Analysis of the growth cone turning assay for studying axon guidance.
AuthorsPujic Z, Giacomantonio CE, Unni D, Rosoff WJ, Goodhill GJ,
JournalJ Neurosci Methods
PubMed ID18313760
The
Diffusion of microinjected markers across the parasitophorous vacuole membrane in cells infected with Eimeria nieschulzi (Coccidia, Apicomplexa).
AuthorsWerner-Meier R, Entzeroth R
JournalParasitol Res
PubMed ID9211514
Cells infected with the intracellular parasite Eimeria nieschulzi were microinjected with lucifer yellow (457 Da), biocytin lucifer yellow (850 Da) and dextranrhodamine (10,000 Da). Immediately after injection of a mixture of the markers into the host cell cytoplasm, a differential diffusion pattern was observed in trophozoites and schizonts. Lucifer yellow ... More
Monitoring autophagy in lysosomal storage disorders.
AuthorsRaben N, Shea L, Hill V, Plotz P,
JournalMethods Enzymol
PubMed ID19216919
Lysosomes are the final destination of the autophagic pathway. It is in the acidic milieu of the lysosomes that autophagic cargo is metabolized and recycled. One would expect that diseases with primary lysosomal defects would be among the first systems in which autophagy would be studied. In reality, this is ... More
Neural organization and visual processing in the anterior optic tubercle of the honeybee brain.
AuthorsMota T, Yamagata N, Giurfa M, Gronenberg W, Sandoz JC,
JournalJ Neurosci
PubMed ID21832175
The honeybee Apis mellifera represents a valuable model for studying the neural segregation and integration of visual information. Vision in honeybees has been extensively studied at the behavioral level and, to a lesser degree, at the physiological level using intracellular electrophysiological recordings of single neurons. However, our knowledge of visual ... More
Persistence of gap junction communication during myocardial ischemia.
AuthorsRuiz-Meana M, Garcia-Dorado D, Lane S, Pina P, Inserte J, Mirabet M, Soler-Soler J
JournalAm J Physiol Heart Circ Physiol
PubMed ID11356611
During myocardial ischemia, severe ATP depletion induces rigor contracture followed by intracellular Ca2+ concentration ([Ca2+]i) rise and progressive impairment of gap junction (GJ)-mediated electrical coupling. Our objective was to investigate whether chemical coupling through GJ allows propagation of rigor in cardiomyocytes and whether it persists after rigor development. In end-to-end ... More
Embryo microinjection of the lecithotrophic sea urchin Heliocidaris erythrogramma.
Authors
JournalJ Biol Methods
PubMed ID31772951
In vivo recordings of GnRH neuron firing reveal heterogeneity and dependence upon GABAA receptor signaling.
Authors
JournalJ Neurosci
PubMed ID23719807
Rumba and Haus3 are essential factors for the maintenance of hematopoietic stem/progenitor cells during zebrafish hematopoiesis.
Authors
JournalDevelopment
PubMed ID21228005
RAS-Driven Macropinocytosis of Albumin or Dextran Reveals Mutation-Specific Target Engagement of RAS p.G12C Inhibitor ARS-1620 by NIR-Fluorescence Imaging.
Authors
JournalMol Imaging Biol
PubMed ID34905147
NEK1-mediated retromer trafficking promotes blood-brain barrier integrity by regulating glucose metabolism and RIPK1 activation.
Authors
JournalNat Commun
PubMed ID34376696
Microfluidic jet injection for delivering macromolecules into cells.
Authors
JournalJ Micromech Microeng
PubMed ID23956498
Endothelial Cdk5 deficit leads to the development of spontaneous epilepsy through CXCL1/CXCR2-mediated reactive astrogliosis.
Authors
JournalJ Exp Med
PubMed ID31699822
Distorted Coarse Axon Targeting and Reduced Dendrite Connectivity Underlie Dysosmia after Olfactory Axon Injury.
Authors
JournalEneuro
PubMed ID27785463
Gradual Suppression of Transcytosis Governs Functional Blood-Retinal Barrier Formation.