Direct measurement of coupling between dendritic spines and shafts.
AuthorsSvoboda K, Tank DW, Denk W
JournalScience
PubMed ID8614831
'Characterization of the diffusional and electrotonic coupling of spines to the dendritic shaft is crucial to understanding neuronal integration and synaptic plasticity. Two-photon photobleaching and photorelease of fluorescein dextran were used to generate concentration gradients between spines and shafts in rat CA1 pyramidal neurons. Diffusional reequilibration was monitored with two-photon ... More
Turning of nerve growth cones induced by localized increases in intracellular calcium ions.
AuthorsZheng JQ
JournalNature
PubMed ID10638759
'Guidance of developing axons involves turning of the motile tip, the growth cone, in response to a variety of extracellular cues. Little is known about the intracellular mechanism by which the directional signal is transduced. Ca2+ is a key second messenger in growth cone extension and has been implicated in ... More
Axonal transport of tubulin in Ti1 pioneer neurons in situ.
AuthorsSabry J, O'Connor TP, Kirschner MW
JournalNeuron
PubMed ID7541635
'In neurons, tubulin is synthesized only in the cell body or dendrites, yet the growing axon requires a steady supply of this protein at the growth cone. Hence, some mechanism must exist to move tubulin from the cell body to the growth cone. Transport could conceivably occur by simple diffusion, ... More
Photolysis of caged phosphatidic acid induces flagellar excision in Chlamydomonas.
AuthorsGoedhart J, Gadella TW
JournalBiochemistry
PubMed ID15065870
'Phosphatidic (PtdOH) acid formation is recognized as an important step in numerous signaling pathways in both plants and mammals. To study the role of this lipid in signaling pathways, it is of major interest to be able to increase the amount of this lipid directly. Therefore, "caged" PtdOH was synthesized, ... More
Synapse-specific plasticity and compartmentalized signaling in cerebellar stellate cells.
AuthorsSoler-Llavina GJ, Sabatini BL
JournalNat Neurosci
PubMed ID16680164
'Here we demonstrate that cerebellar stellate cells diffusionally isolate synaptically evoked signals in dendrites and are capable of input-specific synaptic plasticity. Sustained activity of parallel fibers induces a form of long-term depression that requires opening of calcium (Ca(2+))-permeable AMPA-type glutamate receptors (CP-AMPARs) and signaling through class 1 metabotropic glutamate receptors ... More
Photon upmanship: why multiphoton imaging is more than a gimmick.
AuthorsDenk W, Svoboda K
JournalNeuron
PubMed ID9115730
Intercellular calcium signaling and flash photolysis of caged compounds. A sensitive method to evaluate gap junctional coupling.
AuthorsLeybaert L, Sanderson MJ
JournalMethods Mol Biol
PubMed ID11218662
Photoactivation of caged compounds in single living cells: an application to the study of cell locomotion.
AuthorsIshihara A, Gee K, Schwartz S, Jacobson K, Lee J
JournalBiotechniques
PubMed ID9266081
Caged compounds are molecules whose biological function is masked until UV light induces a photo-chemical reaction that converts the molecules into a biologically active state. These probes provide very powerful tools in cell biology research, since the activation can be precisely controlled temporally and spatially by limiting their exposure to ... More
Diffusion coefficients in the lateral intercellular spaces of Madin-Darby canine kidney cell epithelium determined with caged compounds.
AuthorsXia P, Bungay PM, Gibson CC, Kovbasnjuk ON, Spring KR
JournalBiophys J
PubMed ID9635784
The diffusion coefficients of two caged fluorescent dyes were measured in free solution and in the lateral intercellular spaces (LIS) of cultured Madin-Darby canine kidney (MDCK) cells after photoactivation by illumination with a continuous or pulsed UV laser. Both quantitative video imaging and a new photometric method were utilized to ... More
Regional cell movement and tissue patterning in the zebrafish embryo revealed by fate mapping with caged fluorescein.
AuthorsKozlowski DJ, Murakami T, Ho RK, Weinberg ES
JournalBiochem Cell Biol
PubMed ID9551179
Determination of fate maps and cell lineage tracing have previously been carried out in the zebrafish embryo by following the progeny of individual cells injected with fluorescent dyes. We review the information obtained from these experiments and then present an approach to fate mapping and cell movement tracing, utilizing the ... More
Long distance communication between muscarinic receptors and Ca2+ release channels revealed by carbachol uncaging in cell-attached patch pipette.
AuthorsAshby MC, Camello-Almaraz C, Gerasimenko OV, Petersen OH, Tepikin AV
JournalJ Biol Chem
PubMed ID12657637
We have investigated the characteristics of cytosolic Ca2+ signals induced by muscarinic receptor activation of pancreatic acinar cells that reside within intact pancreatic tissue. We show that these cells exhibit global Ca2+ waves and local apical Ca2+ spikes. This is the first evidence for local Ca2+ signaling in undissociated pancreatic ... More
Estimation of the sarcoplasmic reticulum Ca2+ release flux underlying Ca2+ sparks.
AuthorsSoeller C, Cannell MB
JournalBiophys J
PubMed ID11964229
Using a combination of experimental and numerical approaches, we have tested two different approaches to calculating the sarcoplasmic reticulum (SR) Ca2+ release flux, which gives rise to cardiac muscle Ca2+ sparks. By using two-photon excited spot photolysis of DM-Nitrophen, known Ca2+ release flux time courses were generated to provide the ... More
Notochord regulates cardiac lineage in zebrafish embryos.
AuthorsGoldstein AM, Fishman MC
JournalDev Biol
PubMed ID9740662
We focus here upon regulation by the notochord of myocardial cell fate in zebrafish. Myocardial precursors, defined by lineage tracing in the living embryo, are in the lateral plate mesoderm adjacent to the notochord-prechordal plate junction. Interestingly, the anterior end of the notochord corresponds to the posterior extent of the ... More
Diffusion of small solutes in the lateral intercellular spaces of MDCK cell epithelium grown on permeable supports.
AuthorsKovbasnjuk ON, Bungay PM, Spring KR
JournalJ Membr Biol
PubMed ID10811963
The diffusion coefficients of four solutes ranging in molecular weight from 238 to 10,000 in the lateral intercellular spaces (LIS) of cultured kidney cells (MDCK) grown on permeable supports were determined from the spread of fluorescence produced after the release of caged compounds by a pulse from a UV laser. ... More
Mossy fiber-granule cell synapses in the normal and epileptic rat dentate gyrus studied with minimal laser photostimulation.
AuthorsMolnár P, Nadler JV
JournalJ Neurophysiol
PubMed ID10515977
Dentate granule cells become synaptically interconnected in the hippocampus of persons with temporal lobe epilepsy, forming a recurrent mossy fiber pathway. This pathway may contribute to the development and propagation of seizures. The physiology of mossy fiber-granule cell synapses is difficult to characterize unambiguously, because electrical stimulation may activate other ... More
Local photorelease of caged thymosin beta4 in locomoting keratocytes causes cell turning.
AuthorsRoy P, Rajfur Z, Jones D, Marriott G, Loew L, Jacobson K
JournalJ Cell Biol
PubMed ID11381088
The broad aim of this work was to explore the feasibility of using light-directed perturbation techniques to study cell locomotion. Specifically, a caged form of thymosin beta4 (Tbeta4) was photoactivated in a defined local region of locomoting fish scale keratocytes and the resulting perturbation of locomotion was studied. Purified Tbeta4 ... More