A dynamic ubiquitin equilibrium couples proteasomal activity to chromatin remodeling.
AuthorsDantuma NP, Groothuis TA, Salomons FA, Neefjes J
JournalJ Cell Biol
PubMed ID16606690
'Protein degradation, chromatin remodeling, and membrane trafficking are critically regulated by ubiquitylation. The presence of several coexisting ubiquitin-dependent processes, each of crucial importance to the cell, is remarkable. This brings up questions on how the usage of this versatile regulator is negotiated between the different cellular processes. During proteotoxic stress, ... More
Intravital two-photon microscopy for studying the uptake and trafficking of fluorescently conjugated molecules in live rodents.
AuthorsMasedunskas A, Weigert R,
JournalTraffic
PubMed ID18647170
'In this study, we describe an experimental system based on intravital two-photon microscopy for studying endocytosis in live animals. The rodent submandibular glands were chosen as model organs because they can be exposed easily, imaged without compromising their function and, furthermore, they are amenable to pharmacological and genetic manipulations. We ... More
An epithelial cell destined for apoptosis signals its neighbors to extrude it by an actin- and myosin-dependent mechanism.
AuthorsRosenblatt J, Raff MC, Cramer LP
JournalCurr Biol
PubMed ID11728307
'BACKGROUND: Simple epithelia encase developing embryos and organs. Although these epithelia consist of only one or two layers of cells, they must provide tight barriers for the tissues that they envelop. Apoptosis occurring within these simple epithelia could compromise this barrier. How, then, does an epithelium remove apoptotic cells without ... More
Intracellular localization and metabolism of chylomicron remnants in the livers of low density lipoprotein receptor-deficient mice and apoE-deficient mice. Evidence for slow metabolism via an alternative apoE-dependent pathway.
'The metabolism of chylomicron remnants in mice deficient in low density lipoprotein receptor (LDLr) or apolipoprotein E (apoE) was compared with that of control C57BL/6J mice. Mice were injected intravenously with chylomicron-like emulsions labeled with radioactive lipids. Blood samples were taken at fixed time intervals from the retro-orbital sinus, and ... More
Lysine-fixable dye tracing of exocytosis shows F-actin coating is a step that follows granule fusion in pancreatic acinar cells.
AuthorsTurvey MR, Thorn P
JournalPflugers Arch
PubMed ID15103465
'Exocytosis, the fusion of a vesicle with the plasma membrane, involves a complex cascade of cellular events. We set out to develop a method to identify changes in the distribution of proteins associated with exocytotic events in secretory epithelial cells. Our model system, the mouse pancreatic acinar cell, contains many ... More
A role for phosphoinositide 3-kinase in the completion of macropinocytosis and phagocytosis by macrophages.
AuthorsAraki N, Johnson MT, Swanson JA
JournalJ Cell Biol
PubMed ID8947549
'Phosphoinositide 3-kinase (PI 3-kinase) has been implicated in growth factor signal transduction and vesicular membrane traffic. It is thought to mediate the earliest steps leading from ligation of cell surface receptors to increased cell surface ruffling. We show here that inhibitors of PI 3-kinase inhibit endocytosis in macrophages, not by ... More
The Canonical Wnt Pathway Drives Macropinocytosis in Cancer.
Authors
JournalCancer Res
PubMed ID29871936
Podocalyxin is required for maintaining blood-brain barrier function during acute inflammation.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID30787191
Pharmacological depletion of microglia and perivascular macrophages prevents Vascular Cognitive Impairment in Ang II-induced hypertension.
Authors
JournalTheranostics
PubMed ID32863942
Longitudinal functional imaging of VIP interneurons reveals sup-population specific effects of stroke that are rescued with chemogenetic therapy.
Authors
JournalNat Commun
PubMed ID34671051
G4C2 Repeat RNA Initiates a POM121-Mediated Reduction in Specific Nucleoporins in C9orf72 ALS/FTD.
Authors
JournalNeuron
PubMed ID32673563
Direct vascular contact is a hallmark of cerebral astrocytes.
Authors
JournalCell Rep
PubMed ID35385728
Mitochondria-Endoplasmic Reticulum Contacts in Reactive Astrocytes Promote Vascular Remodeling.
Authors
JournalCell Metab
PubMed ID32220306
Imaging the construction of capillary networks in the neonatal mouse brain.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID34172585
Calcium Influx through Plasma-Membrane Nanoruptures Drives Axon Degeneration in a Model of Multiple Sclerosis.
Authors
JournalNeuron
PubMed ID30686733
Multicolor two-photon imaging of in vivo cellular pathophysiology upon influenza virus infection using the two-photon IMPRESS.