The CRE/CREB pathway is transiently expressed in thalamic circuit development and contributes to refinement of retinogeniculate axons.
AuthorsPham TA, Rubenstein JL, Silva AJ, Storm DR, Stryker MP
JournalNeuron
PubMed ID11516398
'The development of precise connections in the mammalian brain proceeds through refinement of initially diffuse patterns, a process that occurs largely within critical developmental windows. To elucidate the molecular pathways that orchestrate these early periods of circuit remodeling, we have examined the role of a calcium- and cAMP-regulated transcriptional pathway. ... More
Anatomical and physiological localization of prelabeled grafts in rat hippocampus.
AuthorsPyapali GK, Turner DA, Madison RD
JournalExp Neurol
PubMed ID1577121
'Dissociated rat fetal hippocampal cells were grafted into normal adult rats. The fetal cells were incubated with one of a number of fluorescent compounds at the time of the dissociation to facilitate identification of the individual grafted cells. The fluorescent labels which were analyzed for this purpose included rhodamine latex ... More
Neural tube closure in Xenopus laevis involves medial migration, directed protrusive activity, cell intercalation and convergent extension.
AuthorsDavidson LA, Keller RE
JournalDevelopment
PubMed ID10498689
'We have characterized the cell movements and prospective cell identities as neural folds fuse during neural tube formation in Xenopus laevis. A newly developed whole-mount, two-color fluorescent RNA in situ hybridization method, visualized with confocal microscopy, shows that the dorsal neural tube gene xpax3 and the neural-crest-specific gene xslug are ... More
Origin, course, and laterality of spinocerebellar axons in the North American opossum, Didelphis virginiana.
AuthorsTerman JR, Wang XM, Martin GF
JournalAnat Rec
PubMed ID9713988
'Spinocerebellar axons have been studied extensively in placental mammals, but there have been no full reports on their origin, laterality, or spinal course in any marsupial. We have used the North American opossum (Didelphis virginiana) to obtain such information and to ask whether any spinocerebellar neurons innervate both the anterior ... More
Number, distribution, and morphology of retinal ganglion cells with axons regenerated into peripheral nerve graft in adult cats.
AuthorsWatanabe M, Sawai H, Fukuda Y
JournalJ Neurosci
PubMed ID8478691
'The optic nerve of adult cats was transected and an autologous peripheral nerve was transplanted to the stump. Two months later retinal ganglion cells (RGCs) that had regenerated their axons were retrogradely labeled with fluorescent dye injected into the graft. The more than 4000 labeled cells, which were 3.0-4.3% of ... More
Molecular and functional characterization of inositol trisphosphate receptors during early zebrafish development.
AuthorsAshworth R, Devogelaere B, Fabes J, Tunwell RE, Koh KR, De Smedt H, Patel S
JournalJ Biol Chem
PubMed ID17331947
'Fluctuations in cytosolic Ca(2+) are crucial for a variety of cellular processes including many aspects of development. Mobilization of intracellular Ca(2+) stores via the production of inositol trisphosphate (IP(3)) and the consequent activation of IP(3)-sensitive Ca(2+) channels is a ubiquitous means by which diverse stimuli mediate their cellular effects. Although ... More
PI3P signaling regulates receptor sorting but not transport in the endosomal pathway.
AuthorsPetiot A, Faure J, Stenmark H, Gruenberg J
JournalJ Cell Biol
PubMed ID12975344
'While evidence is accumulating that phosphoinositide signaling plays a crucial role in growth factor and hormone receptor down-regulation, this signaling pathway has also been proposed to regulate endosomal membrane transport and multivesicular endosome biogenesis. Here, we have followed the fate of the down-regulated EGF receptor (EGFR) and bulk transport (fluid ... More
Dermal microvascular endothelial cells express the 180-kDa macrophage mannose receptor in situ and in vitro.
AuthorsGröger M, Holnthoner W, Maurer D, Lechleitner S, Wolff K, Mayr BB, Lubitz W, Petzelbauer P
JournalJ Immunol
PubMed ID11067894
'Expression of the 180-kDa mannose receptor (MR) is mainly found on cells of the macrophage lineage. MR mediates the uptake of micro-organisms and host-derived glycoproteins. We demonstrate that endothelium of the human skin in situ and dermal microvascular endothelial cells (DMEC) in vitro expressed MR at both the protein and ... More
Vital staining of the hearing organ: visualization of cellular structure with confocal microscopy.
AuthorsFlock A, Scarfone E, Ulfendahl M
JournalNeuroscience
PubMed ID9466411
'Cells inside the intact organ of Corti were labelled with fluorescent probes reflecting various aspects of structure and function. The dyes were introduced into the perilymphatic space by perfusion of the scala tympani of the temporal bone from the guinea-pig maintained in isolation. The dyes were able to diffuse through ... More
A localized elevation of cytosolic free calcium is associated with cytokinesis in the zebrafish embryo.
AuthorsChang DC, Meng C
JournalJ Cell Biol
PubMed ID8522610
'Cytokinesis, a key step in cell division, is known to be precisely regulated both in its timing and location. At present, the regulatory mechanism of cytokinesis is not well understood, although it has been suggested that calcium signaling may play an important role in this process. To test this notion, ... More
Uptake and trafficking of fluorescent conjugates of folic acid in intact kidney determined using intravital two-photon microscopy.
AuthorsSandoval RM, Kennedy MD, Low PS, Molitoris BA
JournalAm J Physiol Cell Physiol
PubMed ID15102609
'Intravital two-photon microscopy was used to follow the uptake and trafficking of fluorescent conjugates of folic acid in the rat kidney. Intravenously administered folate-linked dye molecules quickly filled the plasma volume but not cellular components of the blood. Glomerular filtration occurred immediately and binding to proximal tubule cells was seen ... More
Fluorescent labeling of dissociated fetal cells for tissue culture.
AuthorsParamore CG, Turner DA, Madison RD
JournalJ Neurosci Methods
PubMed ID1434752
'The ability to pre-label cells used in transplantation experiments would have the potential benefits of identification of cell type and associated processes and the analysis of graft migration in the host. We have used an in vitro tissue culture system as a model to test several fluorescent dyes for this ... More
Golgi spectrin: identification of an erythroid beta-spectrin homolog associated with the Golgi complex.
AuthorsBeck KA, Buchanan JA, Malhotra V, Nelson WJ
JournalJ Cell Biol
PubMed ID7962054
'Spectrin is a major component of a membrane-associated cytoskeleton involved in the maintenance of membrane structural integrity and the generation of functionally distinct membrane protein domains. Here, we show that a homolog of erythrocyte beta-spectrin (beta I sigma*) co-localizes with markers of the Golgi complex in a variety of cell ... More
Loss of intercellular junctional communication correlates with metastatic potential in mammary adenocarcinoma cells.
AuthorsNicolson GL, Dulski KM, Trosko JE
JournalProc Natl Acad Sci U S A
PubMed ID3422438
'A series of rat 13762NF mammary adenocarcinoma cell sublines and clones of various spontaneous pulmonary metastatic potentials from the mammary fat pads of syngeneic rats were examined for their intercellular junctional communication. Using the scrape-loading dye-transfer technique to introduce Lucifer yellow (Mr 457) into cells, we measured the abilities of ... More
Pattern regulation in the eyebud of Xenopus studied with a vital-dye fiber-tracing technique.
AuthorsO'Rourke NA, Fraser SE
JournalDev Biol
PubMed ID3956869
'Evidence for pattern regulation in the developing Xenopus visual system has previously been obtained after surgical manipulations of the eyebud early in development. In one experimental paradigm, a "compound" eye is produced by combining a nasal (anterior) half-eyebud with normal dorsoventral polarity and a temporal (posterior) half-eyebud with inverted dorsoventral ... More
Endocytosis of a cytotoxic human high density lipoprotein results in disruption of acidic intracellular vesicles and subsequent killing of African trypanosomes.
AuthorsHager KM, Pierce MA, Moore DR, Tytler EM, Esko JD, Hajduk SL
JournalJ Cell Biol
PubMed ID8027174
'The host range of Trypanosoma brucei brucei is restricted by the cytolytic effects of human serum high-density lipoprotein (HDL). The lytic activity is caused by a minor subclass of human serum HDL called trypanosome lytic factor (TLF). TLF binds in the flagellar pocket to specific TLF-binding sites. Internalization and localization ... More
Multiple anterograde tracing, combining Phaseolus vulgaris leucoagglutinin with rhodamine- and biotin-conjugated dextran amine.
AuthorsDolleman-Van der Weel MJ, Wouterlood FG, Witter MP
JournalJ Neurosci Methods
PubMed ID7514701
'The simultaneous use of different neuroanatomical anterograde tracers provides a potentially powerful method to study the convergence of afferent systems in a particular brain area. However, a simple routine procedure to apply multiple anterograde tracers in conjunction with their simultaneous visualization is still missing. We report an easy and straightforward ... More
Segment and cell type lineage restrictions during pharyngeal arch development in the zebrafish embryo.
AuthorsSchilling TF, Kimmel CB
JournalDevelopment
PubMed ID8162849
'In zebrafish, the segmental series of pharyngeal arches is formed predominantly by two migratory cell types, neural crest and paraxial mesoderm, which arise in the early embryo. Neural crest cells migrate ventrally out of the neuroepithelium and into the arches to form cartilage, neurons, glia and pigment cells. Surrounding mesoderm ... More
Multiple diffusion mechanisms due to nanostructuring in crowded environments.
AuthorsSanabria H, Kubota Y, Waxham MN
JournalBiophys J
PubMed ID17040979
'One of the key questions regarding intracellular diffusion is how the environment affects molecular mobility. Mostly, intracellular diffusion has been described as hindered, and the physical reasons for this behavior are: immobile barriers, molecular crowding, and binding interactions with immobile or mobile molecules. Using results from multi-photon fluorescence correlation spectroscopy, ... More
Delivery of macromolecules into live cells by simple co-incubation with a peptide.
AuthorsLee YJ, Erazo-Oliveras A, Pellois JP,
JournalChembiochem
PubMed ID20029930
'n this report, we test the hypothesis that optimized cell-penetrating peptides (CPPs) might deliver macromolecules to the cytosol of live cells by simple co-incubation and without the requirement for any type of conjugation, whether covalent or noncovalent. This hypothesis is based on the observation that the binding of TAT and ... More
Drosophila coracle, a member of the protein 4.1 superfamily, has essential structural functions in the septate junctions and developmental functions in embryonic and adult epithelial cells.
AuthorsLamb RS, Ward RE, Schweizer L, Fehon RG
JournalMol Biol Cell
PubMed ID9843584
'Although extensively studied biochemically, members of the Protein 4. 1 superfamily have not been as well characterized genetically. Studies of coracle, a Drosophila Protein 4.1 homologue, provide an opportunity to examine the genetic functions of this gene family. coracle was originally identified as a dominant suppressor of EgfrElp, a hypermorphic ... More
Calpain activity promotes the sealing of severed giant axons.
'A barrier (seal) must form at the cut ends of a severed axon if a neuron is to survive and eventually regenerate. Following severance of crayfish medial giant axons in physiological saline, vesicles accumulate at the cut end and form a barrier (seal) to ion and dye diffusion. In contrast, ... More
Efferent and collateral organization of paratrigeminal nucleus projections: an anterograde and retrograde fluorescent tracer study in the rat.
AuthorsSaxon DW, Hopkins DA
JournalJ Comp Neurol
PubMed ID9831048
'The paratrigeminal nucleus (PTN) receives primary visceral afferent projections through cranial nerves IX and X and somatic afferent projections through cranial nerve V and dorsal roots as far caudally as C7. Pressure injections of the anterograde tracer tetramethylrhodamine dextran into the PTN in the rat resulted in bilateral labeling in ... More
Study of calcium signaling in cell cleavage using confocal microscopy.
AuthorsMeng CL, Chang DC
JournalBiol Bull
PubMed ID7811793
Analysis of the growth cone turning assay for studying axon guidance.
AuthorsPujic Z, Giacomantonio CE, Unni D, Rosoff WJ, Goodhill GJ,
JournalJ Neurosci Methods
PubMed ID18313760
The
Cytoplasmic loading of dyes, protein and plasmid DNA using an impact-mediated procedure.
AuthorsClarke MS, Vanderburg CR, Hay ED, McNeil PL
JournalBiotechniques
PubMed ID7532978
We describe a method and apparatus designed to rapidly and reproducibly produce transient, survivable plasma membrane disruptions--"wounds"--in order to gain access to the cytoplasm of eukaryotic cells growing in culture. Compressed gas is used to propel glass beads, dispersed as a uniform aerosol, at adherent cells growing on a culture ... More
Membrane proximal lysosomes are the major vesicles responsible for calcium-dependent exocytosis in nonsecretory cells.
AuthorsJaiswal JK, Andrews NW, Simon SM
JournalJ Cell Biol
PubMed ID12438417
Similar to its role in secretory cells, calcium triggers exocytosis in nonsecretory cells. This calcium-dependent exocytosis is essential for repair of membrane ruptures. Using total internal reflection fluorescence microscopy, we observed that many organelles implicated in this process, including ER, post-Golgi vesicles, late endosomes, early endosomes, and lysosomes, were within ... More
Reconstitution of an operational MHC class II compartment in nonantigen-presenting cells.
AuthorsKarlsson L, Péléraux A, Lindstedt R, Liljedahl M, Peterson PA
JournalScience
PubMed ID7985028
Professional antigen-presenting cells (APCs) have a distinct compartment in which class II molecules are proposed to acquire antigenic peptides. Genetic evidence suggests that human leukocyte antigen (HLA)-DM, an unusual class II molecule, participates in this process. Peptide acquisition was reconstituted in nonprofessional APCs by transfection of class II, invariant chain ... More
AuthorsRouach N, Koulakoff A, Abudara V, Willecke K, Giaume C,
JournalScience
PubMed ID19056987
Astrocytes provide metabolic substrates to neurons in an activity-dependent manner. However, the molecular mechanisms involved in this function, as well as its role in synaptic transmission, remain unclear. Here, we show that the gap-junction subunit proteins connexin 43 and 30 allow intercellular trafficking of glucose and its metabolites through astroglial ... More
Quantitative monitoring of activity-dependent bulk endocytosis of synaptic vesicle membrane by fluorescent dextran imaging.
AuthorsClayton EL, Cousin MA,
JournalJ Neurosci Methods
PubMed ID19766140
Activity-dependent bulk endocytosis (ADBE) is the dominant synaptic vesicle (SV) retrieval mode in central nerve terminals during periods of intense neuronal activity. Despite this fact there are very few real time assays that report the activity of this critical SV retrieval mode. In this paper we report a simple and ... More
Assessing the sensitivity of commercially available fluorophores to the intracellular environment.
AuthorsChen AK, Cheng Z, Behlke MA, Tsourkas A,
JournalAnal Chem
PubMed ID18700780
The use of fluorescence has become commonplace in the biological sciences, with many studies utilizing probes based on commercially available fluorophores to provide insight into cell function and behavior. As these imaging applications become more advanced, it becomes increasingly important to acquire accurate quantitative measurements of the fluorescence signal. Absolute ... More
Selective release of molecules from Weibel-Palade bodies during a lingering kiss.
AuthorsBabich V, Meli A, Knipe L, Dempster JE, Skehel P, Hannah MJ, Carter T,
JournalBlood
PubMed ID18252862
Exocytosis of specialized endothelial cell secretory organelles, Weibel-Palade bodies (WPBs), is thought to play an important role in regulating hemostasis and intravascular inflammation. The major WPB core proteins are Von Willebrand factor (VWF) and its propolypeptide (Proregion), constituting more than 95% of the content. Although the composition of the WPBs ... More
Improved fluorescent compounds for tracing cell lineage.
AuthorsGimlich RL, Braun J
JournalDev Biol
PubMed ID2581834
In this note simple methods for the synthesis of several new fluorescent cell lineage tracers are described. These are fluorescent dextrans with average molecular weights of approximately 11 X 10(3), and with one or more fluorophore molecules covalently coupled to each dextran chain. These fluorescent dextrans are brighter than commercially ... More
Production of maternal-zygotic mutant zebrafish by germ-line replacement.
AuthorsCiruna B, Weidinger G, Knaut H, Thisse B, Thisse C, Raz E, Schier AF
JournalProc Natl Acad Sci U S A
PubMed ID12397179
We report a generally applicable strategy for transferring zygotic lethal mutations through the zebrafish germ line. By using a morpholino oligonucleotide that blocks primordial germ cell (PGC) development, we generate embryos devoid of endogenous PGCs to serve as hosts for the transplantation of germ cells derived from homozygous mutant donors. ... More
A retrograde labeling technique for the functional study of airway-specific visceral afferent neurons.
AuthorsChristian EP, Togo JA, Naper KE, Koschorke G, Taylor GA, Weinreich D
JournalJ Neurosci Methods
PubMed ID7686598
The development of a method is described whereby primary afferent neurons that specifically innervate the airways in the guinea pig can be retrogradely labeled, acutely dissociated and studied functionally with electrophysiological techniques. Following administration of either dextran-tetramethylrhodamine, Fast Blue, or Fluorogold dye into the tracheal lumen, dye-labeled neurons can be ... More
Surface biopassivation of replicated poly(dimethylsiloxane) microfluidic channels and application to heterogeneous immunoreaction with on-chip fluorescence detection.
AuthorsLinder V, Verpoorte E, Thormann W, de Rooij NF, Sigrist H
JournalAnal Chem
PubMed ID11569807
Poly(dimethylsiloxane) (PDMS) appeared recently as a material of choice for rapid and accurate replication of polymer-based microfluidic networks. However, due to its hydrophobicity, the surface strongly interacts with apolar analytes or species containing apolar domains, resulting in significant uncontrolled adsorption on channel walls. This contribution describes the application and characterization ... More
Localized calcium signals along the cleavage furrow of the Xenopus egg are not involved in cytokinesis.
AuthorsNoguchi T, Mabuchi I
JournalMol Biol Cell
PubMed ID11950937
It has been proposed that a localized calcium (Ca) signal at the growing end of the cleavage furrow triggers cleavage furrow formation in large eggs. We have examined the possible role of a Ca signal in cleavage furrow formation in the Xenopus laevis egg during the first cleavage. We were ... More
Membrane perturbation and fusion pore formation in influenza hemagglutinin-mediated membrane fusion. A new model for fusion.
AuthorsBonnafous P, Stegmann T
JournalJ Biol Chem
PubMed ID10692407
Low pH-induced fusion mediated by the hemagglutinin (HA) of influenza virus involves conformational changes in the protein that lead to the insertion of a "fusion peptide" domain of this protein into the target membrane and is thought to perturb the membrane, triggering fusion. By using whole virus, purified HA, or ... More
Acetylcholine and calcium signalling regulates muscle fibre formation in the zebrafish embryo.
AuthorsBrennan C, Mangoli M, Dyer CE, Ashworth R
JournalJ Cell Sci
PubMed ID16249237
Nerve activity is known to be an important regulator of muscle phenotype in the adult, but its contribution to muscle development during embryogenesis remains unresolved. We used the zebrafish embryo and in vivo imaging approaches to address the role of activity-generated signals, acetylcholine and intracellular calcium, in vertebrate slow muscle ... More
Electroporation loading and photoactivation of caged InsP3: tools to investigate the relation between cellular ATP release in response to intracellular InsP3 elevation.
AuthorsBraet K, Mabilde C, Cabooter L, Rapp G, Leybaert L
JournalJ Neurosci Methods
PubMed ID14687677
Photolytic liberation of InsP(3) in single cells triggers cell-to-cell propagating calcium changes that are communicated by a gap junctional and a paracrine purinergic pathway involving InsP(3)-triggered ATP release. We investigated the relation between the InsP(3) stimulus and the resulting ATP release in ECV304 cells using UV photolysis of caged compounds ... More
Functional studies of the kidney of living animals using multicolor two-photon microscopy.
AuthorsDunn KW, Sandoval RM, Kelly KJ, Dagher PC, Tanner GA, Atkinson SJ, Bacallao RL, Molitoris BA
JournalAm J Physiol Cell Physiol
PubMed ID12176747
Optical microscopy, when applied to living animals, provides a powerful means of studying cell biology in the most physiologically relevant setting. The ability of two-photon microscopy to collect optical sections deep into biological tissues has opened up the field of intravital microscopy to high-resolution studies of the brain, lens, skin, ... More
Fluorescence correlation spectroscopy close to a fluctuating membrane.
AuthorsFradin C, Abu-Arish A, Granek R, Elbaum M
JournalBiophys J
PubMed ID12609903
Compartmentalization of the cytoplasm by membranes should have a strong influence on the diffusion of macromolecules inside a cell, and we have studied how this could be reflected in fluorescence correlation spectroscopy (FCS) experiments. We derived the autocorrelation function measured by FCS for fluorescent particles diffusing close to a soft ... More
HLA-DR- and CD11c-positive dendritic cells penetrate beyond well-developed epithelial tight junctions in human nasal mucosa of allergic rhinitis.
AuthorsTakano K, Kojima T, Go M, Murata M, Ichimiya S, Himi T, Sawada N
JournalJ Histochem Cytochem
PubMed ID15872054
The epithelial barrier of the upper respiratory tract plays a crucial role in host defense. In this study, to elucidate whether there is antigen monitoring by dendritic cells (DCs) beyond the epithelial tight-junction barrier in allergic rhinitis, we investigated the expression and function of tight junctions and characterized DCs in ... More
A novel role for FGF and extracellular signal-regulated kinase in gap junction-mediated intercellular communication in the lens.
AuthorsLe AC, Musil LS
JournalJ Cell Biol
PubMed ID11449001
Gap junction-mediated intercellular coupling is higher in the equatorial region of the lens than at either pole, a property believed to be essential for lens transparency. We show that fibroblast growth factor (FGF) upregulates gap junctional intercellular dye transfer in primary cultures of embryonic chick lens cells without detectably increasing ... More
Scrape-loading and dye transfer. A rapid and simple technique to study gap junctional intercellular communication.
Authorsel-Fouly MH, Trosko JE, Chang CC
JournalExp Cell Res
PubMed ID2433137
Gap junction-mediated intercellular communication has been recognized in cells from different tissues of various organisms and has been implicated in a variety of cellular functions and dysfunctions. Here we describe a new, direct and rapid technique with which to study this cellular phenomenon. It employs scrape-loading to introduce a low ... More
Live-cell imaging methods for the study of vagal afferents within the nucleus of the solitary tract.
AuthorsRogers RC, Nasse JS, Hermann GE
JournalJ Neurosci Methods
PubMed ID16099514
Substantial evidence suggests that vagal afferent functions are modulated by agonists acting on afferent terminals in the solitary nucleus (NST). Actions of these agonists are implied through intracellular recordings from cultured nodose ganglion cells or second order NST neurons. While high-quality data have been obtained using these methods, techniques in ... More
D2-like dopamine receptors in amphibian retina: localization with fluorescent ligands.
AuthorsMuresan Z, Besharse JC
JournalJ Comp Neurol
PubMed ID8509497
Dopamine induces several light adaptive changes in amphibian retina via receptors with D2-like pharmacology, but the identity of the primary target cells has not been determined. Using a fluorescent probe consisting of a selective D2 antagonist, N-(p-aminophenethyl)-spiperone (NAPS), derivatized with the fluorophore Bodipy (NAPS-Bodipy), we identified the distribution of dopamine ... More
Dynamic behavior of rod photoreceptor disks.
AuthorsChen C, Jiang Y, Koutalos Y
JournalBiophys J
PubMed ID12202366
Eukaryotic cells use membrane organelles, like the endoplasmic reticulum or the Golgi, to carry out different functions. Vertebrate rod photoreceptors use hundreds of membrane sacs (the disks) for the detection of light. We have used fluorescent tracers and single cell imaging to study the properties of rod photoreceptor disks. Labeling ... More
Autonomous T cell trafficking examined in vivo with intravital two-photon microscopy.
AuthorsMiller MJ, Wei SH, Cahalan MD, Parker I
JournalProc Natl Acad Sci U S A
PubMed ID12601158
The recirculation of T cells between the blood and secondary lymphoid organs requires that T cells are motile and sensitive to tissue-specific signals. T cell motility has been studied in vitro, but the migratory behavior of individual T cells in vivo has remained enigmatic. Here, using intravital two-photon laser microscopy, ... More
Crayfish medial giant axons (MGAs) transected in physiological saline form vesicles which interact with each other, pre-existing vesicles, and/or with the plasmalemma to form an electrical and a physical barrier that seals a cut axonal end within 60 min. The formation of this barrier (seal) was assessed by measuring the ... More
Internalization of novel non-viral vector TAT-streptavidin into human cells.
AuthorsRinne J, Albarran B, Jylhävä J, Ihalainen TO, Kankaanpää P, Hytönen VP, Stayton PS, Kulomaa MS, Vihinen-Ranta M
JournalBMC Biotechnol
PubMed ID17199888
BACKGROUND: The cell-penetrating peptide derived from the Human immunodeficiency virus-1 transactivator protein Tat possesses the capacity to promote the effective uptake of various cargo molecules across the plasma membrane in vitro and in vivo. The objective of this study was to characterize the uptake and delivery mechanisms of a novel ... More
Spontaneous and propagated calcium release in isolated cardiac myocytes viewed by confocal microscopy.
AuthorsWilliams DA, Delbridge LM, Cody SH, Harris PJ, Morgan TO
JournalAm J Physiol
PubMed ID1550213
Laser scanning confocal microscopy of the Ca(2+)-sensitive fluorophore fluo-3 has been used to investigate spontaneous and propagated calcium release at high temporal and spatial resolution in enzymatically dispersed rat cardiomyocytes. Waves of fluorescence which propagated throughout the cytosol were evident in spontaneously contracting cardiac cells containing fluo-3, but not in ... More
Primitive, crustacean-like state of blood-brain barrier in the eye of the apterygote insect Petrobius (Archaeognatha) determined from uptake of fluorescent tracers.
AuthorsShaw SR, Varney LP
JournalJ Neurobiol
PubMed ID10590170
Compound eyes of insects in 16 orders were tested for the presence of a blood-retina barrier (BRB) by injecting the hemolymph with Procion yellow, which was excluded from the eye in all Neoptera but not in two apterygotes. A primitive apterygote (Petrobius, Machilidae) was investigated further. Epifluorescence observations with small ... More
Increased vesicle recycling in response to osmotic cell swelling. Cause and consequence of hypotonicity-provoked ATP release.
Authorsvan der Wijk T, Tomassen SF, Houtsmuller AB, de Jonge HR, Tilly BC
JournalJ Biol Chem
PubMed ID12871943
Osmotic swelling of Intestine 407 cells leads to an immediate increase in cell surface membrane area as determined using the fluorescent membrane dye FM 1-43. In addition, as measured by tetramethylrhodamine isothiocyanate (TRITC)-dextran uptake, a robust (>100-fold) increase in the rate of endocytosis was observed, starting after a discrete lag ... More
Transforming growth factor-beta 1 and forskolin modulate gap junctional communication and cellular phenotype of cultured Schwann cells.
AuthorsChandross KJ, Chanson M, Spray DC, Kessler JA
JournalJ Neurosci
PubMed ID7823132
Following peripheral nerve injury, Schwann cells undergo a series of cellular alterations that are thought to assist the regenerative process. Some of these changes are stimulated by the local release of cytokines and mitogenic factors. To test the hypothesis that cytokine regulation of gap junctional communication between cells helps to ... More
Vital dye labelling of Xenopus laevis trunk neural crest reveals multipotency and novel pathways of migration.
AuthorsCollazo A, Bronner-Fraser M, Fraser SE
JournalDevelopment
PubMed ID7693414
Although the Xenopus embryo has served as an important model system for both molecular and cellular studies of vertebrate development, comparatively little is known about its neural crest. Here, we take advantage of the ease of manipulation and relative transparency of Xenopus laevis embryos to follow neural crest cell migration ... More
The intracellular calcium increase at fertilization in Urechis caupo oocytes: activation without waves.
AuthorsStephano JL, Gould MC
JournalDev Biol
PubMed ID9356171
The intracellular Ca2+ (Cai) increase at fertilization of the marine worm Urechis caupo (Echiura) was studied with conventional and confocal epifluorescence microscopy in oocytes microinjected with calcium green dextran or dually labeled with the calcium-insensitive dye tetramethylrhodamine dextran. Calcium green fluorescence was also measured with a photomultiplier system while the ... More
The dynamin-like protein DLP1 is essential for normal distribution and morphology of the endoplasmic reticulum and mitochondria in mammalian cells.
AuthorsPitts KR, Yoon Y, Krueger EW, McNiven MA
JournalMol Biol Cell
PubMed ID10588666
The dynamin family of large GTPases has been implicated in vesicle formation from both the plasma membrane and various intracellular membrane compartments. The dynamin-like protein DLP1, recently identified in mammalian tissues, has been shown to be more closely related to the yeast dynamin proteins Vps1p and Dnm1p (42%) than to ... More
Varicosities of single sympathetic nerve terminals possess syntaxin zones and different synaptotagmin N-terminus labelling following stimulation.
AuthorsBrain KL, Cottee LJ, Bennett MR
JournalJ Neurocytol
PubMed ID9306247
A study has been made of the probability of exocytosis of synaptic vesicles at different varicosities in single sympathetic terminal axons in the mouse was deferens. An antibody (SV2Ab) against SV2. a proteoglycan in synaptic vesicles, labelled an area of individual sympathetic varicosities that was slightly less than that occupied ... More
Redistribution of cytoplasmic components during germinal vesicle breakdown in starfish oocytes.
AuthorsTerasaki M
JournalJ Cell Sci
PubMed ID7527047
The starfish oocyte is relatively clear optically, and its nucleus, termed the germinal vesicle, is large. These characteristics allowed studies by confocal microscopy of germinal vesicle breakdown during maturation in living oocytes. Three fluorescent probes for cytoplasmic components were used: fluorescein 70 kDa dextran, which does not cross the nuclear ... More
Influenza-virus-liposome lipid mixing is leaky and largely insensitive to the material properties of the target membrane.
AuthorsShangguan T, Alford D, Bentz J
JournalBiochemistry
PubMed ID8664288
Monolayer intrinsic curvature, void stabilization, and membrane rupture tension have been suggested as important factors determining the rate of membrane fusion. Here, we have studied the kinetics of fusion between influenza virus and target liposomes as a function of various target membrane material properties. In order to examine the fusion ... More
Dynamin 1 Restrains Vesicular Release to a Subquantal Mode In Mammalian Adrenal Chromaffin Cells.
Authors
JournalJ Neurosci
PubMed ID30381405
Tumor suppression in basal keratinocytes via dual non-cell-autonomous functions of a Na,K-ATPase beta subunit.
Authors
JournalElife
PubMed ID27240166
Semi-permeable Diffusion Barriers Enhance Patterning Robustness in the C. elegans Germline.
Authors
JournalDev Cell
PubMed ID26609956
Multiple Nonauditory Cortical Regions Innervate the Auditory Midbrain.
Authors
JournalJ Neurosci
PubMed ID31541020
moody encodes two GPCRs that regulate cocaine behaviors and blood-brain barrier permeability in Drosophila.
Authors
JournalCell
PubMed ID16213219
The endocytic pathway taken by cationic substances requires Rab14 but not Rab5 and Rab7.
Authors
JournalCell Rep
PubMed ID34731620
Differential Co-release of Two Neurotransmitters from a Vesicle Fusion Pore in Mammalian Adrenal Chromaffin Cells.
Authors
JournalNeuron
PubMed ID30773347
Cargo-specific recruitment in clathrin- and dynamin-independent endocytosis.
Authors
JournalNat Cell Biol
PubMed ID34616024
A Size-Selective Intracellular Delivery Platform.
Authors
JournalSmall
PubMed ID27594517
Genetic, cellular, and structural characterization of the membrane potential-dependent cell-penetrating peptide translocation pore.