AuthorsHariri M, Millane G, Guimond MP, Guay G, Dennis JW, Nabi IR
JournalMol Biol Cell
PubMed ID10637306
'Transfection of Mv1Lu mink lung type II alveolar cells with beta1-6-N-acetylglucosaminyl transferase V is associated with the expression of large lysosomal vacuoles, which are immunofluorescently labeled for the lysosomal glycoprotein lysosomal-associated membrane protein-2 and the beta1-6-branched N-glycan-specific lectin phaseolis vulgaris leucoagglutinin. By electron microscopy, the vacuoles present the morphology of ... More
Gap junctional communication in the early Xenopus embryo.
AuthorsLandesman Y, Goodenough DA, Paul DL
JournalJ Cell Biol
PubMed ID10953017
'In the Xenopus embryo, blastomeres are joined by gap junctions that allow the movement of small molecules between neighboring cells. Previous studies using Lucifer yellow (LY) have reported asymmetries in the patterns of junctional communication suggesting involvement in dorso-ventral patterning. To explore that relationship, we systematically compared the transfer of ... More
Temporary disruption of the plasma membrane is required for c-fos expression in response to mechanical stress.
AuthorsGrembowicz KP, Sprague D, McNeil PL
JournalMol Biol Cell
PubMed ID10198070
'Mechanically stressed cells display increased levels of fos message and protein. Although the intracellular signaling pathways responsible for FOS induction have been extensively characterized, we still do not understand the nature of the primary cell mechanotransduction event responsible for converting an externally acting mechanical stressor into an intracellular signal cascade. ... More
Role of actin polymerization and adhesion to extracellular matrix in Rac- and Rho-induced cytoskeletal reorganization.
AuthorsMachesky LM, Hall A
JournalJ Cell Biol
PubMed ID9265656
'Most animal cells use a combination of actin-myosin-based contraction and actin polymerization- based protrusion to control their shape and motility. The small GTPase Rho triggers the formation of contractile stress fibers and focal adhesion complexes (Ridley, A.J., and A. Hall. 1992. Cell. 70:389-399) while a close relative, Rac, induces lamellipodial ... More
A role for phosphoinositide 3-kinase in the completion of macropinocytosis and phagocytosis by macrophages.
AuthorsAraki N, Johnson MT, Swanson JA
JournalJ Cell Biol
PubMed ID8947549
'Phosphoinositide 3-kinase (PI 3-kinase) has been implicated in growth factor signal transduction and vesicular membrane traffic. It is thought to mediate the earliest steps leading from ligation of cell surface receptors to increased cell surface ruffling. We show here that inhibitors of PI 3-kinase inhibit endocytosis in macrophages, not by ... More
Sorting of membrane and fluid at the apical pole of polarized Madin-Darby canine kidney cells.
AuthorsLeung SM, Ruiz WG, Apodaca G
JournalMol Biol Cell
PubMed ID10848634
'When fluid-phase markers are internalized from opposite poles of polarized Madin-Darby canine kidney cells, they accumulate in distinct apical and basolateral early endosomes before meeting in late endosomes. Recent evidence suggests that significant mixing of apically and basolaterally internalized membrane proteins occurs in specialized apical endosomal compartments, including the common ... More
Survey of the morphology of macaque retinal ganglion cells that project to the pretectum, superior colliculus, and parvicellular laminae of the lateral geniculate nucleus.
AuthorsRodieck RW, Watanabe M
JournalJ Comp Neurol
PubMed ID8308173
'In common with other vertebrates, the primate retina contains a number of different ganglion cell types that project to different regions in the brain. We wanted to determine how the different ganglion cell types, distinguished morphologically, mapped to these regions of the brain. We injected a fluorescent dye into one ... More
Micro-organization of olivocerebellar and corticonuclear connections of the paravermal cerebellum in the cat.
AuthorsGarwicz M, Apps R, Trott JR
JournalEur J Neurosci
PubMed ID8996822
'The olivocerebellar and corticonuclear connections of the forelimb area of the paravermal medial C3 zone were studied in the cat using a combined electrophysiological and fluorescent tracer technique. During an initial operation under barbiturate anaesthesia, lobules IV/V of the cerebellar anterior lobe were exposed and small injections of dextran amines ... More
Segmentation of the central nervous system in leech.
AuthorsShain DH, Stuart DK, Huang FZ, Weisblat DA
JournalDevelopment
PubMed ID10648232
'Central nervous system (CNS) in leech comprises segmentally iterated progeny derived from five embryonic lineages (M, N, O, P and Q). Segmentation of the leech CNS is characterized by the formation of a series of transverse fissures that subdivide initially continuous columns of segmental founder cells in the N lineage ... More
Cysteine-rich fibroblast growth factor receptor alters secretion and intracellular routing of fibroblast growth factor 3.
AuthorsKöhl R, Antoine M, Olwin BB, Dickson C, Kiefer P
JournalJ Biol Chem
PubMed ID10748074
'Expression of the cysteine-rich fibroblast growth factor (FGF) receptor (CFR) in COS-1 cells strongly inhibits the secretion of co-expressed FGF3. By using a column retention assay and affinity chromatography, we demonstrate that at physiological salt concentrations FGF3 binds with strong affinity to CFR in vivo and in vitro. Furthermore, to ... More
Pattern regulation in the eyebud of Xenopus studied with a vital-dye fiber-tracing technique.
AuthorsO'Rourke NA, Fraser SE
JournalDev Biol
PubMed ID3956869
'Evidence for pattern regulation in the developing Xenopus visual system has previously been obtained after surgical manipulations of the eyebud early in development. In one experimental paradigm, a "compound" eye is produced by combining a nasal (anterior) half-eyebud with normal dorsoventral polarity and a temporal (posterior) half-eyebud with inverted dorsoventral ... More
Different fates of phagocytosed particles after delivery into macrophage lysosomes.
AuthorsOh YK, Swanson JA
JournalJ Cell Biol
PubMed ID8647890
'Phagocytosis in macrophages is often studied using inert polymer microspheres. An implicit assumption in these studies is that such particles contain little or no specific information in their structure that affects their intracellular fate. We tested that assumption by examining macrophage phagosomes containing different kinds of particles and found that ... More
Quantitative microscopy of fluorescent adenovirus entry.
AuthorsNakano MY, Greber UF
JournalJ Struct Biol
PubMed ID10675297
'Fluorescence imaging of cells is a powerful tool for exploring the dynamics of organelles, proteins, and viruses. Fluorescent adenoviruses are a model system for cargo transport from the cell surface to the nucleus. Here, we describe a procedure to quantitate adenovirus-associated fluorescence in different subcellular regions. CCD camera-captured fluorescence sections ... More
Aluminum fluoride stimulates surface protrusions in cells overexpressing the ARF6 GTPase.
AuthorsRadhakrishna H, Klausner RD, Donaldson JG
JournalJ Cell Biol
PubMed ID8769418
'To study the effector function of the ADP- ribosylation factor (ARF) 6 GTP-binding protein, we transfected HeLa cells with wild-type, epitope-tagged ARF6. Previously shown to indirectly activate the ARF1 GTPase, aluminum fluoride (AIF) treatment of ARF6-transfected cells resulted in a redistribution of both ARF6 and actin to discrete sites on ... More
Fluorescent retrograde neuronal tracers that label the rat facial nucleus: a comparison of Fast Blue, Fluoro-ruby, Fluoro-emerald, Fluoro-Gold and DiI.
AuthorsChoi D, Li D, Raisman G
JournalJ Neurosci Methods
PubMed ID12100982
'Many fluorescent retrograde tracers are commercially available for neuroanatomical studies. They have been used with varying success in different models and can be very effective in the study of the facial nerve and nucleus. We compare the tracers Fast Blue (FB), Fluoro-ruby, Fluoro-emerald, Fluoro-Gold (FG), and DiI in the rat ... More
Persistent neuronal labeling by retrograde fluorescent tracers: a comparison between Fast Blue, Fluoro-Gold and various dextran conjugates.
AuthorsNovikova L, Novikov L, Kellerth JO
JournalJ Neurosci Methods
PubMed ID9210570
'The permanence of retrograde neuronal labeling by the fluorescent tracers Fast Blue, Fluoro-Gold, Mini-Ruby, Fluoro-Ruby and Fluoro-Emerald was investigated in adult rat spinal motorneurons at 1, 4, 12 and 24 weeks after tracer application to a transected muscle nerve. After 1 week, the largest number of retrogradely labeled motoneurons was ... More
Cytoplasmic loading of dyes, protein and plasmid DNA using an impact-mediated procedure.
AuthorsClarke MS, Vanderburg CR, Hay ED, McNeil PL
JournalBiotechniques
PubMed ID7532978
We describe a method and apparatus designed to rapidly and reproducibly produce transient, survivable plasma membrane disruptions--"wounds"--in order to gain access to the cytoplasm of eukaryotic cells growing in culture. Compressed gas is used to propel glass beads, dispersed as a uniform aerosol, at adherent cells growing on a culture ... More
Migration of Plasmodium sporozoites through cells before infection.
AuthorsMota MM, Pradel G, Vanderberg JP, Hafalla JC, Frevert U, Nussenzweig RS, Nussenzweig V, Rodríguez A
JournalScience
PubMed ID11141568
Intracellular bacteria and parasites typically invade host cells through the formation of an internalization vacuole around the invading pathogen. Plasmodium sporozoites, the infective stage of the malaria parasite transmitted by mosquitoes, have an alternative mechanism to enter cells. We observed breaching of the plasma membrane of the host cell followed ... More
Modern mosaic analysis in the zebrafish.
AuthorsCarmany-Rampey A, Moens CB,
JournalMethods
PubMed ID16829130
One of the most powerful tools used to gain insight into complex developmental processes is the analysis of mosaic embryos. A mosaic is defined as an organism that contains cells of more than one genotype, usually wild-type and mutant. It is the interplay between wild-type and mutant cells in the ... More
Efficacy of fluorescent tracers in retrograde labeling of cutaneous afferent neurons in the rat.
AuthorsZele T, Sketelj J, Bajrovic FF,
JournalJ Neurosci Methods
PubMed ID20600316
BACKGROUND: In the present study, the labeling efficacy of tracers Fluoro-ruby (FR), Fluoro-emerald (FE), True Blue (TB), Fluoro-Gold (FG), Diamidino Yellow (DY) and 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) to retrogradely label the cutaneous afferent neurons in the rat was examined. METHODS: The proximal stump of the transected sural nerve was exposed for ... More
A proposal for a coordinated effort for the determination of brainwide neuroanatomical connectivity in model organisms at a mesoscopic scale.
AuthorsBohland JW, Wu C, Barbas H, Bokil H, Bota M, Breiter HC, Cline HT, Doyle JC, Freed PJ, Greenspan RJ, Haber SN, Hawrylycz M, Herrera DG, Hilgetag CC, Huang ZJ, Jones A, Jones EG, Karten HJ, Kleinfeld D, Kötter R, Lester HA, Lin JM, Mensh BD, Mikula S, Panksepp J, Price JL, Safdieh J, Saper CB, Schiff ND, Schmahmann JD, Stillman BW, Svoboda K, Swanson LW, Toga AW, Van Essen DC, Watson JD, Mitra PP,
JournalPLoS Comput Biol
PubMed ID19325892
In this era of complete genomes, our knowledge of neuroanatomical circuitry remains surprisingly sparse. Such knowledge is critical, however, for both basic and clinical research into brain function. Here we advocate for a concerted effort to fill this gap, through systematic, experimental mapping of neural circuits at a mesoscopic scale ... More
Improved fluorescent compounds for tracing cell lineage.
AuthorsGimlich RL, Braun J
JournalDev Biol
PubMed ID2581834
In this note simple methods for the synthesis of several new fluorescent cell lineage tracers are described. These are fluorescent dextrans with average molecular weights of approximately 11 X 10(3), and with one or more fluorophore molecules covalently coupled to each dextran chain. These fluorescent dextrans are brighter than commercially ... More
Evx1 is a postmitotic determinant of v0 interneuron identity in the spinal cord.
Interneurons in the ventral spinal cord are essential for coordinated locomotion in vertebrates. During embryogenesis, the V0 and V1 classes of ventral interneurons are defined by expression of the homeodomain transcription factors Evx1/2 and En1, respectively. In this study, we show that Evx1 V0 interneurons are locally projecting intersegmental commissural ... More
Recent techniques for tracing pathways in the central nervous system of developing and adult mammals.
AuthorsVercelli A, Repici M, Garbossa D, Grimaldi A
JournalBrain Res Bull
PubMed ID10654576
Over the last 20 years, the choice of neural tracers has increased manyfold, and includes newly introduced anterograde tracers that allow quantitation of single-axon morphologies, and retrograde tracers that can be combined with intracellular fills for the study of dendritic arbors of neurons which have a specific projection pattern. The ... More
Rho GTPases control polarity, protrusion, and adhesion during cell movement.
AuthorsNobes CD, Hall A
JournalJ Cell Biol
PubMed ID10087266
Cell movement is essential during embryogenesis to establish tissue patterns and to drive morphogenetic pathways and in the adult for tissue repair and to direct cells to sites of infection. Animal cells move by crawling and the driving force is derived primarily from the coordinated assembly and disassembly of actin ... More
ErbB-2 amplification inhibits down-regulation and induces constitutive activation of both ErbB-2 and epidermal growth factor receptors.
AuthorsWorthylake R, Opresko LK, Wiley HS
JournalJ Biol Chem
PubMed ID10085130
ErbB-2/HER2 is an important signaling partner for the epidermal growth factor receptor (EGFR). Overexpression of erbB-2 is also associated with poor prognosis in breast cancer. To investigate how erbB-2 amplification affects its interactions with the EGFR, we used a human mammary epithelial cell system in which erbB-2 expression was increased ... More
A fate map for the 32-cell stage of Rana pipiens.
AuthorsSaint-Jeannet JP, Dawid IB
JournalDev Biol
PubMed ID7813792
A fate map of the progeny derived from all blastomeres of the 32-cell stage embryo of the leopard frog Rana pipiens has been generated. Embryos presenting regular cleavages were injected into two pairs of blastomeres with fluorescein- and Texas red-lysine dextran. By stage 21, embryos were sectioned and the tissue ... More
The functional anatomy and evolution of hypoglossal afferents in the leopard frog, Rana pipiens.
AuthorsAnderson CW, Nishikawa KC
JournalBrain Res
PubMed ID9401749
Previously, we suggested that afferents are present in the hypoglossal nerve of the leopard frog, Rana pipiens. The basis for this was behavioral data obtained after transection of the hypoglossal nerve. These afferents coordinate the timing of tongue protraction with mouth opening during feeding. The goal of the present study ... More
A sequential double labeling technique for studying changes in motoneuronal projections to muscle following nerve injury and reinnervation.
AuthorsKatada A, Vos JD, Swelstad BB, Zealear DL
JournalJ Neurosci Methods
PubMed ID16753219
The purpose of this study was to develop an anatomical technique that could directly demonstrate the motoneuron projections to the muscle both before injury and again following reinnervation. Investigation focused on the identification of a long-term retrograde fluorescent tracer that would label original motoneurons and persist long enough for reinnervating ... More
Effects of profilin and profilactin on actin structure and function in living cells.
AuthorsCao LG, Babcock GG, Rubenstein PA, Wang YL
JournalJ Cell Biol
PubMed ID1577865
Previous studies have yielded conflicting results concerning the physiological role of profilin, a 12-15-kD actin- and phosphoinositide-binding protein, as a regulator of actin polymerization. We have addressed this question by directly microinjecting mammalian profilins, prepared either from an E. coli expression system or from bovine brain, into living normal rat ... More
Clathrin-mediated endocytosis and recycling of autocrine motility factor receptor to fibronectin fibrils is a limiting factor for NIH-3T3 cell motility.
AuthorsLe PU, Benlimame N, Lagana A, Raz A, Nabi IR
JournalJ Cell Sci
PubMed ID10954421
Autocrine motility factor receptor (AMF-R) is internalized via a clathrin-independent pathway to smooth endoplasmic reticulum tubules. This endocytic pathway is shown here to be inhibited by methyl-(beta)-cyclodextrin (m(beta)CD) implicating caveolae or caveolae-like structures in AMF internalization to smooth ER. AMF-R is also internalized via a clathrin-dependent pathway to a transferrin ... More
Current concepts in neuroanatomical tracing.
AuthorsKöbbert C, Apps R, Bechmann I, Lanciego JL, Mey J, Thanos S
JournalProg Neurobiol
PubMed ID10856608
The development of new axonal tract tracing and cell labelling methods has revolutionised neurobiology in the last 30 years. The aim of this review is to consider some of the key methods of neuroanatomical tracing that are currently in use and have proved invaluable in charting the complex interconnections of ... More
Kinesin is the motor for microtubule-mediated Golgi-to-ER membrane traffic.
AuthorsLippincott-Schwartz J, Cole NB, Marotta A, Conrad PA, Bloom GS
JournalJ Cell Biol
PubMed ID7844144
The distribution and dynamics of both the ER and Golgi complex in animal cells are known to be dependent on microtubules; in many cell types the ER extends toward the plus ends of microtubules at the cell periphery and the Golgi clusters at the minus ends of microtubules near the ... More
Fluid-phase markers in the basolateral endocytic pathway accumulate in response to the actin assembly-promoting drug Jasplakinolide.
AuthorsShurety W, Stewart NL, Stow JL
JournalMol Biol Cell
PubMed ID9529391
To investigate the role of filamentous actin in the endocytic pathway, we used the cell-permeant drug Jasplakinolide (JAS) to polymerize actin in intact polarized Madin-Darby canine kidney (MDCK) cells. The uptake and accumulation of the fluid-phase markers fluorescein isothiocyanate (FITC)-dextran and horseradish peroxidase (HRP) were followed in JAS-treated or untreated ... More
Isoforms of ankyrin-3 that lack the NH2-terminal repeats associate with mouse macrophage lysosomes.
AuthorsHoock TC, Peters LL, Lux SE
JournalJ Cell Biol
PubMed ID9060470
We have recently cloned and characterized ankyrin-3 (also called ankyrin(G)), a new ankyrin that is widely distributed, especially in epithelial tissues, muscle, and neuronal axons (Peters, L.L., K.M. John, F.M. Lu, E.M. Eicher, A. Higgins, M. Yialamas, L.C. Turtzo, A.J. Otsuka, and S.E. Lux. 1995. J. Cell Biol. 130: 313-330). ... More
M-CSF-induced macropinocytosis increases solute endocytosis but not receptor-mediated endocytosis in mouse macrophages.
AuthorsRacoosin EL, Swanson JA
JournalJ Cell Sci
PubMed ID1429898
Although coated vesicles can mediate both solute and receptor-mediated endocytosis, there are other kinds of endocytic vesicles that contribute to these processes. The relative contributions of these other organelles, particularly regarding solute influx, remains unsettled. Here we describe a physiological uncoupling of solute and receptor-mediated endocytosis that occurs during growth ... More
Application of new technologies to studies of neural crest migration and differentiation.
AuthorsBronner-Fraser M, Fraser SE
JournalAm J Med Genet Suppl
PubMed ID3144984
This review describes the application of new techniques for examining some longstanding questions in the neural crest system concerning pathways of migration, cell lineage decisions, and importance of the extracellular matrix. The first issue examined involves the migratory pathways followed by neural crest cells. In birds, it has been possible ... More
Mapping of neural crest pathways in Xenopus laevis using inter- and intra-specific cell markers.
AuthorsKrotoski DM, Fraser SE, Bronner-Fraser M
JournalDev Biol
PubMed ID2452101
This study examines the pathways of migration followed by neural crest cells in Xenopus embryos using two recently described cell marking techniques. The first is an interspecific chimera created by grafting Xenopus borealis cells into Xenopus laevis hosts. The cells of these closely related species can be distinguished by their ... More
Efficacy of seven retrograde tracers, compared in multiple-labelling studies of feline motoneurones.
AuthorsRichmond FJ, Gladdy R, Creasy JL, Kitamura S, Smits E, Thomson DB
JournalJ Neurosci Methods
PubMed ID7527476
The labelling efficacies of 7 retrograde tracers were evaluated following cut nerve exposure or intramuscular injection into the serially compartmentalized neck muscle, biventer cervicis. Tested tracers included Fast Blue (FB), Fluorogold (FG), dextran conjugated to fluorescein (FD), dextran conjugated to rhodamine (Fluororuby (FR), 3000 and 10,000 MW), fluorescent latex microspheres, ... More
Signalling by TGF-beta family members: short-range effects of Xnr-2 and BMP-4 contrast with the long-range effects of activin.
AuthorsJones CM, Armes N, Smith JC
JournalCurr Biol
PubMed ID8939607
BACKGROUND: One way of establishing a morphogen gradient in a developing embryo involves the localized synthesis of an inducing molecule followed by its diffusion into surrounding tissues. The morphogen-like effects of the mesoderm-inducing factor activin provide support for this idea in amphibian development. The questions remain, however, of how activin ... More
Neurofilaments help maintain normal morphologies and support elongation of neurites in Xenopus laevis cultured embryonic spinal cord neurons.
AuthorsLin W, Szaro BG
JournalJ Neurosci
PubMed ID8613766
Neurofilament number and subunit composition, which are highly regulated during development, have been proposed to help regulate axonal diameter and stability. From experiments on dissociated cell cultures of Xenopus laevis embryonic spinal cord, we have obtained direct evidence that neurofilaments help maintain the structural integrity of newly developing axons. An ... More
Aminoglycoside antibiotics traffic to the Golgi complex in LLC-PK1 cells.
AuthorsSandoval R, Leiser J, Molitoris BA
JournalJ Am Soc Nephrol
PubMed ID9527392
Aminoglycoside antibiotics are known to be internalized via endocytosis and have been associated with subcellular organelle dysfunction; however, the route of intracellular trafficking and their distribution remain largely unknown. To address these questions, a Texas Red conjugate of gentamicin (TRG) was synthesized for dual-labeling experiments with the endoplasmic reticulum, Golgi, ... More
Macropinocytosis of protein is an amino acid supply route in Ras-transformed cells.
Authors
JournalNature
PubMed ID23665962
ORP2 couples LDL-cholesterol transport to FAK activation by endosomal cholesterol/PI(4,5)P2 exchange.
Authors
JournalEMBO J
PubMed ID34124795
Reversible silencing of lumbar spinal interneurons unmasks a task-specific network for securing hindlimb alternation.
Authors
JournalNat Commun
PubMed ID29213073
MicroRNAs are critical regulators of tuberous sclerosis complex and mTORC1 activity in the size control of the Xenopus kidney.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID24733901
Microglial NF-κB drives tau spreading and toxicity in a mouse model of tauopathy.
Authors
JournalNat Commun
PubMed ID35413950
Modulation of deregulated chaperone-mediated autophagy by a phosphopeptide.
Authors
JournalAutophagy
PubMed ID25719862
ATG7 deficiency suppresses apoptosis and cell death induced by lysosomal photodamage.
Authors
JournalAutophagy
PubMed ID22889762
Targeting Glioma Stem Cell-Derived Pericytes Disrupts the Blood-Tumor Barrier and Improves Chemotherapeutic Efficacy.
Authors
JournalCell Stem Cell
PubMed ID29100012
Multicolor two-photon imaging of in vivo cellular pathophysiology upon influenza virus infection using the two-photon IMPRESS.