Calcium regulates the association between mitochondria and a smooth subdomain of the endoplasmic reticulum.
AuthorsWang HJ, Guay G, Pogan L, Sauvé R, Nabi IR
JournalJ Cell Biol
PubMed ID10995452
'Association between the ER and mitochondria has long been observed, and the formation of close contacts between ER and mitochondria is necessary for the ER-mediated sequestration of cytosolic calcium by mitochondria. Autocrine motility factor receptor (AMF-R) is a marker for a smooth subdomain of the ER, shown here by confocal ... More
Local positive feedback by calcium in the propagation of intracellular calcium waves.
AuthorsWang SS, Thompson SH
JournalBiophys J
PubMed ID8580312
'In many types of eukaryotic cells, the activation of surface receptors leads to the production of inositol 1,4,5-trisphosphate and calcium release from intracellular stores. Calcium release can occur in complex spatial patterns, including waves of release that traverse the cytoplasm. Fluorescence video microscopy was used to view calcium waves in ... More
A practical guide to the preparation of Ca2+ buffers.
AuthorsBers DM, Patton CW, Nuccitelli R
JournalMethods Cell Biol
PubMed ID8201981
The Cytosolic Ca2+ Concentration Gradient of Sinapis alba Root Hairs as Revealed by Ca2+-Selective Microelectrode Tests and Fura-Dextran Ratio Imaging.
AuthorsFelle HH, Hepler PK
JournalPlant Physiol
PubMed ID12223687
Using Ca2+-selective microelectrodes and fura 2-dextran ratio imaging, the cytosolic free [Ca2+] was measured in Sinapis alba root hair cells. Both methods yielded comparable results, i.e. values between 158 to 251 nM for the basal [Ca2+] of the cells and an elevated [Ca2+] of 446 to 707 nM in the ... More
Properties of neuroprotective cell-permeant Ca2+ chelators: effects on [Ca2+]i and glutamate neurotoxicity in vitro.
AuthorsTymianski M, Charlton MP, Carlen PL, Tator CH
JournalJ Neurophysiol
PubMed ID7823112
1. Cell-permeant Ca2+ chelators such as 1,2-bis-(2-amino-phenoxy)ethane- N,N,N',N'-tetraacetic acid acetoxymethyl ester (BAPTA-AM) protect neurons against excitotoxic and ischemic neuronal injury in vitro and in vivo. Here we provide the first steps toward characterizing the mechanisms by which these agents produce their neuroprotective effects. 2. Cultured mouse spinal neurons were simultaneously ... More
On the roles of Ca2+ diffusion, Ca2+ buffers, and the endoplasmic reticulum in IP3-induced Ca2+ waves.
AuthorsJafri MS, Keizer J
JournalBiophys J
PubMed ID8580358
We have investigated the effects of Ca2+ diffusion, mobile and stationary Ca2+ buffers in the cytosol, and Ca2+ handling by the endoplasmic reticulum on inositol 1,4,5-trisphosphate-induced Ca2+ wave propagation. Rapid equilibration of free and bound Ca2+ is used to describe Ca2+ sequestration by buffers in both the cytosol and endoplasmic ... More