A fluorometric approach to local electric field measurements in a voltage-gated ion channel.
AuthorsAsamoah OK, Wuskell JP, Loew LM, Bezanilla F
JournalNeuron
PubMed ID12526775
'Site-specific electrostatic measurements have been limited to soluble proteins purified for in vitro spectroscopic characterization or proteins of known structure; however, comparable measurements have not been made for functional membrane bound proteins. Here, using an electrochromic fluorophore, we describe a method to monitor localized electric field changes in a voltage-gated ... More