Using fluorescent dyes for fate mapping, lineage analysis, and axon tracing in the chick embryo.
AuthorsClarke JD
JournalMethods in molecular biology (Clifton, N.J.)
PubMed ID19030810
Improved retrograde axonal transport and subsequent visualization of tetramethylrhodamine (TMR) -dextran amine by means of an acidic injection vehicle and antibodies against TMR.
AuthorsKaneko T, Saeki K, Lee T, Mizuno N
JournalJ Neurosci Methods
PubMed ID8740593
'We studied the ability of various dextran amines (DA) to retrogradely label cortical neurons to the full extent of their dendritic configurations. Corticothalamic neurons were labeled by pressure injection of DA into the ventrobasal thalamic nuclei of the rat brain. Of fluorescein-, Texas Red-, Cascade Blue- and tetramethylrhodamine (TMR)-DAs of ... More
Beyond the olfactory bulb: an odotopic map in the forebrain.
AuthorsNikonov AA, Finger TE, Caprio J
JournalProc Natl Acad Sci U S A
PubMed ID16339016
'We report electrophysiological evidence that a simple odotopy, the spatial mapping of different odorants, is maintained above the level of the olfactory bulb (OB). Three classes of biologically relevant odorants for fish are processed in distinct regions of the forebrain (FB) in the channel catfish. Feeding cues, mainly amino acids ... More
Bringing up the rear: new premotor interneurons add regional complexity to a segmentally distributed motor pattern.
AuthorsWenning A, Norris BJ, Doloc-Mihu A, Calabrese RL,
JournalJ Neurophysiol
PubMed ID21775711
'Central pattern generators (CPGs) pace and pattern many rhythmic activities. We have uncovered a new module in the heartbeat CPG of leeches that creates a regional difference in this segmentally distributed motor pattern. The core CPG consists of seven identified pairs and one unidentified pair of heart interneurons of which ... More
Receptor-dependent and -independent axonal retrograde transport of poliovirus in motor neurons.
AuthorsOhka S, Sakai M, Bohnert S, Igarashi H, Deinhardt K, Schiavo G, Nomoto A,
JournalJ Virol
PubMed ID19244317
'Poliovirus (PV), when injected intramuscularly into the calf, is incorporated into the sciatic nerve and causes an initial paralysis of the inoculated limb in transgenic (Tg) mice carrying the human PV receptor (hPVR/CD155) gene. We have previously demonstrated that a fast retrograde axonal transport process is required for PV dissemination ... More
Cyclic AMP-induced repair of zebrafish spinal circuits.
AuthorsBhatt DH, Otto SJ, Depoister B, Fetcho JR
JournalScience
PubMed ID15247482
Neurons in the human central nervous system (CNS) are unable to regenerate, as a result of both an inhibitory environment and their inherent inability to regrow. In contrast, the CNS environment in fish is permissive for growth, yet some neurons still cannot regenerate. Fish thus offer an opportunity to study ... More
Early development of ocular dominance columns.
AuthorsCrowley JC, Katz LC
JournalScience
PubMed ID11082053
The segregation of lateral geniculate nucleus (LGN) axons into ocular dominance columns is believed to involve a prolonged, activity-dependent sorting process. However, visualization of early postnatal ferret LGN axons by direct LGN tracer injections revealed segregated ocular dominance columns <7 days after innervation of layer 4. These early columns were ... More
Knockdown of the survival motor neuron (Smn) protein in zebrafish causes defects in motor axon outgrowth and pathfinding.
AuthorsMcWhorter ML, Monani UR, Burghes AH, Beattie CE
JournalJ Cell Biol
PubMed ID12952942
Spinal muscular atrophy (SMA) is an autosomal recessive disorder characterized by a loss of alpha motoneurons in the spinal cord. SMA is caused by low levels of the ubiquitously expressed survival motor neuron (Smn) protein. As it is unclear how low levels of Smn specifically affect motoneurons, we have modeled ... More
Selective release of molecules from Weibel-Palade bodies during a lingering kiss.
AuthorsBabich V, Meli A, Knipe L, Dempster JE, Skehel P, Hannah MJ, Carter T,
JournalBlood
PubMed ID18252862
Exocytosis of specialized endothelial cell secretory organelles, Weibel-Palade bodies (WPBs), is thought to play an important role in regulating hemostasis and intravascular inflammation. The major WPB core proteins are Von Willebrand factor (VWF) and its propolypeptide (Proregion), constituting more than 95% of the content. Although the composition of the WPBs ... More
Retinal ganglion cell degeneration is topological but not cell type specific in DBA/2J mice.
AuthorsJakobs TC, Libby RT, Ben Y, John SW, Masland RH
JournalJ Cell Biol
PubMed ID16247030
Using a variety of double and triple labeling techniques, we have reevaluated the death of retinal neurons in a mouse model of hereditary glaucoma. Cell-specific markers and total neuron counts revealed no cell loss in any retinal neurons other than the ganglion cells. Within the limits of our ability to ... More
Asymmetric p38 activation in zebrafish: its possible role in symmetric and synchronous cleavage.
AuthorsFujii R, Yamashita S, Hibi M, Hirano T
JournalJ Cell Biol
PubMed ID10995439
Cleavage is one of the initial steps of embryogenesis, and is characterized by a series of symmetric and synchronous cell divisions. We showed that p38 MAP kinase (p38) is asymmetrically activated on one side of the blastodisc during the early cleavage period in zebrafish (Danio rerio) embryos. When a dominant ... More
Direct membrane retrieval into large vesicles after exocytosis in sea urchin eggs.
AuthorsWhalley T, Terasaki M, Cho MS, Vogel SS
JournalJ Cell Biol
PubMed ID8522582
At fertilization in sea urchin eggs, elevated cytosolic Ca2+ leads to the exocytosis of 15,000-18,000 1.3-microns-diam cortical secretory granules to form the fertilization envelope. Cortical granule exocytosis more than doubles the surface area of the egg. It is thought that much of the added membrane is retrieved by subsequent endocytosis. ... More
Intracellular labeling of cat spinal neurons using a tetramethylrhodamine-dextran amine conjugate.
AuthorsCarr PA, Noga BR, Nance DM, Jordan LM
JournalBrain Res Bull
PubMed ID7521780
Tetramethylrhodamine-dextran is a highly fluorescent neuroanatomical tracer that, in its 10,000 MW form, has seen widespread use as a sensitive anterograde tract-tracing label. We report here the use of a lower molecular weight tetramethylrhodamine-dextran (3000 MW; Molecular Probes, OR) as an in vivo intracellular marker of locomotor-related spinal neurons. In ... More
Fast axonal diffusion of 3000 molecular weight dextran amines.
AuthorsFritzsch B
JournalJ Neurosci Methods
PubMed ID7506342
The distances of anterograde and retrograde axonal movement per hour were examined for dextran amines of 3000, 10,000 and 40,000 molecular weights (MW) conjugated to different fluorochromes (Cascade blue, fluorescein, tetramethylrhodamine, Texas red) or to biotin. Lateral line nerves of Xenopus laevis tadpoles were used as an easily accessible test ... More
Phrenic motoneuron morphology during rapid diaphragm muscle growth.
In the adult rat, there is a general correspondence between the sizes of motoneurons, motor units, and muscle fibers that has particular functional importance in motor control. During early postnatal development, after the establishment of singular innervation, there is rapid growth of diaphragm muscle (Dia(m)) fibers. In the present study, ... More
Organization of geniculate and trigeminal ganglion cells innervating single fungiform taste papillae: a study with tetramethylrhodamine dextran amine labeling.
AuthorsWhitehead MC, Ganchrow JR, Ganchrow D, Yao B
JournalNeuroscience
PubMed ID10473258
Single gustatory nerve fibers branch and innervate several taste buds. In turn, individual taste buds may receive innervation from numerous gustatory nerve fibers. To evaluate the pattern of sensory innervation of fungiform papilla-bearing taste buds, we used iontophoretic fluorescent injection to retrogradely label the fibers that innervate single taste papillae ... More
Efficacy of seven retrograde tracers, compared in multiple-labelling studies of feline motoneurones.
AuthorsRichmond FJ, Gladdy R, Creasy JL, Kitamura S, Smits E, Thomson DB
JournalJ Neurosci Methods
PubMed ID7527476
The labelling efficacies of 7 retrograde tracers were evaluated following cut nerve exposure or intramuscular injection into the serially compartmentalized neck muscle, biventer cervicis. Tested tracers included Fast Blue (FB), Fluorogold (FG), dextran conjugated to fluorescein (FD), dextran conjugated to rhodamine (Fluororuby (FR), 3000 and 10,000 MW), fluorescent latex microspheres, ... More
Coupling between serotoninergic and noradrenergic neurones and gamma-motoneurones in the cat.
AuthorsGladden MH, Maxwell DJ, Sahal A, Jankowska E
JournalJ Physiol
PubMed ID10970424
Noradrenaline is known to suppress transmission from group II muscle afferents when locally applied to gamma-motoneurones, and serotonin (5-HT) facilitates the transmission. The purpose of this investigation was to search for evidence of monoaminergic innervation of gamma-motoneurones. Eight gamma-motoneurones were labelled with rhodamine-dextran, and 50 micrometer thick sagittal sections of ... More
HIF-1α is involved in blood-brain barrier dysfunction and paracellular migration of bacteria in pneumococcal meningitis.
Authors
JournalActa Neuropathol
PubMed ID32529267
P7C3-A20 treatment one year after TBI in mice repairs the blood-brain barrier, arrests chronic neurodegeneration, and restores cognition.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID33087571
Neonatal insulin action impairs hypothalamic neurocircuit formation in response to maternal high-fat feeding.
Authors
JournalCell
PubMed ID24462248
Intra-neuronal Competition for Synaptic Partners Conserves the Amount of Dendritic Building Material.
Authors
JournalNeuron
PubMed ID28132832
Information flow through neural circuits for pheromone orientation.
Authors
JournalNat Commun
PubMed ID25533353
Functionally distinct POMC-expressing neuron subpopulations in hypothalamus revealed by intersectional targeting.
Authors
JournalNat Neurosci
PubMed ID34002087
GLP-1 Receptor Signaling in Astrocytes Regulates Fatty Acid Oxidation, Mitochondrial Integrity, and Function.
Authors
JournalCell Metab
PubMed ID32433922
Transcriptomes and neurotransmitter profiles of classes of gustatory and somatosensory neurons in the geniculate ganglion.
Authors
JournalNat Commun
PubMed ID28970527
Gradual Suppression of Transcytosis Governs Functional Blood-Retinal Barrier Formation.
Authors
JournalNeuron
PubMed ID28334606
Precise Temporal Regulation of Molecular Diffusion within Dendritic Spines by Actin Polymers during Structural Plasticity.
AuthorsObashi K, Matsuda A, Inoue Y, Okabe S
JournalCell Rep
PubMed ID31042476
The biochemical transduction of excitatory synaptic signals occurs in the cytoplasm within dendritic spines. The associated reaction kinetics are shaped by the mobility of the signaling molecules; however, accurate monitoring of diffusional events within the femtoliter-sized spine structures has not yet been demonstrated. Here, we applied two-photon fluorescence correlation spectroscopy ... More
Regenerative Failure Following Rat Neonatal Chorda Tympani Transection is Associated with Geniculate Ganglion Cell Loss and Terminal Field Plasticity in the Nucleus of the Solitary Tract.
AuthorsMartin LJ, Lane AH, Samson KK, Sollars SI
JournalNeuroscience
PubMed ID30684590
Neural insult during development results in recovery outcomes that vary dependent upon the system under investigation. Nerve regeneration does not occur if the rat gustatory chorda tympani nerve is sectioned (CTX) during neonatal (=P10) development. It is unclear how chorda tympani soma and terminal fields are affected after neonatal CTX. ... More
Calorie-rich diets induce hyperphagia and promote obesity, although the underlying mechanisms remain poorly defined. We find that short-term high-fat-diet (HFD) feeding of mice activates prepronociceptin (PNOC)-expressing neurons in the arcuate nucleus of the hypothalamus (ARC). PNOC ... More
Synthesis and In Vitro Photodynamic Activity of Cationic Boron Dipyrromethene-Based Photosensitizers against Methicillin-Resistant
AuthorsDharmaratne P, Wong RCH, Wang J, Lo PC, Wang B, Chan BCL, Lau KM, Lau CBS, Fung KP, Ip M, Ng DKP
JournalBiomedicines
PubMed ID32485946
A series of cationic boron dipyrromethene (BODIPY) derivatives were synthesized and characterized with various spectroscopic methods. Having the ability to generate singlet oxygen upon irradiation, these compounds could potentially serve as photosensitizers for antimicrobial photodynamic therapy. Of the five BODIPYs being examined, the dicationic aza-BODIPY analogue (compound ... More
Multiple Rhythm-Generating Circuits Act in Tandem with Pacemaker Properties to Control the Start and Speed of Locomotion.
AuthorsSong J, Pallucchi I, Ausborn J, Ampatzis K, Bertuzzi M, Fontanel P, Picton LD, El Manira A
JournalNeuron
PubMed ID31982322
In vertebrates, specific command centers in the brain can selectively drive slow-explorative or fast-speed locomotion. However, it remains unclear how the locomotor central pattern generator (CPG) processes descending drive into coordinated locomotion. Here, we reveal, in adult zebrafish, a logic of the V2a interneuron rhythm-generating circuits involving recurrent and hierarchical ... More