The ligand affinity of proteins measured by isothermal denaturation kinetics.
AuthorsEpps DE, Sarver RW, Rogers JM, Herberg JT, Tomich PK
JournalAnal Biochem
PubMed ID11319816
An isothermal denaturation kinetic method was developed for identifying potential ligands of proteins and measuring their affinity. The method is suitable for finding ligands specific toward proteins of unknown function and for large-scale drug screening. It consists of analyzing the kinetics of isothermal denaturation of the protein-with and without the ... More