'It is uncertain how antiviral lymphocytes are activated in draining lymph nodes, the site where adaptive immune responses are initiated. Here, using intravital microscopy we show that after infection of mice with vaccinia virus (a large DNA virus) or vesicular stomatitis virus (a small RNA virus), virions drained to the ... More
High density of octaarginine stimulates macropinocytosis leading to efficient intracellular trafficking for gene expression.
AuthorsKhalil IA, Kogure K, Futaki S, Harashima H
JournalJ Biol Chem
PubMed ID16326716
'The mechanism of the arginine-rich peptide-mediated cellular uptake is currently a controversial issue. Several factors, including the type of peptide, the nature of the cargo, and the linker between them, appear to affect uptake. One of the less studied factors, which may affect the uptake mechanism, is the effect of ... More
The function of intermediate filaments in cell shape and cytoskeletal integrity.
'This study describes the development and use of a specific method for disassembling intermediate filament (IF) networks in living cells. It takes advantage of the disruptive effects of mimetic peptides derived from the amino acid sequence of the helix initiation 1A domain of IF protein chains. The results demonstrate that ... More
Multiple diffusion mechanisms due to nanostructuring in crowded environments.
AuthorsSanabria H, Kubota Y, Waxham MN
JournalBiophys J
PubMed ID17040979
'One of the key questions regarding intracellular diffusion is how the environment affects molecular mobility. Mostly, intracellular diffusion has been described as hindered, and the physical reasons for this behavior are: immobile barriers, molecular crowding, and binding interactions with immobile or mobile molecules. Using results from multi-photon fluorescence correlation spectroscopy, ... More
Direct measurement of endosomal pH in living cells of the rat yolk sac epithelium by laser confocal microscopy.
AuthorsIchimura T, Hatae T, Ishida T
JournalEur J Cell Biol
PubMed ID9309389
'Endocytosis of dual-fluorescent dextran by epithelial cells was observed in the perfused rat yolk sac using a confocal laser scanning microscope. Endosomal pH was quantified from confocal images using a dual-fluorescence ratiometry technique. Changes in endosomal pH were followed over time up to 32 min with and without Brefeldin A ... More
Delivery of macromolecules into live cells by simple co-incubation with a peptide.
AuthorsLee YJ, Erazo-Oliveras A, Pellois JP,
JournalChembiochem
PubMed ID20029930
'n this report, we test the hypothesis that optimized cell-penetrating peptides (CPPs) might deliver macromolecules to the cytosol of live cells by simple co-incubation and without the requirement for any type of conjugation, whether covalent or noncovalent. This hypothesis is based on the observation that the binding of TAT and ... More
Analysis of the growth cone turning assay for studying axon guidance.
AuthorsPujic Z, Giacomantonio CE, Unni D, Rosoff WJ, Goodhill GJ,
JournalJ Neurosci Methods
PubMed ID18313760
The
Combinatorial targeting of the macropinocytotic pathway in leukemia and lymphoma cells.
AuthorsNishimura S, Takahashi S, Kamikatahira H, Kuroki Y, Jaalouk DE, O'Brien S, Koivunen E, Arap W, Pasqualini R, Nakayama H, Kuniyasu A,
JournalJ Biol Chem
PubMed ID18292083
Ligand-directed delivery of agents to leukemia and lymphoma cells has the potential to yield new mechanistic disease insights and targeted therapies. Here we set out to target the macropinocytotic pathway with a combinatorial approach. From the screening of acute T-lymphoblastic leukemia Molt-4 cells with a random phage-display peptide library, we ... More
Selective release of molecules from Weibel-Palade bodies during a lingering kiss.
AuthorsBabich V, Meli A, Knipe L, Dempster JE, Skehel P, Hannah MJ, Carter T,
JournalBlood
PubMed ID18252862
Exocytosis of specialized endothelial cell secretory organelles, Weibel-Palade bodies (WPBs), is thought to play an important role in regulating hemostasis and intravascular inflammation. The major WPB core proteins are Von Willebrand factor (VWF) and its propolypeptide (Proregion), constituting more than 95% of the content. Although the composition of the WPBs ... More
Use of fluorescent dye BCECF to measure intracellular pH in cortical collecting tubule.
AuthorsWeiner ID, Hamm LL
JournalAm J Physiol
PubMed ID2719125
The compound 2'-7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) is being extensively used to measure intracellular pH (pHi) in a variety of tissue preparations. Use of this dye in the isolated perfused rabbit cortical collecting tubule (CCT) revealed two previously unreported findings. Apical incubation via luminal perfusion with the acetoxymethyl ester of BCECF (BCECF-AM) resulted ... More
Cation-dependent fusogenicity of an N-acyl phosphatidylethanolamine.
AuthorsShangguan T, Pak CC, Ali S, Janoff AS, Meers P
JournalBiochim Biophys Acta
PubMed ID9459596
N-acyl phosphatidylethanolamines (NAPEs) are natural lipid components of many organisms. N-acylation of unsaturated phosphatidylethanolamines with a saturated fatty acid converts them from non-lamellar organizing lipids into lamellar organizing, acidic lipids which can interact with cations and potentially return to non-lamellar structures. These special properties make NAPEs candidates for fusogens. We ... More
Transcytosis maintains CFTR apical polarity in the face of constitutive and mutation-induced basolateral missorting.
Authors
JournalJ Cell Sci
PubMed ID30975917
Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination.
Authors
JournalNat Commun
PubMed ID34016994
Exportins can inhibit major mitotic assembly events in vitro: membrane fusion, nuclear pore formation, and spindle assembly.
Authors
JournalNucleus
PubMed ID32762441
Endothelial MT1-MMP targeting limits intussusceptive angiogenesis and colitis via TSP1/nitric oxide axis.
Authors
JournalEMBO Mol Med
PubMed ID31793743
Convergent extension movements and ciliary function are mediated by ofd1, a zebrafish orthologue of the human oral-facial-digital type 1 syndrome gene.
Authors
JournalHum Mol Genet
PubMed ID18971206
Automated Imaging and Analysis for the Quantification of Fluorescently Labeled Macropinosomes.
Authors
JournalJ Vis Exp
PubMed ID34515683
Culture of Intestinal Epithelial Cell Monolayers and Their Use in Multiplex Macromolecular Permeability Assays for In Vitro Analysis of Tight Junction Size Selectivity.