Interaction of fluorescence labeled single-stranded DNA with hexameric DNA-helicase RepA: a photon and fluorescence correlation spectroscopy study.
Authors:Xu H, Frank J, Trier U, Hammer S, Schröder W, Behlke J, Schäfer-Korting M, Holzwarth JF, Saenger W
Journal:Biochemistry
PubMed ID:11401568
'Fluorescence correlation spectroscopy (FCS) was used to characterize the interaction of fluorescence labeled single-stranded DNA (ssDNA) with hexameric RepA DNA-helicase (hRepA) encoded by plasmid RSF1010. The apparent dissociation constants, Kd(app), for the equilibrium binding of 12mer, 30mer, and 45mer ssDNA 5''-labeled with BFL to hRepA dimer in the presence of ... More