Syntaxin 7 mediates endocytic trafficking to late endosomes.
AuthorsNakamura N, Yamamoto A, Wada Y, Futai M
JournalJ Biol Chem
PubMed ID10692457
'The lysosome functions are ensured by accurate membrane trafficking in the cell. We found that mouse syntaxin 7 could complement yeast vam3 and pep12 mutants defective in docking/fusion to vacuolar and prevacuolar membranes, respectively. Immunohistochemical studies showed that syntaxin 7 is localized to late endosomes, but not to early endosomes. ... More
A role for phosphoinositide 3-kinase in the completion of macropinocytosis and phagocytosis by macrophages.
AuthorsAraki N, Johnson MT, Swanson JA
JournalJ Cell Biol
PubMed ID8947549
'Phosphoinositide 3-kinase (PI 3-kinase) has been implicated in growth factor signal transduction and vesicular membrane traffic. It is thought to mediate the earliest steps leading from ligation of cell surface receptors to increased cell surface ruffling. We show here that inhibitors of PI 3-kinase inhibit endocytosis in macrophages, not by ... More
Splicing promotes rapid and efficient mRNA export in mammalian cells.
AuthorsValencia P, Dias AP, Reed R,
JournalProc Natl Acad Sci U S A
PubMed ID18287003
'The numerous steps in protein gene expression are extensively coupled to one another through complex networks of physical and functional interactions. Indeed, >25 coupled reactions, often reciprocal, have been documented among such steps as transcription, capping, splicing, and polyadenylation. Coupling is usually not essential for gene expression, but instead enhances ... More
Drosophila Squid/hnRNP helps Dynein switch from a gurken mRNA transport motor to an ultrastructural static anchor in sponge bodies.
AuthorsDelanoue R, Herpers B, Soetaert J, Davis I, Rabouille C,
JournalDev Cell
PubMed ID17925228
In Drosophila oocytes, dorso-anterior transport of gurken mRNA requires both the Dynein motor and the heterogeneous nuclear ribonucleoprotein (hnRNP) Squid. We show that gurken transcripts are transported directly on microtubules by Dynein in nonmembranous electron-dense transport particles that also contain Squid and the transport cofactors Egalitarian and Bicaudal-D. At its ... More
Functional protein delivery into neurons using polymeric nanoparticles.
AuthorsHasadsri L, Kreuter J, Hattori H, Iwasaki T, George JM,
JournalJ Biol Chem
PubMed ID19129199
An efficient route for delivering specific proteins and peptides into neurons could greatly accelerate the development of therapies for various diseases, especially those involving intracellular defects such as Parkinson disease. Here we report the novel use of polybutylcyanoacrylate nanoparticles for delivery of intact, functional proteins into neurons and neuronal cell ... More
Slow degradation of aggregates of the Alzheimer's disease amyloid beta-protein by microglial cells.
AuthorsParesce DM, Chung H, Maxfield FR
JournalJ Biol Chem
PubMed ID9361021
Microglia are immune system cells associated with senile plaques containing beta-amyloid (Abeta) in Alzheimer's disease. Although microglia are an integral part of senile plaques, their role in the development of Alzheimer's disease is not known. Because microglia are phagocytic cells, it has been suggested that microglia may function as plaque-attacking ... More
Regulation of macropinocytosis by p21-activated kinase-1.
The process of macropinocytosis is an essential aspect of normal cell function, contributing to both growth and motile processes of cells. p21-activated kinases (PAKs) are targets for activated Rac and Cdc42 guanosine 5'-triphosphatases and have been shown to regulate the actin-myosin cytoskeleton. In fibroblasts PAK1 localizes to areas of membrane ... More
Targeting of Salmonella typhimurium to vesicles containing lysosomal membrane glycoproteins bypasses compartments with mannose 6-phosphate receptors.
AuthorsGarcia-del Portillo F, Finlay BB
JournalJ Cell Biol
PubMed ID7698996
Salmonella typhimurium is an intracellular bacterial pathogen that remains enclosed in vacuoles (SCV) upon entry into the host cell. In this study we have examined the intracellular trafficking route of S. typhimurium within epithelial cells. Indirect immunofluorescence analysis showed that bacteria initiated fusion with lysosomal membrane glycoprotein (lgp)-containing compartments approximately ... More
Cellular internalization of bystander nanomaterial induced by TAT-nanoparticles and regulated by extracellular cysteine.
Authors
JournalNat Commun
PubMed ID31409778
Determining the macropinocytic index of cells through a quantitative image-based assay.
Authors
JournalNat Protoc
PubMed ID24385148
CYRI-A limits invasive migration through macropinosome formation and integrin uptake regulation.
Authors
JournalJ Cell Biol
PubMed ID34165494
Alix-mediated assembly of the actomyosin-tight junction polarity complex preserves epithelial polarity and epithelial barrier.
Authors
JournalNat Commun
PubMed ID27336173
The bone microenvironment invigorates metastatic seeds for further dissemination.
Authors
JournalCell
PubMed ID33878291
Macropinocytosis of Nab-paclitaxel Drives Macrophage Activation in Pancreatic Cancer.
Authors
JournalCancer Immunol Res
PubMed ID28108630
LRRK2 and Rab10 coordinate macropinocytosis to mediate immunological responses in phagocytes.
Authors
JournalEMBO J
PubMed ID32853409
Astrocyte plasticity in mice ensures continued endfoot coverage of cerebral blood vessels following injury and declines with age.
Authors
JournalNat Commun
PubMed ID35379828
Cancer Lipid Metabolism Confers Antiangiogenic Drug Resistance.
Authors
JournalCell Metab
PubMed ID29861385
Therapeutic paradigm of dual targeting VEGF and PDGF for effectively treating FGF-2 off-target tumors.