DQ™ 猪皮肤明胶，荧光素偶联物 - 特殊包装

引用和文献 (50)

Invitrogen™

DQ™ 猪皮肤明胶，荧光素偶联物 - 特殊包装

我们的 DQ 明胶是一种能够以较高灵敏度在体外检测蛋白酶活性的荧光底物。这种底物以前仅在我们常用的 EnzChek 明胶酶/胶原酶检测试剂盒 (E-12055) 中提供，由高度淬灭的荧光素标记明胶组成。经过蛋白水解消化后，它会呈现出明亮的绿色荧光，可用于测量酶活性。荧光强度的增加可以通过荧光微孔板读数仪或荧光计进行监测了解更多信息

货号	数量
D12054	5 x 1 mg

货号 D12054

价格（CNY)

5,445.00

添加至购物车

5 x 1 mg

价格（CNY)

5,445.00

添加至购物车

仅供科研使用。不可用于诊断程序。

产品线DQ

数量5 x 1 mg

运输条件室温

底物蛋白酶底物

检测方法荧光

底物属性基于蛋白的底物

目标酶金属蛋白酶

Unit SizeEach

内容与储存

在冷冻冰箱（-5 至 -30°C）中避光储存。

引用和文献 (50)

引用和文献

Abstract

Gelatin in situ zymography on fixed, paraffin-embedded tissue: zinc and ethanol fixation preserve enzyme activity.

Authors:Hadler-Olsen E, Kanapathippillai P, Berg E, Svineng G, Winberg JO, Uhlin-Hansen L,

Journal:J Histochem Cytochem

PubMed ID:19755718

In situ zymography is a method for the detection and localization of enzymatic activity in tissue sections. This method is used with frozen sections because routine fixation of tissue in neutral-buffered formalin inhibits enzyme activity. However, frozen sections present with poor tissue morphology, making precise localization of enzymatic activity difficult ... More

Chlorotoxin inhibits glioma cell invasion via matrix metalloproteinase-2.

Authors:Deshane J, Garner CC, Sontheimer H

Journal:J Biol Chem

PubMed ID:12454020

'Primary brain tumors (gliomas) have the unusual ability to diffusely infiltrate the normal brain thereby evading surgical treatment. Chlorotoxin is a scorpion toxin that specifically binds to the surface of glioma cells and impairs their ability to invade. Using a recombinant His-Cltx we isolated and identified the principal Cltx receptor ... More

Authentic matrix vesicles contain active metalloproteases (MMP). a role for matrix vesicle-associated MMP-13 in activation of transforming growth factor-beta.

Authors:D'Angelo M, Billings PC, Pacifici M, Leboy PS, Kirsch T

Journal:J Biol Chem

PubMed ID:11145962

'Matrix vesicles (MV) play a key role in the initiation of cartilage mineralization. Although many components in these microstructures have been identified, the specific function of each component is still poorly understood. In this study, we show that metalloproteases (MMP), MMP-2, -9, and -13 are associated with MV isolated from ... More

Molecular proximity of seprase and the urokinase-type plasminogen activator receptor on malignant melanoma cell membranes: dependence on beta1 integrins and the cytoskeleton.

Authors:Artym VV, Kindzelskii AL, Chen WT, Petty HR

Journal:Carcinogenesis

PubMed ID:12376466

'Previous studies have shown that several proteolytic enzymes are associated with membrane protrusions at the leading edge of migrating tumor cells. In this study we demonstrate that seprase and the urokinase plasminogen activator receptor (uPAR), co-localize in the plasma membrane of LOX malignant melanoma cells. Cells were labeled with fluorochrome-conjugated ... More

Pancreatic trypsin increases matrix metalloproteinase-9 accumulation and activation during acute intestinal ischemia-reperfusion in the rat.

Authors:Rosário HS, Waldo SW, Becker SA, Schmid-Schönbein GW

Journal:Am J Pathol

PubMed ID:15111317

'Ischemia-reperfusion of the intestine produces a set of inflammatory mediators, the origin of which has recently been shown to involve pancreatic digestive enzymes. Matrix metalloproteinase-9 (MMP-9) participates in a variety of inflammatory processes including myocardial, hepatic, and pancreatic ischemia-reperfusion. In the present study, we explore the role of neutrophil-derived MMP-9 ... More

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