Improved retrograde axonal transport and subsequent visualization of tetramethylrhodamine (TMR) -dextran amine by means of an acidic injection vehicle and antibodies against TMR.
AuthorsKaneko T, Saeki K, Lee T, Mizuno N
JournalJ Neurosci Methods
PubMed ID8740593
'We studied the ability of various dextran amines (DA) to retrogradely label cortical neurons to the full extent of their dendritic configurations. Corticothalamic neurons were labeled by pressure injection of DA into the ventrobasal thalamic nuclei of the rat brain. Of fluorescein-, Texas Red-, Cascade Blue- and tetramethylrhodamine (TMR)-DAs of ... More
Intracellular fluid flow in rapidly moving cells.
AuthorsKeren K, Yam PT, Kinkhabwala A, Mogilner A, Theriot JA,
JournalNat Cell Biol
PubMed ID19767741
'Cytosolic fluid dynamics have been implicated in cell motility because of the hydrodynamic forces they induce and because of their influence on transport of components of the actin machinery to the leading edge. To investigate the existence and the direction of fluid flow in rapidly moving cells, we introduced inert ... More
Kinesin dependent, rapid, bi-directional transport of ER sub-compartment in dendrites of hippocampal neurons.
AuthorsBannai H, Inoue T, Nakayama T, Hattori M, Mikoshiba K
JournalJ Cell Sci
PubMed ID14676272
'Although spatially restricted Ca2+ release from the endoplasmic reticulum (ER) through intracellular Ca2+ channels plays important roles in various neuronal activities, the accurate distribution and dynamics of ER in the dendrite of living neurons still remain unknown. To elucidate these, we expressed fluorescent protein-tagged ER proteins in cultured mouse hippocampal ... More
In vivo diffusion analysis with quantum dots and dextrans predicts the width of brain extracellular space.
AuthorsThorne RG, Nicholson C
JournalProc Natl Acad Sci U S A
PubMed ID16567637
'Diffusion within the extracellular space (ECS) of the brain is necessary for chemical signaling and for neurons and glia to access nutrients and therapeutics; however, the width of the ECS in living tissue remains unknown. We used integrative optical imaging to show that dextrans and water-soluble quantum dots with Stokes-Einstein ... More
Intravital multiphoton microscopy of dynamic renal processes.
AuthorsMolitoris BA, Sandoval RM
JournalAm J Physiol Renal Physiol
PubMed ID15883167
'Recent advances in microscopy and optics, computer sciences, and the available fluorophores used to label molecules of interest have empowered investigators to utilize intravital two-photon microscopy to study the dynamic events within the functioning kidney. This emerging technique enables investigators to follow functional and structural alterations with subcellular resolution within ... More
Neural organization and visual processing in the anterior optic tubercle of the honeybee brain.
AuthorsMota T, Yamagata N, Giurfa M, Gronenberg W, Sandoz JC,
JournalJ Neurosci
PubMed ID21832175
The honeybee Apis mellifera represents a valuable model for studying the neural segregation and integration of visual information. Vision in honeybees has been extensively studied at the behavioral level and, to a lesser degree, at the physiological level using intracellular electrophysiological recordings of single neurons. However, our knowledge of visual ... More
Role of Drosophila Rab5 during endosomal trafficking at the synapse and evoked neurotransmitter release.
AuthorsWucherpfennig T, Wilsch-Bräuninger M, González-Gaitán M
JournalJ Cell Biol
PubMed ID12743108
During constitutive endocytosis, internalized membrane traffics through endosomal compartments. At synapses, endocytosis of vesicular membrane is temporally coupled to action potential-induced exocytosis of synaptic vesicles. Endocytosed membrane may immediately be reused for a new round of neurotransmitter release without trafficking through an endosomal compartment. Using GFP-tagged endosomal markers, we monitored ... More
Fast axonal diffusion of 3000 molecular weight dextran amines.
AuthorsFritzsch B
JournalJ Neurosci Methods
PubMed ID7506342
The distances of anterograde and retrograde axonal movement per hour were examined for dextran amines of 3000, 10,000 and 40,000 molecular weights (MW) conjugated to different fluorochromes (Cascade blue, fluorescein, tetramethylrhodamine, Texas red) or to biotin. Lateral line nerves of Xenopus laevis tadpoles were used as an easily accessible test ... More
Dmon1 controls recruitment of Rab7 to maturing endosomes in Drosophila.
AuthorsYousefian J, Troost T, Grawe F, Sasamura T, Fortini M, Klein T
JournalJ Cell Sci
PubMed ID23418349
The small GTPases Rab5 and Rab7 are important organisers of endosome formation and maturation. In addition, they orchestrate the trafficking of cargo through the endosomal pathway. A crucial event during maturation of endosomes is the replacement of the early organiser Rab5 with the late organiser Rab7 in a process called ... More
Fiber pathways and positional changes in efferent perikarya of 2.5- to 7-day chick embryos as revealed with DiI and dextran amines.
AuthorsFritzsch B, Christensen MA, Nichols DH
JournalJ Neurobiol
PubMed ID7506749
The differentiation of facial motoneurons and inner ear (octaval) efferents was examined in chicken embryos by applying DiI or dextran amines to the cut VII/VIII nerve (peripheral label) or to the basal/floor plate of rhombomeres 4/5 (central label). Central labeling found axons of these efferent neurons to leave the brain ... More
Dynamin-mediated internalization of caveolae.
AuthorsHenley JR, Krueger EW, Oswald BJ, McNiven MA
JournalJ Cell Biol
PubMed ID9531550
The dynamins comprise an expanding family of ubiquitously expressed 100-kD GTPases that have been implicated in severing clathrin-coated pits during receptor-mediated endocytosis. Currently, it is unclear whether the different dynamin isoforms perform redundant functions or participate in distinct endocytic processes. To define the function of dynamin II in mammalian epithelial ... More
ORF3a of the COVID-19 virus SARS-CoV-2 blocks HOPS complex-mediated assembly of the SNARE complex required for autolysosome formation.
Authors
JournalDev Cell
PubMed ID33422265
Ubiquitous macropinocytosis in anthozoans.
AuthorsGanot P, Tambutté E, Caminiti-Segonds N, Toullec G, Allemand D, Tambutté S
JournalElife
PubMed ID32039759
'Transport of fluids, molecules, nutrients or nanoparticles through coral tissues are poorly documented. Here, we followed the flow of various tracers from the external seawater to within the cells of all tissues in living animals. After entering the general coelenteric cavity, we show that nanoparticles disperse throughout the tissues via ... More
Visual Input into the Drosophila melanogaster Mushroom Body.
Authors
JournalCell Rep
PubMed ID32937130
Single-trial neural dynamics are dominated by richly varied movements.
Authors
JournalNat Neurosci
PubMed ID31551604
Live imaging reveals the cellular events downstream of SARM1 activation.
Authors
JournalElife
PubMed ID34779400
A Central Catecholaminergic Circuit Controls Blood Glucose Levels during Stress.
Authors
JournalNeuron
PubMed ID28625488
Profiling the neurovascular unit unveils detrimental effects of osteopontin on the blood-brain barrier in acute ischemic stroke.
Authors
JournalActa Neuropathol
PubMed ID35752654
Sensory Discrimination of Blood and Floral Nectar by Aedes aegypti Mosquitoes.
Authors
JournalNeuron
PubMed ID33049200
Calcium Influx through Plasma-Membrane Nanoruptures Drives Axon Degeneration in a Model of Multiple Sclerosis.
Authors
JournalNeuron
PubMed ID30686733
LAP-like non-canonical autophagy and evolution of endocytic vacuoles in pancreatic acinar cells.
Authors
JournalAutophagy
PubMed ID31651224
Multicolor two-photon imaging of in vivo cellular pathophysiology upon influenza virus infection using the two-photon IMPRESS.
Authors
JournalNat Protoc
PubMed ID31996843
Macrophages Facilitate Electrical Conduction in the Heart.
AuthorsHulsmans M, Clauss S, Xiao L, Aguirre AD, King KR, Hanley A, Hucker WJ, Wülfers EM, Seemann G, Courties G, Iwamoto Y, Sun Y, Savol AJ, Sager HB, Lavine KJ, Fishbein GA, Capen DE, Da Silva N, Miquerol L, Wakimoto H, Seidman CE, Seidman JG, Sadreyev RI, Naxerova K, Mitchell RN, Brown D, Libby P, Weissleder R, Swirski FK, Kohl P, Vinegoni C, Milan DJ, Ellinor PT, Nahrendorf M
JournalCell
PubMed ID28431249
Organ-specific functions of tissue-resident macrophages in the steady-state heart are unknown. Here, we show that cardiac macrophages facilitate electrical conduction through the distal atrioventricular node, where conducting cells densely intersperse with elongated macrophages expressing connexin 43. When coupled to spontaneously beating cardiomyocytes via connexin-43-containing gap junctions, cardiac macrophages have a ... More
Glia Accumulate Evidence that Actions Are Futile and Suppress Unsuccessful Behavior.
AuthorsMu Y, Bennett DV, Rubinov M, Narayan S, Yang CT, Tanimoto M, Mensh BD, Looger LL, Ahrens MB
JournalCell
PubMed ID31230713
When a behavior repeatedly fails to achieve its goal, animals often give up and become passive, which can be strategic for preserving energy or regrouping between attempts. It is unknown how the brain identifies behavioral failures and mediates this behavioral-state switch. In larval zebrafish swimming in virtual reality, visual feedback ... More
LC3-Associated Endocytosis Facilitates ß-Amyloid Clearance and Mitigates Neurodegeneration in Murine Alzheimer's Disease.
AuthorsHeckmann BL, Teubner BJW, Tummers B, Boada-Romero E, Harris L, Yang M, Guy CS, Zakharenko SS, Green DR
JournalCell
PubMed ID31257024
The expression of some proteins in the autophagy pathway declines with age, which may impact neurodegeneration in diseases, including Alzheimer's Disease. We have identified a novel non-canonical function of several autophagy proteins in the conjugation of LC3 to Rab5 ... More
Spinal Interneurons With Dual Axon Projections to Knee-Extensor and Hip-Extensor Motor Pools.
AuthorsNguyen KH, Scheurich TE, Gu T, Berkowitz A
JournalFront Neural Circuits
PubMed ID32226362
The central nervous system (CNS) may simplify control of limb movements by activating certain combinations of muscles together, i.e., muscle synergies. Little is known, however, about the spinal cord interneurons that activate muscle synergies by exciting sets of motoneurons for different muscles. The turtle spinal cord, even without brain inputs ... More
Reverse Genetic Approaches to Investigate the Neurobiology of the Cnidarian Sea Anemone Nematostella vectensis.
AuthorsHavrilak JA, Layden MJ
JournalMethods Mol Biol
PubMed ID31552647
The cnidarian sea anemone Nematostella vectensis has grown in popularity as a model system to complement the ongoing work in traditional bilaterian model species (e.g. Drosophila, C. elegans, vertebrate). The driving force behind developing cnidarian model systems is the potential of this group of animals to impact EvoDevo studies aimed ... More
In vitro investigation of hyaluronan-based polymeric micelles for drug delivery into the skin: The internalization pathway.
AuthorsStarigazdová J, Nešporová K, Cepa M, Šínová R, Šmejkalová D, Huerta-Angeles G, Velebný V
JournalEur J Pharm Sci
PubMed ID31783157
In our previous research, we concluded that polymeric micelles based on hyaluronic acid are able to penetrate into the deeper layers of skin tissue. The aim of this work was to characterize the mechanisms involved in the uptake by skin cells, which is important for understanding the influence of the ... More