Monitoring the permeability of the nuclear envelope during the cell cycle.
AuthorsLénárt P, Ellenberg J,
JournalMethods
PubMed ID16343937
'In animal organisms the nuclear envelope (NE) dis-assembles during cell division resulting in complete intermixing of cytoplasmic and nuclear compartments. This leads to the activation of many mitotic enzymes, which were kept away from their substrates or regulators by nuclear or cytoplasmic sequestration in interphase. Nuclear envelope breakdown (NEBD) is ... More
Fluorescence correlation microscopy of cells in the presence of autofluorescence.
AuthorsBrock R, Hink MA, Jovin TM
JournalBiophys J
PubMed ID9788950
'Fluorescence correlation microscopy (FCM), the combination of fluorescence correlation spectroscopy (FCS) and digital microscopy (Brock and Jovin, 1998. Cell. Mol. Biol. 44:847-856), has been implemented for measuring molecular diffusion and association in living cells with explicit consideration of autocorrelations arising from autofluorescence. Autofluorescence excited at 532 nm colocalizes with mitochondria, ... More
Near-infrared fluorescence lifetime assay for serum glucose based on allophycocyanin-labeled concanavalin A.
AuthorsMcCartney LJ, Pickup JC, Rolinski OJ, Birch DJ
JournalAnal Biochem
PubMed ID11355853
We describe an assay scheme for glucose based on fluorescence resonance energy transfer (FRET) between concanavalin A (con A), labeled with the near-infrared fluorescent protein allophycocyanin (APC) as donor, and dextran labeled with malachite green (MG) as acceptor. Glucose competitively displaces dextran-MG and leads to reduction in FRET, assessed by ... More
Electrochemiluminescence enzyme immunoassays for TNT and pentaerythritol tetranitrate.
AuthorsWilson R, Clavering C, Hutchinson A
JournalAnal Chem
PubMed ID14632142
Electrochemiluminescence enzyme immunoassays for 2,4,6-trinitrotoluene (TNT) and pentaerythritol tetranitrate (PETN) are described. The latter is, to the best of our knowledge, the first report of an immunoassay for PETN. Haptens corresponding to these explosives were covalently attached to high-affinity dextran-coated paramagnetic beads. The beads were mixed with the corresponding Fab ... More
Hedgehog Pathway Activation Requires Coreceptor-Catalyzed, Lipid-Dependent Relay of the Sonic Hedgehog Ligand.