DAF-FM 二乙酸酯（4-氨基-5-甲氨基-2',7'-二氟荧光素二乙酸酯）
DAF-FM 二乙酸酯（4-氨基-5-甲氨基-2',7'-二氟荧光素二乙酸酯）
引用和文献 (48)
Invitrogen™

DAF-FM 二乙酸酯（4-氨基-5-甲氨基-2',7'-二氟荧光素二乙酸酯）

DAF-FM 是用于检测和定量低浓度一氧化氮 (NO) 的试剂。DAF-FM 本身不发荧光，但与 NO 反应后形成荧光产物苯并三唑。能检测荧光素的仪器均可以检测 DAF-FM了解更多信息
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货号数量
D238421 mg
D2384410 x 50 μg
货号 D23842
价格（CNY)
12,320.00
Each
添加至购物车
数量:
1 mg
价格（CNY)
12,320.00
Each
添加至购物车
DAF-FM 是用于检测和定量低浓度一氧化氮 (NO) 的试剂。DAF-FM 本身不发荧光，但与 NO 反应后形成荧光产物苯并三唑。能检测荧光素的仪器均可以检测 DAF-FM 荧光，包括流式细胞仪、显微镜、荧光酶标仪和荧光计。

DAF-FM 质量标准：
•DAF-FM Ex/EM：∼495/515 nm
• 冻干品应使用 DMSO 溶解，然后用水性缓冲液稀释，制成工作溶液
• DAF-FM 二乙酸酯具有细胞可透过性并且能够被动穿透细胞膜；一旦进入细胞，它就会转换为细胞不可透过形式
• 含牛血清白蛋白 (BSA) 和酚红的缓冲液可能会影响荧光，应谨慎使用
• DAF-FM 的荧光量子产率为 ∼0.005，但与 NO 反应后产率达到 ∼0.81 左右，约增加了 160 倍

DAF-FM 的应用：
• 通过流式细胞分析评估转醛醇酶缺陷淋巴母细胞中 NO 的产生
• 检测神经营养因子刺激后胚胎皮层神经元中 NO 的蓄积
• 斑马鱼中 NO 的体内成像
• 活体显微镜检测与小鼠血管新生相关的 NO 生成
• 定量测定兔血小板中 ATP 诱导的 NO 释放

DAF-FM 相对于 DAF-2 的明显优势
DAF-FM 的 NO 加合物光谱与 pH 值高于 5.5 无关。此外，DAF-FM 的 NO 加合物的光稳定性显著高于 DAF-2，这意味着需要延长图像采集时间。总之，DAF-FM 试剂对 NO 的灵敏度高于 DAF-2（对于 DAF-FM，NO 的检测限 ∼3 nM；对于 DAF-2，NO 的检测限 ∼5 nM）。

一氧化氮研究用探针
我们提供了多种检测活性氧簇 (ROS) 和一氧化氮 (NO) 的产品。可在 Molecular Probes™ 手册第 18.3 节—一氧化氮检测用探针中查找这些产品。

仅供科研使用。不可用于人或动物的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
数量1 mg
产品类型NO Indicator
Unit SizeEach

引用和文献 (48)

引用和文献
Abstract
A stress surveillance system based on calcium and nitric oxide in marine diatoms.
Authors:Vardi A, Formiggini F, Casotti R, De Martino A, Ribalet F, Miralto A, Bowler C
Journal:PLoS Biol
PubMed ID:16475869
'Diatoms are an important group of eukaryotic phytoplankton, responsible for about 20% of global primary productivity. Study of the functional role of chemical signaling within phytoplankton assemblages is still in its infancy although recent reports in diatoms suggest the existence of chemical-based defense strategies. Here, we demonstrate how the accurate ... More
Nitric oxide-dependent mitochondrial biogenesis generates Ca2+ signaling profile of lupus T cells.
Authors:Nagy G, Barcza M, Gonchoroff N, Phillips PE, Perl A
Journal:J Immunol
PubMed ID:15356113
'Abnormal T cell activation and cell death underlie the pathology of systemic lupus erythematosus. Although mitochondrial hyperpolarization (MHP) represents an early and reversible checkpoint of T cell activation and apoptosis, lupus T cells exhibit persistent MHP. NO has recently been recognized as a key signal of mitochondrial biogenesis and mediator ... More
Fluorescence monitoring of ATP-stimulated, endothelium-derived nitric oxide production in channels of a poly(dimethylsiloxane)-based microfluidic device.
Authors:D'Amico Oblak T, Root P, Spence DM
Journal:Anal Chem
PubMed ID:16643013
'Intracellular nitric oxide (NO) production in a microfluidic endothelium is detected using fluorescence microscopy. Bovine pulmonary artery endothelial cells (bPAECs) were loaded with the fluorescence probe diaminodifluorofluorescein diacetate (DAF-FM DA), and the subsequent fluorescent DAF-FM DA/NO adduct was measured. Solutions of bradykinin, a well-known stimulus of endothelium-derived NO, activated nitric ... More
Carbohydrate oxidation acidifies endosomes, regulating antigen processing and TLR9 signaling.
Authors:Lewis CJ, Cobb BA,
Journal:J Immunol
PubMed ID:20200279
'Phagocytes kill encapsulated microbes through oxidative cleavage of surface carbohydrates, releasing glycan fragments and microbial contents that serve as ligands for immune receptors, which tailor the immune response against the offending pathogen. The glycan fragments serve as MHC class II (MHC II) ligands and innate receptor agonists, whereas microbial proteins ... More
Real-time measurement of nitric oxide in single mature mouse skeletal muscle fibres during contractions.
Authors:Pye D, Palomero J, Kabayo T, Jackson MJ
Journal:J Physiol
PubMed ID:17331997
'Nitric oxide (NO) is thought to play multiple roles in skeletal muscle including regulation of some adaptations to contractile activity, but appropriate methods for the analysis of intracellular NO activity are lacking. In this study we have examined the intracellular generation of NO in isolated single mature mouse skeletal muscle ... More
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