Determination of intramolecular distance distributions in a globular protein by nonradiative excitation energy transfer measurements.
AuthorsAmir D, Haas E
JournalBiopolymers
PubMed ID2420384
In vitro efficacy of morpholino-modified antisense oligomers directed against tumor necrosis factor-alpha mRNA.
Authors Taylor M F; Paulauskis J D; Weller D D; Kobzik L;
JournalJ Biol Chem
PubMed ID8663413
Chemical modification of antisense oligonucleotides to increase nuclease resistance may improve their efficacy within enzyme-rich cellular targets (e.g. macrophages). We evaluated a panel of morpholino antisense oligomers (M-AS) for their ability to inhibit macrophage tumor necrosis factor-alpha (TNF-alpha) release and compared them to phosphodiester (O-AS) and phosphorothioate (S-AS) types of ... More
Spectroscopic, calorimetric, and kinetic demonstration of conformational adaptation in peptide-antibody recognition.
Little is known about the extent to which protein flexibility contributes to antigen-antibody recognition and cross-reactivity. Using short coil peptides (leucine zippers) as model antigens, we demonstrate that a monoclonal antibody can force a noncognate peptide into a conformation that is similar to the conformation of the cognate peptide against ... More
A series of site-specific fluorescently labeled BPTI derivatives prepared by nonselective acylation and chromatographic separations.
AuthorsAmir D, Haas E
JournalInt J Pept Protein Res
PubMed ID2419267
Investigation of conformational transitions and, in particular, the folding/unfolding transitions of globular proteins by means of excitation energy transfer measurements depends on the availability of protein derivatives carrying donor and acceptor probes at well-defined pairs of sites. A series of bovine pancreatic trypsin inhibitor (BPTI) derivatives, each labeled at one ... More