Monitoring the permeability of the nuclear envelope during the cell cycle.
AuthorsLénárt P, Ellenberg J,
JournalMethods
PubMed ID16343937
'In animal organisms the nuclear envelope (NE) dis-assembles during cell division resulting in complete intermixing of cytoplasmic and nuclear compartments. This leads to the activation of many mitotic enzymes, which were kept away from their substrates or regulators by nuclear or cytoplasmic sequestration in interphase. Nuclear envelope breakdown (NEBD) is ... More
Visualization of the dynamics of synaptic vesicle and plasma membrane proteins in living axons.
AuthorsNakata T, Terada S, Hirokawa N
JournalJ Cell Biol
PubMed ID9456325
Newly synthesized membrane proteins are transported by fast axonal flow to their targets such as the plasma membrane and synaptic vesicles. However, their transporting vesicles have not yet been identified. We have successfully visualized the transporting vesicles of plasma membrane proteins, synaptic vesicle proteins, and the trans-Golgi network residual proteins ... More
Direct membrane retrieval into large vesicles after exocytosis in sea urchin eggs.
AuthorsWhalley T, Terasaki M, Cho MS, Vogel SS
JournalJ Cell Biol
PubMed ID8522582
At fertilization in sea urchin eggs, elevated cytosolic Ca2+ leads to the exocytosis of 15,000-18,000 1.3-microns-diam cortical secretory granules to form the fertilization envelope. Cortical granule exocytosis more than doubles the surface area of the egg. It is thought that much of the added membrane is retrieved by subsequent endocytosis. ... More