Multiple diffusion mechanisms due to nanostructuring in crowded environments.
AuthorsSanabria H, Kubota Y, Waxham MN
JournalBiophys J
PubMed ID17040979
'One of the key questions regarding intracellular diffusion is how the environment affects molecular mobility. Mostly, intracellular diffusion has been described as hindered, and the physical reasons for this behavior are: immobile barriers, molecular crowding, and binding interactions with immobile or mobile molecules. Using results from multi-photon fluorescence correlation spectroscopy, ... More
Analysis of the growth cone turning assay for studying axon guidance.
AuthorsPujic Z, Giacomantonio CE, Unni D, Rosoff WJ, Goodhill GJ,
JournalJ Neurosci Methods
PubMed ID18313760
The
Quantitative monitoring of activity-dependent bulk endocytosis of synaptic vesicle membrane by fluorescent dextran imaging.
AuthorsClayton EL, Cousin MA,
JournalJ Neurosci Methods
PubMed ID19766140
Activity-dependent bulk endocytosis (ADBE) is the dominant synaptic vesicle (SV) retrieval mode in central nerve terminals during periods of intense neuronal activity. Despite this fact there are very few real time assays that report the activity of this critical SV retrieval mode. In this paper we report a simple and ... More
Selective release of molecules from Weibel-Palade bodies during a lingering kiss.
AuthorsBabich V, Meli A, Knipe L, Dempster JE, Skehel P, Hannah MJ, Carter T,
JournalBlood
PubMed ID18252862
Exocytosis of specialized endothelial cell secretory organelles, Weibel-Palade bodies (WPBs), is thought to play an important role in regulating hemostasis and intravascular inflammation. The major WPB core proteins are Von Willebrand factor (VWF) and its propolypeptide (Proregion), constituting more than 95% of the content. Although the composition of the WPBs ... More
Amino acid sequence requirements of the transmembrane and cytoplasmic domains of influenza virus hemagglutinin for viable membrane fusion.
AuthorsMelikyan GB, Lin S, Roth MG, Cohen FS
JournalMol Biol Cell
PubMed ID10359599
The amino acid sequence requirements of the transmembrane (TM) domain and cytoplasmic tail (CT) of the hemagglutinin (HA) of influenza virus in membrane fusion have been investigated. Fusion properties of wild-type HA were compared with those of chimeras consisting of the ectodomain of HA and the TM domain and/or CT ... More
The lipid-anchored ectodomain of influenza virus hemagglutinin (GPI-HA) is capable of inducing nonenlarging fusion pores.
AuthorsMarkosyan RM, Cohen FS, Melikyan GB
JournalMol Biol Cell
PubMed ID10749920
GPI-linked hemagglutinin (GPI-HA) of influenza virus was thought to induce hemifusion without pore formation. Cells expressing either HA or GPI-HA were bound to red blood cells, and their fusion was compared by patch-clamp capacitance measurements and fluorescence microscopy. It is now shown that under more optimal fusion conditions than have ... More
Inner but not outer membrane leaflets control the transition from glycosylphosphatidylinositol-anchored influenza hemagglutinin-induced hemifusion to full fusion.
AuthorsMelikyan GB, Brener SA, Ok DC, Cohen FS
JournalJ Cell Biol
PubMed ID9060465
Cells that express wild-type influenza hemagglutinin (HA) fully fuse to RBCs, while cells that express the HA-ectodomain anchored to membranes by glycosylphosphatidylinositol, rather than by a transmembrane domain, only hemifuse to RBCs. Amphipaths were inserted into inner and outer membrane leaflets to determine the contribution of each leaflet in the ... More
NFAT signaling in human mesenchymal stromal cells affects extracellular matrix remodeling and antifungal immune responses.
Authors
JournaliScience
PubMed ID34195564
Dynamin 1 Restrains Vesicular Release to a Subquantal Mode In Mammalian Adrenal Chromaffin Cells.
Authors
JournalJ Neurosci
PubMed ID30381405
A versatile microfluidic device for multiple ex vivo/in vitro tissue assays unrestrained from tissue topography.
Authors
JournalMicrosyst Nanoeng
PubMed ID34567653
Lymph Flow Induces the Postnatal Formation of Mature and Functional Meningeal Lymphatic Vessels.
Authors
JournalFront Immunol
PubMed ID31993056
Cerebral amyloid angiopathy aggravates perivascular clearance impairment in an Alzheimer's disease mouse model.
Authors
JournalActa Neuropathol Commun
PubMed ID33153499
Lyophilized, thermostable Spike or RBD immunogenic liposomes induce protective immunity against SARS-CoV-2 in mice.
Authors
JournalSci Adv
PubMed ID34851667
Modulation of occluding junctions alters the hematopoietic niche to trigger immune activation.
Authors
JournalElife
PubMed ID28841136
Differential Co-release of Two Neurotransmitters from a Vesicle Fusion Pore in Mammalian Adrenal Chromaffin Cells.
Authors
JournalNeuron
PubMed ID30773347
Analysis of neuronal Ca2+ handling properties by combining perforated patch clamp recordings and the added buffer approach.
Authors
JournalCell Calcium
PubMed ID34082340
Vascular and Neurogenic Rejuvenation in Aging Mice by Modulation of ASM.