Cytoplasmic transport in Drosophila ovarian follicles: the migration of microinjected fluorescent probes through intercellular bridges depends neither on electrical charge nor on external osmolarity.
AuthorsBohrmann J, Schill S
JournalInt J Dev Biol
PubMed ID9240567
'Using video-intensified fluorescence microscopy and a pseudocolor display of fluorescence intensity, we analyzed the distribution of microinjected molecules within the nurse-cell/oocyte syncytium of Drosophila ovarian follicles. We varied the composition and the osmolarity of the culture solution as well as the electrical charge and the molecular mass of the microinjected ... More
Neuropeptides exert direct effects on rat thymic epithelial cells in culture.
AuthorsHead GM, Mentlein R, von Patay B, Downing JE, Kendall MD
JournalDev Immunol
PubMed ID9716910
'To determine if major thymic neuropeptides and neurotransmitters can directly influence the functional activity of cultured rat thymic epithelium, neuropeptides and neurotransmitters were applied, and intercellular communication, proliferation, and thymulin secretion assessed. After injections of a mixture of lucifer yellow dextran (too large to pass gap junctions) and cascade blue ... More
Direct functional assay for tobacco mosaic virus cell-to-cell movement protein and identification of a domain involved in increasing plasmodesmal permeability.
AuthorsWaigmann E, Lucas WJ, Citovsky V, Zambryski P
JournalProc Natl Acad Sci U S A
PubMed ID8108427
'Plasmodesmata are cytoplasmic bridges between plant cells thought to generally allow only the passage of small molecules and metabolites. However, large structures such as plant viruses also move from cell to cell via plasmodesmata. In tobacco mosaic virus (TMV) infection a viral movement protein (TMV-MP) mediates viral spread. Here, a ... More
A galinstan expansion femtosyringe for microinjection of eukaryotic organelles and prokaryotes.
AuthorsKnoblauch M, Hibberd JM, Gray JC, van Bel AJ
JournalNat Biotechnol
PubMed ID10471935
'A galinstan expansion femtosyringe enables femtoliter to attoliter samples to be introduced into prokaryotes and subcellular compartments of eukaryotes. The method uses heat-induced expansion of galinstan (a liquid metal alloy of gallium, indium, and tin) within a glass syringe to expel samples through a tip diameter of about 0.1 microm. ... More
Restricted movement of lipid and aqueous dyes through pores formed by influenza hemagglutinin during cell fusion.
AuthorsZimmerberg J, Blumenthal R, Sarkar DP, Curran M, Morris SJ
JournalJ Cell Biol
PubMed ID7806567
The fusion of cells by influenza hemagglutinin (HA) is the best characterized example of protein-mediated membrane fusion. In simultaneous measurements of pairs of assays for fusion, we determined the order of detectable events during fusion. Fusion pore formation in HA-triggered cell-cell fusion was first detected by changes in cell membrane ... More
Neural organization and visual processing in the anterior optic tubercle of the honeybee brain.
AuthorsMota T, Yamagata N, Giurfa M, Gronenberg W, Sandoz JC,
JournalJ Neurosci
PubMed ID21832175
The honeybee Apis mellifera represents a valuable model for studying the neural segregation and integration of visual information. Vision in honeybees has been extensively studied at the behavioral level and, to a lesser degree, at the physiological level using intracellular electrophysiological recordings of single neurons. However, our knowledge of visual ... More
Correlative microscopy of densely labeled projection neurons using neural tracers.
AuthorsOberti D, Kirschmann MA, Hahnloser RH,
JournalFront Neuroanat
PubMed ID20676237
Three-dimensional morphological information about neural microcircuits is of high interest in neuroscience, but acquiring this information remains challenging. A promising new correlative technique for brain imaging is array tomography (Micheva and Smith, 2007), in which series of ultrathin brain sections are treated with fluorescent antibodies against neurotransmitters and synaptic proteins. ... More
Modulation of dye-coupling and proliferation in cultured rat thymic epithelium by factors involved in thymulin secretion.
AuthorsHead GM, Mentlein R, Kranz A, Downing JE, Kendall MD
JournalJ Anat
PubMed ID9418992
Cultures of rat thymic epithelium were used to measure the effect of thymulin secretagogues on dye-coupling and proliferation. Dye-coupling was assessed after the injection of lucifer yellow dextran which cannot permeate the connexin pore of gap junctions and the smaller, permeant cascade blue. In addition to gap junctional communication, larger ... More
Characterization of mitotic motors by their relative sensitivity to AMP-PNP.
AuthorsLee GM
JournalJ Cell Sci
PubMed ID2698890
The relative sensitivities of the motors for mitotic chromosome movements and saltatory motion were compared using a nonhydrolyzable analog of ATP, AMP-PNP. K+AMP-PNP was microinjected into PtKl cells at the time of nuclear envelope disassembly or at anaphase onset. To produce a dose-response curve for the effect of AMP-PNP on ... More