Permeabilization and recovery of the stratum corneum in vivo: the synergy of photomechanical waves and sodium lauryl sulfate.
AuthorsLee S, McAuliffe DJ, Kollias N, Flotte TJ, Doukas AG
JournalLasers Surg Med
PubMed ID11553902
'BACKGROUND AND OBJECTIVE: Photomechanical waves render the stratum corneum permeable and allow macromolecules to diffuse into the epidermis and dermis. The aim of this study was to investigate the combined action of photomechanical waves and sodium lauryl sulfate, an anionic surfactant, for transdermal delivery. STUDY DESIGN/MATERIALS AND METHODS: A single ... More
Photomechanical transdermal delivery: the effect of laser confinement.
AuthorsLee S, McAuliffe DJ, Flotte TJ, Kollias N, Doukas AG
JournalLasers Surg Med
PubMed ID11344515
BACKGROUND AND OBJECTIVE: Photomechanical waves can transiently permeabilize the stratum corneum and facilitate the delivery of drugs into the epidermis and dermis. The present study was undertaken to assess the effect of pulse characteristics to the penetration depth of macromolecules delivered into the skin. STUDY DESIGN/MATERIALS AND METHODS: Photomechanical waves ... More
Imaging of endosome fusion in BHK fibroblasts based on a novel fluorimetric avidin-biotin binding assay.
AuthorsEmans N, Biwersi J, Verkman AS
JournalBiophys J
PubMed ID8527685
A fluorescence assay of in vivo endosome fusion was developed and applied to define the kinetics of endosome fusion in baby hamster kidney (BHK) fibroblasts. The assay is based on an approximately 10-fold enhancement of the green fluorescence of BODIPY-avidin upon biotin binding. The BODIPY-avidin fluorescence enhancement occurred in < ... More