A stress surveillance system based on calcium and nitric oxide in marine diatoms.
AuthorsVardi A, Formiggini F, Casotti R, De Martino A, Ribalet F, Miralto A, Bowler C
JournalPLoS Biol
PubMed ID16475869
'Diatoms are an important group of eukaryotic phytoplankton, responsible for about 20% of global primary productivity. Study of the functional role of chemical signaling within phytoplankton assemblages is still in its infancy although recent reports in diatoms suggest the existence of chemical-based defense strategies. Here, we demonstrate how the accurate ... More
Nitric oxide-dependent mitochondrial biogenesis generates Ca2+ signaling profile of lupus T cells.
AuthorsNagy G, Barcza M, Gonchoroff N, Phillips PE, Perl A
JournalJ Immunol
PubMed ID15356113
'Abnormal T cell activation and cell death underlie the pathology of systemic lupus erythematosus. Although mitochondrial hyperpolarization (MHP) represents an early and reversible checkpoint of T cell activation and apoptosis, lupus T cells exhibit persistent MHP. NO has recently been recognized as a key signal of mitochondrial biogenesis and mediator ... More
Fluorescence monitoring of ATP-stimulated, endothelium-derived nitric oxide production in channels of a poly(dimethylsiloxane)-based microfluidic device.
AuthorsD'Amico Oblak T, Root P, Spence DM
JournalAnal Chem
PubMed ID16643013
'Intracellular nitric oxide (NO) production in a microfluidic endothelium is detected using fluorescence microscopy. Bovine pulmonary artery endothelial cells (bPAECs) were loaded with the fluorescence probe diaminodifluorofluorescein diacetate (DAF-FM DA), and the subsequent fluorescent DAF-FM DA/NO adduct was measured. Solutions of bradykinin, a well-known stimulus of endothelium-derived NO, activated nitric ... More
Carbohydrate oxidation acidifies endosomes, regulating antigen processing and TLR9 signaling.
AuthorsLewis CJ, Cobb BA,
JournalJ Immunol
PubMed ID20200279
'Phagocytes kill encapsulated microbes through oxidative cleavage of surface carbohydrates, releasing glycan fragments and microbial contents that serve as ligands for immune receptors, which tailor the immune response against the offending pathogen. The glycan fragments serve as MHC class II (MHC II) ligands and innate receptor agonists, whereas microbial proteins ... More
Real-time measurement of nitric oxide in single mature mouse skeletal muscle fibres during contractions.
AuthorsPye D, Palomero J, Kabayo T, Jackson MJ
JournalJ Physiol
PubMed ID17331997
'Nitric oxide (NO) is thought to play multiple roles in skeletal muscle including regulation of some adaptations to contractile activity, but appropriate methods for the analysis of intracellular NO activity are lacking. In this study we have examined the intracellular generation of NO in isolated single mature mouse skeletal muscle ... More
Phosphoaspirin (MDC-43), a novel benzyl ester of aspirin, inhibits the growth of human cancer cell lines more potently than aspirin: a redox-dependent effect.
'Aspirin is chemopreventive against colon and probably other cancers, but this effect is relatively weak and its chronic administration to humans is associated with significant side effects. Because of these limitations, extensive effort has been exerted to improve the pharmacological properties of aspirin. We have determined the anticancer activity and ... More
Examining intracellular organelle function using fluorescent probes: from animalcules to quantum dots.
AuthorsZorov DB, Kobrinsky E, Juhaszova M, Sollott SJ
JournalCirc Res
PubMed ID15297386
'Fluorescence microscopy imaging has become one of the most useful techniques to assess the activity of individual cells, subcellular trafficking of signals to and between organelles, and to appreciate how organelle function is regulated. The past 2 decades have seen a tremendous advance in the rational design and development in ... More
Multimodality imaging of nitric oxide and nitric oxide synthases.
AuthorsHong H, Sun J, Cai W,
JournalFree Radic Biol Med
PubMed ID19524664
'Nitric oxide (NO) and NO synthases (NOSs) are crucial factors in many pathophysiological processes such as inflammation, vascular/neurological function, and many types of cancer. Noninvasive imaging of NO or NOS can provide new insights in understanding these diseases and facilitate the development of novel therapeutic strategies. In this review, we ... More
Fluorescent Indicators for Imaging Nitric Oxide Production.
AuthorsKojima H, Urano Y, Kikuchi K, Higuchi T, Hirata Y, Nagano T
JournalAngew Chem Int Ed Engl
PubMed ID10556905
'The membrane-permeating indicator DAF-FM DA is transformed by intracellular esterases into the highly water-soluble dye DAF-FM, which traps NO produced by NO synthase (NOS) to yield a highly fluorescent triazole compound in cells (see schematic diagram). Monitoring with a fluorescence microscope should allow direct identification of intracellular production and location ... More
What is the real physiological NO concentration in vivo?
AuthorsHall CN, Garthwaite J,
JournalNitric Oxide
PubMed ID19602444
'Clarity about the nitric oxide (NO) concentrations existing physiologically is essential for developing a quantitative understanding of NO signalling, for performing experiments with NO that emulate reality, and for knowing whether or not NO concentrations become abnormal in disease states. A decade ago, a value of about 1 microM seemed ... More
Regulation of beta cell glucokinase by S-nitrosylation and association with nitric oxide synthase.
AuthorsRizzo MA, Piston DW
JournalJ Cell Biol
PubMed ID12707306
'Glucokinase (GK) activity plays a key role in glucose-stimulated insulin secretion from pancreatic beta cells. Insulin regulates GK activity by modulating its association with secretory granules, although little is known about the mechanisms involved in regulating this association. Using quantitative imaging of multicolor fluorescent proteins fused to GK, we found ... More
Direct effect of cholesterol on insulin secretion: a novel mechanism for pancreatic beta-cell dysfunction.
AuthorsHao M, Head WS, Gunawardana SC, Hasty AH, Piston DW,
JournalDiabetes
PubMed ID17575085
'OBJECTIVE: Type 2 diabetes is often accompanied by abnormal blood lipid and lipoprotein levels, but most studies on the link between hyperlipidemia and diabetes have focused on free fatty acids (FFAs). In this study, we examined the relationship between cholesterol and insulin secretion from pancreatic beta-cells that is independent of ... More
Neuronal nitric oxide synthase: its role and regulation in macula densa cells.
AuthorsKovács G, Komlósi P, Fuson A, Peti-Peterdi J, Rosivall L, Bell PD
JournalJ Am Soc Nephrol
PubMed ID14514725
'Macula densa (MD) cells detect changes in distal tubular sodium chloride concentration ([NaCl](L)), at least in part, through an apical Na:2Cl:K co-transporter. This co-transporter may be a site for regulation of tubuloglomerular feedback (TGF), and recently angiotensin II (Ang II) was shown to regulate the MD Na:2Cl:K co-transporter. In addition, ... More
Fluorescent probes to investigate nitric oxide and other reactive nitrogen species in biology (truncated form: fluorescent probes of reactive nitrogen species).
AuthorsMcQuade LE, Lippard SJ,
JournalCurr Opin Chem Biol
PubMed ID19926519
'Reactive nitrogen species (RNS), such as nitric oxide and its higher oxides, play important roles in cell signaling during many physiological and pathological events. Elucidation of the exact functions of these important biomolecules has been hampered by the inability to detect RNS reliably under biological conditions. A surge of research ... More
On the specificity of 4-amino-5-methylamino-2',7'-difluorofluorescein as a probe for nitric oxide.
AuthorsBalcerczyk A, Soszynski M, Bartosz G
JournalFree Radic Biol Med
PubMed ID15993331
The specificity of 4-amino-5-methylamino-2',7'-difluorofluorescein (DAF-FM) for nitric oxide was evaluated in in vitro systems. The probe was found fairly specific for nitric oxide. Potential sources of artifacts include the autoxidation of DAF-FM, potentiated by light, and its oxidation by sources of superoxide and peroxyl radicals, leading to fluorescence spectra indistinguishable ... More
Fluorescent and luminescent probes for measurement of oxidative and nitrosative species in cells and tissues: progress, pitfalls, and prospects.
AuthorsWardman P,
JournalFree Radic Biol Med
PubMed ID17761297
Chemical probes for free radicals in biology are important tools; fluorescence and chemiluminescence offer high detection sensitivity. This article reviews progress in the development of probes for "reactive oxygen and nitrogen" species, emphasizing the caution needed in their use. Reactive species include hydrogen peroxide; hydroxyl, superoxide, and thiyl radicals; carbonate ... More
Microscopic technique for the detection of nitric oxide-dependent angiogenesis in an animal model.
AuthorsNamkoong S, Chung BH, Ha KS, Lee H, Kwon YG, Kim YM,
JournalMethods Enzymol
PubMed ID18554547
Nitric oxide (NO) plays an important role in maintaining vascular homeostasis. The importance of NO in the vasculature is demonstrated by several experimental conditions, such as vascular endothelial growth factor (VEGF)-induced angiogenesis. Thus, the NO metabolic pathway in endothelial cells could be one of the main contributing factors for angiogenesis. ... More
Glucose, insulin, and leptin signaling pathways modulate nitric oxide synthesis in glucose-inhibited neurons in the ventromedial hypothalamus.
AuthorsCanabal DD, Song Z, Potian JG, Beuve A, McArdle JJ, Routh VH,
JournalAm J Physiol Regul Integr Comp Physiol
PubMed ID17170237
Glucose-sensing neurons in the ventromedial hypothalamus (VMH) are involved in the regulation of glucose homeostasis. Glucose-sensing neurons alter their action potential frequency in response to physiological changes in extracellular glucose, insulin, and leptin. Glucose-excited neurons decrease, whereas glucose-inhibited (GI) neurons increase, their action potential frequency when extracellular glucose is reduced. ... More
S-Nitrosylation of histone deacetylase 2 induces chromatin remodelling in neurons.
AuthorsNott A, Watson PM, Robinson JD, Crepaldi L, Riccio A,
JournalNature
PubMed ID18754010
Brain-derived neurotrophic factor (BDNF) and other neurotrophins have a vital role in the development of the rat and mouse nervous system by influencing the expression of many specific genes that promote differentiation, cell survival, synapse formation and, later, synaptic plasticity. Although nitric oxide (NO) is known to be an important ... More
Glutathione depletion is necessary for apoptosis in lymphoid cells independent of reactive oxygen species formation.
AuthorsFranco R, Panayiotidis MI, Cidlowski JA,
JournalJ Biol Chem
PubMed ID17724027
Changes in the intracellular redox environment of cells have been reported to be critical for the activation of apoptotic enzymes and the progression of programmed cell death. Glutathione (GSH) depletion is an early hallmark observed in apoptosis, and we have demonstrated that GSH efflux during death receptor-mediated apoptosis occurs via ... More
TRPM7 activates m-calpain by stress-dependent stimulation of p38 MAPK and c-Jun N-terminal kinase.
TRPM7 is a Ca(2)(+)-permeant and Mg(2)(+)-permeant ion channel in possession of its own kinase domain. In a previous study, we showed that overexpression of the channel-kinase in HEK-293 cells produced cell rounding and loss of adhesion, which was dependent on the Ca(2+)-dependent protease m-calpain. The TRPM7-elicited change in cell morphology ... More
Transaldolase deficiency influences the pentose phosphate pathway, mitochondrial homoeostasis and apoptosis signal processing.
AuthorsQian Y, Banerjee S, Grossman CE, Amidon W, Nagy G, Barcza M, Niland B, Karp DR, Middleton FA, Banki K, Perl A,
JournalBiochem J
PubMed ID18498245
TAL (transaldolase) was originally described in the yeast as an enzyme of the PPP (pentose phosphate pathway). However, certain organisms and mammalian tissues lack TAL, and the overall reason for its existence is unclear. Recently, deletion of Ser(171) (TALDeltaS171) was found in five patients causing inactivation, proteasome-mediated degradation and complete ... More
Nitric oxide mediates T cell cytokine production and signal transduction in histidine decarboxylase knockout mice.
AuthorsKoncz A, Pasztoi M, Mazan M, Fazakas F, Buzas E, Falus A, Nagy G,
JournalJ Immunol
PubMed ID17982051
Histamine is a key regulator of the immune system. Several lines of evidence suggest the role of histamine in T cell activation and accelerated Th1 immune response is a hallmark of histidine decarboxylase knockout (HDC-KO) mice, with a complete lack of endogenously produced histamine. According to our previous work, T ... More
Ca2+ -linked upregulation and mitochondrial production of nitric oxide in the mouse preimplantation embryo.
AuthorsManser RC, Houghton FD
JournalJ Cell Sci
PubMed ID16638811
Previous studies have demonstrated a role for the signalling agent nitric oxide in regulating preimplantation embryo development. We have now investigated the biochemical mode of action of nitric oxide in mouse embryos in terms of mitochondrial function and Ca2+ signalling. DETA-NONOate, a nitric oxide donor, decreased day 4 blastocyst cell ... More
An iron-induced nitric oxide burst precedes ubiquitin-dependent protein degradation for Arabidopsis AtFer1 ferritin gene expression.
AuthorsArnaud N, Murgia I, Boucherez J, Briat JF, Cellier F, Gaymard F
JournalJ Biol Chem
PubMed ID16782706
Ferritins play an essential role in iron homeostasis by sequestering iron in a bioavailable and non-toxic form. In plants, ferritin mRNAs are highly and quickly accumulated in response to iron overload. Such accumulation leads to a subsequent ferritin protein synthesis and iron storage, thus avoiding oxidative stress to take place. ... More
Determination and bioimaging method for nitric oxide in biological specimens by diaminofluorescein fluorometry.
AuthorsItoh Y, Ma FH, Hoshi H, Oka M, Noda K, Ukai Y, Kojima H, Nagano T, Toda N
JournalAnal Biochem
PubMed ID11112265
A simple and sensitive assay and a cellular bioimaging method for nitric oxide (NO) were developed using a novel diaminofluorescein DAF-FM and its diacetate. DAF-FM is converted via an NO-specific mechanism to an intensely fluorescent triazole derivative. For the measurement of NO, the triazole derivative of DAF-FM was determined by ... More
Interactions among nitric oxide and Bcl-family proteins after MPP+ exposure of SH-SY5Y neural cells I: MPP+ increases mitochondrial NO and Bax protein.
AuthorsDennis J, Bennett JP
JournalJ Neurosci Res
PubMed ID12645081
We studied effects of methylpyridinium ion (MPP(+)) on apoptosis, cell death and regulation of Bcl-2-family proteins in SH-SY5Y neuroblastoma cells. MPP(+) increased intracellular accumulation of DNA-histone complexes as a measure of apoptosis and decreased intracellular calcein fluorescence as a measure of cell death. If ATP synthesis was supported, MPP(+) caused ... More
Synthesis of nitric oxide in postganglionic myenteric neurons during endotoxemia: implications for gastric motor function in rats.
AuthorsQuintana E, Hernández C, Alvarez-Barrientos A, Esplugues JV, Barrachina MD
JournalFASEB J
PubMed ID14715697
We have investigated the mechanisms underlying acute changes in gastric motor function triggered by endotoxemia. In fundal strips from rats pre-treated with endotoxin (40 microg/kg, i.p. 30 min), mechanical activity was analyzed and the source of nitric oxide (NO) was visualized by confocal microscopy of tissue loaded with the fluorescent ... More
Stretch activates nitric oxide production in pulmonary vascular endothelial cells in situ.
AuthorsKuebler WM, Uhlig U, Goldmann T, Schael G, Kerem A, Exner K, Martin C, Vollmer E, Uhlig S
JournalAm J Respir Crit Care Med
PubMed ID12947026
Whereas endothelial responses to shear stress have been studied extensively, the responses to circumferential vascular stretch are yet poorly defined. Circumferential stretch in pulmonary microvessels is largely determined by the transmural pressure gradient, hence by both vascular perfusion and alveolar ventilation pressures. Here, we have studied the production of nitric ... More
Tetrahydrobiopterin, a critical factor in the production and role of nitric oxide in mast cells.
AuthorsGilchrist M, Hesslinger C, Befus AD
JournalJ Biol Chem
PubMed ID14514683
Mast cells (MC) are biologically potent, ubiquitously distributed immune cells with fundamental roles in host integrity and disease. MC diversity and function is regulated by exogenous nitric oxide; however, the production and function of endogenously produced NO in MC is enigmatic. We used rat peritoneal MC (PMC) as an in ... More
Innate immunity in Arabidopsis thaliana: lipopolysaccharides activate nitric oxide synthase (NOS) and induce defense genes.
AuthorsZeidler D, Zähringer U, Gerber I, Dubery I, Hartung T, Bors W, Hutzler P, Durner J
JournalProc Natl Acad Sci U S A
PubMed ID15498873
Lipopolysaccharides (LPS) are cell-surface components of Gram-negative bacteria and are microbe-/pathogen-associated molecular patterns in animal pathosystems. As for plants, the molecular mechanisms of signal transduction in response to LPS are not known. Here, we show that Arabidopsis thaliana reacts to LPS with a rapid burst of NO, a hallmark of ... More
The NO pathway acts late during the fertilization response in sea urchin eggs.
AuthorsLeckie C, Empson R, Becchetti A, Thomas J, Galione A, Whitaker M
JournalJ Biol Chem
PubMed ID12540836
Both the inositol 1,4,5-trisphosphate (InsP(3)) and ryanodine receptor pathways contribute to the Ca(2+) transient at fertilization in sea urchin eggs. To date, the precise contribution of each pathway has been difficult to ascertain. Evidence has accumulated to suggest that the InsP(3) receptor pathway has a primary role in causing Ca(2+) ... More
Evidence that the vasodilator angiotensin-(1-7)-Mas axis plays an important role in erectile function.
Authorsda Costa Gonçalves AC, Leite R, Fraga-Silva RA, Pinheiro SV, Reis AB, Reis FM, Touyz RM, Webb RC, Alenina N, Bader M, Santos RA
JournalAm J Physiol Heart Circ Physiol
PubMed ID17616753
The vasodilator/antiproliferative peptide angiotensin-(1-7) [ANG-(1-7)] is released into the corpus cavernosum sinuses, but its role in erectile function has yet to be defined. In this study, we sought to determine whether ANG-(1-7) and its receptor Mas play a role in erectile function. The ANG-(1-7) receptor Mas was immunolocalized in rat ... More
Anti-D initially stimulates an Fc-dependent leukocyte oxidative burst and subsequently suppresses erythrophagocytosis via interleukin-1 receptor antagonist.
AuthorsCoopamah MD, Freedman J, Semple JW
JournalBlood
PubMed ID12829590
Previous results have demonstrated that anti-D therapy in children with chronic auto-immune thrombocytopenic purpura (AITP) induced a significant increase in several pro- and anti-inflammatory plasma cytokines within 2 hours of administration. To investigate the biologic basis of these early in vivo responses, we developed a flow cytometric assay to measure ... More
Effects of interferon beta on transcobalamin II-receptor expression and antitumor activity of nitrosylcobalamin.
AuthorsBauer JA, Morrison BH, Grane RW, Jacobs BS, Dabney S, Gamero AM, Carnevale KA, Smith DJ, Drazba J, Seetharam B, Lindner DJ
JournalJ Natl Cancer Inst
PubMed ID12096086
BACKGROUND: The ubiquitous plasma membrane transcobalamin II receptor (TC II-R) mediates uptake of cobalamin (Cbl; vitamin B12), an essential micronutrient. Tumors often require more Cbl than normal tissue, and increased Cbl uptake may result from increased TC II-R expression. To examine whether Cbl could therefore be used as a carrier ... More
AuthorsNath AK, Enciso J, Kuniyasu M, Hao XY, Madri JA, Pinter E
JournalDevelopment
PubMed ID15128676
Nitric oxide (NO) has been demonstrated to mediate events during ovulation, pregnancy, blastocyst invasion and preimplantation embryogenesis. However, less is known about the role of NO during postimplantation development. Therefore, in this study, we explored the effects of NO during vascular development of the murine yolk sac, which begins shortly ... More
Mitochondrial calcium uptake stimulates nitric oxide production in mitochondria of bovine vascular endothelial cells.
AuthorsDedkova EN, Ji X, Lipsius SL, Blatter LA
JournalAm J Physiol Cell Physiol
PubMed ID14512291
Although nitric oxide (NO) is a known modulator of cell respiration in vascular endothelium, the presence of a mitochondria-specific nitric oxide synthase (mtNOS) in these cells is still a controversial issue. We have used laser scanning confocal microscopy in combination with the NO-sensitive fluorescent dye DAF-2 to monitor changes in ... More
T cell activation-induced mitochondrial hyperpolarization is mediated by Ca2+- and redox-dependent production of nitric oxide.
AuthorsNagy G, Koncz A, Perl A
JournalJ Immunol
PubMed ID14607919
Activation, proliferation, or programmed cell death of T lymphocytes is regulated by the mitochondrial transmembrane potential (Deltapsi(m)) through controlling ATP synthesis, production of reactive oxygen intermediates (ROI), and release of cell death-inducing factors. Elevation of Deltapsi(m) or mitochondrial hyperpolarization is an early and reversible event associated with both T cell ... More
Hypothyroid phenotype is contributed by mitochondrial complex I inactivation due to translocated neuronal nitric-oxide synthase.
Although transcriptional effects of thyroid hormones have substantial influence on oxidative metabolism, how thyroid sets basal metabolic rate remains obscure. Compartmental localization of nitric-oxide synthases is important for nitric oxide signaling. We therefore examined liver neuronal nitric-oxide synthase-alpha (nNOS) subcellular distribution as a putative mechanism for thyroid effects on rat ... More
Odorant-evoked nitric oxide signals in the antennal lobe of Manduca sexta.
AuthorsCollmann C, Carlsson MA, Hansson BS, Nighorn A
JournalJ Neurosci
PubMed ID15240798
The gaseous signaling molecule nitric oxide (NO) can affect the activities of neurons and neural networks in many different systems. The strong expression of NO synthase (NOS) in the primary synaptic neuropil (the antennal lobe in insects and the olfactory bulb in vertebrates) of the olfactory system of most organisms, ... More
Role of NO in endothelin-regulated drug transport in the renal proximal tubule.
AuthorsNotenboom S, Miller DS, Smits P, Russel FG, Masereeuw R
JournalAm J Physiol Renal Physiol
PubMed ID11832426
We previously demonstrated in intact killifish renal proximal tubules that endothelin (ET), acting through an ET(B) receptor and protein kinase C (PKC), reduced transport mediated by multidrug resistance-associated protein 2 (Mrp2), i.e., luminal accumulation of fluorescein methotrexate (FL-MTX) (Masereeuw R, Terlouw SA, Van Aubel RAMH, Russel FGM, and Miller DS. ... More
A calcium-induced calcium influx factor, nitric oxide, modulates the refilling of calcium stores in astrocytes.
AuthorsLi N, Sul JY, Haydon PG
JournalJ Neurosci
PubMed ID14614089
The roles of nitric oxide are primarily undefined in astrocytes, cells that are active partners in synaptic transmission. Because nitric oxide synthases are present in astrocytes, we imaged the formation of nitric oxide in cultured murine cortical astrocytes using DAF-FM (4-amino-5-methylamino-2',7'-difluorofluorescein diacetate). We demonstrated that physiological concentrations of ATP induced ... More
Fluorescence determination of nitric oxide production in stimulated and activated platelets.
AuthorsKu CJ, Karunarathne W, Kenyon S, Root P, Spence D
JournalAnal Chem
PubMed ID17288406
Nitric oxide (NO) is quantitatively determined in platelets prior to, and after, stimulation with adenosine triphosphate (ATP) or activation with adenosine diphosphate (ADP). Platelets obtained from the whole blood of rabbits were loaded with the fluorescence probe diaminodifluorofluorescein diacetate (DAF-FM DA), and the subsequent NO production was measured as a ... More
Selective inhibition of human inducible nitric oxide synthase by S-alkyl-L-isothiocitrulline-containing dipeptides.
AuthorsPark JM, Higuchi T, Kikuchi K, Urano Y, Hori H, Nishino T, Aoki J, Inoue K, Nagano T
JournalBr J Pharmacol
PubMed ID11309260
The aim of this study was to investigate the structure-activity relationship of S-alkyl-L-isothiocitrulline-containing dipeptides towards three partially purified recombinant human nitric oxide synthase (NOS) isozymes, as well as the effects of these compounds on cytokine-induced NO production by human DLD-1 cells. In an in vitro assay, S-methyl-L-isothiocitrulline (L-MIT) was slightly ... More
Nitric oxide-mediated signaling in the bystander response of individually targeted glioma cells.
AuthorsShao C, Stewart V, Folkard M, Michael BD, Prise KM
JournalCancer Res
PubMed ID14679007
Bystander responses have been reported to be a major determinant of the response of cells to radiation exposure at low doses, including those of relevance to therapy. In this study, human glioblastoma T98G cell nuclei were individually irradiated with an exact number of helium ions using a single-cell microbeam. It ... More
Pertussis toxin activates L-arginine uptake in pulmonary endothelial cells through downregulation of PKC-alpha activity.
AuthorsZharikov SI, Krotova KY, Belayev L, Block ER
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID14695118
Pertussis toxin (PTX) induces activation of l-arginine transport in pulmonary artery endothelial cells (PAEC). The effects of PTX on l-arginine transport appeared after 6 h of treatment and reached maximal values after treatment for 12 h. PTX-induced changes in l-arginine transport were not accompanied by changes in expression of cationic ... More
Adrenomedullin stimulates nitric oxide production from primary rat hypothalamic neurons: roles of calcium and phosphatases.
AuthorsXu Y, Krukoff TL
JournalMol Pharmacol
PubMed ID17446268
Adrenomedullin (ADM) in the brain plays important roles in the maintenance of homeostasis. Although in vivo evidence has suggested that nitric oxide (NO) mediates ADM's effects in the brain, mechanisms for ADM stimulation of NO production in neurons have not been identified. In the present study, primary hypothalamic neurons were ... More
The cytosolic phospholipase A2 pathway, a safeguard of beta2-adrenergic cardiac effects in rat.
AuthorsAit-Mamar B, Cailleret M, Rucker-Martin C, Bouabdallah A, Candiani G, Adamy C, Duvaldestin P, Pecker F, Defer N, Pavoine C
JournalJ Biol Chem
PubMed ID15728587
We have recently demonstrated that in human heart, beta2-adrenergic receptors (beta2-ARs) are biochemically coupled not only to the classical adenylyl cyclase (AC) pathway but also to the cytosolic phospholipase A2 (cPLA2) pathway (Pavoine, C., Behforouz, N., Gauthier, C., Le Gouvello, S., Roudot-Thoraval, F., Martin, C. R., Pawlak, A., Feral, C., ... More
Angiopoietin-like 4 stimulates STAT3-mediated iNOS expression and enhances angiogenesis to accelerate wound healing in diabetic mice.
Authors
JournalMol Ther
PubMed ID24903577
Transient external force induces phenotypic reversion of malignant epithelial structures via nitric oxide signaling.
Authors
JournalElife
PubMed ID29560858
Sialic Acid-Siglec-E Interactions During Pseudomonas aeruginosa Infection of Macrophages Interferes With Phagosome Maturation by Altering Intracellular Calcium Concentrations.
Authors
JournalFront Immunol
PubMed ID32184783
Melanoma-associated fibroblasts impair CD8+ T cell function and modify expression of immune checkpoint regulators via increased arginase activity.
Authors
JournalCell Mol Life Sci
PubMed ID32328671
Laminar Flow on Endothelial Cells Suppresses eNOS O-GlcNAcylation to Promote eNOS Activity.
Authors
JournalCirc Res
PubMed ID34605247
Association of reactive oxygen and nitrogen intermediate and complement levels with apoptosis of peripheral blood mononuclear cells in lupus patients.
Authors
JournalArthritis Rheum
PubMed ID17968874
The Atr-Chek1 pathway inhibits axon regeneration in response to Piezo-dependent mechanosensation.
Authors
JournalNat Commun
PubMed ID34158506
Superoxide is an associated signal for apoptosis in axonal injury.
Authors
JournalBrain
PubMed ID20495185
Cytoplasmic condensation induced by membrane damage is associated with antibiotic lethality.