DID 油;DiIC18(5) 油(1,1'-双十八烷基-3,3,3',3'-四甲基吲哚菁高氯酸盐)
Invitrogen17万+抗体限时买二赠一,靶点广,灵活用!
DID 油;DiIC<sub>18</sub>(5) 油(1,1'-双十八烷基-3,3,3',3'-四甲基吲哚菁高氯酸盐)
Invitrogen™

DID 油;DiIC18(5) 油(1,1'-双十八烷基-3,3,3',3'-四甲基吲哚菁高氯酸盐)

发远红外荧光的亲脂性碳氰酸酯 DiD 是一种比 DiI 波长更长的模拟物。其在室温下呈油状,在水中发弱荧光,但在掺入膜中后会发出极其明亮且光稳定的荧光。在脂质环境中,其具有超高的消光系数和较短激发态寿命(∼1 纳秒了解更多信息
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货号数量
D30725 mg
货号 D307
价格(CNY)
3,014.00
飞享价
Ends: 31-Dec-2025
4,085.00
共减 1,071.00 (26%)
Each
添加至购物车
数量:
25 mg
价格(CNY)
3,014.00
飞享价
Ends: 31-Dec-2025
4,085.00
共减 1,071.00 (26%)
Each
添加至购物车
发远红外荧光的亲脂性碳氰酸酯 DiD 是一种比 DiI 波长更长的模拟物。其在室温下呈油状,在水中发弱荧光,但在掺入膜中后会发出极其明亮且光稳定的荧光。在脂质环境中,其具有超高的消光系数和较短激发态寿命(∼1 纳秒)。添加到细胞中后,这种染料就会在细胞膜内侧向扩散。
仅供科研使用。不可用于诊断程序。
规格
颜色红色
检测方法荧光
发射665 nm
激发波长范围644 nm
适用于(设备)荧光显微镜
分子量959.92
数量25 mg
运输条件室温
产品类型DiD 油 DiIC18(5) 油
亚细胞定位细胞膜&脂质, Lipids
Unit SizeEach
内容与储存
室温避光储存。

常见问题解答 (FAQ)

我用DiI一类的亲脂性花青染料进行细胞染色,但是当我试图继续用抗体标记时信号却丢失了。 这是什么原因所致?

由于这些染料插入脂质膜,任何对膜的破坏都将导致染料流失。这包括用Triton X-100之类的去垢剂或是甲醇之类的有机溶剂通透。通透是胞内抗体标记所必需的,但它会导致染料流失。相反,CFDA SE之类活性染料能够共价连接到细胞组分,因此可以在固定和通透之后更好的保留。

亲脂性示踪剂跨膜转运需要多久?FAST亲脂性染料能快多少?

转运过程相当缓慢:在活体组织中大约6 mm/天,在固定组织中更慢。亲脂性羰花青示踪剂从开始给样处扩散到神经元末端,可能需要数天甚至数周的时间。FAST DIO和DiI类似物(具有不饱和烷基尾)可以提高50%左右的转运速度。

我应该使用哪种亲脂性示踪剂(DiO、DiI、DiD等)?

请选择与您现有的激发光源和发射滤光片组/通道兼容的染料。固体、糊和晶体形式的染料可直接施加到组织中的神经元。如需标记培养的细胞或进行显微注射,可采用溶液或固态形式的亲脂性染料。

我想标记两种细胞群体然后进行细胞融合实验,哪种试剂最适合来成像?

亲脂性花青染料非常适合此类测定,因为它们会掺入到细胞膜中,随着细胞融合之后共用细胞膜,染料也随之共享。例如,一个细胞群体可以用DiI(橙红色)进行标记,而另一细胞群可以用DIO(绿色)进行标记,当细胞融合时合并的颜色为黄色(使用双带通滤光片成像)。

我想用核酸染料来追踪我的细胞,比如DAPI或者Hoechst染料。您有什么建议吗?

这是不推荐的。这些染料与DNA和RNA的结合会影响核酸的正常功能,扰乱转录和增殖。诸如CellTracker染料或Qtracker试剂在不严重扰乱细胞正常活动的条件下对其进行追踪。如果您仍需要使用核酸染料进行标记且细胞是哺乳动物和非血液来源的话,CellLight 细胞核试剂可通过瞬时转染进入细胞,在核表达蛋白上表达GFP或RFP长达数天而不影响其功能。

引用和文献 (69)

引用和文献
Abstract
Simultaneous measurement of RBC velocity, flux, hematocrit and shear rate in vascular networks.
Authors:Kamoun WS, Chae SS, Lacorre DA, Tyrrell JA, Mitre M, Gillissen MA, Fukumura D, Jain RK, Munn LL,
Journal:Nat Methods
PubMed ID:20581828
Not all tumor vessels are equal. Tumor-associated vasculature includes immature vessels, regressing vessels, transport vessels undergoing arteriogenesis and peritumor vessels influenced by tumor growth factors. Current techniques for analyzing tumor blood flow do not discriminate between vessel subtypes and only measure average changes from a population of dissimilar vessels. We ... More
Dynamics of a chemoattractant receptor in living neutrophils during chemotaxis.
Authors:Servant G, Weiner OD, Neptune ER, Sedat JW, Bourne HR
Journal:Mol Biol Cell
PubMed ID:10198064
'Persistent directional movement of neutrophils in shallow chemotactic gradients raises the possibility that cells can increase their sensitivity to the chemotactic signal at the front, relative to the back. Redistribution of chemoattractant receptors to the anterior pole of a polarized neutrophil could impose asymmetric sensitivity by increasing the relative strength ... More
Regulation of C-cadherin function during activin induced morphogenesis of Xenopus animal caps.
Authors:Brieher WM, Gumbiner BM
Journal:J Cell Biol
PubMed ID:8034750
'Treatment of Xenopus animal pole tissue with activin results in the induction of mesodermal cell types and a dramatic elongation of the tissue. The morphogenetic movements involved in the elongation appear similar to those in normal gastrulation, which is driven by cell rearrangement and cell intercalations. We have used this ... More
Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy.
Authors:Evans CL, Potma EO, Puoris'haag M, Côté D, Lin CP, Xie XS
Journal:Proc Natl Acad Sci U S A
PubMed ID:16263923
'Imaging living organisms with molecular selectivity typically requires the introduction of specific labels. Many applications in biology and medicine, however, would significantly benefit from a noninvasive imaging technique that circumvents such exogenous probes. In vivo microscopy based on vibrational spectroscopic contrast offers a unique approach for visualizing tissue architecture with ... More
In vivo migration of dendritic cells differentiated in vitro: a chimpanzee model.
Authors:Barratt-Boyes SM, Watkins SC, Finn OJ
Journal:J Immunol
PubMed ID:9144465
'Dendritic cells with potent Ag-presenting function can be propagated from peripheral blood using recombinant cytokines, and these cells have potential usefulness as immunotherapeutic agents in the treatment of cancer and other disease states. However, it is not known if these in vitro differentiated dendritic cells have the capacity to migrate ... More