A high-throughput fluorescent polarization assay for nuclear receptor binding utilizing crude receptor extract.
Authors:Lin S, Bock CL, Gardner DB, Webster JC, Favata MF, Trzaskos JM, Oldenburg KR
Journal:Anal Biochem
PubMed ID:11743686
'A homogenous high-throughput assay has been developed to measure the binding between nuclear receptors and test compounds. This assay applies a fluorescence polarization (FP) detection method using human glucocorticoid receptor (GR) as a model system. Crude receptor extract, which requires no additional purification, is used in the assay. The binding ... More