Schizosaccharomyces pombe cdc4+ gene encodes a novel EF-hand protein essential for cytokinesis.
AuthorsMcCollum D, Balasubramanian MK, Pelcher LE, Hemmingsen SM, Gould KL
JournalJ Cell Biol
PubMed ID7622565
'Schizosaccharomyces pombe cells divide by medial fission. One class of cell division mutants (cdc), the late septation mutants, defines four genes: cdc3, cdc4, cdc8, and cdc12 (Nurse, P., P. Thuriaux, and K. Nasmyth. 1976. Mol. & Gen. Genet. 146:167-178). We have cloned and characterized the cdc4 gene and show that ... More
A genetically engineered, protein-based optical biosensor of myosin II regulatory light chain phosphorylation.
AuthorsPost PL, Trybus KM, Taylor DL
JournalJ Biol Chem
PubMed ID8175704
Myosin II is an important motor in the contraction of smooth and striated muscle as well as in a variety of non-muscle cell motile events including cytokinesis, cortical contractions during migration of fibroblasts, and capping of receptors. Phosphorylation of the 20-kDa light chain by myosin light chain kinase is part ... More