The membrane-bound histidine acid phosphatase TbMBAP1 is essential for endocytosis and membrane recycling in Trypanosoma brucei.
AuthorsEngstler M, Weise F, Bopp K, Grünfelder CG, Günzel M, Heddergott N, Overath P
JournalJ Cell Sci
PubMed ID15855239
'In the parasitic protozoan Trypanosoma brucei, endocytosis and exocytosis occur exclusively at an invagination of the plasma membrane around the base of the flagellum, called the flagellar pocket, which actively communicates by vesicular membrane flow with cisternal/tubulovesicular endosomes. The division of the cell surface into three morphologically distinct sub-domains and ... More
Viral nanoparticles as tools for intravital vascular imaging.
AuthorsLewis JD, Destito G, Zijlstra A, Gonzalez MJ, Quigley JP, Manchester M, Stuhlmann H
JournalNat Med
PubMed ID16501571
'A significant impediment to the widespread use of noninvasive in vivo vascular imaging techniques is the current lack of suitable intravital imaging probes. We describe here a new strategy to use viral nanoparticles as a platform for the multivalent display of fluorescent dyes to image tissues deep inside living organisms. ... More
Human ESCRT-II complex and its role in human immunodeficiency virus type 1 release.
AuthorsLangelier C, von Schwedler UK, Fisher RD, De Domenico I, White PL, Hill CP, Kaplan J, Ward D, Sundquist WI
JournalJ Virol
PubMed ID16973552
'The budding of many enveloped RNA viruses, including human immunodeficiency virus type 1 (HIV-1), requires some of the same cellular machinery as vesicle formation at the multivesicular body (MVB). In Saccharomyces cerevisiae, the ESCRT-II complex performs a central role in MVB protein sorting and vesicle formation, as it is recruited ... More
On the use of retrograde tracers for identification of axon collaterals with multiple fluorescent retrograde tracers.
AuthorsSchofield BR, Schofield RM, Sorensen KA, Motts SD
JournalNeuroscience
PubMed ID17379419
'A common method for identifying collateral projections is to inject different retrograde tracers into two targets and examine labeled cells for the presence of both tracers. Double-labeled cells are considered to have collateral projections to the two injection sites. This method is widely considered to underestimate the extent of collaterals. ... More
Visually guided injection of identified reticulospinal neurons in zebrafish: a survey of spinal arborization patterns.
AuthorsGahtan E, O'Malley DM
JournalJ Comp Neurol
PubMed ID12640669
'We report here the pattern of axonal branching for 11 descending cell types in the larval brainstem; eight of these cell types are individually identified neurons. Large numbers of brainstem neurons were retrogradely labeled in living larvae by injecting Texas-red dextran into caudal spinal cord. Subsequently, in each larva a ... More
Activation of trypsinogen in large endocytic vacuoles of pancreatic acinar cells.
'The intracellular activation of trypsinogen, which is both pH- and calcium-dependent, is an important early step in the development of acute pancreatitis. The cellular compartment in which trypsinogen activation occurs currently is unknown. We therefore investigated the site of intracellular trypsinogen activation by using an established cellular model of acute ... More
Autophagy is disrupted in a knock-in mouse model of juvenile neuronal ceroid lipofuscinosis.
AuthorsCao Y, Espinola JA, Fossale E, Massey AC, Cuervo AM, MacDonald ME, Cotman SL
JournalJ Biol Chem
PubMed ID16714284
'Juvenile neuronal ceroid lipofuscinosis is caused by mutation of a novel, endosomal/lysosomal membrane protein encoded by CLN3. The observation that the mitochondrial ATPase subunit c protein accumulates in this disease suggests that autophagy, a pathway that regulates mitochondrial turnover, may be disrupted. To test this hypothesis, we examined the autophagic ... More
Apoptosis-inducing factor is involved in the regulation of caspase-independent neuronal cell death.
'Caspase-independent death mechanisms have been shown to execute apoptosis in many types of neuronal injury. P53 has been identified as a key regulator of neuronal cell death after acute injury such as DNA damage, ischemia, and excitotoxicity. Here, we demonstrate that p53 can induce neuronal cell death via a caspase-mediated ... More
Adenovirus-facilitated nuclear translocation of adeno-associated virus type 2.
AuthorsXiao W, Warrington KH, Hearing P, Hughes J, Muzyczka N
JournalJ Virol
PubMed ID12388712
'We examined cytoplasmic trafficking and nuclear translocation of adeno-associated virus type 2 (AAV) by using Alexa Fluor 488-conjugated wild-type AAV, A20 monoclonal antibody immunocytochemistry, and subcellular fractionation techniques followed by DNA hybridization. Our results indicated that in the absence of adenovirus (Ad), AAV enters the cell rapidly and escapes from ... More
Homotypic fusion of early endosomes: SNAREs do not determine fusion specificity.
AuthorsBrandhorst D, Zwilling D, Rizzoli SO, Lippert U, Lang T, Jahn R
JournalProc Natl Acad Sci U S A
PubMed ID16469845
'Membrane fusion in the secretory pathway is mediated by soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) proteins. Different fusion steps are thought to be effected by independent sets of SNAREs, but it is unclear whether specificity is determined by an intrinsic specificity of SNARE pairing or by upstream factors. Using a ... More
Adenocarcinoma of the pancreas in childhood. Report of a case and a review of the English language literature.
AuthorsTaxy JB
JournalCancer
PubMed ID1260669
'A case of adenocarcinoma of the head of the pancreas in a 13-year-old girl is reported. Some areas simulated an islet cell tumor by light microscopy, but contained numerous eosinophilic granules which were PAS-positive and diastase resistant. Ultrastructurally, the granules were large (960 mum-3000 mum in diameter) and electron-dense, resembling ... More
Visualizing formation and dynamics of vacuoles in living cells using contrasting dextran-bound indicator: endocytic and nonendocytic vacuoles.
'Here we describe a technique that allows us to visualize in real time the formation and dynamics (fusion, changes of shape, and translocation) of vacuoles in living cells. The technique involves infusion of a dextran-bound fluorescent probe into the cytosol of the cell via a patch pipette, using the whole-cell ... More
Evidence for early endosome-like fusion of recently endocytosed synaptic vesicles.
AuthorsRizzoli SO, Bethani I, Zwilling D, Wenzel D, Siddiqui TJ, Brandhorst D, Jahn R
JournalTraffic
PubMed ID17004320
'Early endosomes are well-established acceptor compartments of endocytic vesicles in many cell types. Little evidence of their existence or function has been obtained in synapses, and it is generally believed that synaptic vesicles recycle without passing through an endosomal intermediate. We show here that the early endosomal SNARE proteins are ... More
Monitoring the permeability of the nuclear envelope during the cell cycle.
AuthorsLénárt P, Ellenberg J,
JournalMethods
PubMed ID16343937
'In animal organisms the nuclear envelope (NE) dis-assembles during cell division resulting in complete intermixing of cytoplasmic and nuclear compartments. This leads to the activation of many mitotic enzymes, which were kept away from their substrates or regulators by nuclear or cytoplasmic sequestration in interphase. Nuclear envelope breakdown (NEBD) is ... More
CE analysis of the acidic organelles of a single cell.
AuthorsChen Y, Xiong G, Arriaga EA
JournalElectrophoresis
PubMed ID17577888
'The properties of organelles within a cell have been shown to be highly heterogeneous. Until now, it has been unclear just how much of this heterogeneity is endemic to the organelle subpopulations themselves and how much is actually due to stochastic cellular noise. An attractive approach for investigating the origins ... More
Lipoprotein aggregation protects human monocyte-derived macrophages from OxLDL-induced cytotoxicity.
AuthorsAsmis R, Begley JG, Jelk J, Everson WV
JournalJ Lipid Res
PubMed ID15772426
'Oxidative modifications render low density lipoprotein cytotoxic and enhance its propensity to aggregate and fuse into particles similar to those found in atherosclerotic lesions. We showed previously that aggregation of oxidized LDL (OxLDL) promotes the transformation of human macrophages into lipid-laden foam cells (Asmis, R., and J. Jelk. 2000. Large ... More
Evolutionary origins for social vocalization in a vertebrate hindbrain-spinal compartment.
AuthorsBass AH, Gilland EH, Baker R,
JournalScience
PubMed ID18635807
'The macroevolutionary events leading to neural innovations for social communication, such as vocalization, are essentially unexplored. Many fish vocalize during female courtship and territorial defense, as do amphibians, birds, and mammals. Here, we map the neural circuitry for vocalization in larval fish and show that the vocal network develops in ... More
Dynamic microcompartmentation in synthetic cells.
AuthorsLong MS, Jones CD, Helfrich MR, Mangeney-Slavin LK, Keating CD
JournalProc Natl Acad Sci U S A
PubMed ID15788532
'An experimental model for cytoplasmic organization is presented. We demonstrate dynamic control over protein distribution within synthetic cells comprising a lipid bilayer membrane surrounding an aqueous polymer solution. This polymer solution generally exists as two immiscible aqueous phases. Protein partitioning between these phases leads to microcompartmentation, or heterogeneous protein distribution ... More
Motorized RhoGAP myosin IXb (Myo9b) controls cell shape and motility.
AuthorsHanley PJ, Xu Y, Kronlage M, Grobe K, Schön P, Song J, Sorokin L, Schwab A, Bähler M,
JournalProc Natl Acad Sci U S A
PubMed ID20566876
Directional motility is a fundamental function of immune cells, which are recruited to sites of pathogen invasion or tissue damage by chemoattractant signals. To move, cells need to generate lamellipodial membrane protrusions at the front and retract the trailing end. These elementary events are initiated by Rho-family GTPases, which cycle ... More
Fusion pore dynamics and insulin granule exocytosis in the pancreatic islet.
AuthorsTakahashi N, Kishimoto T, Nemoto T, Kadowaki T, Kasai H
JournalScience
PubMed ID12193788
Insulin secretion from intact mouse pancreatic islets was investigated with two-photon excitation imaging. Insulin granule exocytosis occurred mainly toward the interstitial space, away from blood vessels. The fusion pore was unusually stable with a lifetime of 1.8 seconds. Opening of the 1.4-nanometer-diameter pore was preceded by unrestricted lateral diffusion of ... More
Optical imaging of neuronal populations during decision-making.
AuthorsBriggman KL, Abarbanel HD, Kristan WB
JournalScience
PubMed ID15705844
We investigated decision-making in the leech nervous system by stimulating identical sensory inputs that sometimes elicit crawling and other times swimming. Neuronal populations were monitored with voltage-sensitive dyes after each stimulus. By quantifying the discrimination time of each neuron, we found single neurons that discriminate before the two behaviors are ... More
Location of bladder and urethral sphincter motoneurons in the male guinea pig (Cavia porcellus).
AuthorsKuipers R, Izhar Z, Gerrits PO, Miner W, Holstege G
JournalNeurosci Lett
PubMed ID15147780
Although the guinea pig is used widely in experimental medical research, including in studies on micturition control, the spinal origin of preganglionic parasympathetic bladder and somatic external urethral sphincter motoneurons is not known. In the male guinea pig using wheat germ agglutinin-conjugated horseradish peroxidase and dextran Alexa Fluor 488/568 tracers, ... More
Monitoring autophagy in lysosomal storage disorders.
AuthorsRaben N, Shea L, Hill V, Plotz P,
JournalMethods Enzymol
PubMed ID19216919
Lysosomes are the final destination of the autophagic pathway. It is in the acidic milieu of the lysosomes that autophagic cargo is metabolized and recycled. One would expect that diseases with primary lysosomal defects would be among the first systems in which autophagy would be studied. In reality, this is ... More
Modern mosaic analysis in the zebrafish.
AuthorsCarmany-Rampey A, Moens CB,
JournalMethods
PubMed ID16829130
One of the most powerful tools used to gain insight into complex developmental processes is the analysis of mosaic embryos. A mosaic is defined as an organism that contains cells of more than one genotype, usually wild-type and mutant. It is the interplay between wild-type and mutant cells in the ... More
Glycolipid trafficking in Drosophila undergoes pathway switching in response to aberrant cholesterol levels.
AuthorsHortsch R, Lee E, Erathodiyil N, Hebbar S, Steinert S, Lee JY, Chua DS, Kraut R,
JournalMol Biol Cell
PubMed ID20053687
In lipid storage diseases, the intracellular trafficking of sphingolipids is altered by conditions of aberrant cholesterol accumulation. Drosophila has been used recently to model lipid storage diseases, but the effects of sterol accumulation on sphingolipid trafficking are not known in the fly, and the trafficking of sphingolipids in general has ... More
Pincher, a pinocytic chaperone for nerve growth factor/TrkA signaling endosomes.
AuthorsShao Y, Akmentin W, Toledo-Aral JJ, Rosenbaum J, Valdez G, Cabot JB, Hilbush BS, Halegoua S
JournalJ Cell Biol
PubMed ID12011113
A central tenet of nerve growth factor (NGF) action that is poorly understood is its ability to mediate cytoplasmic signaling, through its receptor TrkA, that is initiated at the nerve terminal and conveyed to the soma. We identified an NGF-induced protein that we termed Pincher (pinocytic chaperone) that mediates endocytosis ... More
TrkB has a cell-autonomous role in the establishment of hippocampal Schaffer collateral synapses.
AuthorsLuikart BW, Nef S, Virmani T, Lush ME, Liu Y, Kavalali ET, Parada LF
JournalJ Neurosci
PubMed ID15829629
Neurotrophin signaling has been implicated in the processes of synapse formation and plasticity. To gain additional insight into the mechanism of BDNF and TrkB influence on synapse formation and synaptic plasticity, we generated a conditional knock-out for TrkB using the cre/loxp system. Using three different cre-expressing transgenic mice, three unique ... More
TbVps34, the trypanosome orthologue of Vps34, is required for Golgi complex segregation.
AuthorsHall BS, Gabernet-Castello C, Voak A, Goulding D, Natesan SK, Field MC
JournalJ Biol Chem
PubMed ID16835237
Phosphoinositides are important regulators of numerous cellular functions. The yeast class III phosphatidylinositol 3-kinase Vps34p, and its human orthologue hVPS34, are implicated in control of several key pathways, including endosome to lysosome transport, retrograde endosome to Golgi traffic, multivesicular body formation, and autophagy. We have identified the Vps34p orthologue in ... More
Projections from primary somatosensory cortex to the neostriatum: the role of somatotopic continuity in corticostriatal convergence.
AuthorsHoover JE, Hoffer ZS, Alloway KD
JournalJ Neurophysiol
PubMed ID12611938
We characterized the organization of corticostriatal projections from rodent primary somatosensory cortex (SI), testing the hypothesis that projections from SI areas representing subcomponents of the forelimb exhibit greater neostriatal overlap than projections from areas representing separate body parts. The anterograde tracers Fluoro-Ruby (FR), Alexa Fluor (AF), and biotinylated dextran amine ... More
Differential type I interferon activation and susceptibility of dendritic cell populations to porcine arterivirus.
AuthorsLoving CL, Brockmeier SL, Sacco RE
JournalImmunology
PubMed ID17116172
Dendritic cells (DCs) play a role in anti-viral immunity by providing early innate protection against viral replication and by presenting antigen to T cells for initiation of the adaptive immune response. Studies show the adaptive response to porcine reproductive and respiratory syndrome virus (PRRSV) is ineffective for complete viral elimination. ... More
A single genetic locus controls both expression of DPEP1/CHMP1A and kidney disease development via ferroptosis.
Authors
JournalNat Commun
PubMed ID34426578
The Smurf ubiquitin ligases regulate tissue separation via antagonistic interactions with ephrinB1.
Authors
JournalGenes Dev
PubMed ID23475958
Vascular and blood-brain barrier-related changes underlie stress responses and resilience in female mice and depression in human tissue.
Authors
JournalNat Commun
PubMed ID35013188
Spatially and temporally defined lysosomal leakage facilitates mitotic chromosome segregation.
Authors
JournalNat Commun
PubMed ID31932607
Reduced thermal tolerance in a coral carrying CRISPR-induced mutations in the gene for a heat-shock transcription factor.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID33168726
Discrete Evaluative and Premotor Circuits Enable Vocal Learning in Songbirds.
Authors
JournalNeuron
PubMed ID31447169
Microinjection of mRNA or morpholinos for reverse genetic analysis in the starlet sea anemone, Nematostella vectensis.
Authors
JournalNat Protoc
PubMed ID23579781
A Basal Ganglia Circuit Sufficient to Guide Birdsong Learning.
Authors
JournalNeuron
PubMed ID29551492
Fate restriction and multipotency in retinal stem cells.
Authors
JournalCell Stem Cell
PubMed ID22136930
Pericyte-to-endothelial cell signaling via vitronectin-integrin regulates blood-CNS barrier.
Authors
JournalNeuron
PubMed ID35294899
Spinal Interneurons With Dual Axon Projections to Knee-Extensor and Hip-Extensor Motor Pools.
AuthorsNguyen KH, Scheurich TE, Gu T, Berkowitz A
JournalFront Neural Circuits
PubMed ID32226362
The central nervous system (CNS) may simplify control of limb movements by activating certain combinations of muscles together, i.e., muscle synergies. Little is known, however, about the spinal cord interneurons that activate muscle synergies by exciting sets of motoneurons for different muscles. The turtle spinal cord, even without brain inputs ... More
Reverse Genetic Approaches to Investigate the Neurobiology of the Cnidarian Sea Anemone Nematostella vectensis.
AuthorsHavrilak JA, Layden MJ
JournalMethods Mol Biol
PubMed ID31552647
The cnidarian sea anemone Nematostella vectensis has grown in popularity as a model system to complement the ongoing work in traditional bilaterian model species (e.g. Drosophila, C. elegans, vertebrate). The driving force behind developing cnidarian model systems is the potential of this group of animals to impact EvoDevo studies aimed ... More
In vitro investigation of hyaluronan-based polymeric micelles for drug delivery into the skin: The internalization pathway.
AuthorsStarigazdová J, Nešporová K, Cepa M, Šínová R, Šmejkalová D, Huerta-Angeles G, Velebný V
JournalEur J Pharm Sci
PubMed ID31783157
In our previous research, we concluded that polymeric micelles based on hyaluronic acid are able to penetrate into the deeper layers of skin tissue. The aim of this work was to characterize the mechanisms involved in the uptake by skin cells, which is important for understanding the influence of the ... More