Visually guided injection of identified reticulospinal neurons in zebrafish: a survey of spinal arborization patterns.
AuthorsGahtan E, O'Malley DM
JournalJ Comp Neurol
PubMed ID12640669
'We report here the pattern of axonal branching for 11 descending cell types in the larval brainstem; eight of these cell types are individually identified neurons. Large numbers of brainstem neurons were retrogradely labeled in living larvae by injecting Texas-red dextran into caudal spinal cord. Subsequently, in each larva a ... More
Beclin1-binding UVRAG targets the class C Vps complex to coordinate autophagosome maturation and endocytic trafficking.
AuthorsLiang C, Lee JS, Inn KS, Gack MU, Li Q, Roberts EA, Vergne I, Deretic V, Feng P, Akazawa C, Jung JU,
JournalNat Cell Biol
PubMed ID18552835
'Autophagic and endocytic pathways are tightly regulated membrane rearrangement processes that are crucial for homeostasis, development and disease. Autophagic cargo is delivered from autophagosomes to lysosomes for degradation through a complex process that topologically resembles endosomal maturation. Here, we report that a Beclin1-binding autophagic tumour suppressor, UVRAG, interacts with the ... More
COPII-Golgi protein interactions regulate COPII coat assembly and Golgi size.
AuthorsGuo Y, Linstedt AD
JournalJ Cell Biol
PubMed ID16818719
'Under experimental conditions, the Golgi apparatus can undergo de novo biogenesis from the endoplasmic reticulum (ER), involving a rapid phase of growth followed by a return to steady state, but the mechanisms that control growth are unknown. Quantification of coat protein complex (COP) II assembly revealed a dramatic up-regulation at ... More
Correlative light-electron microscopy (CLEM) combining live-cell imaging and immunolabeling of ultrathin cryosections.
'The visualization of fluorescent proteins in living cells is a powerful approach to study intracellular dynamics. A limitation of fluorescence imaging, however, is that it lacks fine structural information; a fluorescent spot could represent an entire organelle, an organellar subdomain or even aggregates of proteins or membranes. These limitations can ... More
Automated organelle-based colocalization in whole-cell imaging.
AuthorsWoodcroft BJ, Hammond L, Stow JL, Hamilton NA,
JournalCytometry A
PubMed ID19746416
The use of fluorescence microscopy to investigate protein colocalization is an invaluable tool for understanding subcellular structures and their associated proteins. However, current techniques are largely limited to two-dimensional (2D) imaging and often require manual segmentation. Here, we present OBCOL, a methodology to automatically segment and quantify protein colocalization not ... More
Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis.
AuthorsChipuk JE, Kuwana T, Bouchier-Hayes L, Droin NM, Newmeyer DD, Schuler M, Green DR
JournalScience
PubMed ID14963330
The tumor suppressor p53 exerts its anti-neoplastic activity primarily through the induction of apoptosis. We found that cytosolic localization of endogenous wild-type or trans-activation-deficient p53 was necessary and sufficient for apoptosis. p53 directly activated the proapoptotic Bcl-2 protein Bax in the absence of other proteins to permeabilize mitochondria and engage ... More
Gbetagamma acts at the C terminus of SNAP-25 to mediate presynaptic inhibition.
Presynaptic inhibition mediated by G protein-coupled receptors may involve a direct interaction between G proteins and the vesicle fusion machinery. The molecular target of this pathway is unknown. We demonstrate that Gbetagamma-mediated presynaptic inhibition in lamprey central synapses occurs downstream from voltage-gated Ca(2+) channels. Using presynaptic microinjections of botulinum toxins ... More
A UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase is required for epithelial tube formation.
AuthorsTian E, Ten Hagen KG
JournalJ Biol Chem
PubMed ID17098739
Epithelial tubes are essential for the proper function of a diverse array of eukaryotic organs. Here we present a novel class of genes required for maintaining epithelial cell shape, polarity, and paracellular barrier function in the Drosophila embryonic tracheal system. Mutations in one member of the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase family (pgant35A) ... More
Cell ratcheting through the Sbf RabGEF directs force balancing and stepped apical constriction.
Authors
JournalJ Cell Biol
PubMed ID31562231
Vocal experimentation in the juvenile songbird requires a basal ganglia circuit.
Authors
JournalPLoS Biol
PubMed ID15826219
Proliferation-independent regulation of organ size by Fgf/Notch signaling.
Authors
JournalElife
PubMed ID28085667
Hemostasis stimulates lymphangiogenesis through release and activation of VEGFC.
Authors
JournalBlood
PubMed ID31562136
By Capturing Inflammatory Lipids Released from Dying Cells, the Receptor CD14 Induces Inflammasome-Dependent Phagocyte Hyperactivation.
Authors
JournalImmunity
PubMed ID29045901
Neto-α Controls Synapse Organization and Homeostasis at the Drosophila Neuromuscular Junction.
Authors
JournalCell Rep
PubMed ID32640231
Lysosome-Rich Enterocytes Mediate Protein Absorption in the Vertebrate Gut.
Authors
JournalDev Cell
PubMed ID31474562
Lung emphysema and impaired macrophage elastase clearance in mucolipin 3 deficient mice.
Authors
JournalNat Commun
PubMed ID35031603
Histone demethylase Lsd1 is required for the differentiation of neural cells in Nematostella vectensis.
Authors
JournalNat Commun
PubMed ID35075108
Enhanced enzymatic production of cholesteryl 6'-acylglucoside impairs lysosomal degradation for the intracellular survival of Helicobacter pylori.