Intravital two-photon microscopy for studying the uptake and trafficking of fluorescently conjugated molecules in live rodents.
AuthorsMasedunskas A, Weigert R,
JournalTraffic
PubMed ID18647170
'In this study, we describe an experimental system based on intravital two-photon microscopy for studying endocytosis in live animals. The rodent submandibular glands were chosen as model organs because they can be exposed easily, imaged without compromising their function and, furthermore, they are amenable to pharmacological and genetic manipulations. We ... More
Formation of neurofibrillary tangles in P301l tau transgenic mice induced by Abeta 42 fibrils.
AuthorsGötz J, Chen F, van Dorpe J, Nitsch RM
JournalScience
PubMed ID11520988
'beta-Amyloid plaques and neurofibrillary tangles (NFTs) are the defining neuropathological hallmarks of Alzheimer''s disease, but their pathophysiological relation is unclear. Injection of beta-amyloid Abeta42 fibrils into the brains of P301L mutant tau transgenic mice caused fivefold increases in the numbers of NFTs in cell bodies within the amygdala from where ... More
A functional YNKI motif in the short cytoplasmic tail of varicella-zoster virus glycoprotein gH mediates clathrin-dependent and antibody-independent endocytosis.
AuthorsPasieka TJ, Maresova L, Grose C,
JournalJ Virol
PubMed ID12634377
'The trafficking of varicella-zoster virus (VZV) gH was investigated under both infection and transfection conditions. In initial endocytosis assays performed in infected cells, the three glycoproteins gE, gI, and gB served as positive controls for internalization from the plasma membrane. Subsequently, we discovered that gH in VZV-infected cells was also ... More
Heterogeneous proliferative potential in regenerative adult newt cardiomyocytes.
AuthorsBettencourt-Dias M, Mittnacht S, Brockes JP
JournalJ Cell Sci
PubMed ID12928330
'Adult newt cardiomyocytes, in contrast to their mammalian counterparts, can proliferate after injury and contribute to the functional regeneration of the heart. In order to understand the mechanisms underlying this plasticity we performed longitudinal studies on single cardiomyocytes in culture. We find that the majority of cardiomyocytes can enter S ... More
A role for phosphoinositide 3-kinase in the completion of macropinocytosis and phagocytosis by macrophages.
AuthorsAraki N, Johnson MT, Swanson JA
JournalJ Cell Biol
PubMed ID8947549
'Phosphoinositide 3-kinase (PI 3-kinase) has been implicated in growth factor signal transduction and vesicular membrane traffic. It is thought to mediate the earliest steps leading from ligation of cell surface receptors to increased cell surface ruffling. We show here that inhibitors of PI 3-kinase inhibit endocytosis in macrophages, not by ... More
Pattern regulation in the eyebud of Xenopus studied with a vital-dye fiber-tracing technique.
AuthorsO'Rourke NA, Fraser SE
JournalDev Biol
PubMed ID3956869
'Evidence for pattern regulation in the developing Xenopus visual system has previously been obtained after surgical manipulations of the eyebud early in development. In one experimental paradigm, a "compound" eye is produced by combining a nasal (anterior) half-eyebud with normal dorsoventral polarity and a temporal (posterior) half-eyebud with inverted dorsoventral ... More
Functional increases in cerebral blood volume over somatosensory cortex.
AuthorsNarayan SM, Esfahani P, Blood AJ, Sikkens L, Toga AW
JournalJ Cereb Blood Flow Metab
PubMed ID7673370
'We have examined the relationship between cerebral blood volume (CBV) and electrophysiology over primary somatosensory cortex (S-I) in the rat. We did this by comparing the spatial characteristics and time course of activity-related changes in plasma fluorescence, intrinsic optical reflectance signals, and single unit electrophysiology in S-I to identical stimuli. ... More
In vivo diffusion analysis with quantum dots and dextrans predicts the width of brain extracellular space.
AuthorsThorne RG, Nicholson C
JournalProc Natl Acad Sci U S A
PubMed ID16567637
'Diffusion within the extracellular space (ECS) of the brain is necessary for chemical signaling and for neurons and glia to access nutrients and therapeutics; however, the width of the ECS in living tissue remains unknown. We used integrative optical imaging to show that dextrans and water-soluble quantum dots with Stokes-Einstein ... More
Delivery of macromolecules into live cells by simple co-incubation with a peptide.
AuthorsLee YJ, Erazo-Oliveras A, Pellois JP,
JournalChembiochem
PubMed ID20029930
'n this report, we test the hypothesis that optimized cell-penetrating peptides (CPPs) might deliver macromolecules to the cytosol of live cells by simple co-incubation and without the requirement for any type of conjugation, whether covalent or noncovalent. This hypothesis is based on the observation that the binding of TAT and ... More
Requirement for annexin A1 in plasma membrane repair.
AuthorsMcNeil AK, Rescher U, Gerke V, McNeil PL
JournalJ Biol Chem
PubMed ID16984915
'Ca2+ entering a cell through a torn or disrupted plasma membrane rapidly triggers a combination of homotypic and exocytotic membrane fusion events. These events serve to erect a reparative membrane patch and then anneal it to the defect site. Annexin A1 is a cytosolic protein that, when activated by micromolar ... More
Visualization and identification of IL-7 producing cells in reporter mice.
AuthorsMazzucchelli RI, Warming S, Lawrence SM, Ishii M, Abshari M, Washington AV, Feigenbaum L, Warner AC, Sims DJ, Li WQ, Hixon JA, Gray DH, Rich BE, Morrow M, Anver MR, Cherry J, Naf D, Sternberg LR, McVicar DW, Farr AG, Germain RN, Rogers K, Jenkins NA, Copeland NG, Durum SK,
JournalPLoS One
PubMed ID19907640
Interleukin-7 (IL-7) is required for lymphocyte development and homeostasis although the actual sites of IL-7 production have never been clearly identified. We produced a bacterial artificial chromosome (BAC) transgenic mouse expressing ECFP in the Il7 locus. The construct lacked a signal peptide and ECFP (enhanced cyan fluorescent protein) accumulated inside ... More
Activated phosphatidylinositol 3-kinase and Akt kinase promote survival of superior cervical neurons.
AuthorsPhilpott KL, McCarthy MJ, Klippel A, Rubin LL
JournalJ Cell Biol
PubMed ID9348296
The signaling pathways that mediate the ability of NGF to support survival of dependent neurons are not yet completely clear. However previous work has shown that the c-Jun pathway is activated after NGF withdrawal, and blocking this pathway blocks neuronal cell death. In this paper we show that over-expression in ... More
Noninvasive in vivo imaging of pancreatic islet cell biology.
AuthorsSpeier S, Nyqvist D, Cabrera O, Yu J, Molano RD, Pileggi A, Moede T, Köhler M, Wilbertz J, Leibiger B, Ricordi C, Leibiger IB, Caicedo A, Berggren PO,
JournalNat Med
PubMed ID18327249
Advanced imaging techniques have become a valuable tool in the study of complex biological processes at the cellular level in biomedical research. Here, we introduce a new technical platform for noninvasive in vivo fluorescence imaging of pancreatic islets using the anterior chamber of the eye as a natural body window. ... More
A c-Jun dominant negative mutant protects sympathetic neurons against programmed cell death.
AuthorsHam J, Babij C, Whitfield J, Pfarr CM, Lallemand D, Yaniv M, Rubin LL
JournalNeuron
PubMed ID7748560
Sympathetic neurons depend on nerve growth factor (NGF) for survival and die by apoptosis in its absence. We have investigated the pattern of expression of the Jun and Fos family of transcription factors in dying sympathetic neurons using antibodies specific for each family member. When sympathetic neurons are deprived of ... More
A novel nuclear export signal and a REF interaction domain both promote mRNA export by the Epstein-Barr virus EB2 protein.
AuthorsHiriart E, Farjot G, Gruffat H, Nguyen MV, Sergeant A, Manet E
JournalJ Biol Chem
PubMed ID12403791
A striking characteristic of mRNA export factors is that they shuttle continuously between the cytoplasm and the nucleus. This shuttling is mediated by specific factors interacting with peptide motifs called nuclear export signals (NES) and nuclear localization signals. We have identified a novel CRM-1-independent transferable NES and two nuclear localization ... More
In vivo imaging of reactive oxygen species specifically associated with thioflavine S-positive amyloid plaques by multiphoton microscopy.
Amyloid-beta, the primary constituent of senile plaques in Alzheimer's disease, is hypothesized to cause neuronal damage and cognitive failure, but the mechanisms are unknown. Using multiphoton imaging, we show a direct association between amyloid-beta deposits and free radical production in vivo in live, transgenic mouse models of Alzheimer's disease and ... More
Distinct functions of nuclear and cytoplasmic calcium in the control of gene expression.
AuthorsHardingham GE, Chawla S, Johnson CM, Bading H
JournalNature
PubMed ID9000075
Calcium entry into neuronal cells through voltage or ligand-gated ion channels triggers neuronal activity-dependent gene expression critical for adaptive changes in the nervous system. Cytoplasmic calcium transients are often accompanied by an increase in the concentration of nuclear calcium, but the functional significance of such spatially distinct calcium signals is ... More
Reconstitution of nuclear protein export in isolated nuclear envelopes.
AuthorsSiebrasse JP, Coutavas E, Peters R
JournalJ Cell Biol
PubMed ID12196506
Signal-dependent nuclear protein export was studied in perforated nuclei and isolated nuclear envelopes of Xenopus oocytes by optical single transporter recording. Manually isolated and purified oocyte nuclei were attached to isoporous filters and made permeable for macromolecules by perforation. Export of a recombinant protein (GG-NES) containing the nuclear export signal ... More
Visualization and tracking of single protein molecules in the cell nucleus.
AuthorsKues T, Peters R, Kubitscheck U
JournalBiophys J
PubMed ID11371468
A recently developed laser fluorescence videomicroscopy method was used to determine for the first time the intranuclear trajectories of single protein molecules. Using the recombinant Escherichia coli beta-galactosidase protein P4K, labeled with an average of 4.6 ALEXA 488 chromophores per tetramer, single P4K molecules could be localized and tracked in ... More
Hindered diffusion of high molecular weight compounds in brain extracellular microenvironment measured with integrative optical imaging.
AuthorsNicholson C, Tao L
JournalBiophys J
PubMed ID7508761
This paper describes the theory of an integrative optical imaging system and its application to the analysis of the diffusion of 3-, 10-, 40-, and 70-kDa fluorescent dextran molecules in agarose gel and brain extracellular microenvironment. The method uses a precisely defined source of fluorescent molecules pressure ejected from a ... More
Targeted two-photon chemical apoptotic ablation of defined cell types in vivo.
Authors
JournalNat Commun
PubMed ID28621306
Simultaneous downregulation of KLF5 and Fli1 is a key feature underlying systemic sclerosis.
Authors
JournalNat Commun
PubMed ID25504335
Microfabricated blood vessels for modeling the vascular transport barrier.
Authors
JournalNat Protoc
PubMed ID30953042
Brain capillary pericytes exert a substantial but slow influence on blood flow.
Authors
JournalNat Neurosci
PubMed ID33603231
Apelin+ Endothelial Niche Cells Control Hematopoiesis and Mediate Vascular Regeneration after Myeloablative Injury.
Authors
JournalCell Stem Cell
PubMed ID31761723
Programming of Distinct Chemokine-Dependent and -Independent Search Strategies for Th1 and Th2 Cells Optimizes Function at Inflamed Sites.
Authors
JournalImmunity
PubMed ID31399281
Aged blood impairs hippocampal neural precursor activity and activates microglia via brain endothelial cell VCAM1.
Authors
JournalNat Med
PubMed ID31086348
Neuropathic pain caused by miswiring and abnormal end organ targeting.
Authors
JournalNature
PubMed ID35614217
Local Mitochondrial ATP Production Regulates Endothelial Fatty Acid Uptake and Transport.
Authors
JournalCell Metab
PubMed ID32521232
A branched-chain amino acid metabolite drives vascular fatty acid transport and causes insulin resistance.