DAF-FM（4-氨基-5-甲基氨基-2',7'-二氟荧光素，以下简称 DAF-FM）
DAF-FM（4-氨基-5-甲基氨基-2',7'-二氟荧光素，以下简称 DAF-FM）
引用和文献 (24)
Invitrogen™

DAF-FM（4-氨基-5-甲基氨基-2',7'-二氟荧光素，以下简称 DAF-FM）

DAF-FM 是用于检测和定量低浓度一氧化氮 (NO) 的试剂。DAF-FM 本身不发荧光，但与 NO 反应后形成荧光产物苯并三唑。能检测荧光素的仪器均可以检测 DAF-FM了解更多信息
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货号数量
D238411 mg
货号 D23841
价格（CNY)
11,818.00
Each
添加至购物车
数量:
1 mg
价格（CNY)
11,818.00
Each
添加至购物车
DAF-FM 是用于检测和定量低浓度一氧化氮 (NO) 的试剂。DAF-FM 本身不发荧光，但与 NO 反应后形成荧光产物苯并三唑。能检测荧光素的仪器均可以检测 DAF-FM 荧光，包括流式细胞仪、显微镜、荧光酶标仪和荧光计。

DAF-FM 质量标准：
•DAF-FM Ex/EM：∼495/515 nm
• 冻干品应使用 DMSO 溶解，然后用水性缓冲液稀释，制成工作溶液
• 可以通过膜片钳移液管将 DAF-FM 加压注射或灌注进细胞中（如果您需要细胞渗透剂，请选择 DAF-FM 二乙酸酯）
• 含牛血清白蛋白 (BSA) 和酚红的缓冲液可能会影响荧光，应谨慎使用
• DAF-FM 的荧光量子产率为 ∼0.005，但与 NO 反应后产率达到 ∼0.81，增加了约 160 倍

DAF-FM 应用：
• 通过流式细胞分析评估转醛醇酶缺陷淋巴母细胞中 NO 的产生
• 检测神经营养因子刺激后胚胎皮层神经元中 NO 的蓄积
• 斑马鱼中 NO 的体内成像
• 活体显微镜检测与小鼠血管新生相关的 NO 生成
• 定量测定兔血小板中 ATP 诱导的 NO 释放

DAF-FM 相对于 DAF-2 的明显优势
DAF-FM 的 NO 加合物光谱与 pH 值高于 5.5 无关。此外，DAF-FM 的 NO 加合物的光稳定性显著高于 DAF-2，这意味着需要延长图像采集时间。总之，DAF-FM 试剂对 NO 的灵敏度高于 DAF-2（对于 DAF-FM，NO 的检测限 ∼3 nM；对于 DAF-2，NO 的检测限 ∼5 nM）。

一氧化氮研究用探针
我们提供了多种检测活性氧簇 (ROS) 和一氧化氮 (NO) 的产品。可在 Molecular Probes™ 手册第 18.3 节—一氧化氮检测用探针中查找这些产品。

仅供科研使用。不可用于人或动物的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
数量1 mg
产品类型NO Indicator
Unit SizeEach

引用和文献 (24)

引用和文献
Abstract
A stress surveillance system based on calcium and nitric oxide in marine diatoms.
Authors:Vardi A, Formiggini F, Casotti R, De Martino A, Ribalet F, Miralto A, Bowler C
Journal:PLoS Biol
PubMed ID:16475869
'Diatoms are an important group of eukaryotic phytoplankton, responsible for about 20% of global primary productivity. Study of the functional role of chemical signaling within phytoplankton assemblages is still in its infancy although recent reports in diatoms suggest the existence of chemical-based defense strategies. Here, we demonstrate how the accurate ... More
Nitric oxide-dependent mitochondrial biogenesis generates Ca2+ signaling profile of lupus T cells.
Authors:Nagy G, Barcza M, Gonchoroff N, Phillips PE, Perl A
Journal:J Immunol
PubMed ID:15356113
'Abnormal T cell activation and cell death underlie the pathology of systemic lupus erythematosus. Although mitochondrial hyperpolarization (MHP) represents an early and reversible checkpoint of T cell activation and apoptosis, lupus T cells exhibit persistent MHP. NO has recently been recognized as a key signal of mitochondrial biogenesis and mediator ... More
Perfusate composition influences nitric oxide homeostasis in rat juxtamedullary afferent arterioles.
Authors:Pittner J, Wolgast M, Persson AE
Journal:Acta Physiol Scand
PubMed ID:12940942
'AIM: Vascular diameters in isolated juxtamedullary nephron preparations perfused with cell-free solutions differ from those perfused with blood. In the present study, the effects of the albumin content of the perfusate on the afferent arteriolar diameter and endothelial nitric oxide were investigated in the isolated juxtamedullary nephron preparation perfused with ... More
Mitochondrial nitric oxide synthase is not eNOS, nNOS or iNOS.
Authors:Lacza Z, Snipes JA, Zhang J, Horváth EM, Figueroa JP, Szabó C, Busija DW
Journal:Free Radic Biol Med
PubMed ID:14607521
'Recent studies indicated that there is a distinct mitochondrial nitric oxide synthase (mtNOS) enzyme, which may be identical to the other known NOS isoforms. We investigated the possible involvement of the endothelial, the neuronal, and the inducible NOS isoforms (eNOS, nNOS, iNOS, respectively) in mitochondrial NO production. Mouse liver mitochondria ... More
Nitric oxide does not trigger early programmed cell death events but may contribute to cell-to-cell signaling governing progression of the Arabidopsis hypersensitive response.
Authors:Zhang C, Czymmek KJ, Shapiro AD
Journal:Mol Plant Microbe Interact
PubMed ID:14601664
'Nitric oxide (NO) has been suggested to play a role in the hypersensitive response (HR). Single- and double-label fluorescence microscopy experiments were conducted using Arabidopsis leaves infected with Pseudomonas syringae pv. tomato DC3000 carrying either avrB or avrRpt2. Kinetics of NO production were followed by measurement of green 4-amino-5-methylamino-2'',7''-difluorofluorescein (DAF-FM) ... More
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