Using fluorescent dyes for fate mapping, lineage analysis, and axon tracing in the chick embryo.
AuthorsClarke JD
JournalMethods in molecular biology (Clifton, N.J.)
PubMed ID19030810
Improved retrograde axonal transport and subsequent visualization of tetramethylrhodamine (TMR) -dextran amine by means of an acidic injection vehicle and antibodies against TMR.
AuthorsKaneko T, Saeki K, Lee T, Mizuno N
JournalJ Neurosci Methods
PubMed ID8740593
'We studied the ability of various dextran amines (DA) to retrogradely label cortical neurons to the full extent of their dendritic configurations. Corticothalamic neurons were labeled by pressure injection of DA into the ventrobasal thalamic nuclei of the rat brain. Of fluorescein-, Texas Red-, Cascade Blue- and tetramethylrhodamine (TMR)-DAs of ... More
Direct pathway from early/recycling endosomes to the Golgi apparatus revealed through the study of shiga toxin B-fragment transport.
AuthorsMallard F, Antony C, Tenza D, Salamero J, Goud B, Johannes L
JournalJ Cell Biol
PubMed ID9817755
'Shiga toxin and other toxins of this family can escape the endocytic pathway and reach the Golgi apparatus. To synchronize endosome to Golgi transport, Shiga toxin B-fragment was internalized into HeLa cells at low temperatures. Under these conditions, the protein partitioned away from markers destined for the late endocytic pathway ... More
A novel dynamin-like protein associates with cytoplasmic vesicles and tubules of the endoplasmic reticulum in mammalian cells.
AuthorsYoon Y, Pitts KR, Dahan S, McNiven MA
JournalJ Cell Biol
PubMed ID9472031
'Dynamins are 100-kilodalton guanosine triphosphatases that participate in the formation of nascent vesicles during endocytosis. Here, we have tested if novel dynamin-like proteins are expressed in mammalian cells to support vesicle trafficking processes at cytoplasmic sites distinct from the plasma membrane. Immunological and molecular biological methods were used to isolate ... More
A role for phosphoinositide 3-kinase in the completion of macropinocytosis and phagocytosis by macrophages.
AuthorsAraki N, Johnson MT, Swanson JA
JournalJ Cell Biol
PubMed ID8947549
'Phosphoinositide 3-kinase (PI 3-kinase) has been implicated in growth factor signal transduction and vesicular membrane traffic. It is thought to mediate the earliest steps leading from ligation of cell surface receptors to increased cell surface ruffling. We show here that inhibitors of PI 3-kinase inhibit endocytosis in macrophages, not by ... More
Electroporation loading of calcium-sensitive dyes into the CNS.
AuthorsBonnot A, Mentis GZ, Skoch J, O'Donovan MJ
JournalJ Neurophysiol
PubMed ID15509647
'Calcium imaging of neural network function has been limited by the extent of tissue labeled or the time taken for labeling. We now describe the use of electroporation-an established technique for transfecting cells with genes-to load neurons with calcium-sensitive dyes in the isolated spinal cord of the neonatal mouse in ... More
Kinesin dependent, rapid, bi-directional transport of ER sub-compartment in dendrites of hippocampal neurons.
AuthorsBannai H, Inoue T, Nakayama T, Hattori M, Mikoshiba K
JournalJ Cell Sci
PubMed ID14676272
'Although spatially restricted Ca2+ release from the endoplasmic reticulum (ER) through intracellular Ca2+ channels plays important roles in various neuronal activities, the accurate distribution and dynamics of ER in the dendrite of living neurons still remain unknown. To elucidate these, we expressed fluorescent protein-tagged ER proteins in cultured mouse hippocampal ... More
Retinal ganglion cell degeneration is topological but not cell type specific in DBA/2J mice.
AuthorsJakobs TC, Libby RT, Ben Y, John SW, Masland RH
JournalJ Cell Biol
PubMed ID16247030
Using a variety of double and triple labeling techniques, we have reevaluated the death of retinal neurons in a mouse model of hereditary glaucoma. Cell-specific markers and total neuron counts revealed no cell loss in any retinal neurons other than the ganglion cells. Within the limits of our ability to ... More
Fast axonal diffusion of 3000 molecular weight dextran amines.
AuthorsFritzsch B
JournalJ Neurosci Methods
PubMed ID7506342
The distances of anterograde and retrograde axonal movement per hour were examined for dextran amines of 3000, 10,000 and 40,000 molecular weights (MW) conjugated to different fluorochromes (Cascade blue, fluorescein, tetramethylrhodamine, Texas red) or to biotin. Lateral line nerves of Xenopus laevis tadpoles were used as an easily accessible test ... More
Dynamin-mediated internalization of caveolae.
AuthorsHenley JR, Krueger EW, Oswald BJ, McNiven MA
JournalJ Cell Biol
PubMed ID9531550
The dynamins comprise an expanding family of ubiquitously expressed 100-kD GTPases that have been implicated in severing clathrin-coated pits during receptor-mediated endocytosis. Currently, it is unclear whether the different dynamin isoforms perform redundant functions or participate in distinct endocytic processes. To define the function of dynamin II in mammalian epithelial ... More
Role of COPI in phagosome maturation.
AuthorsBotelho RJ, Hackam DJ, Schreiber AD, Grinstein S
JournalJ Biol Chem
PubMed ID10748188
Phagosomes mature by sequentially fusing with endosomes and lysosomes. Vesicle budding is presumed to occur concomitantly, mediating the retrieval of plasmalemmal components and the regulation of phagosomal size. We analyzed whether fission of vesicles from phagosomes requires COPI, a multimeric complex known to be involved in budding from the Golgi ... More
Variable Branching Characteristics of Peripheral Taste Neurons Indicates Differential Convergence.
PDGF-B Is Required for Development of the Glymphatic System.
Authors
JournalCell Rep
PubMed ID30865886
Multispectral four-dimensional imaging reveals that evoked activity modulates peripheral arborization and the selection of plane-polarized targets by sensory neurons.
Authors
JournalDevelopment
PubMed ID20430744
Interhemispheric connections between olfactory bulbs improve odor detection.
Authors
JournalPLoS Biol
PubMed ID32310946
Cytotoxic Activity of LLO Y406A Is Targeted to the Plasma Membrane of Cancer Urothelial Cells.
Authors
JournalInt J Mol Sci
PubMed ID33805017
Transcriptomes and neurotransmitter profiles of classes of gustatory and somatosensory neurons in the geniculate ganglion.
Authors
JournalNat Commun
PubMed ID28970527
Targeting Glioma Stem Cell-Derived Pericytes Disrupts the Blood-Tumor Barrier and Improves Chemotherapeutic Efficacy.
Authors
JournalCell Stem Cell
PubMed ID29100012
Organization of primate amygdalar-thalamic pathways for emotions.
Authors
JournalPLoS Biol
PubMed ID32106269
Combined Rehabilitation Promotes the Recovery of Structural and Functional Features of Healthy Neuronal Networks after Stroke.