Wavelength-resolved fluorescence detection in capillary electrophoresis.
AuthorsTimperman AT, Khatib K, Sweedler JV
JournalAnal Chem
PubMed ID7864386
'A multichannel laser-induced fluorescence detector for capillary electrophoresis is described. The detection system combines yoctomole limits of detection with the simultaneous acquisition of entire fluorescence emission spectra. An Ar/Kr mixed-gas ion laser provides great flexibility in excitation wavelengths, and a holographic grating and charge-coupled device detector combination allows a 500-nm ... More
FRET analysis of protein conformational change through position-specific incorporation of fluorescent amino acids.
AuthorsKajihara D, Abe R, Iijima I, Komiyama C, Sisido M, Hohsaka T
JournalNat Methods
PubMed ID17060916
'We designed and synthesized new, fluorescent, non-natural amino acids that emit fluorescence of wavelengths longer than 500 nm and are accepted by an Escherichia coli cell-free translation system. We synthesized p-aminophenylalanine derivatives linked with BODIPY fluorophores at the p-amino group and introduced them into streptavidin using the four-base codon CGGG ... More
Detection of the DeltaF508 mutation in the CFTR gene by means of time-resolved fluorescence methods.
AuthorsKirschstein S, Winter S, Turner D, Löber G
JournalBioelectrochem Bioenerg
PubMed ID10379562
A rapid recognition in the base sequence of nucleic acids is an important prerequisite toward the diagnosis of genetic diseases and their carrier states. We have developed a hybridisation method in which a fluorescently labeled oligonucleotide is used to detect point mutations in a target by a simple fluorescence lifetime ... More
Molecular dynamics of labeled oligonucleotide probes used for the detection of point mutations in DNA.
AuthorsWinter S, Kirschstein S, Miller DP
JournalNucleosides Nucleotides
PubMed ID10408923
The fluorescent label BODIPY 576-589 linked to the 5-end of an oligonucleotide via alkyl chain linkers can be used as a probe to detect point mutations in DNA. We have employed fluorescence anisotropy decay and dynamic fluorescence resonance energy transfer (FRET) in order to investigate the molecular origin of the ... More
Systematic evaluation of transcellular activities of secretory phospholipases A2. High activity of group V phospholipases A2 to induce eicosanoid biosynthesis in neighboring inflammatory cells.
AuthorsWijewickrama GT, Kim JH, Kim YJ, Abraham A, Oh Y, Ananthanarayanan B, Kwatia M, Ackerman SJ, Cho W
JournalJ Biol Chem
PubMed ID16476735
The mechanisms by which secretory phospholipase A2 (PLA2) exerts cellular effects are not fully understood. To elucidate these mechanisms, we systematically and quantitatively assessed the activities of human group IIA, V, and X PLA2s on originating and neighboring cells using orthogonal fluorogenic substrates in various mixed cell systems. When HEK293 ... More
Receptor-induced internalization of selective peptidic mu and delta opioid ligands.
AuthorsGaudriault G, Nouel D, Dal Farra C, Beaudet A, Vincent JP
JournalJ Biol Chem
PubMed ID9006932
The binding and internalization of radioiodinated and fluorescent mu and delta opioid peptides in mammalian cells were quantitatively studied by biochemical techniques and directly visualized by confocal microscopy. The labeled peptides were prepared by inserting either a 125I-Bolton-Hunter group or a fluorescent probe into the C-terminal part of 5-aminopentylamide derivatives ... More
Direct, ultrasensitive, and selective optical detection of protein toxins using multivalent interactions.
AuthorsSong X, Swanson BI
JournalAnal Chem
PubMed ID10366891
Three highly sensitive, selective, and reagent-free optical signal transduction methods for detection of polyvalent proteins have been developed by directly coupling distance-dependent fluorescence self-quenching and/or resonant-energy transfer to the protein-receptor binding events. The ganglioside GM1, as the recognition unit for cholera toxin (CT), was covalently labeled with fluorophores and then ... More
Differential internalization of somatostatin in COS-7 cells transfected with SST1 and SST2 receptor subtypes: a confocal microscopic study using novel fluorescent somatostatin derivatives.
AuthorsNouel D, Gaudriault G, Houle M, Reisine T, Vincent JP, Mazella J, Beaudet A
JournalEndocrinology
PubMed ID8977417
A growing body of evidence suggests that neuropeptide binding to G protein-linked receptors may result in internalization of receptor-ligand complexes, followed by intracellular mobilization and degradation of the ligand into its target cells. Because of discrepant results in the literature concerning the occurrence of such a mechanism for the tetradecapeptide ... More
Morphine-induced changes in delta opioid receptor trafficking are linked to somatosensory processing in the rat spinal cord.
AuthorsMorinville A, Cahill CM, Aibak H, Rymar VV, Pradhan A, Hoffert C, Mennicken F, Stroh T, Sadikot AF, O'Donnell D, Clarke PB, Collier B, Henry JL, Vincent JP, Beaudet A
JournalJ Neurosci
PubMed ID15201327
An in vivo fluorescent deltorphin (Fluo-DLT) internalization assay was used to assess the distribution and regulation of pharmacologically available delta opioid receptors (deltaORs) in the rat lumbar (L4-5) spinal cord. Under basal conditions, intrathecal injection of Fluo-DLT resulted in the labeling of numerous deltaOR-internalizing neurons throughout dorsal and ventral horns. ... More