Monitoring the permeability of the nuclear envelope during the cell cycle.
AuthorsLénárt P, Ellenberg J,
JournalMethods
PubMed ID16343937
'In animal organisms the nuclear envelope (NE) dis-assembles during cell division resulting in complete intermixing of cytoplasmic and nuclear compartments. This leads to the activation of many mitotic enzymes, which were kept away from their substrates or regulators by nuclear or cytoplasmic sequestration in interphase. Nuclear envelope breakdown (NEBD) is ... More
Fluorescence correlation microscopy of cells in the presence of autofluorescence.
AuthorsBrock R, Hink MA, Jovin TM
JournalBiophys J
PubMed ID9788950
'Fluorescence correlation microscopy (FCM), the combination of fluorescence correlation spectroscopy (FCS) and digital microscopy (Brock and Jovin, 1998. Cell. Mol. Biol. 44:847-856), has been implemented for measuring molecular diffusion and association in living cells with explicit consideration of autocorrelations arising from autofluorescence. Autofluorescence excited at 532 nm colocalizes with mitochondria, ... More
Localized calcium signals along the cleavage furrow of the Xenopus egg are not involved in cytokinesis.
AuthorsNoguchi T, Mabuchi I
JournalMol Biol Cell
PubMed ID11950937
It has been proposed that a localized calcium (Ca) signal at the growing end of the cleavage furrow triggers cleavage furrow formation in large eggs. We have examined the possible role of a Ca signal in cleavage furrow formation in the Xenopus laevis egg during the first cleavage. We were ... More
Mycobacterium avium- and Mycobacterium tuberculosis-containing vacuoles are dynamic, fusion-competent vesicles that are accessible to glycosphingolipids from the host cell plasmalemma.
AuthorsRussell DG, Dant J, Sturgill-Koszycki S
JournalJ Immunol
PubMed ID8648123
The vacuoles inhabited by viable Mycobacterium avium and Mycobacterium tuberculosis show limited fusion with endosomal and lysosomal compartments. This ability to regulate the maturation of their phagosomal compartments and restrict their differentiation into hydrolytically active vacuoles appears to correlate with the survival of the bacilli. Data presented in this current ... More
Imaging of endosome fusion in BHK fibroblasts based on a novel fluorimetric avidin-biotin binding assay.
AuthorsEmans N, Biwersi J, Verkman AS
JournalBiophys J
PubMed ID8527685
A fluorescence assay of in vivo endosome fusion was developed and applied to define the kinetics of endosome fusion in baby hamster kidney (BHK) fibroblasts. The assay is based on an approximately 10-fold enhancement of the green fluorescence of BODIPY-avidin upon biotin binding. The BODIPY-avidin fluorescence enhancement occurred in < ... More
Spatiotemporal control of embryonic gene expression using caged morpholinos.
Authors
JournalMethods Cell Biol
PubMed ID21924162
Myeloid Cell-Targeted Nanocarriers Efficiently Inhibit Cellular Inhibitor of Apoptosis for Cancer Immunotherapy.