Effect of C1q on the processing of immune complexes by human neutrophils.
AuthorsOhkuro M, Kobayashi K, Takahashi K, Nagasawa S
JournalImmunology
PubMed ID7835978
'We prepared immune complexes (IC) composed of human anti-tetanus toxoid IgG and tetanus toxoid, and examined the effect of C1q on the processing of IC by human neutrophils. Treating IC with increasing amounts of C1q enhanced the binding and phagocytosis of IC by neutrophils, unless the amounts of C1q added ... More
Initial cytoplasmic and phagosomal consequences of human neutrophil exposure to Staphylococcus epidermidis.
AuthorsBernardo J, Long HJ, Simons ER,
JournalCytometry A
PubMed ID19937952
'Microorganisms are recognized by specific phagocyte surface receptors. Liganded receptors then signal a series of events leading to phagocytosis and destruction of the organism by oxidative, lytic, and associated processes. Some organisms, such as Mycobacterium tuberculosis (Mtb), Cryptococcus neoformans (Cf), and others, evade such destruction, surviving and sometimes multiplying within ... More
Stable accumulation of p67phox at the phagosomal membrane and ROS production within the phagosome.
AuthorsTlili A, Erard M, Faure MC, Baudin X, Piolot T, Dupré-Crochet S, Nüsse O,
JournalJ Leukoc Biol
PubMed ID21954286
'Production of ROS by the leukocyte NADPH oxidase is essential for the destruction of pathogenic bacteria inside phagosomes. The enzyme is a complex of cytosolic and membranous subunits that need to assemble upon activation. Biochemical data suggest that the complex is renewed continuously during activity. Furthermore, it is generally assumed ... More
Reactive oxygen species-producing site in radiation and hydrogen peroxide-induced apoptosis of human peripheral T cells: Involvement of lysosomal membrane destabilization.
AuthorsOgawa Y, Kobayashi T, Nishioka A, Kariya S, Ohnishi T, Hamasato S, Seguchi H, Yoshida S
JournalInt J Mol Med
PubMed ID15067365
'In our previous studies, we showed that the apoptotic resistance of the human osteosarcoma cell line HS-Os-1 against irradiation was easily converted to a state of apoptotic-susceptibility by the addition of a relatively low concentration of hydrogen peroxide to the culture medium just prior to irradiation. When we consider the ... More
Malaria inhibits surface expression of complement receptor 1 in monocytes/macrophages, causing decreased immune complex internalization.
AuthorsFernandez-Arias C, Lopez JP, Hernandez-Perez JN, Bautista-Ojeda MD, Branch O, Rodriguez A,
JournalJ Immunol
PubMed ID23440418
Complement receptor 1 (CR1) expressed on the surface of phagocytic cells binds complement-bound immune complexes (IC), playing an important role in the clearance of circulating IC. This receptor is critical to prevent accumulation of IC, which can contribute to inflammatory pathology. Accumulation of circulating IC is frequently observed during malaria, ... More
SHIP-1 increases early oxidative burst and regulates phagosome maturation in macrophages.
AuthorsKamen LA, Levinsohn J, Cadwallader A, Tridandapani S, Swanson JA,
JournalJ Immunol
PubMed ID18490750
Although the inositol phosphatase SHIP-1 is generally thought to inhibit signaling for Fc receptor-mediated phagocytosis, the product of its activity, phosphatidylinositol 3,4 bisphosphate (PI(3,4)P(2)), has been implicated in activation of the NADPH oxidase. This suggests that SHIP-1 positively regulates the generation of reactive oxygen species after phagocytosis. To examine how ... More
Prolonged production of reactive oxygen species in response to B cell receptor stimulation promotes B cell activation and proliferation.
AuthorsWheeler ML, Defranco AL,
JournalJ Immunol
PubMed ID23024271
We have investigated the intracellular sources and physiological function of reactive oxygen species (ROS) produced in primary B cells in response to BCR stimulation. BCR stimulation of primary resting murine B cells induced the rapid production of ROS that occurred within minutes and was maintained for at least 24 h ... More
Flow cytometric investigation of neutrophil oxidative burst and apoptosis in physiological and pathological situations.
AuthorsElbim C, Lizard G,
JournalCytometry A
PubMed ID19358285
Flow cytometric analysis provides a rapid screen for abnormalities of polymorphonuclear neutrophils (PMN) function and reflect their behavior in vivo more accurately. This review summarizes the major fluorescent probes used to study PMN oxidative burst and apoptosis using flow cytometry (FCM). We also provide examples of FCM studies in physiological ... More
Reactive oxygen species-producing site in radiation-induced apoptosis of human peripheral T cells: involvement of lysosomal membrane destabilization.
AuthorsOgawa Y, Kobayashi T, Nishioka A, Kariya S, Ohnishi T, Hamasato S, Seguchi H, Yoshida S
JournalInt J Mol Med
PubMed ID14654973
In our previous studies, we have partly elucidated the mechanism of radiation-induced apoptosis of human peripheral T cells. The exact site of the ROS (reactive oxygen species) formation induced by irradiation has been so far unknown. Therefore, in this study, we investigated the site of ROS formation by utilizing MitoCapture, ... More
Measurement of superoxide release in the phagovacuoles of immune complex-stimulated human neutrophils.
AuthorsRyan TC, Weil GJ, Newburger PE, Haugland R, Simons ER
JournalJ Immunol Methods
PubMed ID2165099
Immune complex stimulation of human neutrophils elicits, among other events, the formation of phagocytic vacuoles into which the products of the stimulus activated oxidative burst and degranulation are released. In order to monitor burst activity in the phagocytic vacuole, we have developed a fluorochrome-coupled derivative of this neutrophil agonist. The ... More
Evaluation of oxidative processes in human pigment epithelial cells associated with retinal outer segment phagocytosis.
AuthorsMiceli MV, Liles MR, Newsome DA
JournalExp Cell Res
PubMed ID8082727
To investigate the nature of the oxidative event that occurs during phagocytosis of retinal outer segments (ROS) by cultured human retinal pigment epithelial (RPE) cells, cells were incubated with isolated bovine ROS labeled with either the fluorescence probe carboxy-SNAFL-2 or the nonfluorescent, oxidizable probe 2',7'-dichlorodihydrofluorescein (H2DCF). The increase in fluorescence ... More
Reactive oxygen species-producing site in hydrogen peroxide-induced apoptosis of human peripheral T cells: involvement of lysosomal membrane destabilization.
AuthorsOgawa Y, Kobayashi T, Nishioka A, Kariya S, Ohnishi T, Hamasato S, Seguchi H, Yoshida S
JournalInt J Mol Med
PubMed ID14767567
In our previous study, we examined the effect of exogenous hydrogen peroxide, which causes a potent oxidative stress and has been demonstrated to be a potent apoptosis-inducer in many kinds of cells. We found that the addition of 1 or 10 mM hydrogen peroxide induced reactive oxygen species (ROS) formation, ... More
Kupffer cell-independent acute hepatocellular oxidative stress and decreased bile formation in post-cold-ischemic rat liver.
AuthorsKumamoto Y, Suematsu M, Shimazu M, Kato Y, Sano T, Makino N, Hirano KI, Naito M, Wakabayashi G, Ishimura Y, Kitajima M
JournalHepatology
PubMed ID10573525
The purpose of this study was to examine distribution and time history of oxidative stress during the hyperacute period of reperfusion in the liver grafts undergoing cold ischemia and to investigate roles of Kupffer cells as a potential oxidant source. Rat livers were harvested at 4 degrees C in University ... More
ROS-induced mitochondrial depolarization initiates PARK2/PARKIN-dependent mitochondrial degradation by autophagy.
Authors
JournalAutophagy
PubMed ID22889933
The receptor DNGR-1 signals for phagosomal rupture to promote cross-presentation of dead-cell-associated antigens.
Authors
JournalNat Immunol
PubMed ID33349708
Sub-ohm vaping increases the levels of carbonyls, is cytotoxic, and alters gene expression in human bronchial epithelial cells exposed at the air-liquid interface.
Authors
JournalRespir Res
PubMed ID33213456
Measurement of phagocytosis and of the phagosomal environment in polymorphonuclear phagocytes by flow cytometry.
Authors
JournalCurr Protoc Cytom
PubMed ID20069529
NOX2 oxidase expressed in endosomes promotes cell proliferation and prostate tumour development.
Authors
JournalOncotarget
PubMed ID30459931
Cell-specific toxicity of short-term JUUL aerosol exposure to human bronchial epithelial cells and murine macrophages exposed at the air-liquid interface.