Quantitation of corneal endothelial potentials using a carbocyanine dye.
AuthorsGraves C, Sachs G
JournalBiochim Biophys Acta
PubMed ID6977376
The carbocyanine dye, diS-C3-(5) was used to quantitate the plasma membrane potential of the bullfrog corneal endothelium. It was shown that valinomycin hyperpolarized the endothelial cell and that in the presence of the ionophore the membrane potential largely reflected the K+ equilibrium potential. Using calibration curves constructed by changing medium ... More
Visualization of the intracellular behavior of HIV in living cells.
'To track the behavior of human immunodeficiency virus (HIV)-1 in the cytoplasm of infected cells, we have tagged virions by incorporation of HIV Vpr fused to the GFP. Observation of the GFP-labeled particles in living cells revealed that they moved in curvilinear paths in the cytoplasm and accumulated in the ... More
Measurement of membrane potential in Bacillus subtilis: a comparison of lipophilic cations, rubidium ion, and a cyanine dye as probes.
AuthorsZaritsky A, Kihara M, Macnab RM
JournalJ Membr Biol
PubMed ID6796695
Monitoring the effect of an anti-cancer drug on RPMI 4788 cells by a membrane potential probe, dis-C3-(5).
AuthorsIwagaki H, Fuchimoto S, Shiiki S, Miyake M, Orita K
JournalJ Med
PubMed ID2504864
Use of intracellular calcium and membrane potential fluorescent indicators in neuroendocrine cells.
AuthorsCapponi AM, Lew PD, Schlegel W, Pozzan T
JournalMethods Enzymol
PubMed ID3713520
Prostaglandin E2 stimulates a Ca2+-dependent K+ channel in human erythrocytes and alters cell volume and filterability.
AuthorsLi Q, Jungmann V, Kiyatkin A, Low PS
JournalJ Biol Chem
PubMed ID8702518
'To understand the mechanism by which human red blood cells (RBCs) contribute to hemostasis and thrombosis, we have examined the effects of metabolites released by activated platelets on intact RBCs. Prostaglandin E2 (PGE2), a signal molecule produced by activated platelets, was observed to lower the filterability of human erythrocytes by ... More
Electrophysiology of plasma membrane vesicles.
AuthorsWright EM
JournalAm J Physiol
PubMed ID6372509
'In both renal and gastrointestinal physiology, it has become popular to study epithelial transport phenomena using vesicles isolated from the apical and basolateral cell membranes. Transport in vesicle preparations is usually monitored with radioactive tracers, but more recently attention has been directed to electrophysiological methods. As it is impossible to ... More
A pore-forming toxin interacts with a GPI-anchored protein and causes vacuolation of the endoplasmic reticulum.
AuthorsAbrami L, Fivaz M, Glauser PE, Parton RG, van der Goot FG
JournalJ Cell Biol
PubMed ID9456314
'In this paper, we have investigated the effects of the pore-forming toxin aerolysin, produced by Aeromonas hydrophila, on mammalian cells. Our data indicate that the protoxin binds to an 80-kD glycosyl-phosphatidylinositol (GPI)-anchored protein on BHK cells, and that the bound toxin is associated with specialized plasma membrane domains, described as ... More
Time dependence of transmembrane potential changes and intracellular calcium flux in stimulated human monocytes.
AuthorsBernardo J, Brink HF, Simons ER
JournalJ Cell Physiol
PubMed ID2826501
'An important characteristic of the functional differentiation of the blood monocyte is the development of its capacity to recognize and respond to stimuli. This ability is mediated to a large extent by specific receptor glycoproteins located on the cell surface. Stimulation of mononuclear phagocytes via these receptors results in a ... More
Selective modulation by cGMP of the K+ channel activated by speract.
AuthorsCook SP, Babcock DF
JournalJ Biol Chem
PubMed ID7693667
'The egg peptide speract stimulates sperm guanylyl cyclase and presumably enhances fertilization, but the roles of cGMP in sperm responses are yet undetermined. Here we show that speract-induced accumulation of cGMP or cAMP is selectively enhanced by the phosphodiesterase inhibitors, 3-isobutyl-1-methylxanthine (IBMX) or papaverine, respectively. These inhibitors provided the unusual ... More
Transmembrane potentials in cells: a diS-C3(3) assay for relative potentials as an indicator of real changes.
AuthorsPlásek J, Dale RE, Sigler K, Laskay G
JournalBiochim Biophys Acta
PubMed ID7841182
'The mechanism by which the fluorescent cationic dye diS-C3(3) reports on cellular transmembrane potential has been investigated in murine haemopoietic cells. Due to the large molar absorbance of diS-C3(3) and its high quantum yield of fluorescence in cells, this dye can be used at very low labelling concentrations (5 x ... More
Dihydropyridine-sensitive initial component of the ANG II-induced Ca2+ response in rat adrenal glomerulosa cells.
AuthorsHunyady L, Rohács T, Bagó A, Deák F, Spät A
JournalAm J Physiol
PubMed ID7508191
'The Ca2+ signal induced by an increase in extracellular K+ concentration from 3.6 to 5.6 mM or angiotensin II (ANG II) was inhibited by the dihydropyridine (DHP) Ca2+ channel blocker, nifedipine, and enhanced by the DHP Ca2+ channel agonist, BAY K 8644. The DHP sensitivity of the ANG II-induced Ca2+ ... More
Mitochondrial membrane potential in lymphocytes as monitored by fluorescent cation diS-C3-(5).
AuthorsGulyaeva NV, Konoshenko GI, Mokhova EN
JournalMembr Biochem
PubMed ID4033446
'A lipophilic fluorescent cation diS-C3-(5) and rotenone suppress the oxygen consumption rate of thymocytes in similar concentrations. Seventy percent inhibition corresponds to an inhibitor:cytochrome a molar ratio of about 1:1. Addition of uncouplers decreases the inhibition of respiration by diS-C3-(5) (but not rotenone). FCCP in similar concentrations increases O2 consumption ... More
'Adenylylcyclase (AC) from sea urchin sperm does not appear to be regulated by G proteins [Hildebrandt, J. D., Tash, J. S., Kirchick, H. J., Lipschunits, L., Secra, R. D., & Birmbaumer, L. (1985) Endocrinology 116, 1357-1366]. During sperm activation and the acrosome reaction, membrane potential changes and cAMP increases. Here ... More
Effect of bacterial endotoxin on the transmembrane electrical potential and plasma membrane fluidity of human monocytes.
AuthorsLarsen NE, Enelow RI, Simons ER, Sullivan R
JournalBiochim Biophys Acta
PubMed ID3986196
'In order to gain insight into the physical interaction between bacterial endotoxins and the surface of human monocytes, we investigated the effects of Salmonella typhi endotoxin and lipid A on two functional properties of the plasma membrane of these cells: (1) the transmembrane electrical potential and (2) the fluidity of ... More
Chemotactic factor-induced membrane potential changes in rabbit neutrophils monitored by the fluorescent dye 3,3'-dipropylthiadicarbocyanine iodide.
AuthorsTatham PE, Delves PJ, Shen L, Roitt IM
JournalBiochim Biophys Acta
PubMed ID7426651
'Rabbit neutrophil leucocytes take up the cationic, fluorescent dye 3,3''-dipropylthiadicarbocyanine iodide (DiS-C3-(5)). Treatment with valinomycin and K+ then produces characteristic changes in suspension fluorescence that indicate that the dye enters the cells in a potential-dependent fashion and that the resting membrane potential lies between -66 and -86 mV. The peptide, ... More
Cyanine dye fluorescence used to measure membrane potential changes due to the assembly of complement proteins C5b-9.
AuthorsWiedmer T, Sims PJ
JournalJ Membr Biol
PubMed ID4032456
'The fluorescent potentiometric indicator diS-C3-(5) has been used to investigate changes in membrane potential due to assembly of the C5b-9 membrane attack complex of the complement system. EAC1-7 human red blood cells and resealed erythrocyte ghosts--bearing membrane-assembled C5b67 complexes--were generated by immune activation in C8-deficient human serum. Studies performed with ... More
Potential-sensitive response mechanism of diS-C3-(5) in biological membranes.
AuthorsCabrini G, Verkman AS
JournalJ Membr Biol
PubMed ID3761361
'The potential-sensitive response mechanism of 3,3'-dipropylthiodicarbocyanine iodide (diS-C3-(5] was examined based on our previous model of diS-C3-(5) interaction with brush border membrane vesicles (BBMV) in the absence of a membrane potential. The model contained binding (6 msec), reorientation (30 msec), dimerization (less than 10 nsec), and translocation (1 sec) reaction ... More
Changes in cytoplasmic pH and in membrane potential in thrombin-stimulated human platelets.
AuthorsHorne WC, Norman NE, Schwartz DB, Simons ER
JournalEur J Biochem
PubMed ID7318826
'The response of human platelets to stimulation by a specific aggregant such as thrombin has been postulated to proceed sequentially via induction of response at the membrane, followed by execution of shape change, secretion, and aggregation of the platelets. We have shown earlier that the platelet response includes a depolarization ... More
Enhanced bactericidal action of lysozyme to Escherichia coli by inserting a hydrophobic pentapeptide into its C terminus.
AuthorsIbrahim HR, Yamada M, Matsushita K, Kobayashi K, Kato A
JournalJ Biol Chem
PubMed ID8106483
'The mechanism of the enhanced bactericidal action to Escherichia coli of the lysozyme having a hydrophobic pentapeptide (Phe-Phe-Val-Ala-Pro) at its C terminus was investigated. The modified lysozyme, hydrophobic pentapeptide-fused lysozyme (HLz), was secreted in the culture medium from yeast harboring the expression plasmid, in which a synthetic DNA fragment encoding ... More
Mitochondrial membrane potential is dependent on the oligomeric state of F1F0-ATP synthase supracomplexes.
AuthorsBornhövd C, Vogel F, Neupert W, Reichert AS
JournalJ Biol Chem
PubMed ID16551625
'The F1F0-ATP synthase in mitochondria, in addition to its function in energy transduction, has a structural role in determining cristae morphology. This depends on its ability to form dimeric and higher oligomeric supracomplexes. Here we show that mutants of the dimer-specific subunits e and g, which destabilize dimeric and oligomeric ... More
The effects of mitochondrial energetics inhibitors on the fluorescence of potential-sensitive dyes rhodamine 123 and diS-C3-(5) in lymphocyte suspensions.
AuthorsMokhova EN, Rozovskaya IA
JournalJ Bioenerg Biomembr
PubMed ID3745149
'The effects of uncouplers (FCCP, DNF), oligomycin, and rotenone on the fluorescence of potential-sensitive dyes, rhodamine 123 and diS-C3-(5), in lymphocyte suspensions were compared. The fluorescence of these optical probes gradually increased at higher FCCP concentrations. The dependences of fluorescence intensities and FCCP concentrations were similar for both dyes, and ... More
Hemisodium, a novel selective Na ionophore. Effect on normal human erythrocytes.
AuthorsKaji DM
JournalJ Gen Physiol
PubMed ID1613483
'Hemisodium is a novel Na ionophore that belongs to the class of compounds called cryptands. These compounds possess an electron-rich cavity for binding of cations and are conformationally organized during synthesis to favor the selective binding of one cation over another. In media containing 145 mM NaCl and 5 mM ... More
Effect of lipid composition changes on carbocyanine dye fluorescent response.
AuthorsDeleers M, Servais JP, de Laveleye F, Wulfert E
JournalBiochem Biophys Res Commun
PubMed ID6541041
'Egg yolk phosphatidyl choline liposomes containing variable amounts of phosphatidyl ethanolamine, phosphatidyl inositol or phosphatidyl serine demonstrated important variations in the fluorescence of 3.3'' dipropylthiodicarbocyanine. When the membrane contained no cholesterol, fluorescence was not correlated with membrane fluidity as measured by diphenyl hexatriene polarization. Increasing cholesterol concentration in valinomycin containing ... More
Effects of staphylococcin 1580 on cells and membrane vesicles of Bacillus subtilis W23.
AuthorsWeerkamp A, Vogels GD
JournalBiochim Biophys Acta
PubMed ID415763
'1. Uptake of L-glutamic acid is inhibited, and preaccumulated L-glutamic acid is released from Bacillus subtilis cells treated with staphylococcin 1580. Uptake of alpha-methylglycoside is enhanced at low bacteriocin concentrations and inhibited by excess bacteriocin. 2. Inhibition of amino acid uptake into membrane vesicles is somewhat less sensitive to staphylococcin ... More
Triggering of lymphocyte capping appears not to require changes in potential or ion fluxes across the plasma membrane.
'Capping induced by anti-Ig antibody on mouse spleen lymphocytes was found to proceed normally over a wide range of membrane potentials from approx. 0 to -65 mV, as estimated with fluorescent probes. The potential was manipulated by ionic substitution in the medium and/or application of gramicidin. Various agents which inhibit ... More
Temperature dependence of membrane ion conductance analyzed by using the amphiphilic anion 5/6-carboxyfluorescein.
AuthorsBramhall J, Hofmann J, DeGuzman R, Montestruque S, Schell R
JournalBiochemistry
PubMed ID3427008
'The rate of efflux of trapped 5/6-carboxyfluorescein from sealed lipid vesicles showed a marked dependence on (a) temperature, (b) phospholipid acyl chain composition, and (c) the nature of co-trapped counterions. When the dye was salted with sodium, at pH greater than 7, the rate of dye permeation showed a discrete ... More
Simultaneous determination of membrane potential and pH gradient by photodiode array spectroscopy.
AuthorsKonishi T, Murakami N, Hatano Y, Nakazato K
JournalBiochim Biophys Acta
PubMed ID3778892
'Membrane potential (delta psi) and pH difference (delta pH) were simultaneously determined in liposomes using a photodiode array spectrophotometer. By the use of a cyanine dye (DiS-C3(5)) and 9-aminoacridine for delta psi and delta pH probes, respectively, both changes of delta psi and delta pH could be successfully determined by ... More
Correlation between cytosolic free Ca2+ and aldosterone production in bovine adrenal glomerulosa cells. Evidence for a difference in the mode of action of angiotensin II and potassium.
AuthorsCapponi AM, Lew PD, Jornot L, Vallotton MB
JournalJ Biol Chem
PubMed ID6746627
'Quantitative changes in cytosolic free calcium [( Ca2+]i), membrane potential, and aldosterone production in response to angiotensin II and extracellular potassium were measured in intact bovine adrenal glomerulosa cells loaded with the fluorescent calcium indicator quin 2. Angiotensin II (10(-9) M) induced a rapid rise in [Ca2+]i from 124 +/- ... More
Effects of dihydropyridines and inorganic calcium blockers on aggregation and on intracellular free calcium in platelets.
AuthorsPalés J, Palacios-Araus L, López A, Gual A
JournalBiochim Biophys Acta
PubMed ID1645196
'[Ca2+]i increase is necessary in physiological platelet activity, particularly aggregation and release. The increase of [Ca2+]i observed during platelet activation depends in part on Ca2+ influx from the extracellular medium. The participation of voltage-operated Ca2+ channels as a pathway for Ca2+ entry is controversial. In the present study we have ... More
Anomalous driving force for renal brush border H+/OH-transport characterized by using 6-carboxyfluorescein.
AuthorsVerkman AS, Ives HE
JournalBiochemistry
PubMed ID3013286
'The pH, delta pH, and membrane potential dependences of H+/OH-permeability in renal brush border membrane vesicles (BBMV) were studied by using the entrapped pH indicator 6-carboxyfluorescein (6CF). Quantitative H+/OH-fluxes (JH) were obtained from a calibration of the fluorescence response of 6CF to intravesicular pH using vesicles prepared with varying intravesicular ... More
Ion permeability of rabbit intestinal brush border membrane vesicles.
AuthorsGunther RD, Schell RE, Wright EM
JournalJ Membr Biol
PubMed ID6716451
'The ion permeability of rabbit jejunal brush border membrane vesicles was studied by measuring unidirectional fluxes with radioactive tracers and bi-ionic diffusion potentials with the potential-sensitive fluorescent dye, diS-C3-(5). Tracer measurements provide estimates of the absolute magnitudes of permeability coefficients, while fluorescence measurements provide estimates of relative and absolute ion ... More
Interaction of the extrinsic potential-sensitive molecular probe diS-C3-(5) with pigeon heart mitochondria under equilibrium and time-resolved conditions.
AuthorsBammel BP, Brand JA, Germon W, Smith JC
JournalArch Biochem Biophys
PubMed ID3004342
'Some aspects of the interaction of the extrinsic, potential-sensitive, molecular probe diS-C3-(5) with pigeon heart mitochondria are reported in this paper. Binding studies based on fluorimetry indicate that the ratio of the dissociation constant to the maximum number of binding sites, KD/n, is larger for succinate-containing mitochondria than that for ... More
Absence of cardiolipin in the crd1 null mutant results in decreased mitochondrial membrane potential and reduced mitochondrial function.
AuthorsJiang F, Ryan MT, Schlame M, Zhao M, Gu Z, Klingenberg M, Pfanner N, Greenberg ML
JournalJ Biol Chem
PubMed ID10777514
'Cardiolipin (CL) is a unique phospholipid which is present throughout the eukaryotic kingdom and is localized in mitochondrial membranes. Saccharomyces cerevisiae cells containing a disruption of CRD1, the structural gene encoding CL synthase, have no CL in mitochondrial membranes. To elucidate the physiological role of CL, we compared mitochondrial functions ... More
Cytoplasmic Ca2+ is necessary for thrombin-induced platelet activation.
AuthorsDavies TA, Drotts DL, Weil GJ, Simons ER
JournalJ Biol Chem
PubMed ID2511190
'alpha-Thrombin induces a dose-dependent rapid transient increase in platelet cytosolic Ca2+ levels, coming solely from intracellular stores, since EGTA has no effect. In contrast, the post-stimulation equilibrium [Ca2+]in depends upon an influx from the extracellular milieu, and is lower in the presence of EGTA. We measured the Ca2+ transient (with ... More
A noninvasive fluorimetric procedure for measurement of membrane potential. Quantification of the NADPH oxidase-induced depolarization in activated neutrophils.
AuthorsJankowski A, Grinstein S
JournalJ Biol Chem
PubMed ID10473559
'The electrogenic activity of the NADPH oxidase is associated with depolarization of the plasma membrane in activated neutrophils. The magnitude and consequences of this depolarization, however, remain unknown. Neutrophils are not amenable to electrophysiological determinations of membrane potential by current clamp. Instead, the occurrence of depolarization has been inferred from ... More
The lysosomal proton pump is electrogenic.
AuthorsHarikumar P, Reeves JP
JournalJ Biol Chem
PubMed ID6224789
'Lysosomes were purified approximately 40-fold from rat kidney cortex by differential and Percoll density gradient centrifugation. In a sucrose medium, the lysosomes quenched the fluorescence of the potential sensitive dye diS-C3-(5) (3,3''-dipropylthiocarbo-cyanine iodide) in a time-dependent manner, indicating that the dye accumulates within the lysosomal interior. After treatment of the ... More
Internal pH can regulate Ca2+ uptake and the acrosome reaction in sea urchin sperm.
AuthorsGarcía-Soto J, González-Martínez M, de De la Torre L, Darszon A
JournalDev Biol
PubMed ID3817283
'The egg jelly-induced acrosome reaction of sea urchin sperm is accompanied by intracellular alkalinization and Ca2+ entry. We have previously shown that in the absence of egg jelly, NH4Cl, which increases intracellular pH (pHi), induces Ca2+ uptake and the acrosome reaction in sperm of the sea urchin, Strongylocentrotus purpuratus. Here ... More
Replacement of glycine 232 by aspartic acid in the KdpA subunit broadens the ion specificity of the K(+)-translocating KdpFABC complex.
AuthorsSchrader M, Fendler K, Bamberg E, Gassel M, Epstein W, Altendorf K, Dröse S
JournalBiophys J
PubMed ID10920013
'Replacement of glycine residue 232 with aspartate in the KdpA subunit of the K(+)-translocating KdpFABC complex of Escherichia coli leads to a transport complex that has reduced affinity for K(+) and has lost the ability to discriminate Rb(+) ions (, J. Biol. Chem. 270:6678-6685). This glycine residue is the first ... More
Regulation of the plasma membrane Ca2+ pump by cyclic nucleotides in cultured vascular smooth muscle cells.
AuthorsFurukawa K, Tawada Y, Shigekawa M
JournalJ Biol Chem
PubMed ID2836409
'We investigated the mechanisms of Ca2+ extrusion from cultured rat aortic smooth muscle cells while monitoring changes in the cytosolic Ca2+ concentration ([Ca2+]i) using fura 2 fluorescence. 45Ca2+ efflux from these cells consisted of two major mechanisms; one was dependent on the extracellular sodium concentration (Na+o) and the other was ... More
Telavancin, a multifunctional lipoglycopeptide, disrupts both cell wall synthesis and cell membrane integrity in methicillin-resistant Staphylococcus aureus.
AuthorsHiggins DL, Chang R, Debabov DV, Leung J, Wu T, Krause KM, Sandvik E, Hubbard JM, Kaniga K, Schmidt DE, Gao Q, Cass RT, Karr DE, Benton BM, Humphrey PP
JournalAntimicrob Agents Chemother
PubMed ID15728913
'The emergence and spread of multidrug-resistant gram-positive bacteria represent a serious clinical problem. Telavancin is a novel lipoglycopeptide antibiotic that possesses rapid in vitro bactericidal activity against a broad spectrum of clinically relevant gram-positive pathogens. Here we demonstrate that telavancin''s antibacterial activity derives from at least two mechanisms. As observed ... More
A high-throughput assay for mitochondrial membrane potential in permeabilized yeast cells.
AuthorsFarrelly E, Amaral MC, Marshall L, Huang SG
JournalAnal Biochem
PubMed ID11399043
'A fluorometric assay for mitochondrial membrane potential in permeabilized yeast cells has been developed. This method involves permeabilizing the plasma membrane and measuring the distribution of a mitochondrial membrane potential sensitive probe 3,3''-dipropylthiadicarbocyanine iodide (DiSC(3)(5); DiSC(3)). In permeabilized cells, DiSC(3) fluorescence decreased when introduced into energized mitochondria and increased three- ... More
Sperm membrane potential: hyperpolarization during capacitation regulates zona pellucida-dependent acrosomal secretion.
AuthorsZeng Y, Clark EN, Florman HM
JournalDev Biol
PubMed ID7556936
'Membrane potential (VM) was investigated in mouse and bovine sperm populations. VM was determined from the fluorescence emission of the lipophilic anion, bis(1,3-diethylthiobarbituric acid)trimethine oxonol (DiSBAC2(3)), and from the lipophilic cation, 3,3''-dipropylthiodicarbocyanine iodide (DiSC3(5)). Fluorescent signals were corrected for contributions of mitochondrial potentials and apparent VM values were obtained by ... More
Presynaptic GABA(B) receptor modulation of glutamate exocytosis from rat cerebrocortical nerve terminals: receptor decoupling by protein kinase C.
AuthorsPerkinton MS, Sihra TS
JournalJ Neurochem
PubMed ID9523568
'GABA and the GABA(B) receptor agonist (-)-baclofen inhibited 4-aminopyridine (4AP)- and KCl-evoked, Ca2+-dependent glutamate release from rat cerebrocortical synaptosomes. The GABA(B) receptor antagonist CGP 35348, prevented this inhibition of glutamate release, but phaclofen had no effect. (-)-Baclofen-mediated inhibition of glutamate release was insensitive to 2 microg/ml pertussis toxin. As determined ... More
Mechanism of polymorphonuclear leukocyte activation by myristate. Involvement of calcium ion and protein kinase C.
AuthorsYamamoto M, Okimasu E, Terada S, Utsumi K
JournalCell Struct Funct
PubMed ID3040274
'The stimulative effects of myristate on the superoxide generation and depolarization of membrane potential of polymorphonuclear leukocytes (PMN) are particularly strong, yet myristate does not affect the intracellular free Ca2+ level ([Ca2+]i) in the presence of 1 microM free calcium in calcium-EGTA buffer. The half maximum concentration of myristate was ... More
Kinetics of sodium-dependent solute transport by rabbit renal and jejunal brush-border vesicles using a fluorescent dye.
AuthorsSchell RE, Stevens BR, Wright EM
JournalJ Physiol
PubMed ID6875880
'The kinetics of Na-coupled solute transport by renal and jejunal brush-border vesicles in the rabbit were examined using the potential-sensitive fluorescent dye diS-C3-(5). All organic solutes known to be transported across these membranes by Na-coupled mechanisms increase the fluorescence of the dye in the presence of Na, but not K. ... More
Membrane potentials in respiring and respiration-deficient yeasts monitored by a fluorescent dye.
AuthorsKovác L, Varecka L
JournalBiochim Biophys Acta
PubMed ID7028114
'Changes in fluorescence of 3,3''-dipropylthiodicarbocyanine iodide which had been equilibrated with suspensions of the wild-type yeast Saccharomyces cerevisiae and of respiration-deficient mutants were followed. The changes have been attributed to changes of yeast membrane potentials, since the fluorescence with wild-type yeast could be affected in a predictable manner by uncouplers ... More
Release of a fluorescent probe as an indicator of lysosomal granule secretion by thrombin-stimulated human platelets.
AuthorsGreenberg-Sepersky SM, Simons ER
JournalAnal Biochem
PubMed ID4025824
'Investigations in this laboratory have demonstrated that thrombin induces dose-dependent changes in the transmembrane electrical potential of gel-filtered human platelets. This change is monitored with the fluorescent lipophilic cation, 3,3''-dipropylthiodicarbocyanine (diS-C3-(5], whose rapid release from the platelet (maximal within 30 s) correlates with a rapid, dose-dependent influx of sodium, a ... More
The putative electrogenic nitrate-proton symport of the yeast Candida utilis. Comparison with the systems absorbing glucose or lactate.
AuthorsEddy AA, Hopkins PG
JournalBiochem J
PubMed ID2998345
'Strain N.C.Y.C. 193 of Candida utilis was grown aerobically at 30 degrees C with nitrate as limiting nutrient in a chemostat. The washed yeast cells depleted of ATP absorbed up to 5 nmol of nitrate/mg dry wt. of yeast. At pH 4-6, extra protons and nitrate entered the yeast cells ... More
Electrochemical potential and ion transport in vesicles of yeast plasma membrane.
AuthorsCalahorra M, Ramírez J, Clemente SM, Peña A
JournalBiochim Biophys Acta
PubMed ID2883994
'Vesicles from yeast plasma membrane were prepared according to Franzusoff and Cirillo [1983) J. Biol. Chem. 258, 3608), with slight modifications. When Mg-ATP was added, this preparation was able to generate a membrane potential, that was sensitive to inhibitors of the yeast H+-ATPase and uncouplers, and could be decreased by ... More
Peptide transport in rabbit intestinal brush-border membrane vesicles studied with a potential-sensitive dye.
AuthorsGanapathy V, Burckhardt G, Leibach FH
JournalBiochim Biophys Acta
PubMed ID3839134
'Peptide transport in purified rabbit intestinal brush-border membrane vesicles has been studied using a potential-sensitive fluorescent dye, di-S-C3(5). Transport of dipeptides is accompanied by an increase in the fluorescence of the dye in the presence and absence of Na+, indicating electrogenic, Na+-independent peptide transport. Dipeptides containing D-amino acids also increase ... More
Differential roles of Fc gamma RII and Fc gamma RIII in immune complex stimulation of human neutrophils.
AuthorsBrunkhorst BA, Strohmeier G, Lazzari K, Weil G, Melnick D, Fleit HB, Simons ER
JournalJ Biol Chem
PubMed ID1328206
'Insoluble immune complexes (IIC) stimulate human neutrophils through Fc gamma receptors. Freshly isolated human neutrophils express two FcR subclasses, FcRII and FcRIII. We explored the role of FcRII and FcRIII in this activation process by selectively binding each FcR subclass with the Fab fragments of the respective anti-FcR monoclonal antibodies ... More
Thallium mediates a rapid chloride/hydroxyl ion exchange through myelin lipid bilayers.
AuthorsDíaz RS, Monreal J
JournalMol Pharmacol
PubMed ID7808444
'We have investigated the effects of several heavy metal cations on the proton and chloride permeabilities of liposomes prepared with endogenous lipids from brain myelin, by monitoring the fluorescence emitted by acridine orange and N-(6-methoxyquinolyl)acetoethyl ester. In addition to Hg2+ and Cu+, nanomolar concentrations of Tl3+, but not Tl+, were ... More
Cytoplasmic [Ca2+] and intracellular pH in lymphocytes. Role of membrane potential and volume-activated Na+/H+ exchange.
AuthorsGrinstein S, Cohen S
JournalJ Gen Physiol
PubMed ID3559512
'The effect of elevating cytoplasmic Ca2+ [( Ca2+]i) on the intracellular pH (pHi) of thymic lymphocytes was investigated. In Na+-containing media, treatment of the cells with ionomycin, a divalent cation ionophore, induced a moderate cytoplasmic alkalinization. In the presence of amiloride or in Na+-free media, an acidification was observed. This ... More
Calcium-activated potassium channels in human platelets.
AuthorsFine BP, Hansen KA, Salcedo JR, Aviv A
JournalProc Soc Exp Biol Med
PubMed ID2510175
'The cationic fluorescent probe, DiSC3(5) was used to measure the membrane potential in human platelets. Hyperpolarization was induced by the addition of Ca2+ to the medium and also by the addition of the Ca2+ ionophore, A23187. In the absence of extracellular Ca2+ ([Ca2+]o) there was no response to A23187. The ... More
Voltage-sensitive calcium flux promoted by vesicles in an isolated cardiac sarcolemma preparation.
AuthorsSchilling WP, Lindenmayer GE
JournalJ Membr Biol
PubMed ID6431112
'The effect of membrane potential on the vesicular uptake of calcium in an isolated cardiac sarcolemma preparation from canine ventricle was evaluated. Membrane potentials were developed by the establishment of potassium gradients across the vesicular membranes. In the presence of valinomycin, the fluorescence changes of the voltage sensitive dye, diS-C3-(5) ... More
Acidification and ion permeabilities of highly purified rat liver endosomes.
AuthorsFuchs R, Mâle P, Mellman I
JournalJ Biol Chem
PubMed ID2914902
'While it is well established that acidic pH in endosomes plays a critical role in mediating the orderly traffic of receptors and ligands during endocytosis, little is known about the bioenergetics or regulation of endosome acidification. Using highly enriched fractions of rat liver endosomes prepared by free flow electrophoresis and ... More
Dicarbocyanine fluorescent probes of membrane potential block lymphocyte capping, deplete cellular ATP and inhibit respiration of isolated mitochondria.
AuthorsMontecucco C, Pozzan T, Rink T
JournalBiochim Biophys Acta
PubMed ID87222
'3,3''-Dipropylthiodicarbocyanine iodide, a widely used fluorescent probe of membrane potential, was found to inhibit anti-Ig antibody, induced capping of mouse lymphocytes. The dye also lowered the cell ATP content. Experiments with isolated mitochondria revealed that the probe had a potent inhibitory action at site I of the respiratory chain. This ... More
Evidence for direct involvement of the sarcoplasmic reticulum Ca(2+)-ATPase in a passive monovalent cation (K+/Na+) exchange.
Authorsde Jesus F, Cuillel M, Dupont Y
JournalFEBS Lett
PubMed ID7498534
'A specific inhibitor of SERCA-pumps, thapsigargin (TG) was used to demonstrate the direct involvement of the SR Ca(2+)-ATPase in passive K+/Na+ exchange. The K(+)-potential variations across vesicle membranes were measured in the absence of ATP with a fluorescent probe: 3,3''-dipropylthiodicarbocyanine iodide. Addition of EGTA dissipates the K(+)-potential whereas the presence ... More
High K+ elevates cytosolic free Ca concentration due to mobilization from internal storage sites in rat parotid cells.
AuthorsTakemura H, Ohshika H
JournalComp Biochem Physiol A
PubMed ID2452048
'1. Effects of high K+ on cytosolic free Ca concentration ([Ca2+]i) in rat parotid cells were studied using quin2. 2. High K+ elevated [Ca2+]i in a dose-dependent manner in normal and Ca-free media. The elevation of [Ca2+]i induced by high K+ was less in the latter medium. 3. High K+ ... More
Heliothis virescens and Manduca sexta lipid rafts are involved in Cry1A toxin binding to the midgut epithelium and subsequent pore formation.
AuthorsZhuang M, Oltean DI, Gómez I, Pullikuth AK, Soberón M, Bravo A, Gill SS
JournalJ Biol Chem
PubMed ID11836242
'Lipid rafts are characterized by their insolubility in nonionic detergents such as Triton X-100 at 4 degrees C. They have been studied in mammals, where they play critical roles in protein sorting and signal transduction. To understand the potential role of lipid rafts in lepidopteran insects, we isolated and analyzed ... More
Spectroscopic evidence for interactions of merocyanine 540 with valinomycin in the presence of potassium.
AuthorsLavie E, Sonenberg M
JournalFEBS Lett
PubMed ID7358170
Cell suspensions from porcine olfactory mucosa. Changes in membrane potential and membrane fluidity in response to various odorants.
AuthorsKashiwayanagi M, Sai K, Kurihara K
JournalJ Gen Physiol
PubMed ID3559517
'A suspension of olfactory epithelial cells was prepared from porcine olfactory mucosa and the physiological functions of the suspension were examined. The membrane potential of the cell suspension, which was monitored by measuring the fluorescence changes of rhodamine 6G, was depolarized by an increase in the K+ concentration in the ... More
Altering the speract-induced ion permeability changes that generate flagellar Ca2+ spikes regulates their kinetics and sea urchin sperm motility.
AuthorsWood CD, Nishigaki T, Tatsu Y, Yumoto N, Baba SA, Whitaker M, Darszon A
JournalDev Biol
PubMed ID17467684
'Speract, an egg-derived sperm-activating peptide, induces changes in intracellular Ca2+, Na+, pH, cAMP, cGMP, and membrane potential in sperm of the sea urchin Strongylocentrotus purpuratus. Ca2+ is a key regulator of motility in all sperm and, in many marine species, is required for generating turns interspersed with straighter swimming paths ... More
Ion diffusion potentials across mycoplasma membranes determined by a novel method using a carbocyanine dye.
AuthorsSchummer U, Schiefer HG
JournalArch Biochem Biophys
PubMed ID3947080
'The influence of transmembrane ion fluxes on mycoplasma membrane potentials was studied. Electric membrane potential was calibrated vs fluorescence intensity of a potential-sensitive carbocyanine dye according to delta psi = (RT/F) X log([aIN(1 - IN) - b]/Kint), where IN = I/I0, I0 = maximal fluorescence intensity (obtained for delta psi----infinity), ... More
Differentiation of Phytophthora infestans sporangia from other airborne biological particles by flow cytometry.
AuthorsDay JP, Kell DB, Griffith GW
JournalAppl Environ Microbiol
PubMed ID11772606
'The ability of two different flow cytometers, the Microcyte (Optoflow) and the PAS-III (Partec), to differentiate sporangia of the late-blight pathogen Phytophthora infestans from other potential airborne particles was compared. With the PAS-III, light scatter and intrinsic fluorescence parameters could be used to differentiate sporangia from conidia of Alternaria or ... More
The concentration-dependent membrane activity of cecropin A.
'Cecropin A is a naturally occurring, linear, cationic, 37-residue antimicrobial peptide. The precise mechanism by which it kills bacteria is not known, but its site of action is believed to be the cell membrane. To investigate the nature of its membrane activity, we examined the ability of cecropin A to ... More
Oxonol dyes as monitors of membrane potential: the effect of viruses and toxins on the plasma membrane potential of animal cells in monolayer culture and in suspension.
AuthorsBashford CL, Alder GM, Gray MA, Micklem KJ, Taylor CC, Turek PJ, Pasternak CA
JournalJ Cell Physiol
PubMed ID3988810
'Optical indicators of the cationic, cyanine and anionic oxonol classes were used to evaluate the plasma membrane potential of animal cells in suspension and in monolayer culture. The optical signals were calibrated by using diffusion potentials either of K+ (in the presence of valinomycin) or of H+ (in the presence ... More
Disparate effects of interferon-gamma and tumor necrosis factor-alpha on early neutrophil respiratory burst and fungicidal responses to Candida albicans hyphae in vitro.
AuthorsDiamond RD, Lyman CA, Wysong DR
JournalJ Clin Invest
PubMed ID1846880
'We examined effects of priming with recombinant human interferon-gamma (IFN) or tumor necrosis factor-alpha (TNF) on neutrophil responses to Candida albicans hyphae. Both cytokines increased early superoxide generation after hyphal stimulation. The more pronounced effects of TNF were accompanied by an augmented surface membrane depolarization rate and were insensitive to ... More
Mechanisms for the modulation of membrane bilayer properties by amphipathic helical peptides.
AuthorsEpand RM, Shai Y, Segrest JP, Anantharamaiah GM
JournalBiopolymers
PubMed ID7632881
'The amphipathic helix, in which hydrophobic and hydrophilic residues are grouped on opposing faces, is a structural motif found in many peptides and proteins that bind to membranes. One of the physical properties of membranes that can be altered by the binding of amphipathic helices is membrane monolayer curvature strain. ... More
The relation between dicarbocyanine dye fluorescence and the membrane potential of human red blood cells set at varying Donnan equilibria.
AuthorsFreedman JC, Hoffman JF
JournalJ Gen Physiol
PubMed ID39969
'The fluorescence, F, of two dicarbocyanine dyes, diS-C3(5) and diI-C3(5), depends both on the membrane potential, E, and on the intracellular pH, pHc, or human red blood cells. Compositions of isotonic media have been devised in which the equilibrium Donnan potential, E, varies at constant pHc and in which pHc ... More
Non-age related differences in thrombin responses by platelets from male patients with advanced Alzheimer's disease.
AuthorsDavies TA, Fine RE, Johnson RJ, Levesque CA, Rathbun WH, Seetoo KF, Smith SJ, Strohmeier G, Volicer L, Delva L
JournalBiochem Biophys Res Commun
PubMed ID8333868
'Alzheimer''s Disease(AD), characterized by a deposition of beta-amyloid peptide (beta/A4) in the brain and in the cerebral microvasculature of affected individuals, is derived from its precursor protein (beta APP) via proteolytic processing by enzyme(s) which have not yet been characterized or localized. Since platelets carry APP in one of their ... More
Regulatory volume decrease in HL-60 cells: importance of rapid changes in permeability of Cl- and organic solutes.
AuthorsHallows KR, Knauf PA
JournalAm J Physiol
PubMed ID7943267
'Results obtained through the use of inhibitors and isotope flux and equilibration techniques indicate that the regulatory volume decrease (RVD) response of human promyelocytic leukemic HL-60 cells occurs largely through the efflux of K+ and Cl- through separate conductive membrane pathways. These "channels" differ pharmacologically and in their modes of ... More
The consequence of sequence alteration of an amphipathic alpha-helical antimicrobial peptide and its diastereomers.
AuthorsPapo N, Oren Z, Pag U, Sahl HG, Shai Y
JournalJ Biol Chem
PubMed ID12110678
'The search for antibiotics with a new mode of action led to numerous studies on antibacterial peptides. Most of the studies were carried out with l-amino acid peptides possessing amphipathic alpha-helix or beta-sheet structures, which are known to be important for biological activities. Here we compared the effect of significantly ... More
Prodigiosins as a new group of H+/Cl- symporters that uncouple proton translocators.
AuthorsSato T, Konno H, Tanaka Y, Kataoka T, Nagai K, Wasserman HH, Ohkuma S
JournalJ Biol Chem
PubMed ID9705273
'We reported previously (Kataoka, T., Muroi, M., Ohkuma, S., Waritani, T., Magae, J., Takatsuki, A., Kondo, S., Yamasaki, M., and Nagai, K. (1995) FEBS Lett. 359, 53-59) that prodigiosin 25-C uncoupled vacuolar H+-ATPase, inhibited vacuolar acidification, and affected glycoprotein processing. In the present study we show that prodigiosins (prodigiosin, metacycloprodigiosin, ... More
Use of fluorescent probes to monitor propranolol effects on the murine splenic lymphocyte.
AuthorsAudus KL, Johnson DL, Gordon MA
JournalJ Immunopharmacol
PubMed ID7184964
'Recent studies indicate that propranolol suppresses the normal murine lymphocyte mitogen dose-response(1) and that propranolol has a high affinity for the beta-adrenergic binding site on the murine lymphocyte(2). Since concentrations which suppress the lymphocyte mitogen dose-response are much greater than the Kd for the binding site, it may be suggested ... More
Glucocorticoid stimulation of ileal Na+ absorptive cell brush border Na+/H+ exchange and association with an increase in message for NHE-3, an epithelial Na+/H+ exchanger isoform.
AuthorsYun CH, Gurubhagavatula S, Levine SA, Montgomery JL, Brant SR, Cohen ME, Cragoe EJ, Pouyssegur J, Tse CM, Donowitz M
JournalJ Biol Chem
PubMed ID8380155
'Methylprednisolone stimulates rabbit ileal neutral NaCl absorption; and aminoglutethimide, which decreases glucocorticoid levels, decreases NaCl absorption. Studies were carried out to determine the mechanism of these effects and to determine which members of the gene family of mammalian Na+/H+ exchangers were involved. Rabbits were treated subcutaneously with methylprednisolone (40 mg ... More
Effect of complement proteins C5b-9 on blood platelets. Evidence for reversible depolarization of membrane potential.
AuthorsWiedmer T, Sims PJ
JournalJ Biol Chem
PubMed ID4008487
'The carbocyanine dye 3,3''-dipropylthiodicarbocyanine iodide has been used to investigate changes in membrane potential (Em) which occur upon binding of complement proteins C5b-9 to the plasma membrane of blood platelets. Gel-filtered platelets exposed to C5b6 and C7 in serum-free medium show no change in Em from that of controls, as ... More
Fluorimetric determination of the resting potential changes associated with the chemotactic response in Paramecium.
AuthorsDoughty MJ, Dodd GH
JournalBiochim Biophys Acta
PubMed ID999871
'(1) Evidence is presented which indicates that the carbocyanine dye (3,3''-dipropyl thiadicarbocyanine) can be used as a spectroscopic probe for monitoring the resting potential across tha plasma membrane of the ciliated protozoan Paramecium. (2) The dye at low concentrations (less than or equal to 1 muM) does not affect either ... More
Cl-/HCO-3 antiporter in red cell ghosts: a kinetic assessment with fluorescent probes.
AuthorsLondon RD, Lipkowitz MS, Abramson RG
JournalAm J Physiol
PubMed ID3034076
'The pH sensitive fluorescent probe acridine orange and membrane potential-sensitive fluorescent probe acridine orange and membrane potential-sensitive fluorescent probe 3,3''-dipropylthiadicarbocyanine iodide were used to evaluate the Cl-/HCO-3 antiporter and proton and potassium conductances, respectively, in human red blood cell ghosts. Acidic, chloride-loaded ghosts alkalinized rapidly in pH 8.5 chloride-free media. ... More
Lysosomal cystine transport. Effect of intralysosomal pH and membrane potential.
AuthorsSmith ML, Greene AA, Potashnik R, Mendoza SA, Schneider JA
JournalJ Biol Chem
PubMed ID2948955
'The regulation of lysosomal cystine transport was studied using cystine dimethyl ester-loaded lysosomes isolated from human diploid fibroblasts. Net efflux from normal fibroblast lysosomes was compared to that from lysosomes of cystinotic fibroblasts, which contain an inherited mutation decreasing lysosomal cystine transport. This exodus of cystine from normal fibroblast lysosomes ... More
Thrombocidins, microbicidal proteins from human blood platelets, are C-terminal deletion products of CXC chemokines.
AuthorsKrijgsveld J, Zaat SA, Meeldijk J, van Veelen PA, Fang G, Poolman B, Brandt E, Ehlert JE, Kuijpers AJ, Engbers GH, Feijen J, Dankert J
JournalJ Biol Chem
PubMed ID10877842
'Antibacterial proteins are components of the innate immune system found in many organisms and produced by a variety of cell types. Human blood platelets contain a number of antibacterial proteins in their alpha-granules that are released upon thrombin activation. The present study was designed to purify these proteins obtained from ... More
Accurate flow cytometric membrane potential measurement in bacteria using diethyloxacarbocyanine and a ratiometric technique.
AuthorsNovo D, Perlmutter NG, Hunt RH, Shapiro HM
JournalCytometry
PubMed ID10554181
'BACKGROUND: Membrane potential (MP) plays a critical role in bacterial physiology. Existing methods for MP estimation by flow cytometry are neither accurate nor precise, due in part to the heterogeneity of size of the particles analyzed. The ratio of a size- and MP-sensitive measurement, and an MP-independent, size-sensitive measurement, should ... More
Phospholipid subclass-specific alterations in the kinetics of ion transport across biologic membranes.
AuthorsChen X, Gross RW
JournalBiochemistry
PubMed ID7947788
'Although the predominance of plasmalogens in electrically-active membranes (e.g., sarcolemma) is well-known, identification of the molecular mechanisms through which the vinyl ether linkage facilitates electrophysiologic function has remained elusive. Herein we demonstrate that the kinetics of both carrier-mediated (i.e., valinomycin) and passive ion transport are substantially different in membranes comprised ... More
Transmembrane potential measurement with carbocyanine dye diS-C3-(5): fast fluorescence decay studies.
AuthorsSíp M, Herman P, Plásek J, Hrouda V
JournalJ Photochem Photobiol B
PubMed ID2107293
'The mechanism of carbocyanine dye diS-C3-(5) fluorescence intensity variations with transmembrane potential changes has been studied using time-resolved fluorescence spectroscopy. Clear evidence is given of the transmembrane-potential-dependent partition of the dye among various sites with different fluorescence lifetimes. It was found that fluorescence decay profiles reflect the transmembrane potential changes.' ... More
The energetics of D-fucose transport in Saccharomyces fragilis. The influence of the protonmotive force on sugar accumulation.
AuthorsVan den Broek PJ, Christianse K, Van Steveninck J
JournalBiochim Biophys Acta
PubMed ID7171593
'The protonmotive force in Saccharomyces fragilis has been estimated under various experimental conditions. The transmembrane potential has been monitored with tetraphenylphosphonium and 3,3''-dipropylthiadicarbocyanine. The distribution ratio of these cations between intracellular and extracellular water appeared to be governed by the electrical potential difference across the membrane of this yeast strain. ... More
Parasporin-1, a novel cytotoxic protein from Bacillus thuringiensis, induces Ca2+ influx and a sustained elevation of the cytoplasmic Ca2+ concentration in toxin-sensitive cells.
AuthorsKatayama H, Kusaka Y, Yokota H, Akao T, Kojima M, Nakamura O, Mekada E, Mizuki E
JournalJ Biol Chem
PubMed ID17204466
'Parasporin-1 is a novel non-insecticidal inclusion protein from Bacillus thuringiensis that is cytotoxic to specific mammalian cells. In this study, we investigated the effects of parasporin-1 on toxin-sensitive cell lines to elucidate the cytotoxic mechanism of parasporin-1. Parasporin-1 is not a membrane pore-forming toxin as evidenced by measurements of lactate ... More
Donnan potential in sarcoplasmic reticulum vesicles measured by using a fluorescent cyanine dye.
AuthorsYamamoto N, Kasai M
JournalJ Biochem (Tokyo)
PubMed ID7462188
'The Donnan potential in sarcoplasmic reticulum vesicles prepared from rabbit skeletal muscle was measured by using a fluorescent cyanine dye, diS-C3-(5) (3,3''-dipropylthiadicarbocyanine iodide). The Donnan potential is caused by the existence of negative fixed charges inside the vesicles. On average, sarcoplasmic reticulum vesicles have internal negative fixed charges of 420 ... More
Functional reconstitution of membrane proteins in monolayer liposomes from bipolar lipids of Sulfolobus acidocaldarius.
'Membranes of Sulfolobus acidocaldarius, an extreme thermophilic archaebacterium, are composed of unusual bipolar lipids. They consist of macrocyclic tetraethers with two polar heads linked by two hydrophobic C40 phytanyl chains which are thought to be arranged as a monolayer in the cytoplasmic membrane. Fractionation of a total lipid-extract from S. ... More
Quantitative measurement of membrane fusions induced by calcium and polyethylene glycol using the porin function.
AuthorsYoshihara E, Nakae T
JournalFEBS Lett
PubMed ID6319190
Changes in potassium permeability and membrane potential of bovine red blood cells estimated by the use of a dimerizing fluorescence probe.
AuthorsHanda T, Sehara C, Nakagaki M
JournalChem Pharm Bull (Tokyo)
PubMed ID6627507
New evaluation method for viability of mitochondria in liver graft using fluorescent dye.
AuthorsOhkohchi N, Shibuya H, Seya K, Satomi S, Taguchi Y, Mori S
JournalTransplant Proc
PubMed ID8266517
The effect of the fluorescent probe, 3,3'-dipropylthiodicarbocyanine iodide, on the membrane potential of Ehrlich ascites tumor cells.
AuthorsSmith TC, Robinson SC
JournalBiochem Biophys Res Commun
PubMed ID7417285
Effects of prostaglandin D2 on membrane potential in neuroblastoma X glioma hybrid cells as determined with a cyanine dye.
AuthorsKondo K, Shimizu T, Hayaishi O
JournalBiochem Biophys Res Commun
PubMed ID7225118
pH Changes in human erythrocytes reported by 3,3' dipropylthia-dicarbocyanine, diS-C3-(5) [proceedings].
AuthorsHladky SB, Rink TJ
JournalJ Physiol
PubMed ID13208
Correlation between membrane potential changes and superoxide production in human granulocytes stimulated by phorbol myristate acetate. Evidence for defective activation in chronic granulomatous disease.
AuthorsWhitin JC, Chapman CE, Simons ER, Chovaniec ME, Cohen HJ
JournalJ Biol Chem
PubMed ID6243652
Calcium-activated potassium channels in lymphocytes.
AuthorsRink TJ, Deutsch C
JournalCell Calcium
PubMed ID6323008
Mechanism of potential-dependent light absorption changes of lipid bilayer membranes in the presence of cyanine and oxonol dyes.
AuthorsWaggoner AS, Wang CH, Tolles RL
JournalJ Membr Biol
PubMed ID864684
Carbocyanine dyes inhibit Ca-dependent K efflux from human red cell ghosts.