Caged agonist of P2Y1 and P2Y12 receptors for light-directed facilitation of platelet aggregation.
AuthorsGao ZG, Hechler B, Besada P, Gachet C, Jacobson KA,
JournalBiochem Pharmacol
PubMed ID18199424
We have prepared a caged form (MRS2703) of a potent dual agonist of the P2Y(1) and P2Y(12) nucleotide receptors, 2-MeSADP, by blocking the beta-phosphate group with a 1-(3,4-dimethyloxyphenyl)eth-1-yl phosphoester. Although MRS2703 is itself inactive at human P2Y(1) and P2Y(12) receptors expressed heterologously in 1321N1 astrocytoma cells or in washed human ... More
An easily synthesized, photolyzable luciferase substrate for in vivo luciferase activity measurement.
AuthorsYang J, Thomason DB
JournalBiotechniques
PubMed ID8267979
'Many reporter gene assays require killing the cell by fixation or lysis. For assays in living cells, the substrate delivery is inefficient and cannot be supplied in situ in a bolus, which makes assays highly variable. We report a simple synthesis of a luciferin ester that is both photolyzable and ... More
Recovery of the oxidative activity of caged bovine haemoglobin after UV photolysis.
AuthorsBédouet L, Adenier H, Pulvin S, Bedel-Cloutour C, Thomas D,
JournalBiochem Biophys Res Commun
PubMed ID15240139
Caging of bovine haemoglobin with increasing amounts of 1-(2-nitrophenyl)ethyl (NPE) and uncaging after a 366 nm irradiation was examined. Caged and photolysed conjugates were characterised by enzymatic assay of the ABTS oxidation, UV/Vis absorbance, and electrospray mass ionisation. Modification of haemoglobin with 50, 75, and 100 equivalents of 1-(2-nitrophenyl)diazoethane led ... More
Synthesis and application of caged peptides and proteins.
AuthorsShigeri Y, Tatsu Y, Yumoto N,
JournalPharmacol Ther
PubMed ID11728602
Caged compounds have covalently attached groups that are rapidly cleaved upon exposure to UV light. Attachment of photolabile groups makes the molecule inert until photolysis releases it in its bioactive form. When caged compounds are applied to the experimental system in advance, the concentration jump of biologically active substances can ... More
Targeting expression with light using caged DNA.
AuthorsMonroe WT, McQuain MM, Chang MS, Alexander JS, Haselton FR
JournalJ Biol Chem
PubMed ID10409633
In this report, we describe the inactivation and site-specific light induction of plasmid expression using a photosensitive caging compound. Plasmids coding for luciferase were caged with 1-(4, 5-dimethoxy-2-nitrophenyl)diazoethane (DMNPE) and transfected into approximately 1-cm diameter sites of the skin of rats with particle bombardment. Skin sites transfected with caged plasmids ... More
Release of gelatinase A (matrix metalloproteinase 2) induced by photolysis of caged phosphatidic acid in HT 1080 metastatic fibrosarcoma cells.
AuthorsWilliger BT, Reich R, Neeman M, Bercovici T, Liscovitch M
JournalJ Biol Chem
PubMed ID8530350
Phosphatidic acid (PA) is a putative novel messenger in signal transduction and membrane traffic. We have synthesized a photolyzable derivative of PA, termed caged PA (cPA), which may be utilized as a new tool in studies of PA-mediated cellular events. 1-(2-Nitrophenyl)diazoethane, synthesized from 2-nitroacetophenone, was reacted with dipalmitoyl-PA to yield ... More