Phosphate-specific fluorescence labeling with BO-IMI: reaction details.
AuthorsWang P, Giese RW
JournalJ Chromatogr A
PubMed ID9677715
'Previously we reported than BO-IMI, a reagent which contains a BODIPY fluorophore linked to an imidazole group, can be used to covalently label a phosphomonoester in a single step under aqueous conditions [P. Wang, R.W. Giese, Anal. Chem. 65 (1993) 3518]. The reaction was conducted in the presence of a ... More
Determination of progesterone and 17-hydroxyprogesterone by high performance liquid chromatography after pre-column derivatization with 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionohydra zide.
AuthorsKatayama M, Nakane R, Matsuda Y, Kaneko S, Hara I, Sato H
JournalAnalyst
PubMed ID10396811
'Progesterone, 17-hydroxyprogesterone and four other 3-keto steroids were determined by high performance liquid chromatography with fluorescence detection. Each steroid was derivatized with 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene++ +-3- propionohydrazide (BODIPY FL hydrazide) and separated on a Wakosil 5C4 column with acetonitrile-water (7 + 3) as mobile phase. The limits of detection of progesterone, 17-hydroxyprogesterone, ... More
Hydrazide as a ligand moiety in immobilized metal ion affinity chromatography. Separation of BO-IMI and BODIPY-hydrazide.
AuthorsShen X, Giese RW
JournalJ Chromatogr A
PubMed ID9299723
BODIPY hydrazide (BO-HZ, a commercially available fluorescent dye) and BO-IMI (obtained by coupling the hydrazide moiety of BODIPY to the carboxyl group of N-acetylhistidine) were separated on three forms of a Sepharose-iminodiacetic acid column: Cu(II), Ni(II) and Zn(II). Whereas BO-IMI eluted first on the Cu(II) and Ni(II) columns (a pH ... More
Decreased uptake of bodipy-labelled compounds in the presence of the nuclear stain, DRAQ5.
AuthorsSnyder DS, Garon CF
JournalJ Microsc
PubMed ID12950469
We have found the nuclear stain DRAQ5 to decrease the cellular uptake of a series of boron dipyrromethane (bodipy)-labelled compounds. This phenomenon is consistent between Lysotracker Green DND 26, Lysotracker Red DND 99 and bodipy-labelled mycolactone. Although DRAQ5 uptake was not prevented, DRAQ5 was in significant excess in each case. ... More
Hyaluronic acid-N-hydroxysuccinimide: a useful intermediate for bioconjugation.
AuthorsLuo Y, Prestwich GD
JournalBioconjug Chem
PubMed ID11716704
Hyaluronic acid (HA) is an abundant nonsulfated glycosaminoglycan component of synovial fluid and the extracellular matrix. HA is an important building block for biocompatible and biointeractive materials with applications in drug delivery, tissue engineering, wound repair, and viscosupplementation. Herein we describe the synthesis and characterization of HA-N-succinimide, an activated ester ... More
Synthesis and selective cytotoxicity of a hyaluronic acid-antitumor bioconjugate.
AuthorsLuo Y, Prestwich GD
JournalBioconjug Chem
PubMed ID10502340
A cell-targeted prodrug was developed for the anti-cancer drug Taxol, using hyaluronic acid (HA) as the drug carrier. HA-Taxol bioconjugates were synthesized by linking the Taxol 2'-OH via a succinate ester to adipic dihydrazide-modified HA (HA-ADH). The coupling of Taxol-NHS ester and HA-ADH provided several HA bioconjugates with different levels ... More
Iron-dependent generation of free radicals: plausible mechanisms in the progressive deterioration of the temporomandibular joint.
AuthorsZardeneta G, Milam SB, Schmitz JP
JournalJ Oral Maxillofac Surg
PubMed ID10716113
PURPOSE: The purposes of this study were 1) to determine whether iron concentrations detected in temporomandibular joint (TMJ) lavage fluid samples obtained from symptomatic patients are sufficient to catalyze the degradation of specific extracellular matrix (ECM) molecules in vitro, and 2) to provide evidence of oxidative stress in symptomatic TMJs ... More