Biotin-dextran: fast retrograde tracing of sciatic nerve motoneurons.
AuthorsTodorova N, Rodziewicz GS
JournalJ Neurosci Methods
PubMed ID8618412
We present evidence that biotin-dextran (BD) provides good fast retrograde tracing in the rat sciatic nerve. Using BD injected distal to a crush injury of either tibial or common peroneal nerves, spinal cord motoneuron counts after 48 h compare favorably with counts obtained using horseradish peroxidase. Advantages of BD include ... More
Niemann-Pick C1 functions in regulating lysosomal amine content.
AuthorsKaufmann AM, Krise JP,
JournalJ Biol Chem
PubMed ID18591242
'Mutations in the late endosomal/lysosomal membrane protein Niemann-Pick C1 (NPC1) are known to cause a generalized block in retrograde vesicle-mediated transport, resulting in the hyper-accumulation of multiple lysosomal cargos. An important, yet often overlooked, category of lysosomal cargo includes the vast array of small molecular weight amine-containing molecules that are ... More
Versatile, high-resolution anterograde labeling of vagal efferent projections with dextran amines.
'None of the anterograde tracers used to label and investigate vagal preganglionic neurons projecting to the viscera has proved optimal for routine and extensive labeling of autonomic terminal fields. To identify an alternative tracer protocol, the present experiment evaluated whether dextran conjugates, which have produced superior results in the CNS, ... More
The anterograde neuroanatomical tracer biotinylated dextran-amine: comparison with the tracer Phaseolus vulgaris-leucoagglutinin in preparations for electron microscopy.
AuthorsWouterlood FG, Jorritsma-Byham B
JournalJ Neurosci Methods
PubMed ID7690870
'We investigated the properties of biotinylated dextran-amine (BDA) as a neuroanatomical tracer at the electron microscopic level and we compared the results with those obtained previously with another tracer, the lectin Phaseolus vulgaris-leucoagglutinin (PHA-L). BDA was injected into various brain areas of rats. Following survival and fixation, vibratome sections were ... More
Gap junctional communication in the early Xenopus embryo.
AuthorsLandesman Y, Goodenough DA, Paul DL
JournalJ Cell Biol
PubMed ID10953017
'In the Xenopus embryo, blastomeres are joined by gap junctions that allow the movement of small molecules between neighboring cells. Previous studies using Lucifer yellow (LY) have reported asymmetries in the patterns of junctional communication suggesting involvement in dorso-ventral patterning. To explore that relationship, we systematically compared the transfer of ... More
The connection from cortical area V1 to V5: a light and electron microscopic study.
AuthorsAnderson JC, Binzegger T, Martin KA, Rockland KS
JournalJ Neurosci
PubMed ID9852590
'Area V5 (middle temporal) in the superior temporal sulcus of macaque receives a direct projection from the primary visual cortex (V1). By injecting anterograde tracers (biotinylated dextran and Phaseolus vulgaris lectin) into V1, we have examined the synaptic boutons that they form in V5 in the electron microscope. Nearly 80% ... More
An anterograde and retrograde tract-tracing study on the projections from the thalamic gustatory area in the rat: distribution of neurons projecting to the insular cortex and amygdaloid complex.
AuthorsNakashima M, Uemura M, Yasui K, Ozaki HS, Tabata S, Taen A
JournalNeurosci Res
PubMed ID10771108
'Projections from the thalamic gustatory nucleus, i.e. the parvicellular part of the posteromedial ventral thalamic nucleus (VPMpc) to the forebrain regions were studied in the rat by the tract-tracing methods with anterograde tracer (biotinylated dextran amine, BDA) and anterograde/retrograde tracer (wheat-germ agglutinin-horseradish peroxidase, WGA-HRP). After BDA injection into the VPMpc, ... More
Connections of the caudal ventrolateral medullary reticular formation in the cat brainstem.
'A region of the caudal ventrolateral medullary reticular formation (CVLM) participates in baroreceptor, vestibulosympathetic, and somatosympathetic reflexes; the adjacent retroambigual area is involved in generating respiratory-related activity and is essential for control of the upper airway during vocalization. However, little is known about the connections of the CVLM in the ... More
Biotinylated dextran amine as an anterograde tracer for single- and double-labeling studies.
AuthorsVeenman CL, Reiner A, Honig MG
JournalJ Neurosci Methods
PubMed ID1381034
'Fluorescent dextran amines have recently been reported to be useful for anterograde pathway tracing. However, fluorescent markers are not always ideal for detailed mapping studies. We therefore evaluated the efficacy of a biotinylated dextran amine (BDA) for anterograde labeling in several different preparations. BDA was visualized with an avidin-biotinylated HRP ... More
Striatal indirect pathway contributes to selection accuracy of learned motor actions.
AuthorsNishizawa K, Fukabori R, Okada K, Kai N, Uchigashima M, Watanabe M, Shiota A, Ueda M, Tsutsui Y, Kobayashi K,
JournalJ Neurosci
PubMed ID23015433
'The dorsal striatum, which contains the dorsolateral striatum (DLS) and dorsomedial striatum (DMS), integrates the acquisition and implementation of instrumental learning in cooperation with the nucleus accumbens (NAc). The dorsal striatum regulates the basal ganglia circuitry through direct and indirect pathways. The mechanism by which these pathways mediate the learning ... More
Motor recovery and anatomical evidence of axonal regrowth in spinal cord-repaired adult rats.
AuthorsLee YS, Lin CY, Robertson RT, Hsiao I, Lin VW
JournalJ Neuropathol Exp Neurol
PubMed ID15055447
'Behavioral assessments of hindlimb motor recovery and anatomical assessments of extended axons of long spinal tracts were conducted in adult rats following complete spinal cord transection. Rats were randomly divided into 3 groups: 1) sham control group (laminectomy only; n = 12); 2) transection-only group, spinal cord transection at T8 ... More
Electron microscopic analysis of D1 and D2 dopamine receptor proteins in the dorsal striatum and their synaptic relationships with motor corticostriatal afferents.
AuthorsHersch SM, Ciliax BJ, Gutekunst CA, Rees HD, Heilman CJ, Yung KK, Bolam JP, Ince E, Yi H, Levey AI
JournalJ Neurosci
PubMed ID7623147
'The precise localization of D1 and D2 dopamine receptors within striatal neurons and circuits is crucial information for further understanding dopamine pharmacology. We have used subtype specific polyclonal and monoclonal antibodies against D1 and D2 dopamine receptors to determine their cellular and subcellular distributions, their colocalization, and their differential connectivity ... More
Spinovestibular projections in the rat, with particular reference to projections from the central cervical nucleus to the lateral vestibular nucleus.
AuthorsMatsushita M, Gao X, Yaginuma H
JournalJ Comp Neurol
PubMed ID8543666
'Projections from the spinal cord to the vestibular nuclei were examined following injections of Phaseolus vulgaris-leucoagglutinin, cholera toxin subunit B, or biotinylated dextran at various levels of the spinal cord in the rat. Labeled terminals were abundant after injections of the tracers into the C2 and C3 segments containing the ... More
Comparison of wheat germ agglutinin-horseradish peroxidase and biotinylated dextran for anterograde tracing of corticospinal tract following spinal cord injury.
AuthorsFerguson IA, Xian C, Barati E, Rush RA
JournalJ Neurosci Methods
PubMed ID11513942
'Established methods for monitoring regeneration of the corticospinal tract involve anterograde labelling of the cortical motor neuron. While wheat germ agglutinin-horseradish peroxidase conjugate has been used to anterogradely label these neurons, we demonstrate that this technique may not completely label the whole axon and fine terminal processes when this tracer ... More
Axonal collateral-collateral transport of tract tracers in brain neurons: false anterograde labelling and useful tool.
AuthorsChen S, Aston-Jones G
JournalNeuroscience
PubMed ID9466437
'It is well established that some neuroanatomical tracers may be taken up by local axonal terminals and transported to distant axonal collaterals (e.g., transganglionic transport in dorsal root ganglion cells). However, such collateral-collateral transport of tracers has not been systematically examined in the central nervous system. We addressed this issue ... More
Thalamic collaterals of corticostriatal axons: their termination field and synaptic targets in cats.
AuthorsParé D, Smith Y
JournalJ Comp Neurol
PubMed ID8876453
'Branched cortical projections to the thalamus and striatum were investigated in cats by injecting the retrograde-anterograde tracer biotinylated-dextran amine (BDA) into the caudate nucleus. These injections gave rise to plexuses of labeled fibers and varicosities in widespread thalamic territories. For instance, the lateroposterior nucleus and pulvinar (LP-PUL) mostly contained thick ... More
Evidence for differential cortical input to direct pathway versus indirect pathway striatal projection neurons in rats.
AuthorsLei W, Jiao Y, Del Mar N, Reiner A,
JournalJ Neurosci
PubMed ID15385612
'The two main types of corticostriatal neurons are those that project only intratelencephalically (IT-type), the intrastriatal terminals of which are 0.41 microm in mean diameter, and those that send their main axon into pyramidal tract and have a collateral projection to striatum (PT-type), the intrastriatal terminals of which are 0.82 ... More
Eph tyrosine kinase receptor EphA4 is required for the topographic mapping of the corticospinal tract.
AuthorsCanty AJ, Greferath U, Turnley AM, Murphy M
JournalProc Natl Acad Sci U S A
PubMed ID17030822
'Fine movement in the body is controlled by the motor cortex, which signals in a topographically specific manner to neurons in the spinal cord by means of the corticospinal tract (CST). How the correct topography of the CST is established is unknown. To investigate the possibility that the Eph tyrosine ... More
Telencephalic connections of the visual system of the chicken: tracing the interrelation of the efferents of the visual Wulst and the hyperstriatum ventrale.
AuthorsAlpár A, Tömböl T
JournalAnn Anat
PubMed ID9862033
'In the present study the telencephalic connections between the centres of the tectofugal and thalamofugal pathways were investigated with Phaseolus vulgaris leucoagglutinin and biotinylated dextran-amine anterograde tracers in chicken using light and electron microscopy. No direct connection was found between the visual Wulst and the ectostriatum or the telencephalic centres ... More
Complex brain circuits studied via simultaneous and permanent detection of three transported neuroanatomical tracers in the same histological section.
AuthorsLanciego JL, Wouterlood FG, Erro E, Arribas J, Gonzalo N, Urra X, Cervantes S, Giménez-Amaya JM
JournalJ Neurosci Methods
PubMed ID11074102
'Experimental neuroanatomical tracing methods lie at the basis of the study of the nervous system. When the scientific question is relatively straightforward, it may be sufficient to derive satisfactory answers from experiments in which a single neuroanatomical tracing method is applied. In various scientific paradigms however, for instance when the ... More
A fluorescence resonance energy transfer-based approach for investigating late endosome-lysosome retrograde fusion events.
AuthorsKaufmann AM, Goldman SD, Krise JP,
JournalAnal Biochem
PubMed ID19109922
'Traditionally, lysosomes have been considered to be a terminal endocytic compartment. Recent studies suggest that lysosomes are quite dynamic, being able to fuse with other late endocytic compartments as well as with the plasma membrane. Here we describe a quantitative fluorescence energy transfer (FRET)-based method for assessing rates of retrograde ... More
Ensheathing cells and methylprednisolone promote axonal regeneration and functional recovery in the lesioned adult rat spinal cord.
AuthorsNash HH, Borke RC, Anders JJ
JournalJ Neurosci
PubMed ID12177207
'Axons fail to regenerate after spinal cord injury (SCI) in adult mammals, leading to permanent loss of function. After SCI, ensheathing cells (ECs) promote recovery in animal models, whereas methylprednisolone (MP) promotes neurological recovery in humans. In this study, the effectiveness of combining ECs and MP after SCI was investigated ... More
Efferent projections of the retrorubral nucleus to the substantia nigra and ventral tegmental area in cats as shown by anterograde tracing.
AuthorsArts MP, Groenewegen HJ, Veening JG, Cools AR
JournalBrain Res Bull
PubMed ID8736584
'The aim of the present study was to determine whether the retrorubral nucleus projects to the dopaminergic nuclei in the ventral midbrain of the cat. For this purpose, injections of biotinylated dextran-amine or Phaseolus vulgaris-leucoagglutinin were placed into the retrorubral nucleus under stereotaxic guidance. The tracers were visualized by means ... More
Projections from the primary auditory cortex onto the dorsal cortex of the inferior colliculus in albino rats.
AuthorsHerrera M, Hurtado-García JF, Collia F, Lanciego J
JournalArch Ital Biol
PubMed ID7526815
'The topography of connections from the primary auditory cortex (Te1) onto the dorsal cortex (DC) of the inferior colliculus (IC) was studied using the anterograde neural tracers Phaseolus vulgaris (PHA-L) and Biotinylated dextran amine (BDA). Injections in different restricted portions of the ventral Te1 showed labelled axonal sheets in the ... More
Non-serotonergic dorsal and median raphe projection onto parvalbumin- and calbindin-containing neurons in hippocampus and septum.
AuthorsAznar S, Qian ZX, Knudsen GM
JournalNeuroscience
PubMed ID14980728
'The median raphe nucleus is involved in controlling and maintaining hippocampal activity through its projection to inhibitory neurons in medial septum and hippocampus. It has been shown that anterogradely axonal-traced fibers originating in the median raphe nucleus project onto calbindin-containing neurons in hippocampus and parvalbumin-containing neurons in medial septum. Parallel ... More
Glutamatergic pathways from the Kölliker-Fuse nucleus to the phrenic nucleus in the rat.
AuthorsYokota S, Tsumori T, Ono K, Yasui Y
JournalBrain Res
PubMed ID14644477
'After ipsilateral injections of biotinylated dextran amine (BDA) into the Kölliker-Fuse (KF) nucleus and cholera toxin B subunit (CTb) into the ventral horn in C4 to C5 segments of the spinal cord, an overlapping distribution of BDA-labeled axon terminals and CTb-labeled neurons was found in the rostral ventral respiratory group ... More
Identification of two distinct mechanisms of phagocytosis controlled by different Rho GTPases.
AuthorsCaron E, Hall A
JournalScience
PubMed ID9831565
'The complement and immunoglobulin receptors are the major phagocytic receptors involved during infection. However, only immunoglobulin-dependent uptake results in a respiratory burst and an inflammatory response in macrophages. Rho guanosine triphosphatases (molecular switches that control the organization of the actin cytoskeleton) were found to be essential for both types of ... More
Trigeminal projections to hypoglossal and facial motor nuclei in the rat.
AuthorsPinganaud G, Bernat I, Buisseret P, Buisseret-Delmas C
JournalJ Comp Neurol
PubMed ID10540360
'This study was undertaken to identify the trigeminal nuclear regions connected to the hypoglossal (XII) and facial (VII) motor nuclei in rats. Anterogradely transported tracers (biotinylated dextran amine, biocytin) were injected into the various subdivisions of the sensory trigeminal complex, and labeled fibers and terminals were searched for in the ... More
Multiple axonal tracing: simultaneous detection of three tracers in the same section.
AuthorsLanciego JL, Wouterlood FG, Erro E, Giménez-Amaya JM
JournalHistochem Cell Biol
PubMed ID9826130
'Multiple neuroanatomical tract-tracing methods are important tools for elucidating the connectivity between different populations of neurons. Evaluation of the question as to whether two specific fiber inputs converge on a particular, identified population of projection neurons requires the application of a triple-staining procedure that allows the unequivocal detection of three ... More
Biotin-dextran: a sensitive anterograde tracer for neuroanatomic studies in rat and monkey.
AuthorsBrandt HM, Apkarian AV
JournalJ Neurosci Methods
PubMed ID1283432
The properties of a new anterograde tracer, biotin-dextran (BD), are reported. Iontophoretic or pressure injections of BD in the spinal cord of rats and monkeys revealed terminal-like BD label in many regions of the brain. The BD label was visualized by an avidin-biotin reaction combined with diaminobenzidine, with and without ... More
A sequential protocol combining dual neuroanatomical tract-tracing with the visualization of local circuit neurons within the striatum.
AuthorsGonzalo N, Moreno A, Erdozain MA, García P, Vázquez A, Castle M, Lanciego JL
JournalJ Neurosci Methods
PubMed ID11574120
We describe here an experimental approach designed to aid in the identification of complex brain circuits within the rat corpus striatum. Our aim was to characterize in a single section (i) striatal thalamic afferents, (ii) striatopallidal projection neurons and (iii) striatal local circuit interneurons. To this end, we have combined ... More
Early development of ocular dominance columns.
AuthorsCrowley JC, Katz LC
JournalScience
PubMed ID11082053
The segregation of lateral geniculate nucleus (LGN) axons into ocular dominance columns is believed to involve a prolonged, activity-dependent sorting process. However, visualization of early postnatal ferret LGN axons by direct LGN tracer injections revealed segregated ocular dominance columns <7 days after innervation of layer 4. These early columns were ... More
A monoclonal antibody specific to a song system nuclear antigen in estrildine finches.
AuthorsAkutagawa E, Konishi M
JournalNeuron
PubMed ID11545714
This paper describes a monoclonal antibody that recognizes a molecule whose expression is mostly restricted to some of the forebrain areas that control singing behavior in adult estrildine species studied, including the zebra, Bengalese, and spice finches. When the song system displays extreme sexual dimorphism, as in these species, antibody ... More
Somatosensory projections to cochlear nucleus are upregulated after unilateral deafness.
AuthorsZeng C, Yang Z, Shreve L, Bledsoe S, Shore S,
JournalJ Neurosci
PubMed ID23136418
The cochlear nucleus (CN) receives innervation from auditory and somatosensory structures, which can be identified using vesicular glutamate transporters, VGLUT1 and VGLUT2. VGLUT1 is highly expressed in the magnocellular ventral CN (VCN), which receives auditory nerve inputs. VGLUT2 is predominantly expressed in the granule cell domain (GCD), which receives nonauditory ... More
Two-color fluorescence labeling in acrolein-fixed brain tissue.
AuthorsLuquin E, Pérez-Lorenzo E, Aymerich MS, Mengual E,
JournalJ Histochem Cytochem
PubMed ID20051381
Acrolein is a potent fixative that provides both excellent preservation of ultrastructural morphology and retention of antigenicity, thus it is frequently used for immunocytochemical detection of antigens at the electron microscopic level. However, acrolein is not commonly used for fluorescence microscopy because of concerns about possible autofluorescence and destruction of ... More
A proposal for a coordinated effort for the determination of brainwide neuroanatomical connectivity in model organisms at a mesoscopic scale.
AuthorsBohland JW, Wu C, Barbas H, Bokil H, Bota M, Breiter HC, Cline HT, Doyle JC, Freed PJ, Greenspan RJ, Haber SN, Hawrylycz M, Herrera DG, Hilgetag CC, Huang ZJ, Jones A, Jones EG, Karten HJ, Kleinfeld D, Kötter R, Lester HA, Lin JM, Mensh BD, Mikula S, Panksepp J, Price JL, Safdieh J, Saper CB, Schiff ND, Schmahmann JD, Stillman BW, Svoboda K, Swanson LW, Toga AW, Van Essen DC, Watson JD, Mitra PP,
JournalPLoS Comput Biol
PubMed ID19325892
In this era of complete genomes, our knowledge of neuroanatomical circuitry remains surprisingly sparse. Such knowledge is critical, however, for both basic and clinical research into brain function. Here we advocate for a concerted effort to fill this gap, through systematic, experimental mapping of neural circuits at a mesoscopic scale ... More
Giant neurons in the macaque pulvinar: a distinct relay subpopulation.
AuthorsImura K, Rockland KS,
JournalFront Neuroanat
PubMed ID18958196
Calbindin positive (CB+) giant neurons are known to occur within the pulvinar nucleus in subhuman primates. Here, we demonstrate by combined retrograde tracing and immunocytochemistry that at least some of these are pulvinocortical relay neurons, and further report several distinctive features. First, in contrast with non-giant relay neurons, the giant ... More
Connectivity of neurons in identified auditory circuits studied with transport of dextran and microspheres plus intracellular injection of lucifer yellow.
AuthorsOliver DL, Beckius GE, Ostapoff EM
JournalJ Neurosci Methods
PubMed ID7527475
The components of a neural circuit are usually distinguished in separate experiments to identify long connections, presynaptic, and postsynaptic components. We describe a procedure to visualize these components in the same experiment. Neurons in the inferior colliculus the axons of which project to the medial geniculate body were identified by ... More
Surface biopassivation of replicated poly(dimethylsiloxane) microfluidic channels and application to heterogeneous immunoreaction with on-chip fluorescence detection.
AuthorsLinder V, Verpoorte E, Thormann W, de Rooij NF, Sigrist H
JournalAnal Chem
PubMed ID11569807
Poly(dimethylsiloxane) (PDMS) appeared recently as a material of choice for rapid and accurate replication of polymer-based microfluidic networks. However, due to its hydrophobicity, the surface strongly interacts with apolar analytes or species containing apolar domains, resulting in significant uncontrolled adsorption on channel walls. This contribution describes the application and characterization ... More
Multiplexed DNA quantification by spectroscopic shift of two microsphere cavities.
AuthorsVollmer F, Arnold S, Braun D, Teraoka I, Libchaber A
JournalBiophys J
PubMed ID12944310
We have developed a novel, spectroscopic technique for high-sensitivity, label-free DNA quantification. We demonstrate that an optical resonance (whispering gallery mode) excited in a micron-sized silica sphere can be used to detect and measure nucleic acids. The surface of the silica sphere is chemically modified with oligonucleotides. We show that ... More
Projections from the auditory cortex to the superior olivary complex in guinea pigs.
AuthorsCoomes DL, Schofield BR
JournalEur J Neurosci
PubMed ID15090045
We used anterograde tracing techniques to characterize projections from auditory cortex to the superior olivary complex (SOC) in guinea pigs. Large injections of fluorescent or biotinylated dextrans into the temporal cortex labeled many axons in the SOC. Labeled boutons were most numerous in the ventral nucleus of the trapezoid body, ... More
The grasshopper median neuroblast is a multipotent progenitor cell that generates glia and neurons in distinct temporal phases.
AuthorsCondron BG, Zinn K
JournalJ Neurosci
PubMed ID7931543
The midline of the insect CNS provides a good system for studying the control of neuronal and glial cell fates, because it contains a small number of distinct cell types that arise from a unique set of precursors. In this report we analyze the development of the CNS midline in ... More
Vesicular acetylcholine transporter can be a morphological marker for the reinnervation to muscle of regenerating motor axons.
AuthorsMaeda M, Ohba N, Nakagomi S, Suzuki Y, Kiryu-Seo S, Namikawa K, Kondoh W, Tanaka A, Kiyama H
JournalNeurosci Res
PubMed ID15154676
This study was designed to evaluate whether the vesicular acetylcholine transporter (VAChT), which packages acetylcholine into synaptic vesicles, can be used as a marker for regenerating motor axon terminal. We examined motor axon regeneration in the tongue after hypoglossal nerve axotomy, using an anterograde tracer biotin-dextran (BD), retrograde tracer Fluoro-Gold ... More
Improved Golgi-like visualization in retrogradely projecting neurons after EGFP-adenovirus infection in adult rat and monkey.
AuthorsTomioka R, Rockland KS
JournalJ Histochem Cytochem
PubMed ID16344324
An adenovirus vector was generated using a neuron-specific promoter synapsin I and enhanced green fluorescent protein (EGFP) reporter (AdSynEGFP). In addition, two modifications were identified that resulted in robust and reliable retrograde transport and EGFP expression after injection of the virus into three different brain regions in adult rats (medial ... More
Combined anterograde tracing with biotinylated dextran-amine, retrograde tracing with fast blue and intracellular filling of neurons with lucifer yellow: an electron microscopic method.
In order to determine the presence of synaptic connectivity between fibres originating from a specific source and neurones with a known morphology and known fibre projection, we have introduced a method for electron microscopy that combines three techniques: retrograde fluorescent tracing, anterograde tracing using biotinylated dextran-amine and intracellular injection of ... More
Connections of the pallial telencephalon in the Senegal bichir, Polypterus.
AuthorsHolmes PH, Northcutt RG
JournalBrain Behav Evol
PubMed ID12697955
The connections of the dorsal telencephalon (pallium) of the Senegal bichir (Polypterus senegalus) were investigated in order to test the hypothesis that the simple everted pallium of polypterid fishes is directly comparable to the evaginated pallium of most vertebrates. Neuroanatomical tracers (DiI or biotinylinated dextran amines) were injected into the ... More
Electron microscopic analysis of synaptic inputs from the median preoptic nucleus and adjacent regions to the supraoptic nucleus in the rat.
AuthorsArmstrong WE, Tian M, Wong H
JournalJ Comp Neurol
PubMed ID8889924
The median preoptic nucleus (MnPo) is critical for normal fluid balance, mediating osmotically evoked drinking and neurohypophysial hormone secretion. The influence of the MnPo on vasopressin and oxytocin release is in part through direct connections to the supraoptic and paraventricular nucleus. In the present investigation the synaptic contacts between the ... More
Direct input from cochlear root neurons to pontine reticulospinal neurons in albino rat.
AuthorsNodal FR, López DE
JournalJ Comp Neurol
PubMed ID12687698
The cochlear root neurons (CRNs) are thought to mediate the auditory startle reflex (ASR) in the rat, which is widely used as a behavioral model for the investigation of the sensorimotor integration. CRNs project, among other targets, to the nucleus reticularis pontis caudalis (PnC), a major component of the ASR ... More
Quantitative comparisons of corticothalamic topography within the ventrobasal complex and the posterior nucleus of the rodent thalamus.
AuthorsAlloway KD, Hoffer ZS, Hoover JE
JournalBrain Res
PubMed ID12644264
To compare the topographic precision of corticothalamic projections to the ventrobasal (VB) complex and the medial part of the posterior (POm) complex, different anterograde tracers were placed in neighboring parts of the primary (SI) and secondary (SII) somatosensory cortical areas. The location of labeled corticothalamic terminals and their beaded varicosities ... More
Direct parabrachial nuclear projections to the pharyngeal motoneurons in the rat: an anterograde and retrograde double-labeling study.
AuthorsHayakawa T, Zheng JQ, Seki M
JournalBrain Res
PubMed ID9878830
The parabrachial nucleus consists of several subnuclei which contains autonomic, gustatory, visceral sensory, nociceptive, and respiratory related neurons. We have investigated the direct projections from the rat parabrachial region, including the K olliker-Fuse nucleus, to the pharyngeal motoneurons with an anterograde and retrograde double-tracing technique. The cholera toxin subunit-B was ... More
Origin of noradrenergic afferents to the shell subregion of the nucleus accumbens: anterograde and retrograde tract-tracing studies in the rat.
AuthorsDelfs JM, Zhu Y, Druhan JP, Aston-Jones GS
JournalBrain Res
PubMed ID9739125
The nucleus accumbens (NAcc) can be subdivided into 'core' and 'shell' based on anatomical connections and histochemical markers. Previous studies have demonstrated dopamine-beta-hydroxylase immunoreactive (DBH-ir) fibers in the NAcc shell, but the source of these noradrenergic (NE) afferents has not been determined. Therefore, we have investigated in detail the anatomy ... More
Patterning of local intracortical projections within the vibrissae representation of rat primary somatosensory cortex.
AuthorsHoeflinger BF, Bennett-Clarke CA, Chiaia NL, Killackey HP, Rhoades RW
JournalJ Comp Neurol
PubMed ID7541807
Anterograde and retrograde tracing with biotinylated dextran amine and Phaseolus vulgaris leukoagglutinin was used to assess projection patterns within the vibrissae representation of the rat's primary somatosensory cortex (S-I). Large and small injections of either tracer into the center of the vibrissae representation yielded dense anterograde and retrograde labelling throughout ... More
A new method for simultaneous demonstration of anterograde and retrograde connections in the brain: co-injections of biotinylated dextran amine and the beta subunit of cholera toxin.
In studying reciprocally connected brain networks, it is advantageous to use techniques that allow simultaneous visualization of both efferent and afferent connections from a single injection site. We report on a new technique to achieve this using pressure injections of a mixture of biotinylated dextran amine (BDA) and the beta ... More
Cortical inputs to m2-immunoreactive striatal interneurons in rat and monkey.
AuthorsThomas TM, Smith Y, Levey AI, Hersch SM
JournalSynapse
PubMed ID10891862
Previous anatomical studies have been unsuccessful in demonstrating significant cortical inputs to cholinergic and somatostatinergic striatal interneurons in rats. On the other hand, electrophysiological studies have shown that cortical stimulation induces monosynaptic EPSPs in cholinergic interneurons. It has been proposed that the negative anatomical findings might have been the result ... More
Thalamocortical and intracortical projections to the forelimb-stump SI representation of rats that sustained neonatal forelimb removal.
AuthorsStojic AS, Lane RD, Killackey HP, Qadri BA, Rhoades RW
JournalJ Comp Neurol
PubMed ID9822148
We previously reported the abnormal expression of hindlimb receptive fields in the stump representation of the primary somatosensory cortex (SI) in rats that sustained neonatal forelimb removal when cortical gamma-aminobutyric acid (GABA) receptors were pharmacologically blocked (Lane et al. [1997] J. Neurophysiol. 77:2723-2735). In this study, we attempted to identify ... More
Connections of some auditory-responsive posterior thalamic nuclei putatively involved in activation of the hypothalamo-pituitary-adrenocortical axis in response to audiogenic stress in rats: an anterograde and retrograde tract tracing study combined with Fos expression.
AuthorsCampeau S, Watson SJ
JournalJ Comp Neurol
PubMed ID10870087
Prior studies in our laboratory demonstrated that part of the thalamus is necessary for activating the hypothalamo-pituitary-adrenocortical (HPA) axis in response to audiogenic stress in rats. The present studies were designed to determine how the auditory-responsive thalamic nuclei might activate the HPA axis. Both retrograde [Fluoro-Gold (FG)] and anterograde [Phasoleus ... More
Vocal-acoustic pathways in a teleost fish.
AuthorsBass AH, Marchaterre MA, Baker R
JournalJ Neurosci
PubMed ID8027760
Many teleost fish generate acoustic signals for vocal communication by the synchronized, high-frequency contraction of skeletal, sonic muscles. In midshipman, eight groups of brainstem neurons were distinguished after biocytin application to the sonic nerve that, we propose, represent the entire vocal motor circuit. Biocytin-filled terminals were ubiquitous within all areas ... More
Differential synaptic innervation of striatofugal neurones projecting to the internal or external segments of the globus pallidus by thalamic afferents in the squirrel monkey.
AuthorsSidibé M, Smith Y
JournalJ Comp Neurol
PubMed ID8822181
It is well established that the centromedian nucleus (CM) is the major source of thalamic afferents to the sensorimotor territory of the striatum in monkeys. However, the projection sites of striatal neurons contacted by thalamic afferents still remain to be determined. We therefore carried out an anatomical study aimed at ... More
Entorhinal cortex of the rat: organization of intrinsic connections.
AuthorsDolorfo CL, Amaral DG
JournalJ Comp Neurol
PubMed ID9703027
Two sets of experiments were carried out to examine the organization of associational connections within the rat entorhinal cortex. First, a comprehensive analysis of the areal and laminar distribution of intrinsic projections was performed by using the anterograde tracers Phaseolus vulgaris-leuocoagglutinin (PHA-L) and biotinylated dextran amine (BDA). Second, retrograde tracers ... More
Nigral innervation of cholinergic and glutamatergic cells in the rat mesopontine tegmentum: light and electron microscopic anterograde tracing and immunohistochemical studies.
AuthorsGrofova I, Zhou M
JournalJ Comp Neurol
PubMed ID9596529
The substantia nigra (SN) has long been known as an important source of afferents to the pedunculopontine tegmental nucleus (PPN). However, it has not been established which of the chemospecific cell populations receive this synaptic input. We sought to address this issue by a correlative light and electron microscopic approach ... More
Synaptic integration of functionally diverse pallidal information in the entopeduncular nucleus and subthalamic nucleus in the rat.
AuthorsBevan MD, Clarke NP, Bolam JP
JournalJ Neurosci
PubMed ID8987757
To determine the principles of synaptic innervation of neurons in the entopeduncular nucleus and subthalamic nucleus by neurons of functionally distinct regions of the pallidal complex, double anterograde labeling was carried out at both light and electron microscopic levels in the rat. Deposits of the anterograde tracers Phaseolus vulgaris-leucoagglutinin and ... More
Organization of the disynaptic pathway from the anteroventral cochlear nucleus to the lateral superior olivary nucleus in the ferret.
AuthorsHenkel CK, Gabriele ML
JournalAnat Embryol (Berl)
PubMed ID9930621
The medial nucleus of the trapezoid body (MNTB) is one of three major nuclei of the superior olivary complex and provides an important inhibitory input from the contralateral ear to the lateral superior olivary nucleus (LSO) in the initial binaural pathway for coding interaural intensity differences. The major input to ... More
Selective anterograde tracing of the individual serotonergic and nonserotonergic components of the dorsal raphe nucleus projection to the vestibular nuclei.
AuthorsHalberstadt AL, Balaban CD
JournalNeuroscience
PubMed ID17507165
It is well known that the dorsal raphe nucleus (DRN) sends serotonergic and nonserotonergic projections to target regions in the brain stem and forebrain, including the vestibular nuclei. Although retrograde tracing studies have reported consistently that there are differences in the relative innervation of different target regions by serotonergic and ... More
Evidence for a possible avian dorsal thalamic region comparable to the mammalian ventral anterior, ventral lateral, and oral ventroposterolateral nuclei.
AuthorsMedina L, Veenman CL, Reiner A
JournalJ Comp Neurol
PubMed ID9214542
In the present study, we investigated whether a dorsal thalamic region comparable to the motor part of the mammalian ventral tier (the ventral anterior nucleus, the ventral lateral nucleus, and the oral ventroposterolateral nucleus) exists in pigeon. With this aim, we reinvestigated the projections of the pigeon dorsal pallidum to ... More
Intra- and inter-areal connections between the primary visual cortex V1 and the area immediately surrounding V1 in the rat.
AuthorsRumberger A, Tyler CJ, Lund JS
JournalNeuroscience
PubMed ID11226668
We have qualitatively and quantitatively analysed the anatomical connections within and between rat primary visual cortex (V1) and the rim region surrounding area V1, using both ortho- and retrograde anatomical tracers (biotinylated dextran amine, biocytin, cholera toxin b subunit). From the analysis of the projection patterns, and with the assumption ... More
The subthalamic nucleus and the external pallidum: two tightly interconnected structures that control the output of the basal ganglia in the monkey.
AuthorsShink E, Bevan MD, Bolam JP, Smith Y
JournalNeuroscience
PubMed ID8783253
The aim of the present study was to elucidate the organization of the interconnections between the subthalamic nucleus and the two segments of the globus pallidus in squirrel monkeys. By making small deposits of tracers in the two segments of the globus pallidus, we demonstrate that interconnected neurons of the ... More
Convection-enhanced distribution of large molecules in gray matter during interstitial drug infusion.
Many novel experimental therapeutic agents, such as neurotrophic factors, enzymes, biological modifiers, and genetic vectors, do not readily cross the blood-brain barrier. An effective strategy to deliver these compounds to the central nervous system is required for their application in vivo. Under normal physiological conditions, brain interstitial fluid moves by ... More
Thalamic inputs to striatal interneurons in monkeys: synaptic organization and co-localization of calcium binding proteins.
AuthorsSidibé M, Smith Y
JournalNeuroscience
PubMed ID10362307
Recent studies indicate that extrinsic inputs from sensorimotor regions of the cerebral cortex and the centromedian intralaminar thalamic nucleus terminate preferentially upon specific subpopulations of striatal output neurons in monkeys. The objective of the present study was to verify whether this specificity of innervation also characterizes the synaptic interactions between ... More
Morphological correlates of bilateral synchrony in the rat cerebellar cortex.
AuthorsDe Zeeuw CI, Lang EJ, Sugihara I, Ruigrok TJ, Eisenman LM, Mugnaini E, Llinás R
JournalJ Neurosci
PubMed ID8627376
Simultaneous recordings of the left and right crus IIA of the cerebellar cortex in the rat have demonstrated that Purkinje cells of both sides can be activated synchronously by their climbing fibers. Because climbing fibers arise exclusively from the contralateral inferior olive (IO), this physiological finding seems to contradict the ... More
DOI-Induced activation of the cortex: dependence on 5-HT2A heteroceptors on thalamocortical glutamatergic neurons.
AuthorsScruggs JL, Patel S, Bubser M, Deutch AY
JournalJ Neurosci
PubMed ID11102493
Administration of the hallucinogenic 5-HT(2A/2C) agonist 1-[2, 5-dimethoxy-4-iodophenyl]-2-aminopropane (DOI) induces expression of Fos protein in the cerebral cortex. To understand the mechanisms subserving this action of DOI, we examined the consequences of pharmacological and surgical manipulations on DOI-elicited Fos expression in the somatosensory cortex of the rat. DOI dose-dependently increased ... More
A polysynaptic feedback circuit in rat visual cortex.
AuthorsJohnson RR, Burkhalter A
JournalJ Neurosci
PubMed ID9278547
Feedback connections from extrastriate cortex to primary visual cortex (V1) in the primate may provide "top-down" information that plays a role in visual attention and object recognition. Our work in a rodent model of corticocortical circuitry demonstrates that feedback pathways synapse preferentially with pyramidal cells in V1 () and favor ... More
Projections from primary somatosensory cortex to the neostriatum: the role of somatotopic continuity in corticostriatal convergence.
AuthorsHoover JE, Hoffer ZS, Alloway KD
JournalJ Neurophysiol
PubMed ID12611938
We characterized the organization of corticostriatal projections from rodent primary somatosensory cortex (SI), testing the hypothesis that projections from SI areas representing subcomponents of the forelimb exhibit greater neostriatal overlap than projections from areas representing separate body parts. The anterograde tracers Fluoro-Ruby (FR), Alexa Fluor (AF), and biotinylated dextran amine ... More
Serotonergic innervation of mesencephalic trigeminal nucleus neurons: a light and electron microscopic study in the rat.
AuthorsLi J, Xiong KH, Li YQ, Kaneko T, Mizuno N
JournalNeurosci Res
PubMed ID10867175
Neurons of the mesencephalic trigeminal nucleus (MTN) are considered to be homologous to mechanosensitive neurons in the sensory ganglia. The sites of origin of serotonin (5HT)-immunoreactive axons on neuronal cell bodies in the MTN were studied in the rat by combining immunofluorescence histochemical techniques with retrograde tracing of Fluoro-Gold (FG) ... More
Novel applications of common stereology software to represent the complete distribution, density and spatial organization of anterogradely labelled fibers in neuroanatomical tract-tracing studies.
AuthorsLeite-Almeida H, Almeida A
JournalJ Neurosci Methods
PubMed ID17368789
In a recent study we have adapted for the first time available software used in spatial neuronal organization studies to represent the complete fiber distribution of a brain projection [Leite-Almeida H, Valle-Fernandes A, Almeida A. Brain projections from the medullary dorsal reticular nucleus: an anterograde and retrograde tracing study in ... More
Pathway tracing using biotinylated dextran amines.
AuthorsReiner A, Veenman CL, Medina L, Jiao Y, Del Mar N, Honig MG
JournalJ Neurosci Methods
PubMed ID11074093
Biotinylated dextran amines (BDA) are highly sensitive tools for anterograde and retrograde pathway tracing studies of the nervous system. BDA can be reliably delivered into the nervous system by iontophoretic or pressure injection and visualized with an avidin-biotinylated HRP (ABC) procedure, followed by a standard or metal-enhanced diaminobenzidine (DAB) reaction. ... More
GABAergic neurons in rat nuclei of solitary tracts receive inhibitory-type synapses from amygdaloid efferents lacking detectable GABA-immunoreactivity.
AuthorsPickel VM, Van Bockstaele EJ, Chan J, Cestari DM
JournalJ Neurosci Res
PubMed ID8776666
Gamma-aminobutyric acid (GABA) is a prominent inhibitory transmitter in both the central nucleus of the amygdala (Ce) and the medial nuclei of the solitary tracts (mNTS). These regions are reciprocally connected by anatomical pathways mediating the coordinated visceral responses to emotional stress. To further determine whether GABA is present in ... More
The distribution and origin of the calretinin-containing innervation of the nucleus accumbens of the rat.
AuthorsBubser M, Scruggs JL, Young CD, Deutch AY
JournalEur J Neurosci
PubMed ID10792437
The nucleus accumbens of the rat consists of several subregions that can be distinguished on the basis of histochemical markers. For example, the calcium-binding protein calbindin D28k is a useful marker of the core compartment of the nucleus accumbens. Calretinin, another calcium-binding protein, is found in a dense fibre plexus ... More
Connection from the dorsal column nuclei to the superior colliculus in the rat: topographical organization and somatotopic specific plasticity in response to neonatal enucleation.
AuthorsGarcía Del Caño G, Uria I, Gerrikagoitia I, Martínez-Millán L
JournalJ Comp Neurol
PubMed ID14681934
Somatosensory stimuli from the body to deep and intermediate strata of the superior colliculus (SC) are relayed from the dorsal column nuclei (DCN), gracile (GrN) and cuneate (CuN). Electrophysiological studies have shown that the somatosensory representation in SC is arranged into a map-like pattern. However, there is a lack of ... More
Monosynaptic projections from the medullary gigantocellular reticular formation to sympathetic preganglionic neurons in the thoracic spinal cord.
AuthorsAicher SA, Reis DJ, Nicolae R, Milner TA
JournalJ Comp Neurol
PubMed ID8847418
Microinjection of L-glutamate into a restricted area of the medullary gigantocellular reticular formation, the gigantocellular depressor area (GiDA), lowers arterial pressure. Unlike the nuclei tractus solitarii and the caudal ventrolateral medulla, the two principle medullary vasodepressor areas, the GiDA projects directly to the spinal cord and not to the rostral ... More
Amygdala efferents form inhibitory-type synapses with a subpopulation of catecholaminergic neurons in the rat Nucleus tractus solitarius.
AuthorsPickel VM, van Bockstaele EJ, Chan J, Cestari DM
JournalJ Comp Neurol
PubMed ID8636464
The central nucleus of the amygdala (CNA) integrates visceral responses to stress partially through efferent projections to portions of the medial nuclei of the solitary tracts (mNTS) containing catecholaminergic neurons. To determine anatomical sites for CNA modulation of these neurons, immunoperoxidase detection of anterogradely transported Phaseolus vulgaris-leucoagglutinin (PHA-L) or biotinylated ... More
Cholinergic, GABAergic, and glutamate-enriched inputs from the mesopontine tegmentum to the subthalamic nucleus in the rat.
AuthorsBevan MD, Bolam JP
JournalJ Neurosci
PubMed ID7472465
In order to clarify the origin and to examine the neurochemistry and synaptology of the projection from the mesopontine tegmentum (MTg) to the subthalamic nucleus (STN), rats received discrete deposits of anterograde tracers in different regions of the MTg. Anterogradely labeled fibers were examined in the light and electron microscopes. ... More
Characterization of a forebrain gaze field in the archistriatum of the barn owl: microstimulation and anatomical connections.
AuthorsKnudsen EI, Cohen YE, Masino T
JournalJ Neurosci
PubMed ID7623141
We present evidence that the archistriatum in the forebrain of the barn owl participates in gaze control, that it can mediate gaze changes independently of the optic tectum (OT), and that it projects in parallel to both the OT and to saccade-generating circuitry in the brainstem tegmentum. These properties are ... More
Gabapentinoid treatment promotes corticospinal plasticity and regeneration following murine spinal cord injury.
Authors
JournalJ Clin Invest
PubMed ID31793909
An injectable hydrogel enhances tissue repair after spinal cord injury by promoting extracellular matrix remodeling.
Authors
JournalNat Commun
PubMed ID28912446
Human neuroepithelial stem cell regional specificity enables spinal cord repair through a relay circuit.
AuthorsDell'Anno MT, Wang X, Onorati M, Li M, Talpo F, Sekine Y, Ma S, Liu F, Cafferty WBJ, Sestan N, Strittmatter SM
JournalNat Commun
PubMed ID30143638
'Traumatic spinal cord injury results in persistent disability due to disconnection of surviving neural elements. Neural stem cell transplantation has been proposed as a therapeutic option, but optimal cell type and mechanistic aspects remain poorly defined. Here, we describe robust engraftment into lesioned immunodeficient mice of human neuroepithelial stem cells ... More
Sub-populations of Spinal V3 Interneurons Form Focal Modules of Layered Pre-motor Microcircuits.
AuthorsChopek JW, Nascimento F, Beato M, Brownstone RM, Zhang Y
JournalCell Rep
PubMed ID30282024
'Layering of neural circuits facilitates the separation of neurons with high spatial sensitivity from those that play integrative temporal roles. Although anatomical layers are readily identifiable in the brain, layering is not structurally obvious in the spinal cord. But computational studies of motor behaviors have led to the concept of ... More
Postnatal development of retrosplenial projections to the parahippocampal region of the rat.
AuthorsSugar J, Witter MP
JournalElife
PubMed ID27008178
'The rat parahippocampal region (PHR) and retrosplenial cortex (RSC) are cortical areas important for spatial cognition. In PHR, head-direction cells are present before eye-opening, earliest detected in postnatal day (P)11 animals. Border cells have been recorded around eye-opening (P16), while grid cells do not obtain adult-like features until the fourth ... More
A Role for Auditory Corticothalamic Feedback in the Perception of Complex Sounds.
AuthorsHomma NY, Happel MFK, Nodal FR, Ohl FW, King AJ, Bajo VM
JournalJ Neurosci
PubMed ID28559384
'Feedback signals from the primary auditory cortex (A1) can shape the receptive field properties of neurons in the ventral division of the medial geniculate body (MGBv). However, the behavioral significance of corticothalamic modulation is unknown. The aim of this study was to elucidate the role of this descending pathway in ... More
Transneuronal delivery of hyper-interleukin-6 enables functional recovery after severe spinal cord injury in mice.
Authors
JournalNat Commun
PubMed ID33452250
Teneurin-3 controls topographic circuit assembly in the hippocampus.
Authors
JournalNature
PubMed ID29414938
Identification of Spinal Neurons Contributing to the Dorsal Column Projection Mediating Fine Touch and Corrective Motor Movements.
Authors
JournalNeuron
PubMed ID31586516
Restoring Cellular Energetics Promotes Axonal Regeneration and Functional Recovery after Spinal Cord Injury.
Authors
JournalCell Metab
PubMed ID32130884
2-Arachidonoylglycerol Reduces Proteoglycans and Enhances Remyelination in a Progressive Model of Demyelination.
AuthorsFeliú A, Bonilla Del Río I, Carrillo-Salinas FJ, Hernández-Torres G, Mestre L, Puente N, Ortega-Gutiérrez S, López-Rodríguez ML, Grandes P, Mecha M, Guaza C
JournalJ Neurosci
PubMed ID28751457
The failure to undergo remyelination is a critical impediment to recovery in multiple sclerosis. Chondroitin sulfate proteoglycans (CSPGs) accumulate at demyelinating lesions creating a nonpermissive environment that impairs axon regeneration and remyelination. Here, we reveal a new role for 2-arachidonoylglycerol (2-AG), the major CNS endocannabinoid, in the modulation of CSPGs ... More
Reducing Pericyte-Derived Scarring Promotes Recovery after Spinal Cord Injury.
AuthorsDias DO, Kim H, Holl D, Werne Solnestam B, Lundeberg J, Carlén M, Göritz C, Frisén J
JournalCell
PubMed ID29502968
CNS injury often severs axons. Scar tissue that forms locally at the lesion site is thought to block axonal regeneration, resulting in permanent functional deficits. We report that inhibiting the generation of progeny by a subclass of pericytes led to decreased fibrosis and extracellular matrix deposition after spinal cord injury ... More
Associative and plastic thalamic signaling to the lateral amygdala controls fear behavior.
AuthorsBarsy B, Kocsis K, Magyar A, Babiczky Á, Szabó M, Veres JM, Hillier D, Ulbert I, Yizhar O, Mátyás F
JournalNat Neurosci
PubMed ID32284608
Decades of research support the idea that associations between a conditioned stimulus (CS) and an unconditioned stimulus (US) are encoded in the lateral amygdala (LA) during fear learning. However, direct proof for the sources of CS and US information is lacking. Definitive evidence of the LA as the primary site ... More