Viscoelastic response of fibroblasts to tension transmitted through adherens junctions.
AuthorsRagsdale GK, Phelps J, Luby-Phelps K
JournalBiophys J
PubMed ID9370474
'Cytoplasmic deformation was monitored by observing the displacements of 200-nm green fluorescent beads microinjected into the cytoplasm of Swiss 3T3 fibroblasts. We noted a novel protrusion of nonruffling cell margins that was accompanied by axial flow of beads and cytoplasmic vesicles as far as 50 microm behind the protruding plasma ... More
Five-parameter fluorescence imaging: wound healing of living Swiss 3T3 cells.
AuthorsDeBiasio R, Bright GR, Ernst LA, Waggoner AS, Taylor DL
JournalJ Cell Biol
PubMed ID2444600
'Cellular functions involve the temporal and spatial interplay of ions, metabolites, macromolecules, and organelles. To define the mechanisms responsible for completing cellular functions, we used methods that can yield both temporal and spatial information on multiple physiological parameters and chemical components in the same cell. We demonstrated that the combined ... More
Confocal microscopy of intracellular calcium dynamics during fertilization.
AuthorsStricker SA
JournalBiotechniques
PubMed ID10997262
Calcium and endoplasmic reticulum dynamics during oocyte maturation and fertilization in the marine worm Cerebratulus lacteus.
AuthorsStricker SA, Silva R, Smythe T
JournalDev Biol
PubMed ID9808782
'To monitor calcium and endoplasmic reticulum (ER) dynamics during oocyte maturation and fertilization, oocytes of the marine worm Cerebratulus lacteus were injected with the calcium-sensitive indicator calcium green dextran and/or the ER-specific probe "DiI." Based on time-lapse confocal imaging of such specimens, prophase-arrested immature oocytes failed to develop normally after ... More
Distinct roles of TIR and non-TIR regions in the subcellular localization and signaling properties of MyD88.
AuthorsNishiya T, Kajita E, Horinouchi T, Nishimoto A, Miwa S,
JournalFEBS Lett
PubMed ID17583698
MyD88 is a cytoplasmic adaptor protein that is critical for Toll-like receptor (TLR) signaling. The subcellular localization of MyD88 is characterized as large condensed forms in the cytoplasm. The mechanism and significance of this localization with respect to the signaling function, however, are currently unknown. Here, we demonstrate that MyD88 ... More
Mechanism and size cutoff for steric exclusion from actin-rich cytoplasmic domains.
AuthorsJanson LW, Ragsdale K, Luby-Phelps K
JournalBiophys J
PubMed ID8873997
Subdomains of the cytoplasmic volume in tissue culture cells exclude large tracer particles relative to small. Evidence suggests that exclusion of the large particles is due to molecular sieving by the dense meshwork of microfilaments found in these compartments, but exclusion as a result of the close apposition of the ... More
Calcium waves along the cleavage furrows in cleavage-stage Xenopus embryos and its inhibition by heparin.
AuthorsMuto A, Kume S, Inoue T, Okano H, Mikoshiba K
JournalJ Cell Biol
PubMed ID8858172
Calcium signaling is known to be associated with cytokinesis; however, the detailed spatio-temporal pattern of calcium dynamics has remained unclear. We have studied changes of intracellular free calcium in cleavage-stage Xenopus embryos using fluorescent calcium indicator dyes, mainly Calcium Green-1. Cleavage formation was followed by calcium transients that localized to ... More
Differential targeting of protein kinase C and CaM kinase II signalings to vimentin.
AuthorsOgawara M, Inagaki N, Tsujimura K, Takai Y, Sekimata M, Ha MH, Imajoh-Ohmi S, Hirai S, Ohno S, Sugiura H
JournalJ Cell Biol
PubMed ID7490282
Hydrolysis of inositol phospholipids by receptor stimulation activates two separate signaling pathways, one leading to the activation of protein kinase C (C kinase) via formation of diacylglycerol. The other is the inositol trisphosphate (IP3)/Ca2+ pathway and a major downstream kinase which is activated is Ca2+/calmodulin-dependent protein kinase II (CaM kinase ... More
Two-photon in vivo microscopy of sulfonefluorescein secretion in normal and cystic rat kidneys.
AuthorsTanner GA, Sandoval RM, Dunn KW
JournalAm J Physiol Renal Physiol
PubMed ID12965895
Sulfonefluorescein (SF) is a fluorescent organic anion secreted by kidney proximal tubules. The purposes of this study were 1) to quantify accumulation of SF in normal and cystic rat kidneys in vivo and 2) to test whether SF accumulation could be used as a marker for cysts derived from proximal ... More
Effects of Bafilomycin A1: an inhibitor of vacuolar H (+)-ATPases on endocytosis and apoptosis in RAW cells and RAW cell-derived osteoclasts.
AuthorsXu J, Feng HT, Wang C, Yip KH, Pavlos N, Papadimitriou JM, Wood D, Zheng MH
JournalJ Cell Biochem
PubMed ID12647307
Bafilomycin A1, a specific inhibitor of V-ATPases, is a potent inhibitor of bone resorption, but the underlying mechanisms of its action remain unclear. In this study, we investigated the effect of Bafilomycin A1 on endocytosis and apoptosis in RAW cells and RAW cell-derived osteoclasts. Quantitative analysis by flow cytometry showed ... More
Cytoplasmic calcium gradients and calmodulin in the early development of the fucoid alga Pelvetia compressa.
AuthorsPu R, Robinson KR
JournalJ Cell Sci
PubMed ID9763514
The predicted existence of cytoplasmic Ca2+ gradients during the photopolarization of the zygotes of the brown algae, Pelvetia and Fucus, has proved to be difficult to establish, and the downstream targets of the putative gradients are not known. We have used quantitative microinjection of the long excitation wavelength Ca2+ indicator, ... More
Evidence against defective trans-Golgi acidification in cystic fibrosis.
AuthorsSeksek O, Biwersi J, Verkman AS
JournalJ Biol Chem
PubMed ID8663158
Defective organelle acidification has been proposed as a unifying hypothesis to explain the pleiotropic cellular abnormalities associated with cystic fibrosis. To test whether cystic fibrosis transmembrane conductance regulator (CFTR) participates in trans-Golgi pH regulation, intraluminal trans-Golgi pH was measured in stably transfected Swiss 3T3 fibroblasts (expressing CFTR or DeltaF508-CFTR) and ... More
Time-lapse confocal imaging of calcium dynamics in starfish embryos.
AuthorsStricker SA
JournalDev Biol
PubMed ID7649379
During fertilization and cleavage, embryos undergo transient rises in their intracellular free calcium levels that are postulated to provide essential signals enabling normal development to proceed. In order to analyze the spatiotemporal patterns and possible biological significance of these calcium transients, time-lapse confocal microscopy was used to monitor starfish embryos ... More
PDGF controls contact inhibition of locomotion by regulating N-cadherin during neural crest migration.
Authors
JournalDevelopment
PubMed ID28526750
The mechanosensitive channel Piezo1 cooperates with semaphorins to control neural crest migration.
Authors
JournalDevelopment
PubMed ID34822717
PG545, an angiogenesis and heparanase inhibitor, reduces primary tumor growth and metastasis in experimental pancreatic cancer.
Authors
JournalMol Cancer Ther
PubMed ID23696215
Par3 controls neural crest migration by promoting microtubule catastrophe during contact inhibition of locomotion.
Authors
JournalDevelopment
PubMed ID24173803
Galectin-1-driven T cell exclusion in the tumor endothelium promotes immunotherapy resistance.
Authors
JournalJ Clin Invest
PubMed ID31710313
Endodermal germ-layer formation through active actin-driven migration triggered by N-cadherin.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID28874564
Crumbs organizes the transport machinery by regulating apical levels of PI(4,5)P2 in Drosophila.
Authors
JournalElife
PubMed ID31697234
A Potent Anti-Malarial Human Monoclonal Antibody Targets Circumsporozoite Protein Minor Repeats and Neutralizes Sporozoites in the Liver.
Authors
JournalImmunity
PubMed ID32946741
The Rab11-binding protein RELCH/KIAA1468 controls intracellular cholesterol distribution.
Authors
JournalJ Cell Biol
PubMed ID29514919
Mechanisms of Silver Nanoparticle Uptake by Embryonic Zebrafish Cells.