Direct measurement of endosomal pH in living cells of the rat yolk sac epithelium by laser confocal microscopy.
AuthorsIchimura T, Hatae T, Ishida T
JournalEur J Cell Biol
PubMed ID9309389
'Endocytosis of dual-fluorescent dextran by epithelial cells was observed in the perfused rat yolk sac using a confocal laser scanning microscope. Endosomal pH was quantified from confocal images using a dual-fluorescence ratiometry technique. Changes in endosomal pH were followed over time up to 32 min with and without Brefeldin A ... More
Phagocytic and macropinocytic activity in MARCKS-deficient macrophages and fibroblasts.
AuthorsCarballo E, Pitterle DM, Stumpo DJ, Sperling RT, Blackshear PJ
JournalAm J Physiol
PubMed ID10409119
Macrophages express high levels of the myristoylated, alanine-rich, C kinase substrate (MARCKS), an actin cross-linking protein. To investigate a possible role of MARCKS in macrophage function, fetal liver-derived macrophages were generated from wild-type and MARCKS knockout mouse embryos. No differences between the wild-type and MARCKS-deficient macrophages with respect to morphology ... More
Biochemical and functional characterization of intercellular adhesion and gap junctions in fibroblasts.
AuthorsKo K, Arora P, Lee W, McCulloch C
JournalAm J Physiol Cell Physiol
PubMed ID10898726
Despite their significance in wound healing, little is known about the molecular determinants of cell-to-cell adhesion and gap junctional communication in fibroblasts. We characterized intercellular adherens junctions and gap junctions in human gingival fibroblasts (HGFs) using a novel model. Calcein-labeled donor cells in suspension were added onto an established, Texas ... More
A long-lived fusogenic state is induced in erythrocyte ghosts by electric pulses.
AuthorsSowers AE
JournalJ Cell Biol
PubMed ID3958050
Treatment of erythrocyte ghosts in random positions in a suspension with membrane fusion-inducing direct current electric field pulses causes the membranes to become fusogenic. Significant fusion yields are observed if the membranes are dielectrophoretically aligned into membrane-membrane contact with a weak alternating electric field as much as 5 min after ... More
Fluorescent markers to study membrane retrieval in antidiuretic hormone-treated toad urinary bladder.
AuthorsHarris HW, Wade JB, Handler JS
JournalAm J Physiol
PubMed ID2426962
Antidiuretic hormone (ADH) stimulation of toad urinary bladder causes fusion of intracellular vesicles called aggrephores with the apical plasma membrane of granular cells. Aggrephores contain intramembrane particle aggregates whose appearance in the apical membrane is believed to produce a large increase in its water permeability. ADH removal (ADH washout) is ... More