed the ability of human melanoma cells to adhere to an immobilized matrix during blood flow and determined the role of platelets in this process. In a parallel plate flow chamber, M21 melanoma ... More
Stimulation of the pentose phosphate pathway and glutathione levels by dehydroascorbate, the oxidized form of vitamin C.
AuthorsPuskas F, Gergely P, Banki K, Perl A
JournalFASEB J
PubMed ID10877828
'Ascorbic acid, or vitamin C, generally functions as an antioxidant by directly reacting with reactive oxygen intermediates and has a vital role in defenses against oxidative stress. However, ascorbic acid also has pro-oxidant properties and may cause apoptosis of lymphoid and myeloid cells. The present study shows that dehydroascorbate, the ... More
Poly(ADP-ribose) synthetase activation mediates mitochondrial injury during oxidant-induced cell death.
AuthorsVirág L, Salzman AL, Szabó C
JournalJ Immunol
PubMed ID9759901
'Reactive oxidant species are important mediators of tissue injury in shock, inflammation, and reperfusion injury. The actions of a number of these oxidants (e.g., hydroxyl radical and peroxynitrite, a reactive oxidant produced by the reaction of nitric oxide and superoxide) are mediated in part by the activation of the nuclear ... More
Intracellular calcium responses of circadian pacemaker neurons measured with fura-2.
AuthorsGeusz ME, Michel S, Block GD
JournalBrain Res
PubMed ID8199851
'The circadian pacemaker in the eye of the mollusk Bulla gouldiana is located within basal retinal neurons (BRNs) that express a circadian rhythm in cell culture. Light and other depolarizing stimuli shift the phase of the pacemaker in the eye through a process that requires extracellular calcium and is blocked ... More
Simultaneous flow cytometric method to measure phagocytosis and oxidative products by neutrophils.
AuthorsPerticarari S, Presani G, Mangiarotti MA, Banfi E
JournalCytometry
PubMed ID1782835
'We developed a rapid and sensitive two-color flow cytometric method which allows the simultaneous quantification of both the phagocytosis rate and the oxidative burst activation of polymorphonuclear leukocytes (PMNLs). The oxidation of hydroethidine (HE) to ethidium bromide (EB) was performed by the oxidative neutrophil products within the cells during the ... More
Gene transfer of endothelial nitric oxide synthase reduces angiotensin II-induced endothelial dysfunction.
AuthorsNakane H, Miller FJ, Faraci FM, Toyoda K, Heistad DD
JournalHypertension
PubMed ID10679503
'Angiotensin II stimulates vascular NADPH oxidase to produce superoxide, which can react with nitric oxide and impair vasomotor function. We tested the hypothesis that the overexpression of endothelial nitric oxide synthase (eNOS) or superoxide dismutase (SOD) would correct angiotensin II-induced endothelial dysfunction. We examined the effects of the gene transfer ... More
Quantification of effector/target conjugation involving natural killer (NK) or lymphokine activated killer (LAK) cells by two-color flow cytometry.
'Precise estimates of the frequency of NK- and LAK-target conjugates were obtained by two-color flow cytometry using hydroethidine and calcein as intracellular labels for target cells and effector cells, respectively. These two dyes can easily be used with a standard single-laser flow cytometer with excellent signal separation and dye retention. ... More
Mitochondrial complex I inhibitor rotenone induces apoptosis through enhancing mitochondrial reactive oxygen species production.
'Inhibition of mitochondrial respiratory chain complex I by rotenone had been found to induce cell death in a variety of cells. However, the mechanism is still elusive. Because reactive oxygen species (ROS) play an important role in apoptosis and inhibition of mitochondrial respiratory chain complex I by rotenone was thought ... More
Phagocytosis of Staphylococcus aureus and Haemophilus influenzae type B opsonized with polyclonal human IgG1 and IgG2 antibodies. Functional hFc gamma RIIa polymorphism to IgG2.
AuthorsBredius RG, de Vries CE, Troelstra A, van Alphen L, Weening RS, van de Winkel JG, Out TA
JournalJ Immunol
PubMed ID8335940
'To assess the function of IgG subclass antibodies we studied the opsonization of Staphylococcus aureus (STAW) and Haemophilus influenzae type b (Hib) by natural IgG1 and IgG2 antibodies from five healthy individuals. Phagocytosis by polymorphonuclear leukocytes (PMN) was analyzed using FITC-labeled bacteria and flow cytometry. All PMN donors were typed ... More
Copper-zinc superoxide dismutase affects Akt activation after transient focal cerebral ischemia in mice.
AuthorsNoshita N, Sugawara T, Lewén A, Hayashi T, Chan PH
JournalStroke
PubMed ID12738898
'BACKGROUND AND PURPOSE: The serine-threonine kinase Akt is activated by phosphorylation at serine-473. After phosphorylation, activated Akt inactivates BAD or caspase-9 or other apoptogenic components, thereby inhibiting cell death. In this study we examined the relationship between Akt phosphorylation and oxidative stress after transient focal cerebral ischemia (FCI) using copper-zinc ... More
Brain-derived neurotrophic factor can act as a pronecrotic factor through transcriptional and translational activation of NADPH oxidase.
AuthorsKim SH, Won SJ, Sohn S, Kwon HJ, Lee JY, Park JH, Gwag BJ
JournalJ Cell Biol
PubMed ID12460985
'Several lines of evidence suggest that neurotrophins (NTs) potentiate or cause neuronal injury under various pathological conditions. Since NTs enhance survival and differentiation of cultured neurons in serum or defined media containing antioxidants, we set out experiments to delineate the patterns and underlying mechanisms of brain-derived neurotrophic factor (BDNF)-induced neuronal ... More
Selective fluorescent imaging of superoxide in vivo using ethidium-based probes.
AuthorsRobinson KM, Janes MS, Pehar M, Monette JS, Ross MF, Hagen TM, Murphy MP, Beckman JS
JournalProc Natl Acad Sci U S A
PubMed ID17015830
'The putative oxidation of hydroethidine (HE) has become a widely used fluorescent assay for the detection of superoxide in cultured cells. By covalently joining HE to a hexyl triphenylphosphonium cation (Mito-HE), the HE moiety can be targeted to mitochondria. However, the specificity of HE and Mito-HE for superoxide in vivo ... More
Hydrogen peroxide overproduction in megamitochondria of troglitazone-treated human hepatocytes.
AuthorsShishido S, Koga H, Harada M, Kumemura H, Hanada S, Taniguchi E, Kumashiro R, Ohira H, Sato Y, Namba M, Ueno T, Sata M
JournalHepatology
PubMed ID12500198
'Troglitazone has been withdrawn from therapeutic options for diabetes mellitus because of its severe hepatocyte toxicity of unknown pathogenesis. The aim of the present study was to assess both morphologic and functional alterations in the mitochondria of troglitazone-treated hepatocytes. A polarized human hepatocyte cell line, OUMS-29, was used in this ... More
Pseudomonas aeruginosa induces apoptosis in human endothelial cells.
AuthorsValente E, Assis MC, Alvim IM, Pereira GM, Plotkowski MC
JournalMicrob Pathog
PubMed ID11095919
'Pseudomonas aeruginosa has been shown to enter into human endothelial cells in vitro. To ascertain the effects of bacterial intracellular (IC) infection, endothelial cells were exposed to PAK and PAO-1 strains for 1 h and treated with gentamicin in culture medium for different periods. P. aeruginosa induced a significant production ... More
Gender differences in superoxide generation in microvessels of hypertensive rats: role of NAD(P)H-oxidase.
'OBJECTIVE: This study is aimed to explore whether gender plays a role in the generation of nitric oxide (NO) and superoxide anion (O(2)(-)) in microvessels of hypertensive rats (SHR), as well as the potential mechanisms involved in these effects. METHODS AND RESULTS: NO generation in mesenteric arterioles was evaluated by ... More
p38 Signaling-mediated hypoxia-inducible factor 1alpha and vascular endothelial growth factor induction by Cr(VI) in DU145 human prostate carcinoma cells.
AuthorsGao N, Jiang BH, Leonard SS, Corum L, Zhang Z, Roberts JR, Antonini J, Zheng JZ, Flynn DC, Castranova V, Shi X
JournalJ Biol Chem
PubMed ID12213806
'Chromium(VI) (Cr(VI)) is widely used in industry and is a potent inducer of tumors in animals. The present study demonstrates that Cr(VI) induces hypoxia-inducible factor 1 (HIF-1) activity through the specific expression of HIF-1alpha but not HIF-1beta subunit and increases the level of vascular endothelial growth factor (VEGF) expression in ... More
A sensitive flow cytometric method for measuring the oxidative burst.
AuthorsModel MA, KuKuruga MA, Todd RF
JournalJ Immunol Methods
PubMed ID9107299
'We report a novel method of flow cytometric detection of the oxidative burst in human neutrophils. The cells were covalently labeled on the plasma membrane by incubation with 4-carboxydihydrotetramethylrosamine succinimidyl ester on ice. Activation of neutrophils resulted in an increase in red fluorescence at 575 nm using 514 nm excitation. ... More
Diamide primes neutrophils for enhanced release of superoxide anion: relationship to S-thiolation of cellular proteins.
AuthorsMoriguchi T, Seres T, Ravichandran V, Sasada M, Johnston RB
JournalJ Leukoc Biol
PubMed ID8773580
'Stimulation of the respiratory burst in phagocytes induces the formation of mixed disulfides between sulfhydryl groups of proteins and low-molecular-weight thiols. We hypothesized that this process (S-thiolation) might be involved in turning off the respiratory burst. However, induction of S-thiolation by pretreatment of neutrophils with diamide, a direct thiol oxidizing ... More
Aberrant redox regulation in human metastatic melanoma cells compared to normal melanocytes.
AuthorsMeyskens FL, McNulty SE, Buckmeier JA, Tohidian NB, Spillane TJ, Kahlon RS, Gonzalez RI
JournalFree Radic Biol Med
PubMed ID11557318
'Melanocytes and melanoma cells contain melanin, a complex polymer that modulates redox changes in these cells. Relative intracellular hydrogen peroxide levels measured by dichlorodihydrofluorescein are similar in the two cell types, but the levels of superoxide anion measured by dihydroethidium were markedly increased in melanoma cells. Chelator-induced oxidative stress is ... More
Effects of pyrroloquinoline quinone on glutamate-induced production of reactive oxygen species in neurons.
AuthorsScanlon JM, Aizenman E, Reynolds IJ
JournalEur J Pharmacol
PubMed ID9178657
'Pyrroloquinoline quinone may act as a free radical scavenger and also as a modulator of the NMDA receptor associated redox modulatory site. Using the oxidation sensitive dye dihydroethidium, we examined the effects of pyrroloquinoline quinone on free radical production in cultured forebrain neurons following glutamate receptor activation. Both glutamate (100 ... More
The vascular NADPH oxidase subunit p47phox is involved in redox-mediated gene expression.
AuthorsBrandes RP, Miller FJ, Beer S, Haendeler J, Hoffmann J, Ha T, Holland SM, Görlach A, Busse R
JournalFree Radic Biol Med
PubMed ID12031896
'An NADPH oxidase is thought to be a main source of vascular superoxide (O(2)(-)) production. The functional role of this oxidase, however, and the contribution of the different subunits of the enzyme to cellular signaling are still incompletely understood. We determined the role of the p47phox subunit of the oxidase ... More
Divergent roles of angiotensin II AT1 and AT2 receptors in modulating coronary microvascular function.
AuthorsZhang C, Hein TW, Wang W, Kuo L
JournalCirc Res
PubMed ID12595345
'Angiotensin II (Ang II) is a potent vasoconstrictor in the peripheral circulation and has been implicated in many cardiovascular diseases associated with elevated oxidative stress. However, its direct vasomotor action and its linkage to oxidative stress-induced vascular dysfunction in the coronary microcirculation remain elusive. In this study, we directly assessed ... More
Resolution of mitochondrial oxidative stress rescues coronary collateral growth in Zucker obese fatty rats.
'We have previously found abrogated ischemia-induced coronary collateral growth in Zucker obese fatty (ZOF) rats compared with Zucker lean (ZLN) rats. Because ZOF rats have structural abnormalities in their mitochondria suggesting dysfunction and also show increased production of O(2), we hypothesized that mitochondrial dysfunction caused by oxidative stress impairs coronary ... More
AuthorsGeijtenbeek TB, Krooshoop DJ, Bleijs DA, van Vliet SJ, van Duijnhoven GC, Grabovsky V, Alon R, Figdor CG, van Kooyk Y
JournalNat Immunol
PubMed ID11017109
'Dendritic cells (DCs) are recruited from blood into tissues to patrol for foreign antigens. After antigen uptake and processing, DCs migrate to the secondary lymphoid organs to initiate immune responses. We now show that DC-SIGN, a DC-specific C-type lectin, supports tethering and rolling of DC-SIGN-positive cells on the vascular ligand ... More
Abrogation of nuclear factor-kappaB activation is involved in zinc inhibition of lipopolysaccharide-induced tumor necrosis factor-alpha production and liver injury.
AuthorsZhou Z, Wang L, Song Z, Saari JT, McClain CJ, Kang YJ
JournalAm J Pathol
PubMed ID15111301
'Endotoxin (lipopolysaccharide, LPS)-induced tumor necrosis factor-alpha (TNF-alpha) release from Kupffer cells is critically involved in the pathogenesis of alcohol-induced liver injury. We recently reported that inhibition of alcohol-induced plasma endotoxin elevation contributes to the protective action of zinc against alcoholic hepatotoxicity. The present study was undertaken to determine whether zinc ... More
Selective down-regulation of alveolar macrophage oxidative response to opsonin-independent phagocytosis.
AuthorsKobzik L, Godleski JJ, Brain JD
JournalJ Immunol
PubMed ID2160498
'We have compared the oxidative response of alveolar macrophages (AM) during opsonin-dependent and independent phagocytosis by using multiparameter flow cytometry. The respiratory burst of AM during phagocytosis was quantitated by the intracellular oxidation of the nonfluorescent precursors dichlorofluorescin diacetate (DCFH) or hydroethidine (HE, a reduced precursor of ethidium) to their ... More
The glomerulosclerosis gene Mpv17 encodes a peroxisomal protein producing reactive oxygen species.
AuthorsZwacka RM, Reuter A, Pfaff E, Moll J, Gorgas K, Karasawa M, Weiher H
JournalEMBO J
PubMed ID7957077
'The mutant mouse strain Mpv17 carries a retroviral insert in its genome which inactivates the Mpv17 gene. At a young age these mice develop glomerulosclerosis and nephrotic syndrome which resembles human disease. We show here that the Mpv17 gene product is highly conserved and encodes a peroxisomal protein. Loss of ... More
Phosphoaspirin (MDC-43), a novel benzyl ester of aspirin, inhibits the growth of human cancer cell lines more potently than aspirin: a redox-dependent effect.
'Aspirin is chemopreventive against colon and probably other cancers, but this effect is relatively weak and its chronic administration to humans is associated with significant side effects. Because of these limitations, extensive effort has been exerted to improve the pharmacological properties of aspirin. We have determined the anticancer activity and ... More
Fluorescence probes used for detection of reactive oxygen species.
AuthorsGomes A, Fernandes E, Lima JL,
JournalJ Biochem Biophys Methods
PubMed ID16297980
'Endogenously produced pro-oxidant reactive species are essential to life, being involved in several biological functions. However, when overproduced (e.g. due to exogenous stimulation), or when the levels of antioxidants become severely depleted, these reactive species become highly harmful, causing oxidative stress through the oxidation of biomolecules, leading to cellular damage ... More
Continuous cell activation is necessary for stable interaction of complement receptor type 3 with its counter-structure in the aggregation response of human neutrophils.
AuthorsKuypers TW, Koenderman L, Weening RS, Verhoeven AJ, Roos D
JournalEur J Immunol
PubMed ID2180724
'Human neutrophils aggregate after stimulation with various stimuli; this response is completely absent in neutrophils from patients with leukocyte adhesion deficiency (LAD). To investigate the cellular requirements of this process a method was used in which neutrophils are separately loaded with hydroethidine (HE) and sulfofluorescein (SFDA), to give them either ... More
Murine prolylcarboxypeptidase depletion induces vascular dysfunction with hypertension and faster arterial thrombosis.
AuthorsAdams GN, LaRusch GA, Stavrou E, Zhou Y, Nieman MT, Jacobs GH, Cui Y, Lu Y, Jain MK, Mahdi F, Shariat-Madar Z, Okada Y, D'Alecy LG, Schmaier AH,
JournalBlood
PubMed ID21297000
'Prolylcarboxypeptidase (PRCP) activates prekallikrein to plasma kallikrein, leading to bradykinin liberation, and degrades angiotensin II. We now identify PRCP as a regulator of blood vessel homeostasis. ß-Galactosidase staining in PRCP(gt/gt) mice reveals expression in kidney and vasculature. Invasive telemetric monitorings show that PRCP(gt/gt) mice have significantly elevated blood pressure. PRCP(gt/gt) ... More
Blockade of the apoptotic machinery by cyclosporin A redirects cell death toward necrosis in arterial endothelial cells: regulation by reactive oxygen species and cathepsin D.
AuthorsRaymond MA, Mollica L, Vigneault N, Désormeaux A, Chan JS, Filep JG, Hébert MJ
JournalFASEB J
PubMed ID12514115
'Blockade of the mitochondrial permeability transition pore (mPTP) by cyclosporin A (CsA) inhibits apoptosis in various cell types. However, use of CsA in humans is associated with damage to the arterial endothelium. We evaluated whether inhibition of the apoptotic machinery by CsA promotes other forms of cell death in arterial ... More
IL-10 deficiency increases superoxide and endothelial dysfunction during inflammation.
AuthorsGunnett CA, Heistad DD, Berg DJ, Faraci FM
JournalAm J Physiol Heart Circ Physiol
PubMed ID11009441
'Little is known about the role of interleukin-10 (IL-10), an anti-inflammatory cytokine, in blood vessels. We used IL-10-deficient mice (IL-10 -/-) to examine the hypothesis that IL-10 protects endothelial function after lipopolysaccharide (LPS) treatment. The responses of carotid arteries were studied in vitro 6 h after injection of a relatively ... More
Outer membrane vesicles from Neisseria meningitidis.
AuthorsMirlashari MR, Høiby EA, Holst J, Lyberg T
JournalAPMIS
PubMed ID12076272
'Flow cytometry was used to study the expression of leukocyte adhesion molecules CD11a, CD11b, CD11c, CD14, and CD62L (L-selectin) and production of reactive oxygen species (ROS) in an ex vivo human whole-blood system stimulated with lipopolysaccharide-containing outer membrane vesicles (LPS-OMV) from N. meningitidis. Results demonstrated a dose-dependent increase in surface ... More
Amplification of a reactive oxygen species signal in axotomized retinal ganglion cells.
AuthorsNguyen SM, Alexejun CN, Levin LA
JournalAntioxid Redox Signal
PubMed ID14580319
'Retinal ganglion cells (RGCs) undergo apoptosis after axonal injury. Elucidation of the sequence of intracellular events proximal to caspase activation may allow development of effective neuroprotective strategies. In this study, we explored the role that reactive oxygen species may have in signaling RGC apoptosis after axonal injury. Using the fluorescent ... More
Role of superoxide, nitric oxide, and peroxynitrite in doxorubicin-induced cell death in vivo and in vitro.
AuthorsMukhopadhyay P, Rajesh M, Bátkai S, Kashiwaya Y, Haskó G, Liaudet L, Szabó C, Pacher P,
JournalAm J Physiol Heart Circ Physiol
PubMed ID19286953
'Doxorubicin (DOX) is a potent available antitumor agent; however, its clinical use is limited because of its cardiotoxicity. Cell death is a key component in DOX-induced cardiotoxicity, but its mechanisms are elusive. Here, we explore the role of superoxide, nitric oxide (NO), and peroxynitrite in DOX-induced cell death using both ... More
An improved double fluorescence flow cytometry method for the quantification of killer cell/target cell conjugate formation.
AuthorsCavarec L, Quillet-Mary A, Fradelizi D, Conjeaud H
JournalJ Immunol Methods
PubMed ID2197333
'We have developed an improved method to analyse stable associations (conjugate formation) between effector and target cells. Hydroethidine (red) stained lymphoblastoid target cells were cocentrifuged with carboxyfluorescein diacetate acetoxymethylester (green) stained human IL-2 activated cytotoxic cells (LAK). In the present studies either enriched or purified CD3 negative large granular lymphocytes ... More
Vanadate-induced expression of hypoxia-inducible factor 1 alpha and vascular endothelial growth factor through phosphatidylinositol 3-kinase/Akt pathway and reactive oxygen species.
AuthorsGao N, Ding M, Zheng JZ, Zhang Z, Leonard SS, Liu KJ, Shi X, Jiang BH
JournalJ Biol Chem
PubMed ID12070140
'Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric basic helix-loop-helix transcription factor composed of HIF-1 alpha and HIF-1 beta/aryl hydrocarbon nuclear translocator subunits. HIF-1 expression is induced by hypoxia, growth factors, and activation of oncogenes. In response to hypoxia, HIF-1 activates the expression of many genes including vascular endothelial growth factor ... More
Elevated glucose concentrations promote receptor-independent activation of adherent human neutrophils: an experimental and computational approach.
AuthorsKummer U, Zobeley J, Brasen JC, Fahmy R, Kindzelskii AL, Petty AR, Clark AJ, Petty HR
JournalBiophys J
PubMed ID17237194
'Neutrophil activation plays integral roles in host tissue damage and resistance to infectious diseases. As glucose uptake and NADPH availability are required for reactive oxygen metabolite production by neutrophils, we tested the hypothesis that pathological glucose levels (>or=12 mM) are sufficient to activate metabolism and reactive oxygen metabolite production in ... More
ANG II signaling in vasa recta pericytes by PKC and reactive oxygen species.
AuthorsZhang Z, Rhinehart K, Kwon W, Weinman E, Pallone TL
JournalAm J Physiol Heart Circ Physiol
PubMed ID15072960
'ANG II constricts descending vasa recta (DVR) through Ca(2+) signaling in pericytes. We examined the role of PKC DVR pericytes isolated from the rat renal outer medulla. The PKC blocker staurosporine (10 microM) eliminated ANG II (10 nM)-induced vasoconstriction, inhibited pericyte cytoplasmic Ca(2+) concentration ([Ca(2+)](cyt)) elevation, and blocked Mn(2+) influx ... More
Sevoflurane exposure generates superoxide but leads to decreased superoxide during ischemia and reperfusion in isolated hearts.
'Reactive oxygen species (ROS) are largely responsible for cardiac injury consequent to ischemia and reperfusion, but, paradoxically, there is evidence suggesting that anesthetics induce preconditioning (APC) by generating ROS. We hypothesized that sevoflurane generates the ROS superoxide (O(2)(.-)), that APC attenuates O(2)(.-) formation during ischemia, and that this attenuation is ... More
Retention of vital dyes correlates inversely with the multidrug-resistant phenotype of adriamycin-selected murine fibrosarcoma variants.
AuthorsBucana CD, Giavazzi R, Nayar R, O'Brian CA, Seid C, Earnest LE, Fan D
JournalExp Cell Res
PubMed ID2387329
'Retention of the vital dyes rhodamine 123 (R-123) and hydroethidine (HET) correlates inversely with the multidrug resistant phenotypes of the adriamycin (ADM)-selected variants of a uv-induced murine fibrosarcoma cell line (UV-2237M). The differential affinity of these dyes for specific cellular organelles makes them unique compounds for studies of cellular transport. ... More
Trypanosoma cruzi induces the reactive oxygen species-PARP-1-RelA pathway for up-regulation of cytokine expression in cardiomyocytes.
AuthorsBa X, Gupta S, Davidson M, Garg NJ,
JournalJ Biol Chem
PubMed ID20145242
'In this study, we demonstrate that human cardiomyocytes (AC16) produce reactive oxygen species (ROS) and inflammatory cytokines in response to Trypanosoma cruzi. ROS were primarily produced by mitochondria, some of which diffused to cytosol of infected cardiomyocytes. These ROS resulted in an increase in 8-hydroxyguanine lesions and DNA fragmentation that ... More
Free radical-mediated cell damage after experimental status epilepticus in hippocampal slice cultures.
AuthorsKovács R, Schuchmann S, Gabriel S, Kann O, Kardos J, Heinemann U
JournalJ Neurophysiol
PubMed ID12466417
'Generation of free radicals may have a key role in the nerve cell damage induced by prolonged or frequently recurring convulsions (status epilepticus). Mitochondrial function may also be altered due to production of free radicals during seizures. We therefore studied changes in field potentials (fp) together with measurements of extracellular, ... More
Reactive oxygen species modulate coronary wall shear stress and endothelial function during hyperglycemia.
AuthorsGross ER, LaDisa JF, Weihrauch D, Olson LE, Kress TT, Hettrick DA, Pagel PS, Warltier DC, Kersten JR
JournalAm J Physiol Heart Circ Physiol
PubMed ID12543642
'Hyperglycemia is associated with generation of reactive oxygen species (ROS), and this action may contribute to accelerated atherogenesis. We tested the hypothesis that hyperglycemia produces alterations in left anterior descending coronary artery (LAD) wall shear stress concomitant with endothelial dysfunction and ROS production in dogs (n = 12) instrumented for ... More
Reactive oxygen species play no role in the candidacidal activity of the salivary antimicrobial peptide histatin 5.
AuthorsVeerman EC, Nazmi K, Van't Hof W, Bolscher JG, Den Hertog AL, Nieuw Amerongen AV
JournalBiochem J
PubMed ID15109304
'The mechanism of action of antimicrobial peptides is still a matter of debate. The formation of ROS (reactive oxygen species) has been suggested to be the crucial step in the fungicidal mechanism of a number of antimicrobial peptides, including histatin 5 and lactoferrin-derived peptides. In the present study we have ... More
'The source of superoxide (O2*-) production and cell-to-cell interactions of O2*- and nitric oxide (NO) in response to angiotensin II (AngII) were studied by fluorescence microscopic techniques to image rat renal outer medullary microtissue strips. Changes in intracellular O2*- were determined by dihydroethidium-ethidium ratios, and NO was determined with 4,5-diaminofluorescein ... More
Inhibitory properties of nucleic acid-binding ligands on protein synthesis.
AuthorsMalina A, Khan S, Carlson CB, Svitkin Y, Harvey I, Sonenberg N, Beal PA, Pelletier J
JournalFEBS Lett
PubMed ID15620694
'The use of small molecule inhibitors in the study of cellular processes is a powerful approach to understanding gene function. During the course of a high throughput screen for novel inhibitors of eukaryotic translation, we identified a number of nucleic acid binding ligands that showed activity in our assay. When ... More
Reactive oxygen species are critical mediators of coronary collateral development in a canine model.
'Recent evidence suggests that reactive oxygen species (ROS) promote proliferation and migration of vascular smooth muscle (VSMC) and endothelial cells (EC). We tested the hypothesis that ROS serve as crucial messengers during coronary collateral development. Dogs were subjected to brief (2 min), repetitive coronary artery occlusions (1/h, 8/day, 21 day ... More
Defective Neutrophil Oxidative Burst in Preterm Newborns on Exposure to Coagulase-Negative <i>Staphylococci</i>.
AuthorsBjorkqvist M, Jurstrand M, Bodin L, Fredlund H, Schollin J
JournalPediatr Res
PubMed ID15071163
The inhibition of superoxide production in EL4 lymphoma cells overexpressing growth hormone.
AuthorsArnold RE, Weigent DA
JournalImmunopharmacol Immunotoxicol
PubMed ID12784910
'A substantial body of research exists to support the production of growth hormone by cells of the immune system. However, the function and mechanism of action of lymphocyte-derived growth hormone remain largely unelucidated. Since, it has been found that exogenous growth hormone (GH) primes neutrophils for the production of reactive ... More