A natural ErbB4 isoform that does not activate phosphoinositide 3-kinase mediates proliferation but not survival or chemotaxis.
AuthorsKainulainen V,Sundvall M,Määttä JA,Santiestevan E,Klagsbrun M,Elenius K
JournalThe Journal of biological chemistry
PubMed ID10722704
The p42/p44 MAP kinase pathway prevents apoptosis induced by anchorage and serum removal.
AuthorsLe Gall M,Chambard JC,Breittmayer JP,Grall D,Pouysségur J,Van Obberghen-Schilling E
JournalMolecular biology of the cell
PubMed ID10712523
Anchorage removal like growth factor removal induces apoptosis. In the present study we have characterized signaling pathways that can prevent this cell death using a highly growth factorâ and anchorage-dependent line of lung fibroblasts (CCL39). After anchorage removal from exponentially growing cells, annexin V-FITC labeling can be detected after 8 ... More
Avian flu: influenza virus receptors in the human airway.
AuthorsShinya K,Ebina M,Yamada S,Ono M,Kasai N,Kawaoka Y
JournalNature
PubMed ID16554799
Molecular cloning, chromosomal localization, tissue distribution, and functional expression of the human pancreatic sodium bicarbonate cotransporter.
AuthorsAbuladze N, Lee I, Newman D, Hwang J, Boorer K, Pushkin A, Kurtz I
JournalJ Biol Chem
PubMed ID9651366
We report the cloning, sequence analysis, tissue distribution, functional expression, and chromosomal localization of the human pancreatic sodium bicarbonate cotransport protein (pancreatic NBC (pNBC)). The transporter was identified by searching the human expressed sequence tag data base. An I.M.A.G.E. clone W39298 was identified, and a polymerase chain reaction probe was ... More
Cytokine-regulated expression of E-selectin, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) in human microvascular endothelial cells.
AuthorsHaraldsen G, Kvale D, Lien B, Farstad IN, Brandtzaeg P
JournalJ Immunol
PubMed ID8786319
Endothelial cells (EC) recruit circulating leukocytes to sites of inflammation, partly by expression of endothelial-leukocyte adhesion molecules. Whereas the regulation of some adhesion molecules is well characterized in cultured HUVEC, similar data for microvascular human test systems are limited. We studied the cytokine-regulated expression of vascular cell adhesion molecules E-selectin, ... More
Identification and characterization of two subpopulations of Encephalitozoon intestinalis.
AuthorsHoffman RM, Marshall MM, Polchert DM, Jost BH
JournalAppl Environ Microbiol
PubMed ID12902292
Microsporidia are obligate intracellular protozoa that have been shown to be pathogenic to most living creatures. The development of in vitro cell culture propagation methods has provided researchers with large numbers of spores and facilitated the study of these organisms. Here, we describe heterogeneity within cell culture-propagated Encephalitozoon intestinalis suspensions. ... More
Selective staining by 4', 6-diamidine-2-phenylindole of nanogram quantities of DNA in the presence of RNA on gels.
AuthorsKapuscinski J, Yanagi K
JournalNucleic Acids Res
PubMed ID493114
4', 6-Diamidine-2-phenylindole.2HCl (DAPI) forms fluorescent complexes with double-stranded (ds) DNA but not with ds RNA as shown by fluorescence titration. The widely used dye ethidium bromide (EB) forms fluorescent complexes with both types of nucleic acids. Also, in contrast to EB, DAPI forms much weaker fluorescent complexes with single-stranded DNA ... More
Normal fertilization occurs with eggs lacking the integrin alpha6beta1 and is CD9-dependent.
AuthorsMiller BJ, Georges-Labouesse E, Primakoff P, Myles DG
JournalJ Cell Biol
PubMed ID10851025
'Previous results, based on inhibition of fertilization by an anti-alpha6 integrin mAb (GoH3), suggest that the alpha6beta1 integrin on mouse eggs functions as the receptor for sperm (Almeida, E.A., A.P. Huovila, A.E. Sutherland, L.E. Stephens, P.G. Calarco, L. M. Shaw, A.M. Mercurio, A. Sonnenberg, P. Primakoff, D.G. Myles, and J.M. ... More
CD1d degradation in Chlamydia trachomatis-infected epithelial cells is the result of both cellular and chlamydial proteasomal activity.
AuthorsKawana K, Quayle AJ, Ficarra M, Ibana JA, Shen L, Kawana Y, Yang H, Marrero L, Yavagal S, Greene SJ, Zhang YX, Pyles RB, Blumberg RS, Schust DJ
JournalJ Biol Chem
PubMed ID17215251
'Chlamydia trachomatis is an obligate intracellular pathogen that can persist in the urogenital tract. Mechanisms by which C. trachomatis evades clearance by host innate immune responses are poorly described. CD1d is MHC-like, is expressed by epithelial cells, and can signal innate immune responses by NK and NKT cells. Here we ... More
High-throughput flow cytometry-based assay to identify apoptosis-inducing proteins.
AuthorsSauermann M, Hahne F, Schmidt C, Majety M, Rosenfelder H, Bechtel S, Huber W, Poustka A, Arlt D, Wiemann S
JournalJ Biomol Screen
PubMed ID17478479
'After sequencing the human genome, the challenge ahead is to systematically analyze the functions and disease relation of the proteins encoded. Here the authors describe the application of a flow cytometry-based high-throughput assay to screen for apoptosis-activating proteins in transiently transfected cells. The assay is based on the detection of ... More
Aberrant expression of ID2, a suppressor of B-cell-specific gene expression, in Hodgkin's lymphoma.
AuthorsRenné C, Martin-Subero JI, Eickernjäger M, Hansmann ML, Küppers R, Siebert R, Bräuninger A
JournalAm J Pathol
PubMed ID16877363
'The global loss of B-cell-specific gene expression is a distinctive feature of the Hodgkin-Reed/Sternberg (HRS) cells of classical Hodgkin''s lymphoma (HL). The reasons for this loss remained largely unknown as transcription factors with pleiotropic effects on B-cell-specific gene expression, namely E2A, EBF, and PAX5, are present in primary HRS cells. ... More
Interferon alpha inhibits a Src-mediated pathway necessary for Shigella-induced cytoskeletal rearrangements in epithelial cells.
'Shigella flexneri, the causative agent of bacillary dysentery, has the ability to enter nonphagocytic cells. The interferon (IFN) family of cytokines was found to inhibit Shigella invasion of cultured epithelial cells. We show here that IFN-alpha inhibits a Src-dependent signaling cascade triggered by Shigella that leads to the reorganization of ... More
Aneuploid mosaicism in the developing and adult cerebellar cortex.
AuthorsWestra JW, Peterson SE, Yung YC, Mutoh T, Barral S, Chun J,
JournalJ Comp Neurol
PubMed ID18273885
'Neuroprogenitor cells (NPCs) in several telencephalic proliferative regions of the mammalian brain, including the embryonic cerebral cortex and postnatal subventricular zone (SVZ), display cell division "defects" in normal cells that result in aneuploid adult progeny. Here, we identify the developing cerebellum as a major, nontelencephalic proliferative region of the vertebrate ... More
Receptor for activated C-kinase (RACK-1), a WD motif-containing protein, specifically associates with the human type I IFN receptor.
AuthorsCroze E, Usacheva A, Asarnow D, Minshall RD, Perez HD, Colamonici O
JournalJ Immunol
PubMed ID11046044
'The cytoplasmic domain of the human type I IFN receptor chain 2 (IFNAR2c or IFN-alphaRbetaL) was used as bait in a yeast two-hybrid system to identify novel proteins interacting with this region of the receptor. We report here a specific interaction between the cytoplasmic domain of IFN-alphaRbetaL and a previously ... More
Generation of induced pluripotent stem cells using recombinant proteins.
AuthorsZhou H, Wu S, Joo JY, Zhu S, Han DW, Lin T, Trauger S, Bien G, Yao S, Zhu Y, Siuzdak G, Schöler HR, Duan L, Ding S,
JournalCell Stem Cell
PubMed ID19398399
'Generation of induced pluripotent stem cells (iPSCs) generally involves genetic manipulation and thus the potential for unexpected genetic modifications by the exogenous sequences in the target cells. Here we report generation of protein-induced pluripotent stem cells (piPSCs) from murine embryonic fibroblasts using recombinant cell-penetrating reprogramming proteins. We demonstrated that such ... More
Bile acids stimulate cFLIP phosphorylation enhancing TRAIL-mediated apoptosis.
AuthorsHiguchi H, Yoon JH, Grambihler A, Werneburg N, Bronk SF, Gores GJ
JournalJ Biol Chem
PubMed ID12407100
'Bile acids induce hepatocyte injury by enhancing death receptor-mediated apoptosis. In this study, bile acid effects on TRAIL-mediated apoptosis were examined to gain insight into bile acid potentiation of death receptor signaling. TRAIL-induced apoptosis of HuH-7 cells, stably transfected with a bile acid transporter, was enhanced by bile acids. Caspase ... More
Expanding the multicolor capabilities of basic confocal microscopes by employing red and near-infrared quantum dot conjugates.
AuthorsKingeter LM, Schaefer BC,
JournalBMC Biotechnol
PubMed ID19463154
'BACKGROUND: Confocal microscopy is a widely employed methodology in cellular biology, commonly used for investigating biological organization at the cellular and sub-cellular level. Most basic confocal microscopes are equipped to cleanly discriminate no more than four fluorophores in a given sample, limiting the utility of this method for co-localization, co-expression, ... More
Regulation of beta-amyloid secretion by FE65, an amyloid protein precursor-binding protein.
AuthorsSabo SL, Lanier LM, Ikin AF, Khorkova O, Sahasrabudhe S, Greengard P, Buxbaum JD
JournalJ Biol Chem
PubMed ID10075692
'The principal component of Alzheimer''s amyloid plaques, Abeta, derives from proteolytic processing of the Alzheimer''s amyloid protein precursor (APP). FE65 is a brain-enriched protein that binds to APP. Although several laboratories have characterized the APP-FE65 interaction in vitro, the possible relevance of this interaction to Alzheimer''s disease has remained unclear. ... More
Subzonal organization of olfactory sensory neurons projecting to distinct glomeruli within the mouse olfactory bulb.
AuthorsLevai O, Breer H, Strotmann J
JournalJ Comp Neurol
PubMed ID12619077
'Olfactory sensory neurons located in the nasal neuroepithelium send their axons directly into the olfactory bulb, where they contact the dendrites of second-order neurons in specialized spherical structures called glomeruli; each sensory neuron projects to a single glomerulus. All neurons expressing the same odorant receptor gene are confined to distinct ... More
The dynamin-related GTPase, Dnm1p, controls mitochondrial morphology in yeast.
AuthorsOtsuga D, Keegan BR, Brisch E, Thatcher JW, Hermann GJ, Bleazard W, Shaw JM
JournalJ Cell Biol
PubMed ID9786946
'The Saccharomyces cerevisiae Dnm1 protein is structurally related to dynamin, a GTPase required for membrane scission during endocytosis. Here we show that Dnm1p is essential for the maintenance of mitochondrial morphology. Disruption of the DNM1 gene causes the wild-type network of tubular mitochondrial membranes to collapse to one side of ... More
Haematopoietic stem cells adopt mature haematopoietic fates in ischaemic myocardium.
'Under conditions of tissue injury, myocardial replication and regeneration have been reported. A growing number of investigators have implicated adult bone marrow (BM) in this process, suggesting that marrow serves as a reservoir for cardiac precursor cells. It remains unclear which BM cell(s) can contribute to myocardium, and whether they ... More
Micron-scale spatially patterned, covalently immobilized vascular endothelial growth factor on hydrogels accelerates endothelial tubulogenesis and increases cellular angiogenic responses.
AuthorsLeslie-Barbick JE, Shen C, Chen C, West JL,
JournalTissue Eng Part A
PubMed ID20712418
'Spontaneous formation of endothelial tubules was restricted to patterned micron-scale regions presenting cell adhesion ligands and angiogenic signaling protein on poly(ethylene glycol) hydrogels. Arginine-glycine-aspartic acid-serine (RGDS), an integrin ligand, and vascular endothelial growth factor (VEGF), a rate-limiting signaling protein involved in angiogenesis, were covalently bound through photopolymerization via laser scanning ... More
Inhibition of platelet-derived growth factor-BB-induced receptor activation and fibroblast migration by hyaluronan activation of CD44.
AuthorsLi L, Heldin CH, Heldin P
JournalJ Biol Chem
PubMed ID16809345
'The extracellular matrix molecule hyaluronan was found to suppress platelet-derived growth factor (PDGF) beta-receptor activation and PDGF-BB-induced migration of primary human dermal fibroblasts. The suppressive effect of hyaluronan was neutralized by a monoclonal antibody that specifically inhibits hyaluronan binding to its receptor CD44. Moreover, co-immunoprecipitation experiments showed that the PDGF ... More
Improved efficacy of stem cell labeling for magnetic resonance imaging studies by the use of cationic liposomes.
Authorsvan den Bos EJ, Wagner A, Mahrholdt H, Thompson RB, Morimoto Y, Sutton BS, Judd RM, Taylor DA
JournalCell Transplant
PubMed ID14653621
'Labeling stem cells with FDA-approved superparamagnetic iron oxide particles makes it possible to track cells in vivo with magnetic resonance imaging (MRI), but high intracellular levels of iron can cause free radical formation and cytotoxicity. We hypothesized that the use of cationic liposomes would increase labeling efficiency without toxic effects. ... More
Identification of ERSE-II, a new cis-acting element responsible for the ATF6-dependent mammalian unfolded protein response.
AuthorsKokame K, Kato H, Miyata T
JournalJ Biol Chem
PubMed ID11112790
'Herp is a 54-kDa membrane protein in the endoplasmic reticulum (ER). The mRNA expression level of Herp is increased by the accumulation of unfolded proteins in the ER. Transcriptional changes designed to deal with this type of ER stress is called the unfolded protein response (UPR). Most mammalian UPR-target genes ... More
Live-cell imaging of rhabdovirus-induced morphological changes in plant nuclear membranes.
AuthorsGoodin M, Yelton S, Ghosh D, Mathews S, Lesnaw J
JournalMol Plant Microbe Interact
PubMed ID16042016
'Potato yellow dwarf virus (PYDV) and Sonchus yellow net virus (SYNV) belong to the genus Nucleorhabdovirus. These viruses replicate in nuclei of infected cells and mature virions accumulate in the perinuclear space after budding through the inner nuclear membrane. Infection of transgenic Nicotiana benthamiana 16c plants (which constitutively express green ... More
Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation.
AuthorsHirsch JD, Eslamizar L, Filanoski BJ, Malekzadeh N, Haugland RP, Beechem JM, Haugland RP
JournalAnal Biochem
PubMed ID12419349
'The high-affinity binding of biotin to avidin, streptavidin, and related proteins has been exploited for decades. However, a disadvantage of the biotin/biotin-binding protein interaction is that it is essentially irreversible under physiological conditions. Desthiobiotin is a biotin analogue that binds less tightly to biotin-binding proteins and is easily displaced by ... More
Signaling by insulin-like growth factors in paralyzed skeletal muscle: rapid induction of IGF1 expression in muscle fibers and prevention of interstitial cell proliferation by IGF-BP5 and IGF-BP4.
AuthorsCaroni P, Schneider C
JournalJ Neurosci
PubMed ID7514217
'In the absence of muscle activity, muscle fibers, muscle interstitial cells, and intramuscular nerves display characteristic reactions presumably aimed at restoring a functioning neuromuscular system and avoiding degenerative events. In partially denervated muscle these include proliferation of interstitial cells, followed by nerve sprouting. The same reactions can be induced in ... More
Alpha5beta1 integrin controls cyclin D1 expression by sustaining mitogen-activated protein kinase activity in growth factor-treated cells.
'Cyclin D1 expression is jointly regulated by growth factors and cell adhesion to the extracellular matrix in many cell types. Growth factors are thought to regulate cyclin D1 expression because they stimulate sustained extracellular signal-regulated kinase (ERK) activity. However, we show here that growth factors induce transient ERK activity when ... More
Multipotent stem cells from trabecular meshwork become phagocytic TM cells.
AuthorsDu Y, Roh DS, Mann MM, Funderburgh ML, Funderburgh JL, Schuman JS,
JournalInvest Ophthalmol Vis Sci
PubMed ID22297497
'To isolate and characterize stem cells from human trabecular meshwork (TM) and to investigate the potential of these stem cells to differentiate into TM cells. Human trabecular meshwork stem cells (TMSCs) were isolated as side population cells by fluorescence-activated cell sorting or isolated by clonal cultures. Passaged TMSCs were compared ... More
Translocation of SAPK/JNK to mitochondria and interaction with Bcl-x(L) in response to DNA damage.
AuthorsKharbanda S, Saxena S, Yoshida K, Pandey P, Kaneki M, Wang Q, Cheng K, Chen YN, Campbell A, Sudha T, Yuan ZM, Narula J, Weichselbaum R, Nalin C, Kufe D
JournalJ Biol Chem
PubMed ID10617621
'Activation of the stress-activated protein kinase (SAPK/JNK) by genotoxic agents is necessary for induction of apoptosis. We report here that ionizing radiation ionizing radiation exposure induces translocation of SAPK to mitochondria and association of SAPK with the anti-apoptotic Bcl-x(L) protein. SAPK phosphorylates Bcl-x(L) on threonine 47 (Thr-47) and threonine 115 ... More
Rapid visualization of microtubules in blood cells and other cell types in marine model organisms.
AuthorsLee KG, Braun A, Chaikhoutdinov I, DeNobile J, Conrad M, Cohen W
JournalBiol Bull
PubMed ID12414579
Helicobacter pylori evolution during progression from chronic atrophic gastritis to gastric cancer and its impact on gastric stem cells.
AuthorsGiannakis M, Chen SL, Karam SM, Engstrand L, Gordon JI,
JournalProc Natl Acad Sci U S A
PubMed ID18332421
'We have characterized the adaptations of Helicobacter pylori to a rarely captured event in the evolution of its impact on host biology-the transition from chronic atrophic gastritis (ChAG) to gastric adenocarcinoma-and defined the impact of these adaptations on an intriguing but poorly characterized interaction between this bacterium and gastric epithelial ... More
Targeting of the c-Abl tyrosine kinase to mitochondria in the necrotic cell death response to oxidative stress.
AuthorsKumar S, Bharti A, Mishra NC, Raina D, Kharbanda S, Saxena S, Kufe D
JournalJ Biol Chem
PubMed ID11350980
'The ubiquitously expressed c-Abl tyrosine kinase is activated in the response of cells to genotoxic and oxidative stress. The present study demonstrates that reactive oxygen species (ROS) induce targeting of c-Abl to mitochondria. We show that ROS-induced localization of c-Abl to mitochondria is dependent on activation of protein kinase C ... More
Rac1 inhibits myogenic differentiation by preventing the complete withdrawal of myoblasts from the cell cycle.
AuthorsHeller H, Gredinger E, Bengal E
JournalJ Biol Chem
PubMed ID11489882
'The small GTPase protein Rac1 is involved in a wide range of biological processes, yet its role in cell differentiation is mostly unknown. Here we show that Rac1 activity is high in proliferating myoblasts and decreases during the differentiation process. To analyze the involvement of Rac1 in muscle differentiation, different ... More
Targeting organic anion transporter 3 with probenecid as a novel anti-influenza a virus strategy.
AuthorsPerwitasari O, Yan X, Johnson S, White C, Brooks P, Tompkins SM, Tripp RA,
JournalAntimicrob Agents Chemother
PubMed ID23129053
'Influenza A virus infection is a major global health concern causing significant mortality, morbidity, and economic loss. Antiviral chemotherapeutics that target influenza A virus are available; however, rapid emergence of drug-resistant strains has been reported. Consequently, there is a burgeoning need to identify novel anti-influenza A drugs, particularly those that ... More
Monitoring yeast spindles in the fluorescence microscope.
AuthorsBaumstark-Khan C, Rink H, Zimmermann HP
JournalEur J Cell Biol
PubMed ID6698040
'Formation of the complete spindles during the budding process of Saccharomyces uvarum was investigated by fluorescence microscopy of protoplasted cells. Protoplasts were treated with anti-tubulin antibodies and DAPI, a fluorescent dye staining DNA. Thus, both chromatin and spindles could be visualized. Duplication as well as formation of separated spindle pole ... More
Compaction of the Escherichia coli nucleoid caused by Cyt1Aa.
AuthorsManasherob R, Zaritsky A, Metzler Y, Ben-Dov E, Itsko M, Fishov I
JournalMicrobiology
PubMed ID14663087
'Compaction of the Escherichia coli nucleoid in the cell''s centre was associated with the loss of colony-forming ability; these effects were caused by induction of Cyt1Aa, the cytotoxic 27 kDa protein from Bacillus thuringiensis subsp. israelensis. Cyt1Aa-affected compaction of the nucleoids was delayed but eventually more intense than compaction caused ... More
Proteome analysis and functional expression identify mortalin as an antiapoptotic gene induced by elevation of [Na+]i/[K+]i ratio in cultured vascular smooth muscle cells.
'Apoptosis of vascular smooth muscle cells (VSMCs) plays an important role in remodeling of vessel walls, one of the major determinants of long-term blood pressure elevation and an independent risk factor for cardiovascular morbidity and mortality. Recently, we have found that apoptosis in cultured VSMCs can be inhibited by inversion ... More
Pygmy squids and giant brains: mapping the complex cephalopod CNS by phalloidin staining of vibratome sections and whole-mount preparations.
AuthorsWollesen T, Loesel R, Wanninger A,
JournalJ Neurosci Methods
PubMed ID19428513
'Among bilaterian invertebrates, cephalopod molluscs (e.g., squids, cuttlefish and octopuses) have a central nervous system (CNS) that rivals in complexity that of the phylogenetically distant vertebrates (e.g., mouse and human). However, this prime example of convergent evolution has rarely been the subject of recent developmental and evolutionary studies, which may ... More
Multiple levels of control regulate the yeast cAMP-response element-binding protein repressor Sko1p in response to stress.
AuthorsPascual-Ahuir A, Posas F, Serrano R, Proft M
JournalJ Biol Chem
PubMed ID11500510
'The Sko1p transcriptional repressor regulates a subset of osmoinducible stress defense genes in Saccharomyces cerevisiae by binding to cAMP-responsive elements. We have reported previously that in response to stress Sko1p is phosphorylated by the stress-activated Hog1p mitogen-activated protein kinase, which disrupts its interaction with the Ssn6p x Tup1p corepressor. Here ... More
Intracellular trafficking of Clostridium perfringens iota-toxin b.
AuthorsNagahama M, Umezaki M, Tashiro R, Oda M, Kobayashi K, Shibutani M, Takagishi T, Ishidoh K, Fukuda M, Sakurai J,
JournalInfect Immun
PubMed ID22825447
'Clostridium perfringens iota-toxin is composed of an enzymatic component (Ia) and a binding component (Ib). Ib binds to a cell surface receptor, undergoes oligomerization in lipid rafts, and binds Ia. The resulting complex is then endocytosed. Here, we show the intracellular trafficking of iota-toxin. After the binding of the Ib ... More
Inhibition of wild-type p66ShcA in mesangial cells prevents glycooxidant-dependent FOXO3a regulation and promotes the survival phenotype.
AuthorsChintapalli J, Yang S, Opawumi D, Goyal SR, Shamsuddin N, Malhotra A, Reiss K, Meggs LG,
JournalAm J Physiol Renal Physiol
PubMed ID17077388
'Hyperglycemia triggers an exponential increase in reactive oxygen species (ROS) at the cellular level. Here, we demonstrate induction of the oxidant-resistant phenotype in mesangial cells by silencing the wild-type (WT) p66ShcA gene. Two approaches were employed to inhibit WTp66ShcA in SV40 murine mesangial cells and normal human mesangial cells: transient ... More
Toward the generation of rod and cone photoreceptors from mouse, monkey and human embryonic stem cells.
AuthorsOsakada F, Ikeda H, Mandai M, Wataya T, Watanabe K, Yoshimura N, Akaike A, Akaike A, Sasai Y, Takahashi M,
JournalNat Biotechnol
PubMed ID18246062
'We previously reported the differentiation of mouse embryonic stem (ES) cells into retinal progenitors. However, these progenitors rarely differentiate into photoreceptors unless they are cultured with embryonic retinal tissues. Here we show the in vitro generation of putative rod and cone photoreceptors from mouse, monkey and human ES cells by ... More
Exportin-5, a novel karyopherin, mediates nuclear export of double-stranded RNA binding proteins.
AuthorsBrownawell AM, Macara IG
JournalJ Cell Biol
PubMed ID11777942
'We have identified a novel human karyopherin (Kap) beta family member that is related to human Crm1 and the Saccharomyces cerevisiae protein, Msn5p/Kap142p. Like other known transport receptors, this Kap binds specifically to RanGTP, interacts with nucleoporins, and shuttles between the nuclear and cytoplasmic compartments. We report that interleukin enhancer ... More
A novel zinc finger protein TReP-132 interacts with CBP/p300 to regulate human CYP11A1 gene expression.
AuthorsGizard F, Lavallée B, DeWitte F, Hum DW
JournalJ Biol Chem
PubMed ID11349124
'The human CYP11A1 gene is expressed specifically in steroidogenic tissues and encodes cytochrome P450scc, which catalyzes the first step in steroid synthesis. A region of the 5'-flanking DNA of the gene from nucleotides -155 to -131 (-155/-131) is shown to activate transcription in steroidogenic human placental JEG-3 (1) and adrenal ... More
3'-end fluorochromized and haptenized oligonucleotides as in situ hybridization probes for multiple, simultaneous RNA detection.
AuthorsDirks RW, Van Gijlswijk RP, Vooijs MA, Smit AB, Bogerd J, van Minnen J, Raap AK, Van der Ploeg M
JournalExp Cell Res
PubMed ID2026182
'We have used fluorescein-, digoxigenin- and biotin-(di)deoxyXTPs and terminal deoxynucleotidyl transferase for small scale labeling of synthetic oligonucleotide probes and here we show the applicability of such probes for the in situ detection of multiple RNA sequences. The enzymatic 3''-end-labeling methods proved to be good alternatives for the chemical fluorochrome ... More
The contribution of bone marrow-derived cells to the development of renal interstitial fibrosis.
'Recent evidence suggests that bone marrow (BM)-derived cells may integrate into the kidney, giving rise to functional renal cell types, including endothelial and epithelial cells and myofibroblasts. BM-derived cells can contribute to repair of the renal peritubular capillary (PTC) network following acute ischemic injury. However, the cell fate and regulation ... More
'Pseudogout is an autoinflammatory condition triggered by calcium pyrophosphate dehydrate (CPPD) crystal deposition in the joints. The innate immune system is irritated by and responds to the presence of the crystals with an inflammatory response. The synovial fluid contains activated inflammatory macrophages and neutrophil granulocytes. Several details of crystal-induced macrophage ... More
Zygotic expression of the pebble locus is required for cytokinesis during the postblastoderm mitoses of Drosophila.
AuthorsHime G, Saint R
JournalDevelopment
PubMed ID1576956
'Mutations at the pebble locus of Drosophila melanogaster result in embryonic lethality. Examination of homozygous mutant embryos at the end of embryogenesis revealed the presence of fewer and larger cells which contained enlarged nuclei. Characterization of the embryonic cell cycles using DAPI, propidium iodide, anti-tubulin and anti-spectrin staining showed that ... More
Loss of PINK1 function promotes mitophagy through effects on oxidative stress and mitochondrial fission.
AuthorsDagda RK, Cherra SJ, Kulich SM, Tandon A, Park D, Chu CT,
JournalJ Biol Chem
PubMed ID19279012
'Mitochondrial dysregulation is strongly implicated in Parkinson disease. Mutations in PTEN-induced kinase 1 (PINK1) are associated with familial parkinsonism and neuropsychiatric disorders. Although overexpressed PINK1 is neuroprotective, less is known about neuronal responses to loss of PINK1 function. We found that stable knockdown of PINK1 induced mitochondrial fragmentation and autophagy ... More
Adenovirus E4 open reading frame 4-induced apoptosis involves dysregulation of Src family kinases.
AuthorsLavoie JN, Champagne C, Gingras MC, Robert A
JournalJ Cell Biol
PubMed ID10973994
'The adenoviral early region 4 open reading frame 4 (E4orf4) death factor induces p53-independent apoptosis in many cell types and appears to kill selectively transformed cells. Here we show that expression of E4orf4 in transformed epithelial cells results in early caspase-independent membrane blebbing, associated with changes in the organization of ... More
Mps1 is a kinetochore-associated kinase essential for the vertebrate mitotic checkpoint.
AuthorsAbrieu A, Magnaghi-Jaulin L, Kahana JA, Peter M, Castro A, Vigneron S, Lorca T, Cleveland DW, Labbé JC
JournalCell
PubMed ID11461704
'The mitotic checkpoint acts to inhibit entry into anaphase until all chromosomes have successfully attached to spindle microtubules. Unattached kinetochores are believed to release an activated form of Mad2 that inhibits APC/C-dependent ubiquitination and subsequent proteolysis of components needed for anaphase onset. Using Xenopus egg extracts, a vertebrate homolog of ... More
Systems survey of endocytosis by multiparametric image analysis.
AuthorsCollinet C, Stöter M, Bradshaw CR, Samusik N, Rink JC, Kenski D, Habermann B, Buchholz F, Henschel R, Mueller MS, Nagel WE, Fava E, Kalaidzidis Y, Zerial M,
JournalNature
PubMed ID20190736
'Endocytosis is a complex process fulfilling many cellular and developmental functions. Understanding how it is regulated and integrated with other cellular processes requires a comprehensive analysis of its molecular constituents and general design principles. Here, we developed a new strategy to phenotypically profile the human genome with respect to transferrin ... More
Nuclear import of the ran exchange factor, RCC1, is mediated by at least two distinct mechanisms.
AuthorsNemergut ME, Macara IG
JournalJ Cell Biol
PubMed ID10811825
'RCC1, the only known guanine-nucleotide exchange factor for the Ran GTPase, is an approximately 45-kD nuclear protein that can bind chromatin. An important question concerns how RCC1 traverses the nuclear envelope. We now show that nuclear RCC1 is not exported readily in interphase cells and that the import of RCC1 ... More
Pepsin pretreatment allows collagen IV immunostaining of blood vessels in adult mouse brain.
AuthorsFranciosi S, De Gasperi R, Dickstein DL, English DF, Rocher AB, Janssen WG, Christoffel D, Sosa MA, Hof PR, Buxbaum JD, Elder GA
JournalJ Neurosci Methods
PubMed ID17403541
'While the brain vasculature can be imaged with many methods, immunohistochemistry has distinct advantages due to its simplicity and applicability to archival tissue. However, immunohistochemical staining of the murine brain vasculature in aldehyde fixed tissue has proven elusive and inconsistent using current protocols. Here we investigated whether antigen retrieval methods ... More
UGO1 encodes an outer membrane protein required for mitochondrial fusion.
AuthorsSesaki H, Jensen RE
JournalJ Cell Biol
PubMed ID11257114
'Membrane fusion plays an important role in controlling the shape, number, and distribution of mitochondria. In the yeast Saccharomyces cerevisiae, the outer membrane protein Fzo1p has been shown to mediate mitochondrial fusion. Using a novel genetic screen, we have isolated new mutants defective in the fusion of their mitochondria. One ... More
Chondrocytes utilize a cholesterol-dependent lipid translocator to externalize phosphatidylserine.
AuthorsDamek-Poprawa M, Golub E, Otis L, Harrison G, Phillips C, Boesze-Battaglia K
JournalBiochemistry
PubMed ID16519527
'During endochondral ossification, growth plate chondrocytes release plasma membrane (PM) derived matrix vesicles (MV), which are the site of initial hydroxyapatite crystal formation. MV constituents which facilitate the mineralization process include the integral membrane ectoenzymes alkaline phosphatase (ALPase) and nucleotide pyrophosphatase phosphodiesterase (NPP1/PC-1), along with a phosphatidylserine- (PS-) rich membrane ... More
Regulated localization of Rab18 to lipid droplets: effects of lipolytic stimulation and inhibition of lipid droplet catabolism.
AuthorsMartin S, Driessen K, Nixon SJ, Zerial M, Parton RG
JournalJ Biol Chem
PubMed ID16207721
'Rab GTPases are crucial regulators of membrane traffic. Here we have examined a possible association of Rab proteins with lipid droplets (LDs), neutral lipid-containing organelles surrounded by a phospholipid monolayer, also known as lipid bodies, which have been traditionally considered relatively inert storage organelles. Although we found close apposition between ... More
A kinase-independent function of Ask1 in caspase-independent cell death.
AuthorsCharette SJ, Lambert H, Landry J
JournalJ Biol Chem
PubMed ID11493600
'Ask1 (apoptosis signal-regulating kinase 1) is activated as a consequence of cell exposure to a variety of stresses and can then initiate apoptosis. A known pathway of apoptosis downstream of Ask1 involves the activation of the stress-activated protein kinases, the release of cytochrome c from mitochondria, the activation of caspases, ... More
Tumor promoter arsenite stimulates histone H3 phosphoacetylation of proto-oncogenes c-fos and c-jun chromatin in human diploid fibroblasts.
AuthorsLi J, Gorospe M, Barnes J, Liu Y
JournalJ Biol Chem
PubMed ID12547826
'Although epidemiological studies have long established that inorganic arsenic is a potent human carcinogen, the underlying mechanisms are still poorly understood. Recent studies suggest that inorganic arsenic may act as a tumor promoter by perturbing key signaling transduction pathways. We have shown previously that arsenite can potently activate the mitogen-activated ... More
Monomethylation of histone H4-lysine 20 is involved in chromosome structure and stability and is essential for mouse development.
AuthorsOda H, Okamoto I, Murphy N, Chu J, Price SM, Shen MM, Torres-Padilla ME, Heard E, Reinberg D,
JournalMol Cell Biol
PubMed ID19223465
'PR-Set7/Set8/KMT5A is the sole enzyme known to catalyze monomethylation of histone H4 lysine 20 (H4K20) and is present only in multicellular organisms that compact a large fraction of their DNA. We found that mouse embryos that are homozygous null mutants for the gene PR-Set7 display early embryonic lethality prior to ... More
'The generation of micronuclei is a reflection of DNA damage, defective mitosis, and loss of genetic material. The involvement of the MAPK pathway in mediating v-ras-induced micronuclei in NIH 3T3 cells was examined by inhibiting MAPK activation. Conversely, the MAPK pathway was constitutively activated by infecting cells with a v-mos ... More
Regulation of cytokine-independent survival kinase (CISK) by the Phox homology domain and phosphoinositides.
AuthorsXu J, Liu D, Gill G, Songyang Z
JournalJ Cell Biol
PubMed ID11514587
'PKB/Akt and serum and glucocorticoid-regulated kinase (SGK) family kinases are important downstream targets of phosphatidylinositol 3 (PI-3) kinase and have been shown to mediate a variety of cellular processes, including cell growth and survival. Although regulation of Akt can be achieved through several mechanisms, including its phosphoinositide-binding Pleckstrin homology (PH) ... More
Sensitive multicolor fluorescence in situ hybridization using catalyzed reporter deposition (CARD) amplification.
AuthorsSpeel EJ, Ramaekers FC, Hopman AH
JournalJ Histochem Cytochem
PubMed ID9313806
'We describe the simultaneous localization of DNA sequences in cell and chromosome preparations by means of differently fluorochrome-labeled (AMCA, FITC, TRITC) tyramides using the catalyzed reporter deposition (CARD) procedure. For this purpose, repeated as well as single-copy DNA probes were labeled with biotin, digoxigenin, and FITC, hybridized, and visualized with ... More
Sid4p is required to localize components of the septation initiation pathway to the spindle pole body in fission yeast.
AuthorsChang L, Gould KL
JournalProc Natl Acad Sci U S A
PubMed ID10805785
'A mutation in the Schizosaccharomyces pombe sid4(+) (septation initiation defective) gene was isolated in a screen for mutants defective in cytokinesis. We have cloned sid4(+) and have found that sid4(+) encodes a previously unknown 76.4-kDa protein that localizes to the spindle pole body (SPB) throughout the cell cycle. Sid4p is ... More
Androgen induces expression of the multidrug resistance protein gene MRP4 in prostate cancer cells.
AuthorsCai C, Omwancha J, Hsieh CL, Shemshedini L
JournalProstate Cancer Prostatic Dis
PubMed ID17003774
'Multidrug resistance-associated proteins (MRPs) may mediate multidrug resistance in tumor cells. Using a gene array analysis, we have identified MRP4 as an androgen receptor (AR)-regulated gene. Dihydrotestosterone induced MRP4 expression in both androgen-dependent and -independent LNCaP cells, whereas there was little detectable expression in PC-3 or normal prostate epithelial cells. ... More
An in vivo membrane fusion assay implicates SpoIIIE in the final stages of engulfment during Bacillus subtilis sporulation.
AuthorsSharp MD, Pogliano K
JournalProc Natl Acad Sci U S A
PubMed ID10588743
'Shortly after the synthesis of the two cells required for sporulation in Bacillus subtilis, the membranes of the larger mother cell begin to migrate around and engulf the smaller forespore cell. At the completion of this process the leading edges of the migrating membrane meet and fuse, releasing the forespore ... More
Mps3p is a novel component of the yeast spindle pole body that interacts with the yeast centrin homologue Cdc31p.
AuthorsJaspersen SL, Giddings TH, Winey M
JournalJ Cell Biol
PubMed ID12486115
'Accurate duplication of the Saccharomyces cerevisiae spindle pole body (SPB) is required for formation of a bipolar mitotic spindle. We identified mutants in SPB assembly by screening a temperature-sensitive collection of yeast for defects in SPB incorporation of a fluorescently marked integral SPB component, Spc42p. One SPB assembly mutant contained ... More
Microtubule-associated movement of mitochondria and small particles in Acanthamoeba castellanii.
AuthorsBaumann O, Murphy DB
JournalCell Motil Cytoskeleton
PubMed ID8608609
'Using video-enhanced differential interference microscopy and digital image processing, we have observed organelle motility in Acanthamoeba castellanii. In amoebae taken from cultures in rapid growth phase, mitochondria and small particles moved over distances of several microns and at an average velocity of approximately 2 microns/s. Mitochondrial motility was verified by ... More
Optimized fixation and immunofluorescence staining methods for Dictyostelium cells.
AuthorsHagedorn M, Neuhaus EM, Soldati T,
JournalMethods Mol Biol
PubMed ID16957300
'Recent years have seen a powerful revival of fluorescence microscopy techniques, both to observe live cells and fixed objects. The limits of sensitivity, simultaneous detection of multiple chromophores, and spatial resolution have all been pushed to the extreme. Therefore, it is essential to improve in parallel the quality of the ... More
Bcl-G, a novel pro-apoptotic member of the Bcl-2 family.
AuthorsGuo B, Godzik A, Reed JC
JournalJ Biol Chem
PubMed ID11054413
'A new member of the Bcl-2 family was identified, Bcl-G. The human BCL-G gene consists of 6 exons, resides on chromosome 12p12, and encodes two proteins through alternative mRNA splicing, Bcl-G(L) (long) and Bcl-G(S) (short) consisting of 327 and 252 amino acids in length, respectively. Bcl-G(L) and Bcl-G(S) have identical ... More
PAK-family kinases regulate cell and actin polarization throughout the cell cycle of Saccharomyces cerevisiae.
AuthorsHolly SP, Blumer KJ
JournalJ Cell Biol
PubMed ID10562285
'During the cell cycle of the yeast Saccharomyces cerevisiae, the actin cytoskeleton and cell surface growth are polarized, mediating bud emergence, bud growth, and cytokinesis. We have determined whether p21-activated kinase (PAK)-family kinases regulate cell and actin polarization at one or several points during the yeast cell cycle. Inactivation of ... More
Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells.
AuthorsHaarer BK, Lillie SH, Adams AE, Magdolen V, Bandlow W, Brown SS
JournalJ Cell Biol
PubMed ID2404021
'We have isolated profilin from yeast (Saccharomyces cerevisiae) and have microsequenced a portion of the protein to confirm its identity; the region microsequenced agrees with the predicted amino acid sequence from a profilin gene recently isolated from S. cerevisiae (Magdolen, V., U. Oechsner, G. Müller, and W. Bandlow. 1988. Mol. ... More
E-cadherin is the receptor for internalin, a surface protein required for entry of L. monocytogenes into epithelial cells.
AuthorsMengaud J, Ohayon H, Gounon P, Mege R-M Cossart P
JournalCell
PubMed ID8601315
'We report the first identification of a cellular receptor mediating entry of a gram-positive bacterium into nonphagocytotic cells. By an affinity chromatography approach, we identified E-cadherin as the ligand for internalin, an L. monocytogenes protein essential for entry into epithelial cells. Expression of the chicken homolog of E-cadherin (L-CAM) in ... More
Chicken coagulation factor XIIIA is produced by the theca externa and stabilizes the ovarian follicular wall.
AuthorsRecheis B, Osanger A, Haubenwallner S, Schneider WJ, Nimpf J
JournalJ Biol Chem
PubMed ID10948196
'Development of the follicle in egg-laying species such as the chicken is regulated by systemic factors as well as by the highly orchestrated interplay of differentially expressed genes within this organ. Differential mRNA display analysis of defined phases of follicle development resulted in the characterization of coagulation factor XIIIA. It ... More
High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones.
'Cosmid clones containing human DNA inserts have been mapped on chromosome 11 by fluorescence in situ hybridization under conditions that suppress signal from repetitive DNA sequences. Thirteen known genes, one chromosome 11-specific DNA repeat, and 36 random clones were analyzed. High-resolution mapping was facilitated by using digital imaging microscopy and ... More
An actin-binding protein of the Sla2/Huntingtin interacting protein 1 family is a novel component of clathrin-coated pits and vesicles.
AuthorsEngqvist-Goldstein AE, Kessels MM, Chopra VS, Hayden MR, Drubin DG
JournalJ Cell Biol
PubMed ID10613908
'The actin cytoskeleton has been implicated in endocytosis, yet few molecules that link these systems have been identified. Here, we have cloned and characterized mHip1R, a protein that is closely related to huntingtin interacting protein 1 (Hip1). These two proteins are mammalian homologues of Sla2p, an actin binding protein important ... More
The nucleolus exhibits an osmotically regulated gatekeeping activity that controls the spatial dynamics and functions of nucleolin.
AuthorsYang L, Reece JM, Cho J, Bortner CD, Shears SB,
JournalJ Biol Chem
PubMed ID18299322
'We demonstrate that physiologically relevant perturbations in the osmotic environment rheostatically regulate a gatekeeping function for the nucleolus that controls the spatial dynamics and functions of nucleolin. HeLa cells and U2-OS osteosarcoma cells were osmotically challenged with 100-200 mm sorbitol, and the intranuclear distribution of nucleolin was monitored by confocal ... More
HEAT repeats mediate plasma membrane localization of Tor2p in yeast.
AuthorsKunz J, Schneider U, Howald I, Schmidt A, Hall MN
JournalJ Biol Chem
PubMed ID10973982
'The subcellular distribution of Tor1p and Tor2p, two phosphatidylinositol kinase homologs and targets of the immunosuppressive drug rapamycin in Saccharomyces cerevisiae, was analyzed. We found that Tor protein is peripherally associated with membranes. Subcellular fractionation and immunofluorescence studies showed that Tor1p and Tor2p associate with the plasma membrane and a ... More
Slit proteins prevent midline crossing and determine the dorsoventral position of major axonal pathways in the mammalian forebrain.
AuthorsBagri A, Marín O, Plump AS, Mak J, Pleasure SJ, Rubenstein JL, Tessier-Lavigne M
JournalNeuron
PubMed ID11804571
'We report that Slit proteins, a family of secreted chemorepellents, are crucial for the proper development of several major forebrain tracts. Mice deficient in Slit2 and, even more so, mice deficient in both Slit1 and Slit2 show significant axon guidance errors in a variety of pathways, including corticofugal, callosal, and ... More
Fluorescence in situ hybridization using horseradish peroxidase-labeled oligodeoxynucleotides and tyramide signal amplification for sensitive DNA and mRNA detection.
Authorsvan de Corput MP, Dirks RW, van Gijlswijk RP, van de Rijke FM, Raap AK
JournalHistochem Cell Biol
PubMed ID9792422
'We have used horseradish peroxidase-labeled 40 mer oligodeoxynucleotides (HRP-ODNs) specific for the human cytomegalovirus immediate early gene (HCMV-IE) and a novel dinitrophenol-tyramide signal amplification reagent (DNP-TSA plus) to evaluate their utility in fluorescence in situ hybridization (FISH). For DNA FISH, single or cocktails of HRP-ODNs were hybridized to metaphase chromosomes ... More
Paracrine signaling through the JAK/STAT pathway activates invasive behavior of ovarian epithelial cells in Drosophila.
AuthorsSilver DL, Montell DJ
JournalCell
PubMed ID11779460
'The JAK/STAT signaling pathway, renowned for its effects on cell proliferation and survival, is constitutively active in various human cancers, including ovarian. We have found that JAK and STAT are required to convert the border cells in the Drosophila ovary from stationary, epithelial cells to migratory, invasive cells. The ligand ... More
Subcellular localization and membrane topology of the Dengue virus type 2 Non-structural protein 4B.
AuthorsMiller S, Sparacio S, Bartenschlager R
JournalJ Biol Chem
PubMed ID16436383
'Dengue virus (DV) is a member of the family Flaviviridae. These positive strand RNA viruses encode a polyprotein that is processed in case of DV into 10 proteins. Although for most of these proteins distinct functions have been defined, this is less clear for the highly hydrophobic non-structural protein (NS) ... More
Focal adhesion size controls tension-dependent recruitment of alpha-smooth muscle actin to stress fibers.
AuthorsGoffin JM, Pittet P, Csucs G, Lussi JW, Meister JJ, Hinz B
JournalJ Cell Biol
PubMed ID16401722
'Expression of alpha-smooth muscle actin (alpha-SMA) renders fibroblasts highly contractile and hallmarks myofibroblast differentiation. We identify alpha-SMA as a mechanosensitive protein that is recruited to stress fibers under high tension. Generation of this threshold tension requires the anchoring of stress fibers at sites of 8-30-microm-long "supermature" focal adhesions (suFAs), which ... More
Activation of nuclear factor-kappaB is necessary for myotrophin-induced cardiac hypertrophy.
AuthorsGupta S, Purcell NH, Lin A, Sen S
JournalJ Cell Biol
PubMed ID12486112
'The transcription factor nuclear factor-kappaB (NF-kappaB) regulates expression of a variety of genes involved in immune responses, inflammation, proliferation, and programmed cell death (apoptosis). Here, we show that in rat neonatal ventricular cardiomyocytes, activation of NF-kappaB is involved in the hypertrophic response induced by myotrophin, a hypertrophic activator identified from ... More
Identification of ribonucleotide reductase protein R1 as an activator of microtubule nucleation in Xenopus egg mitotic extracts.
AuthorsTakada S, Shibata T, Hiraoka Y, Masuda H
JournalMol Biol Cell
PubMed ID11102516
'Microtubule nucleation on the centrosome and the fungal equivalent, the spindle pole body (SPB), is activated at the onset of mitosis. We previously reported that mitotic extracts prepared from Xenopus unfertilized eggs convert the interphase SPB of fission yeast into a competent state for microtubule nucleation. In this study, we ... More
Head and/or CaaX domain deletions of lamin proteins disrupt preformed lamin A and C but not lamin B structure in mammalian cells.
AuthorsIzumi M, Vaughan OA, Hutchison CJ, Gilbert DM
JournalMol Biol Cell
PubMed ID11102526
'The nuclear lamina is an important determinant of nuclear architecture. Mutations in A-type but not B-type lamins cause a range of human genetic disorders, including muscular dystrophy. Dominant mutations in nuclear lamin proteins have been shown to disrupt a preformed lamina structure in Xenopus egg extracts. Here, a series of ... More
Expression and partial characterization of kinesin-related proteins in differentiating and adult skeletal muscle.
AuthorsGinkel LM, Wordeman L
JournalMol Biol Cell
PubMed ID11102514
'Using pan-kinesin antibodies to screen a differentiating C2C12 cell library, we identified the kinesin proteins KIF3A, KIF3B, and conventional kinesin heavy chain to be present in differentiating skeletal muscle. We compared the expression and subcellular localization characteristics of these kinesins in myogenic cells to others previously identified in muscle, neuronal, ... More
Domains of axin involved in protein-protein interactions, Wnt pathway inhibition, and intracellular localization.
AuthorsFagotto F, Jho E, Zeng L, Kurth T, Joos T, Kaufmann C, Costantini F
JournalJ Cell Biol
PubMed ID10330403
'Axin was identified as a regulator of embryonic axis induction in vertebrates that inhibits the Wnt signal transduction pathway. Epistasis experiments in frog embryos indicated that Axin functioned downstream of glycogen synthase kinase 3beta (GSK3beta) and upstream of beta-catenin, and subsequent studies showed that Axin is part of a complex ... More
Fluorescence in situ hybridization (FISH): DNA probe production and hybridization criteria.
AuthorsNath J, Johnson KL
JournalBiotech Histochem
PubMed ID9554580
'We describe methods for the production of fluorescence in situ hybridization (FISH) probes and the utilization of these probes for the detection of complementary DNA sequences with accuracy and sensitivity for application in both basic research and clinical diagnosis. Due to the frequent use of FISH in many laboratories, it ... More
Estimating relative carbonyl levels in muscle microstructures by fluorescence imaging.
AuthorsFeng J, Navratil M, Thompson LV, Arriaga EA,
JournalAnal Bioanal Chem
PubMed ID18548236
'The increase in the levels of protein carbonyls, biomarkers of oxidative stress, appears to play an important role in aging skeletal muscle. However, the exact distributions of carbonyls among various skeletal muscle microstructures still remain largely unknown, partly owing to the lack of adequate techniques to carry out these measurements. ... More
Aggresomes resemble sites specialized for virus assembly.
AuthorsHeath CM, Windsor M, Wileman T
JournalJ Cell Biol
PubMed ID11331297
'The large cytoplasmic DNA viruses such as poxviruses, iridoviruses, and African swine fever virus (ASFV) assemble in discrete perinuclear foci called viral factories. Factories exclude host proteins, suggesting that they are novel subcellular structures induced by viruses. Novel perinuclear structures, called aggresomes are also formed by cells in response to ... More
Identification and characterization of a novel Golgi protein, golgin-67.
AuthorsJakymiw A, Raharjo E, Rattner JB, Eystathioy T, Chan EK, Fujita DJ
JournalJ Biol Chem
PubMed ID10660574
'In the course of screening a lambdagt11 human leukemic T-cell cDNA expression library with an antibody specific to the mitotic target of Src, Sam68, we identified and cloned a cDNA encoding a novel protein with a predicted molecular mass of 51.4 kDa. Polyclonal antibodies raised to a His(6)-tagged construct of ... More
Promyelocytic leukemia (PML) nuclear bodies are protein structures that do not accumulate RNA.
AuthorsBoisvert FM, Hendzel MJ, Bazett-Jones DP
JournalJ Cell Biol
PubMed ID10648561
'The promyelocytic leukemia (PML) nuclear body (also referred to as ND10, POD, and Kr body) is involved in oncogenesis and viral infection. This subnuclear domain has been reported to be rich in RNA and a site of nascent RNA synthesis, implicating its direct involvement in the regulation of gene expression. ... More
NFAT: ubiquitous regulator of cell differentiation and adaptation.
AuthorsHorsley V, Pavlath GK
JournalJ Cell Biol
PubMed ID11877454
'The nuclear factor of activated T cells (NFAT) proteins are a family of transcription factors whose activation is controlled by calcineurin, a Ca(2+)-dependent phosphatase. Originally identified in T cells as inducers of cytokine gene expression, NFAT proteins play varied roles in cells outside of the immune system. This review addresses ... More
Retinoic acid regulates the expression of photoreceptor transcription factor NRL.
AuthorsKhanna H, Akimoto M, Siffroi-Fernandez S, Friedman JS, Hicks D, Swaroop A
JournalJ Biol Chem
PubMed ID16854989
'NRL (neural retina leucine zipper) is a key basic motif-leucine zipper (bZIP) transcription factor, which orchestrates rod photoreceptor differentiation by activating the expression of rod-specific genes. The deletion of Nrl in mice results in functional cones that are derived from rod precursors. However, signaling pathways modulating the expression or activity ... More
The C2A domain of double C2 protein gamma contains a functional nuclear localization signal.
AuthorsFukuda M, Saegusa C, Kanno E, Mikoshiba K
JournalJ Biol Chem
PubMed ID11371549
'The C2 domain was originally defined as a homologous domain to the C2 regulatory region of Ca2+ -dependent protein kinase C and has been identified in more than 50 different signaling molecules. The original C2 domain of protein kinase Calpha functions as a Ca2+ binding module, and the Ca2+ binding ... More
Caspase-mediated cleavage of the stacking protein GRASP65 is required for Golgi fragmentation during apoptosis.
AuthorsLane JD, Lucocq J, Pryde J, Barr FA, Woodman PG, Allan VJ, Lowe M
JournalJ Cell Biol
PubMed ID11815631
'The mammalian Golgi complex is comprised of a ribbon of stacked cisternal membranes often located in the pericentriolar region of the cell. Here, we report that during apoptosis the Golgi ribbon is fragmented into dispersed clusters of tubulo-vesicular membranes. We have found that fragmentation is caspase dependent and identified GRASP65 ... More