Dihydrorhodamine 123 - FAQs

查看更多产品信息 Dihydrorhodamine 123 - FAQs (D23806)

2 个常见问题解答

I am using the reactive oxygen species detection dye, Dihydrorhodamine 123, and when I put the dye in a solution of water and illuminate with UV light, I see a quick change in signal, even without cells. What is happening and how can I stop this?

You cannot stop it. Any time you illuminate a dye, including this one, photodegradation of the dye will lead to the production of singlet oxygen and free radicals. This in turn causes many ROS indicators, like this one, to become oxidized. If you are testing ROS using UV illumination, all you can do is use this as a baseline and subtract the signal from the sample with cells. The best you can do is reduce the illumination intensity (though this will also reduce the specific signal emission).

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What is the excitation and emission wavelength for rhodamine?

Rhodamine is a generic term for a wide variety of cationic dyes whose fluorescence emission can range from green, orange to red. The table below lists the excitation and emission maxima (nm), as well as molar extinction coefficients (“EC”; cm^-1 M^-1), for various rhodamine dyes (data derived with dye dissolved in methanol).

Dye	Excitation	Emission	EC
Rhodamine B	568	583	88,000
Rhodamine 123	507	529	101,000
Rhodamine 110	499	521	92,000
Rhodamine 6G	528	551	105,000
XRITC	572	596	92,000

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.