Glycosylation influences the lectin activities of the macrophage mannose receptor.
AuthorsSu Y, Bakker T, Harris J, Tsang C, Brown GD, Wormald MR, Gordon S, Dwek RA, Rudd PM, Martinez-Pomares L
JournalJ Biol Chem
PubMed ID15983039
'The mannose receptor (MR) is a heavily glycosylated endocytic receptor that recognizes both mannosylated and sulfated ligands through its C-type lectin domains and cysteine-rich (CR) domain, respectively. Differential binding properties have been described for MR isolated from different sources, and we hypothesized that this could be due to altered glycosylation. ... More
Receptor (CD155)-dependent endocytosis of poliovirus and retrograde axonal transport of the endosome.
AuthorsOhka S, Matsuda N, Tohyama K, Oda T, Morikawa M, Kuge S, Nomoto A
JournalJ Virol
PubMed ID15194795
'Poliovirus (PV), when injected intramuscularly into the calf, is incorporated into the sciatic nerve and causes an initial paralysis of the inoculated limb in transgenic mice carrying the human PV receptor (hPVR/CD155) gene. Here, we demonstrated by using an immunoelectron microscope that PV particles exist on vesicle structures in nerve ... More
Characterizing peptide-mediated DNA internalization in human cancer cells.
AuthorsWittrup A, Belting M,
JournalMethods Mol Biol
PubMed ID19085116
'Cell penetrating peptides (CPPs) are currently used to deliver various macromolecular cargos to intracellular sites of action both in vitro and in vivo on an experimental basis. During the last few years, even more evidence has accumulated indicating that the main route of entry for most CPPs is through endocytosis ... More
Control of cellular physiology by TM9 proteins in yeast and Dictyostelium.
AuthorsFroquet R, Cherix N, Birke R, Benghezal M, Cameroni E, Letourneur F, Mösch HU, De Virgilio C, Cosson P,
JournalJ Biol Chem
PubMed ID18178563
'TM9 proteins constitute a well defined family, characterized by the presence of a large variable extracellular domain and nine putative transmembrane domains. This family is highly conserved throughout evolution and comprises three members in Dictyostelium discoideum and Saccharomyces cerevisiae and four in humans and mice. In Dictyostelium, previous analysis demonstrated ... More
A stepwise dissection of the intracellular fate of cationic cell-penetrating peptides.
AuthorsFischer R, Köhler K, Fotin-Mleczek M, Brock R
JournalJ Biol Chem
PubMed ID14707144
'The role of endosomal acidification and retrograde transport for the uptake of the highly basic cell-penetrating peptides penetratin, Tat, and oligoarginine was investigated. The effect of a panel of drugs that interfere with discrete steps of endocytosis or Golgi-mediated transport on uptake and cellular distribution of fluorescein-labeled peptide analogues was ... More
Silica crystals and aluminum salts activate the NALP3 inflammasome through phagosomal destabilization.
AuthorsHornung V, Bauernfeind F, Halle A, Samstad EO, Kono H, Rock KL, Fitzgerald KA, Latz E,
JournalNat Immunol
PubMed ID18604214
'Inhalation of silica crystals causes inflammation in the alveolar space. Prolonged exposure to silica can lead to the development of silicosis, an irreversible, fibrotic pulmonary disease. The mechanisms by which silica and other crystals activate immune cells are not well understood. Here we demonstrate that silica and aluminum salt crystals ... More
Visualizing the site and dynamics of IgG salvage by the MHC class I-related receptor, FcRn.
AuthorsOber RJ, Martinez C, Vaccaro C, Zhou J, Ward ES
JournalJ Immunol
PubMed ID14764666
'The MHC class I-related receptor, FcRn, plays a central role in regulating the serum levels of IgG. FcRn is expressed in endothelial cells, suggesting that these cells may be involved in maintaining IgG levels. We have used live cell imaging of FcRn-green fluorescent protein transfected human endothelial cells to analyze ... More
The fate of Pluronic F-68 in chondrocytes and CHO cells.
AuthorsGigout A, Buschmann MD, Jolicoeur M,
JournalBiotechnol Bioeng
PubMed ID18393312
'The surfactant Pluronic F-68 (PF-68) is widely used in large-scale mammalian cell culture to protect cells from shear stress that arises from agitation and gas sparging. Several studies suggested that PF-68 is incorporated into the cell plasma membrane and could enter the cells, but without providing any direct evidence. The ... More
Cyclic AMP-induced repair of zebrafish spinal circuits.
AuthorsBhatt DH, Otto SJ, Depoister B, Fetcho JR
JournalScience
PubMed ID15247482
Neurons in the human central nervous system (CNS) are unable to regenerate, as a result of both an inhibitory environment and their inherent inability to regrow. In contrast, the CNS environment in fish is permissive for growth, yet some neurons still cannot regenerate. Fish thus offer an opportunity to study ... More
Engineering the Fc region of immunoglobulin G to modulate in vivo antibody levels.
AuthorsVaccaro C, Zhou J, Ober RJ, Ward ES
JournalNat Biotechnol
PubMed ID16186811
We have engineered the Fc region of a human immunoglobulin G (IgG) to generate a mutated antibody that modulates the concentrations of endogenous IgGs in vivo. This has been achieved by targeting the activity of the Fc receptor, FcRn, which serves through its IgG salvage function to maintain and regulate ... More
Neuronal birth order identifies a dimorphic sensorineural map.
AuthorsPujol-Martí J, Zecca A, Baudoin JP, Faucherre A, Asakawa K, Kawakami K, López-Schier H,
JournalJ Neurosci
PubMed ID22378871
Spatially distributed sensory information is topographically mapped in the brain by point-to-point correspondence of connections between peripheral receptors and central target neurons. In fishes, for example, the axonal projections from the mechanosensory lateral line organize a somatotopic neural map. The lateral line provides hydrodynamic information for intricate behaviors such as ... More
Test samples for optimizing STORM super-resolution microscopy.
AuthorsMetcalf DJ, Edwards R, Kumarswami N, Knight AE,
Journal
PubMed ID24056752
STORM is a recently developed super-resolution microscopy technique with up to 10 times better resolution than standard fluorescence microscopy techniques. However, as the image is acquired in a very different way than normal, by building up an image molecule-by-molecule, there are some significant challenges for users in trying to optimize ... More
Correlative microscopy of densely labeled projection neurons using neural tracers.
AuthorsOberti D, Kirschmann MA, Hahnloser RH,
JournalFront Neuroanat
PubMed ID20676237
Three-dimensional morphological information about neural microcircuits is of high interest in neuroscience, but acquiring this information remains challenging. A promising new correlative technique for brain imaging is array tomography (Micheva and Smith, 2007), in which series of ultrathin brain sections are treated with fluorescent antibodies against neurotransmitters and synaptic proteins. ... More
Glycolipid trafficking in Drosophila undergoes pathway switching in response to aberrant cholesterol levels.
AuthorsHortsch R, Lee E, Erathodiyil N, Hebbar S, Steinert S, Lee JY, Chua DS, Kraut R,
JournalMol Biol Cell
PubMed ID20053687
In lipid storage diseases, the intracellular trafficking of sphingolipids is altered by conditions of aberrant cholesterol accumulation. Drosophila has been used recently to model lipid storage diseases, but the effects of sterol accumulation on sphingolipid trafficking are not known in the fly, and the trafficking of sphingolipids in general has ... More
Mammalian reovirus, a nonfusogenic nonenveloped virus, forms size-selective pores in a model membrane.
AuthorsAgosto MA, Ivanovic T, Nibert ML
JournalProc Natl Acad Sci U S A
PubMed ID17053074
During cell entry, reovirus particles with a diameter of 70-80 nm must penetrate the cellular membrane to access the cytoplasm. The mechanism of penetration, without benefit of membrane fusion, is not well characterized for any such nonenveloped animal virus. Lysis of RBCs is an in vitro assay for the membrane ... More
Nuclear import time and transport efficiency depend on importin beta concentration.
AuthorsYang W, Musser SM
JournalJ Cell Biol
PubMed ID16982803
Although many components and reaction steps necessary for bidirectional transport across the nuclear envelope (NE) have been characterized, the mechanism and control of cargo migration through nuclear pore complexes (NPCs) remain poorly understood. Single-molecule fluorescence microscopy was used to track the movement of cargos before, during, and after their interactions ... More
Control of local actin assembly by membrane fusion-dependent compartment mixing.
AuthorsYu HY, Bement WM
JournalNat Cell Biol
PubMed ID17237773
Local actin assembly is associated with sites of exocytosis in processes ranging from phagocytosis to compensatory endocytosis. Here, we examine whether the trigger for actin-coat assembly around exocytosing Xenopus egg cortical granules is 'compartment mixing'--the union of the contents of the plasma membrane with that of the secretory granule membrane. ... More
Vectorial insertion of apical and basolateral membrane proteins in polarized epithelial cells revealed by quantitative 3D live cell imaging.
AuthorsHua W, Sheff D, Toomre D, Mellman I
JournalJ Cell Biol
PubMed ID16567501
Although epithelial cells are known to exhibit a polarized distribution of membrane components, the pathways responsible for delivering membrane proteins to their appropriate domains remain unclear. Using an optimized approach to three-dimensional live cell imaging, we have visualized the transport of newly synthesized apical and basolateral membrane proteins in fully ... More
Myosin VI regulates endocytosis of the cystic fibrosis transmembrane conductance regulator.
AuthorsSwiatecka-Urban A, Boyd C, Coutermarsh B, Karlson KH, Barnaby R, Aschenbrenner L, Langford GM, Hasson T, Stanton BA
JournalJ Biol Chem
PubMed ID15247260
The cystic fibrosis transmembrane conductance regulator (CFTR) is a cyclic AMP-regulated Cl(-) channel expressed in the apical plasma membrane in fluid-transporting epithelia. Although CFTR is rapidly endocytosed from the apical membrane of polarized epithelial cells and efficiently recycled back to the plasma membrane, little is known about the molecular mechanisms ... More
The reversion-inducing cysteine-rich protein with Kazal motifs (RECK) interacts with membrane type 1 matrix metalloproteinase and CD13/aminopeptidase N and modulates their endocytic pathways.
AuthorsMiki T, Takegami Y, Okawa K, Muraguchi T, Noda M, Takahashi C
JournalJ Biol Chem
PubMed ID17329256
The reversion-inducing cysteine-rich protein with Kazal motifs (RECK) is anchored to the cell surface via glycosylphosphatidylinositol. This molecule antagonizes the function of membrane type 1 matrix metalloproteinase (MT1-MMP) to promote proMMP-2 maturation. Here, we attempt to clarify the mechanism underlying RECK functions. First, we found that RECK forms a complex ... More
ESCRT-III Acts Downstream of MLKL to Regulate Necroptotic Cell Death and Its Consequences.
Authors
JournalCell
PubMed ID28388412
SNX19 restricts endolysosome motility through contacts with the endoplasmic reticulum.
Authors
JournalNat Commun
PubMed ID34315878
Rigidity percolation uncovers a structural basis for embryonic tissue phase transitions.
Authors
JournalCell
PubMed ID33730596
Regulation of cytokine signaling through direct interaction between cytokine receptors and the ATG16L1 WD40 domain.
Authors
JournalNat Commun
PubMed ID33219218
Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network.
Authors
JournalCell Stem Cell
PubMed ID31303547
Tumor-Derived Extracellular Vesicles Breach the Intact Blood-Brain Barrier via Transcytosis.
Authors
JournalACS Nano
PubMed ID31479239
Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration.
Authors
JournalCell
PubMed ID32437660
TDP-43 Depletion in Microglia Promotes Amyloid Clearance but Also Induces Synapse Loss.
Authors
JournalNeuron
PubMed ID28669544
Fate restriction and multipotency in retinal stem cells.
Authors
JournalCell Stem Cell
PubMed ID22136930
In vivo cell biological screening identifies an endocytic capture mechanism for T-tubule formation.
Authors
JournalNat Commun
PubMed ID32709891
Spinal Interneurons With Dual Axon Projections to Knee-Extensor and Hip-Extensor Motor Pools.
AuthorsNguyen KH, Scheurich TE, Gu T, Berkowitz A
JournalFront Neural Circuits
PubMed ID32226362
The central nervous system (CNS) may simplify control of limb movements by activating certain combinations of muscles together, i.e., muscle synergies. Little is known, however, about the spinal cord interneurons that activate muscle synergies by exciting sets of motoneurons for different muscles. The turtle spinal cord, even without brain inputs ... More
Reverse Genetic Approaches to Investigate the Neurobiology of the Cnidarian Sea Anemone Nematostella vectensis.
AuthorsHavrilak JA, Layden MJ
JournalMethods Mol Biol
PubMed ID31552647
The cnidarian sea anemone Nematostella vectensis has grown in popularity as a model system to complement the ongoing work in traditional bilaterian model species (e.g. Drosophila, C. elegans, vertebrate). The driving force behind developing cnidarian model systems is the potential of this group of animals to impact EvoDevo studies aimed ... More
In vitro investigation of hyaluronan-based polymeric micelles for drug delivery into the skin: The internalization pathway.
AuthorsStarigazdová J, Nešporová K, Cepa M, Šínová R, Šmejkalová D, Huerta-Angeles G, Velebný V
JournalEur J Pharm Sci
PubMed ID31783157
In our previous research, we concluded that polymeric micelles based on hyaluronic acid are able to penetrate into the deeper layers of skin tissue. The aim of this work was to characterize the mechanisms involved in the uptake by skin cells, which is important for understanding the influence of the ... More