Measurement of platelet-activating factor acetylhydrolase activity by quantitative high-performance liquid chromatography determination of coumarin-derivatized 1-O-alkyl-2-sn-lysoglyceryl-3-phosphorylcholine.
AuthorsBalestrieri C, Camussi G, Giovane A, Iorio EL, Quagliuolo L, Servillo L
JournalAnal Biochem
PubMed ID8789711
A sensitive method for determining platelet-activating factor acetylhydrolase (PAF-AH) activity in human serum, using high-performance liquid chromatography (HPLC) with a fluorimetric detection, is described. The method is based on the derivatization with 7-diethylaminocoumarin-3-carbonylazide of the 2-lyso-PAF, by-product of PAF-AH activity, extracted from the reaction mixture by phase partition into organic ... More
Interaction of tubulin with a new fluorescent analogue of vinblastine.
Vinblastine is an antimitotic agent that has been used extensively in cancer chemotherapy. The biological effects of the drug are believed to be the result of its interaction with tubulin, the major component of cellular microtubules. Fluorescence spectroscopy is a powerful and versatile technique for studying drug-tubulin interactions, but it ... More
Alterations in serum phosphatidylcholine fatty acyl species by eicosapentaenoic and docosahexaenoic ethyl esters in patients with severe hypertriglyceridemia.
AuthorsMcKeone BJ, Osmundsen K, Brauchi D, Pao Q, Payton-Ross C, Kilinç C, Kummerow FA, Pownall HJ
JournalJ Lipid Res
PubMed ID9101424
A new and sensitive method has been developed to analyze the molecular species of glycerophospholipids. This method was used to examine the effects of hypolipidemic intervention with n-3 fatty acids on the serum phosphatidylcholine species in severely hypertriglyceridemic patients. The drug treated group (n = 19) received 4 g/day of ... More